(Circular) (Six-base) MAPSORT of: pFN21AB7051.seq Check: 12 from: 1 to: 6247 pFN21AB7051 6247 bp With 182 enzymes: SgfI * August 20, 2012 11:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4057 278 Size: 53 83 186 936 2521 2468 Fragments arranged by size: 2521 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2103 3590 1937 Size: 166 1487 4594 Fragments arranged by size: 4594 1487 166 Acc65I G'GTAC_C Cuts at: 3568 3568 Size: 6247 AclI AA'CG_TT Cuts at: 1563 4374 4747 1563 Size: 2811 373 3063 Fragments arranged by size: 3063 2811 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2206 2436 2955 4310 5694 5962 1632 Size: 574 230 519 1355 1384 268 1917 Fragments arranged by size: 1917 1384 1355 574 519 268 230 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6050 AflIII A'CryG_T Cuts at: 1203 3147 3228 5146 6123 1203 Size: 1944 81 1918 977 1327 Fragments arranged by size: 1944 1918 1327 977 81 AgeI A'CCGG_T Cuts at: 5078 5078 Size: 6247 AhdI GACnn_n'nnGTC Cuts at: 4976 4976 Size: 6247 AleI CACnn'nnGTG Cuts at: 1166 2778 3263 1166 Size: 1612 485 4150 Fragments arranged by size: 4150 1612 485 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6247 AlwNI CAG_nnn'CTG Cuts at: 2459 3280 3990 5562 2459 Size: 821 710 1572 3144 Fragments arranged by size: 3144 1572 821 710 ApaLI G'TGCA_C Cuts at: 3097 4304 5460 3097 Size: 1207 1156 3884 Fragments arranged by size: 3884 1207 1156 ApoI r'AATT_y Cuts at: 1493 3116 3556 3782 3934 6043 1493 Size: 1623 440 226 152 2109 1697 Fragments arranged by size: 2109 1697 1623 440 226 152 AseI AT'TA_AT Cuts at: 160 1032 4801 160 Size: 872 3769 1606 Fragments arranged by size: 3769 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6247 AvaI C'yCGr_G Cuts at: 1870 1943 2468 2651 2909 3572 1870 Size: 73 525 183 258 663 4545 Fragments arranged by size: 4545 663 525 258 183 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2823 2856 2823 Size: 33 6214 BamHI G'GATC_C Cuts at: 3577 3944 3577 Size: 367 5880 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2260 3568 5023 618 Size: 325 217 627 473 1308 1455 1842 Fragments arranged by size: 1842 1455 1308 627 473 325 217 BanII G_rGCy'C Cuts at: 1391 3566 1391 Size: 2175 4072 BbeI G_GCGC'C Cuts at: 2264 2264 Size: 6247 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5276 BbvCI CC'TCA_GC Cuts at: 2265 2265 Size: 6247 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4438 4472 1903 Size: 34 2501 34 3678 Fragments arranged by size: 3678 2501 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4139 5349 4139 Size: 1210 5037 BclI T'GATC_A Cuts at: 1548 1548 Size: 6247 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3215 4858 136 Size: 107 122 71 631 605 1543 1643 1525 Fragments arranged by size: 1643 1543 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6242 6242 Size: 6247 BlpI GC'TnA_GC Cuts at: 2052 3664 2052 Size: 1612 4635 Bme1580I G_kGCm'C Cuts at: 1790 3101 4308 5464 1790 Size: 1311 1207 1156 2573 Fragments arranged by size: 2573 1311 1207 1156 BmgBI CAC'GTC Cuts at: 1318 2719 1318 Size: 1401 4846 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2259 3494 3978 Size: 416 53 83 399 24 842 1235 484 Cuts at: 3978 4936 442 Size: 958 1753 Fragments arranged by size: 1753 1235 958 842 484 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6247 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2479 2598 2807 3071 3157 4907 1239 Size: 1240 119 