Restriction map for
pFN21AB7052
(Linear) (Six-base) MAPSORT of: KIBB7052.seq Check: 7576 from: 1 to: 1617
KIBB7052.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1274 1340 1617
Size: 1274 66 277
Fragments arranged by size:
1274 277 66
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1186 1616 1617
Size: 1186 430 1
Fragments arranged by size:
1186 430 1
AleI CACnn'nnGTG
Cuts at: 0 50 98 1617
Size: 50 48 1519
Fragments arranged by size:
1519 50 48
AlwNI CAG_nnn'CTG
Cuts at: 0 486 778 1617
Size: 486 292 839
Fragments arranged by size:
839 486 292
ApaI G_GGCC'C
Cuts at: 0 887 1529 1550 1617
Size: 887 642 21 67
Fragments arranged by size:
887 642 67 21
ApoI r'AATT_y
Cuts at: 0 310 1617
Size: 310 1307
AvaI C'yCGr_G
Cuts at: 0 10 1617
Size: 10 1607
BanI G'GyrC_C
Cuts at: 0 298 549 666 978 1382 1575 1600 1617
Size: 298 251 117 312 404 193 25 17
Fragments arranged by size:
404 312 298 251 193 117 25 17
BanII G_rGCy'C
Cuts at: 0 10 59 477 887 913 1529 1550 1617
Size: 10 49 418 410 26 616 21 67
Fragments arranged by size:
616 418 410 67 49 26 21 10
BbeI G_GCGC'C
Cuts at: 0 302 1386 1617
Size: 302 1084 231
Fragments arranged by size:
1084 302 231
BbsI GAAGACnn'nnnn_
Cuts at: 0 539 1617
Size: 539 1078
BglI GCCn_nnn'nGGC
Cuts at: 0 89 582 1617
Size: 89 493 1035
Fragments arranged by size:
1035 493 89
Bme1580I G_kGCm'C
Cuts at: 0 554 671 680 887 1045 1529 1550 1578
Size: 554 117 9 207 158 484 21 28
Cuts at: 1578 1617
Size: 39
Fragments arranged by size:
554 484 207 158 117 39 28 21
9
BmrI ACTGGGnnnn_n'
Cuts at: 0 251 1532 1617
Size: 251 1281 85
Fragments arranged by size:
1281 251 85
BmtI G_CTAG'C
Cuts at: 0 157 1617
Size: 157 1460
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 927 1617
Size: 927 690
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 403 508 536 1384 1617
Size: 349 54 105 28 848 233
Fragments arranged by size:
848 349 233 105 54 28
Bpu10I CC'TnA_GC
Cuts at: 0 61 952 1617
Size: 61 891 665
Fragments arranged by size:
891 665 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1234 1617
Size: 1234 383
BsaI GGTCTCn'nnnn_
Cuts at: 0 1539 1617
Size: 1539 78
BsaHI Gr'CG_yC
Cuts at: 0 299 1383 1617
Size: 299 1084 234
Fragments arranged by size:
1084 299 234
BsaWI w'CCGG_w
Cuts at: 0 113 1617
Size: 113 1504
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 376 406 940 970 1617
Size: 376 30 534 30 647
Fragments arranged by size:
647 534 376 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 715 718 721 853 916 1579 1617
Size: 715 3 3 132 63 663 38
Fragments arranged by size:
715 663 132 63 38 3 3
BseYI C'CCAG_C
Cuts at: 0 87 445 855 906 1221 1617
Size: 87 358 410 51 315 396
Fragments arranged by size:
410 396 358 315 87 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 946 1219 1252 1617
Size: 946 273 33 365
Fragments arranged by size:
946 365 273 33
BsiHKAI G_wGCw'C
Cuts at: 0 59 365 1617
Size: 59 306 1252
Fragments arranged by size:
1252 306 59
Bsp1286I G_dGCh'C
Cuts at: 0 10 59 365 477 554 671 680 887
Size: 10 49 306 112 77 117 9 207
Cuts at: 887 913 1045 1529 1550 1578 1617
Size: 26 132 484 21 28 39
Fragments arranged by size:
484 306 207 132 117 112 77 49
39 28 26 21 10 9
BspEI T'CCGG_A
Cuts at: 0 113 1617
Size: 113 1504
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1274 1340 1617
Size: 1274 66 277
Fragments arranged by size:
1274 277 66
BsrBI CCG'CTC
Cuts at: 0 1022 1617
Size: 1022 595
BsrFI r'CCGG_y
Cuts at: 0 256 1280 1617
Size: 256 1024 337
Fragments arranged by size:
1024 337 256
BstYI r'GATC_y
Cuts at: 0 386 1169 1617
Size: 386 783 448
Fragments arranged by size:
783 448 386
Bsu36I CC'TnA_GG
Cuts at: 0 662 791 1034 1617
Size: 662 129 243 583
Fragments arranged by size:
662 583 243 129
BtgI C'CryG_G
Cuts at: 0 506 1065 1604 1617
Size: 506 559 539 13
Fragments arranged by size:
559 539 506 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 384 1617
Size: 384 1233
BtsI GCAGTG_nn'
Cuts at: 0 57 435 738 747 1215 1617
Size: 57 378 303 9 468 402
Fragments arranged by size:
468 402 378 303 57 9
ClaI AT'CG_AT
Cuts at: 0 929 1617
Size: 929 688
EaeI y'GGCC_r
Cuts at: 0 236 1464 1617
Size: 236 1228 153
Fragments arranged by size:
1228 236 153
EarI CTCTTCn'nnn_
Cuts at: 0 830 1617
Size: 830 787
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 218 557 1471 1617
Size: 218 339 914 146
Fragments arranged by size:
914 339 218 146
EcoICRI GAG'CTC
Cuts at: 0 57 1617
Size: 57 1560
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 403 508 536 1186 1384 1616 1617
Size: 349 54 105 28 650 198 232 1
Fragments arranged by size:
650 349 232 198 105 54 28 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 770 964 1617
Size: 770 194 653
Fragments arranged by size:
770 653 194
EcoO109I rG'GnC_Cy
Cuts at: 0 123 796 884 1097 1526 1546 1547 1617
Size: 123 673 88 213 429 20 1 70
Fragments arranged by size:
673 429 213 123 88 70 20 1
FspI TGC'GCA
Cuts at: 0 1012 1372 1617
Size: 1012 360 245
Fragments arranged by size:
1012 360 245
FspAI rTGC'GCAy
Cuts at: 0 1372 1617
Size: 1372 245
HaeII r_GCGC'y
Cuts at: 0 84 302 347 1200 1341 1386 1617
Size: 84 218 45 853 141 45 231
Fragments arranged by size:
853 231 218 141 84 45 45
HindIII A'AGCT_T
Cuts at: 0 181 617 1617
Size: 181 436 1000
Fragments arranged by size:
1000 436 181
KasI G'GCGC_C
Cuts at: 0 298 1382 1617
Size: 298 1084 235
Fragments arranged by size:
1084 298 235
MscI TGG'CCA
Cuts at: 0 1466 1617
Size: 1466 151
MslI CAynn'nnrTG
Cuts at: 0 50 98 1617
Size: 50 48 1519
Fragments arranged by size:
1519 50 48
MspA1I CmG'CkG
Cuts at: 0 87 358 891 1429 1444 1617
Size: 87 271 533 538 15 173
Fragments arranged by size:
538 533 271 173 87 15
NarI GG'CG_CC
Cuts at: 0 299 1383 1617
Size: 299 1084 234
Fragments arranged by size:
1084 299 234
NcoI C'CATG_G
Cuts at: 0 1065 1604 1617
Size: 1065 539 13
Fragments arranged by size:
1065 539 13
NheI G'CTAG_C
Cuts at: 0 153 1617
Size: 153 1464
NspI r_CATG'y
Cuts at: 0 354 1179 1263 1617
Size: 354 825 84 354
Fragments arranged by size:
825 354 354 84
PasI CC'CwG_GG
Cuts at: 0 1543 1617
Size: 1543 74
PflMI CCAn_nnn'nTGG
Cuts at: 0 486 862 1617
Size: 486 376 755
Fragments arranged by size:
755 486 376
PfoI T'CCnGG_A
Cuts at: 0 38 1617
Size: 38 1579
PpuMI rG'GwC_Cy
Cuts at: 0 796 1097 1617
Size: 796 301 520
Fragments arranged by size:
796 520 301
PspOMI G'GGCC_C
Cuts at: 0 883 1525 1546 1617
Size: 883 642 21 71
Fragments arranged by size:
883 642 71 21
PstI C_TGCA'G
Cuts at: 0 425 1337 1617
Size: 425 912 280
Fragments arranged by size:
912 425 280
PvuII CAG'CTG
Cuts at: 0 87 358 1617
Size: 87 271 1259
Fragments arranged by size:
1259 271 87
SacI G_AGCT'C
Cuts at: 0 59 1617
Size: 59 1558
SanDI GG'GwC_CC
Cuts at: 0 796 1617
Size: 796 821
SfcI C'TryA_G
Cuts at: 0 162 421 759 1333 1617
Size: 162 259 338 574 284
Fragments arranged by size:
574 338 284 259 162
SfoI GGC'GCC
Cuts at: 0 300 1384 1617
Size: 300 1084 233
Fragments arranged by size:
1084 300 233
SmlI C'TyrA_G
Cuts at: 0 1249 1617
Size: 1249 368
StuI AGG'CCT
Cuts at: 0 493 986 1617
Size: 493 493 631
Fragments arranged by size:
631 493 493
StyI C'CwwG_G
Cuts at: 0 632 1065 1319 1604 1617
Size: 632 433 254 285 13
Fragments arranged by size:
632 433 285 254 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 154 1593 1617
Size: 154 1439 24
Fragments arranged by size:
1439 154 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 376 408 1617
Size: 376 32 1209
Fragments arranged by size:
1209 376 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1467 1617
Size: 1467 150
XmnI GAAnn'nnTTC
Cuts at: 0 184 619 1617
Size: 184 435 998
Fragments arranged by size:
998 435 184
Enzymes that do cut:
AarI AcuI AleI AlwNI ApaI ApoI AvaI BanI
BanII BbeI BbsI BglI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI
BstYI Bsu36I BtgI BtgZI BtsI ClaI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI HaeII
HindIII KasI MscI MslI MspA1I NarI NcoI NheI
NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII
SacI SanDI SfcI SfoI SmlI StuI StyI TaqII
TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BsaAI BsaBI BsiEI BsiWI BsmI BsmBI
BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I CspCI DraI DraIII DrdI EagI EcoRI
EcoRV FalI FseI Hin4I HincII HpaI KpnI MfeI
MluI MmeI NaeI NdeI NgoMIV NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TatI Tth111I XbaI XhoI XmaI
ZraI