Restriction map for
pFN21AB7051
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BanII BbsI BcgI
BciVI BclI BfrI BglII BlnI
BpuAI BsaBI BsiEI BsiWI BsmBI
BsrFI BssHII BssSI BstAPI BstBI
BstEII BstPI Cfr10I CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HpaI
KpnI KspI MamI MfeI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I Van91I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AccI 97 GTATAC GT^[AC][GT]AC
AccII 868 CGCG CG^CG
AccIII 794 TCCGGA T^CCGGA
AcsI 1111 AAATTT [GA]^AATT[CT]
AcyI 255 GGCGCC G[GA]^CG[CT]C
Alw44I 1092 GTGCAC G^TGCAC
AocI 514 CCTGAGG CC^T[ACGT]AGG
ApaLI 1092 GTGCAC G^TGCAC
ApoI 1111 AAATTT [GA]^AATT[CT]
Asp700I 1161 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1092 GTGCAC G[AT]GC[AT]^C
AvaII 510 GGTCC G^G[AT]CC
BalI 8 TGGCCA TGG^CCA
BanI 255 GGCGCC G^G[CT][GA]CC
BbeI 255 GGCGCC GGCGC^C
BbrPI 1222 CACGTG CAC^GTG
BbvCI 259 CCTCAGC CCTCAGC(-5/-2)
BglI 1204 GCCAACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 46 GCTCAGC GC^T[ACGT]AGC
BmyI 1092 GTGCAC G[AGT]GC[ACT]^C
Bpu1102I 46 GCTCAGC GC^T[ACGT]AGC
BsaHI 255 GGCGCC G[GA]^CG[CT]C
BsaI 655 GAGACC GGTCTC(1/5)
BsaWI 794 TCCGGA [AT]^CCGG[AT]
BseAI 794 TCCGGA T^CCGGA
BsiHKAI 1092 GTGCAC G[AT]GC[AT]^C
BsmI 401 GCATTC GAATGC(1/-1)
Bsp1286I 1092 GTGCAC G[AGT]GC[ACT]^C
BspDI 985 ATCGAT AT^CGAT
BspEI 794 TCCGGA T^CCGGA
BspHI 728 TCATGA T^CATGA
BspLU11I 1142 ACATGT A^CATGT
BspMI 507 GCAGGT ACCTGC(4/8)
BsrBI 316 CCGCTC CCGCTC(-3/-3)
Bst1107I 97 GTATAC GTA^TAC
BstUI 868 CGCG CG^CG
BstZ17I 97 GTATAC GTA^TAC
Bsu36I 514 CCTGAGG CC^T[ACGT]AGG
CelII 46 GCTCAGC GC^T[ACGT]AGC
ClaI 985 ATCGAT AT^CGAT
DraII 509 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 1492 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 597 GACCTCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 514 CCTGAGG CC^T[ACGT]AGG
EcoNI 1044 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 509 AGGTCCC [GA]G^G[ACGT]CC[CT]
Hin1I 255 GGCGCC G[GA]^CG[CT]C
HindIII 1191 AAGCTT A^AGCTT
KasI 255 GGCGCC G^GCGCC
MluNI 8 TGGCCA TGG^CCA
MroI 794 TCCGGA T^CCGGA
MscI 8 TGGCCA TGG^CCA
MvnI 868 CGCG CG^CG
NarI 255 GGCGCC GG^CGCC
PmaCI 1222 CACGTG CAC^GTG
PmlI 1222 CACGTG CAC^GTG
PpuMI 509 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 728 TCATGA T^CATGA
ScaI 755 AGTACT AGT^ACT
SfoI 255 GGCGCC GGC^GCC
SmaI 646 CCCGGG CCC^GGG
StuI 518 AGGCCT AGG^CCT
XmaCI 646 CCCGGG C^CCGGG
XmaI 646 CCCGGG C^CCGGG
XmnI 1161 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 1142 ACATGT A^C[GA][CT]GT
1223 ACGTGT
AlwNI 449 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
1270 CAGACTCTG
BsaAI 806 TACGTG [CT]AC^GT[GA]
1222 CACGTG
BseRI 641 CTCCTC GAGGAG(10/8)
652 GAGGAG
Bsp1407I 239 TGTACA T^GTACA
1145 TGTACA
BsrDI 1026 CATTGC GCAATG(2/0)
890 GCAATG
BsrGI 239 TGTACA T^GTACA
1145 TGTACA
BstF5I 938 GGATG GGATG(2/0)
1419 GGATG
BstXI 768 CCACCTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1448 CCATTCTCCTGG
BstYI 471 AGATCC [GA]^GATC[CT]
821 AGATCC
CfoI 256 GCGC GCG^C
783 GCGC
EaeI 8 TGGCCA [CT]^GGCC[GA]
478 CGGCCA
EarI 732 GAAGAG CTCTTC(1/4)
1074 GAAGAG
FauI 315 CCCGC CCCGC(4/6)
1217 CCCGC
FokI 938 GGATG GGATG(9/13)
1419 GGATG
HaeII 255 GGCGCC [GA]GCGC^[CT]
782 GGCGCT
HhaI 256 GCGC GCG^C
783 GCGC
HinP1I 256 GCGC G^CGC
783 GCGC
Ksp632I 732 GAAGAG CTCTTC(1/4)
1074 GAAGAG
MflI 471 AGATCC [GA]^GATC[CT]
821 AGATCC
MspA1I 535 CAGCTG C[AC]G^C[GT]G
896 CAGCTG
NspI 1142 ACATGT [GA]CATG^[CT]
1316 ACATGC
PstI 592 CTGCAG CTGCA^G
958 CTGCAG
PvuII 535 CAGCTG CAG^CTG
896 CAGCTG
SspBI 239 TGTACA T^GTACA
1145 TGTACA
TfiI 561 GAATC G^A[AT]TC
973 GATTC
Tsp509I 139 AATT ^AATT
1112 AATT
XhoII 471 AGATCC [GA]^GATC[CT]
821 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
BbvI BcnI BfaI BpmI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI BsrI BssKI BstNI Cac8I
Cfr13I DdeI DpnII DsaI EcoRII
EcoT14I Fnu4HI HaeIII HapII HgaI
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI Tru9I
TseI Tsp45I TspRI TthHB8I