209 264 86 1750 2579 Fragments arranged by size: 2579 1750 1240 264 209 119 86 Bpu10I CC'TnA_GC Cuts at: 2265 2265 Size: 6247 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4372 5237 5535 4372 Size: 865 298 5084 Fragments arranged by size: 5084 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2654 4910 882 Size: 617 1155 2256 2219 Fragments arranged by size: 2256 2219 1155 617 BsaAI yAC'GTr Cuts at: 493 1204 2813 3229 4038 493 Size: 711 1609 416 809 2702 Fragments arranged by size: 2702 1609 809 711 416 BsaBI GATnn'nnATC Cuts at: 6241 6241 Size: 6247 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2261 4054 4436 5071 Size: 53 83 186 936 728 1793 382 635 Cuts at: 5071 275 Size: 1451 Fragments arranged by size: 1793 1451 936 728 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2799 4680 5078 5352 5499 5910 2799 Size: 1881 398 274 147 411 3136 Fragments arranged by size: 3136 1881 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6022 Fragments arranged by size: 6022 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2637 2672 1186 Size: 1451 35 4761 Fragments arranged by size: 4761 1451 35 BseYI C'CCAG_C Cuts at: 2538 5450 5946 2538 Size: 2912 496 2839 Fragments arranged by size: 2912 2839 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2065 2294 3199 3274 1163 Size: 573 329 229 905 75 4136 Fragments arranged by size: 4136 905 573 329 229 75 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3697 4458 5486 1540 Size: 275 186 1696 761 1028 2301 Fragments arranged by size: 2301 1696 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3101 4308 4393 5464 3101 Size: 1207 85 1071 3884 Fragments arranged by size: 3884 1207 1071 85 BsmI GAATG_Cn' Cuts at: 2406 3758 3851 2406 Size: 1352 93 4802 Fragments arranged by size: 4802 1352 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3101 3566 4308 4393 5464 1391 Size: 399 1311 465 742 85 1071 2174 Fragments arranged by size: 2174 1311 1071 742 465 399 85 BspEI T'CCGG_A Cuts at: 2799 5910 2799 Size: 3111 3136 BspHI T'CATG_A Cuts at: 2733 4136 5866 2733 Size: 1403 1730 3114 Fragments arranged by size: 3114 1730 1403 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2503 3602 844 Size: 1659 1099 3489 Fragments arranged by size: 3489 1659 1099 BsrBI CCG'CTC Cuts at: 1394 1823 2323 4134 1394 Size: 429 500 1811 3507 Fragments arranged by size: 3507 1811 500 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2902 3030 4742 4916 65 Size: 1183 1654 128 1712 174 1396 Fragments arranged by size: 1712 1654 1396 1183 174 128 BsrFI r'CCGG_y Cuts at: 1700 1810 4891 5078 1700 Size: 110 3081 187 2869 Fragments arranged by size: 3081 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2244 3150 96 Size: 2148 906 3193 Fragments arranged by size: 3193 2148 906 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6247 BssSI C'ACGA_G Cuts at: 4307 5319 4307 Size: 1012 5235 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6247 BstBI TT'CG_AA Cuts at: 5051 5051 Size: 6247 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6247 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2780 3460 2011 Size: 769 680 4798 Fragments arranged by size: 4798 769 680 BstYI r'GATC_y Cuts at: 1972 2476 2826 3577 3944 4329 4346 5798 5884 Size: 504 350 751 367 385 17 1452 86 Cuts at: 5884 5896 6242 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 751 504 385 367 350 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2104 2104 Size: 6247 Bsu36I CC'TnA_GG Cuts at: 2520 2520 Size: 6247 BtgI C'CryG_G Cuts at: 513 1065 2004 2020 2480 2922 3261 513 Size: 552 939 16 460 442 339 3499 Fragments arranged by size: 3499 939 552 460 442 339 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2112 487 Size: 683 56 886 4622 Fragments arranged by size: 4622 886 683 56 BtsI GCAGTG_nn' Cuts at: 3472 3763 4557 4577 3472 Size: 291 794 20 5142 Fragments arranged by size: 5142 794 291 20 ClaI AT'CG_AT Cuts at: 1554 2991 1554 Size: 1437 4810 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2428 2463 529 Size: 35 1864 35 4313 Fragments arranged by size: 4313 1864 35 35 DraI TTT'AAA Cuts at: 3551 3551 Size: 6247 DraIII CAC_nnn'GTG Cuts at: 3502 3502 Size: 6247 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2608 5254 809 Size: 507 1292 2646 1802 Fragments arranged by size: 2646 1802 1292 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2483 3694 Size: 54 1425 50 275 18 183 470 1211 Cuts at: 3694 4583 6212 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1211 889 470 275 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3694 1537 Size: 275 1882 4090 Fragments arranged by size: 4090 1882 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2732 3074 4177 1353 Size: 1379 342 1103 3423 Fragments arranged by size: 3423 1379 1103 342 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3100 4834 5208 5354 387 Size: 1064 138 1511 1734 374 146 1280 Fragments arranged by size: 1734 1511 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2206 2436 2479 2598 2807 2955 3071 Size: 393 574 230 43 119 209 148 116 Cuts at: 3071 3157 4310 4907 5694 5962 1239 Size: 86 1153 597 787 268 1524 Fragments arranged by size: 1524 1153 787 597 574 393 268 230 209 148 119 116 86 43 EcoNI CCTnn'n_nnAGG Cuts at: 3053 3053 Size: 6247 EcoO109I rG'GnC_Cy Cuts at: 2515 2515 Size: 6247 EcoRI G'AATT_C Cuts at: 3556 3934 3556 Size: 378 5869 FspI TGC'GCA Cuts at: 4753 4753 Size: 6247 HaeII r_GCGC'y Cuts at: 1678 2264 2791 5394 1678 Size: 586 527 2603 2531 Fragments arranged by size: 2603 2531 586 527 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2248 2280 2404 2436 2536 Size: 32 135 32 676 32 124 32 100 Cuts at: 2536 2568 3441 3473 4888 4920 4962 4994 1373 Size: 32 873 32 1415 32 42 32 2626 Fragments arranged by size: 2626 1415 873 676 135 124 100 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3591 3837 669 Size: 988 281 1653 246 3079 Fragments arranged by size: 3079 1653 988 281 246 HindIII A'AGCT_T Cuts at: 748 3196 748 Size: 2448 3799 HpaI GTT'AAC Cuts at: 3837 3837 Size: 6247 KasI G'GCGC_C Cuts at: 2260 2260 Size: 6247 KpnI G_GTAC'C Cuts at: 3572 3572 Size: 6247 MfeI C'AATT_G Cuts at: 3846 3846 Size: 6247 MluI A'CGCG_T Cuts at: 6123 6123 Size: 6247 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2988 5361 5545 1305 Size: 1683 2373 184 2007 Fragments arranged by size: 2373 2007 1683 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2015 10 Size: 54 1425 343 183 4242 Fragments arranged by size: 4242 1425 343 183 54 MslI CAynn'nnrTG Cuts at: 518 1166 2778 2927 3263 3326 4205 4564 4723 Size: 648 1612 149 336 63 879 359 159 Cuts at: 4723 518 Size: 2042 Fragments arranged by size: 2042 1612 879 648 359 336 159 149 63 MspA1I CmG'CkG Cuts at: 1606 2542 2903 3663 4340 5488 5733 1606 Size: 936 361 760 677 1148 245 2120 Fragments arranged by size: 2120 1148 936 760 677 361 245 NarI GG'CG_CC Cuts at: 2261 2261 Size: 6247 NcoI C'CATG_G Cuts at: 513 1065 2004 2020 2922 3261 513 Size: 552 939 16 902 339 3499 Fragments arranged by size: 3499 939 902 552 339 16 NdeI CA'TA_TG Cuts at: 387 387 Size: 6247 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6247 NotI GC'GGCC_GC Cuts at: 3694 3694 Size: 6247 NspI r_CATG'y Cuts at: 1132 1229 3151 3325 3605 5150 1132 Size: 97 1922 174 280 1545 2229 Fragments arranged by size: 2229 1922 1545 280 174 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6247 PciI A'CATG_T Cuts at: 3147 5146 3147 Size: 1999 4248 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6247 PfoI T'CCnGG_A Cuts at: 2495 2495 Size: 6247 PmeI GTTT'AAAC Cuts at: 3551 3551 Size: 6247 PmlI CAC'GTG Cuts at: 3229 3229 Size: 6247 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4316 4348 5829 5861 4316 Size: 32 1481 32 4702 Fragments arranged by size: 4702 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2515 2515 Size: 6247 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6247 PsiI TTA'TAA Cuts at: 3817 3817 Size: 6247 PstI C_TGCA'G Cuts at: 830 2601 2967 3599 830 Size: 1771 366 632 3478 Fragments arranged by size: 3478 1771 632 366 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6247 PvuII CAG'CTG Cuts at: 2542 2903 2542 Size: 361 5886 SalI G'TCGA_C Cuts at: 1936 3589 1936 Size: 1653 4594 SbfI CC_TGCA'GG Cuts at: 3599 3599 Size: 6247 ScaI AGT'ACT Cuts at: 2762 4495 6207 2762 Size: 1733 1712 2802 Fragments arranged by size: 2802 1733 1712 SfcI C'TryA_G Cuts at: 826 1045 2597 2890 2963 3595 4730 5411 5602 Size: 219 1552 293 73 632 1135 681 191 Cuts at: 5602 826 Size: 1471 Fragments arranged by size: 1552 1471 1135 681 632 293 219 191 73 SfoI GGC'GCC Cuts at: 2262 2262 Size: 6247 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6247 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6247 SmaI CCC'GGG Cuts at: 1872 2653 3574 1872 Size: 781 921 4545 Fragments arranged by size: 4545 921 781 SmlI C'TyrA_G Cuts at: 820 1017 1943 4351 5252 5514 820 Size: 197 926 2408 901 262 1553 Fragments arranged by size: 2408 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4038 493 Size: 3545 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6247 SphI G_CATG'C Cuts at: 1132 3605 1132 Size: 2473 3774 SspI AAT'ATT Cuts at: 5 52 4171 5 Size: 47 4119 2081 Fragments arranged by size: 4119 2081 47 StuI AGG'CCT Cuts at: 1517 2525 1517 Size: 1008 5239 StyI C'CwwG_G Cuts at: 513 1065 2004 2020 2922 3261 3686 513 Size: 552 939 16 902 339 425 3074 Fragments arranged by size: 3074 939 902 552 425 339 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3513 4274 4291 4444 4629 5074 325 Size: 1255 1933 761 17 153 185 445 1498 Fragments arranged by size: 1933 1498 1255 761 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2244 2760 3150 Size: 274 80 33 51 1443 267 516 390 Cuts at: 3150 4493 6205 96 Size: 1343 1712 138 Fragments arranged by size: 1712 1443 1343 516 390 274 267 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2620 2652 2935 2967 6207 6239 2620 Size: 32 283 32 3240 32 2628 Fragments arranged by size: 3240 2628 283 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6247 XbaI T'CTAG_A Cuts at: 3583 3583 Size: 6247 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6247 XmaI C'CCGG_G Cuts at: 1870 2651 3572 1870 Size: 781 921 4545 Fragments arranged by size: 4545 921 781 XmnI GAAnn'nnTTC Cuts at: 3170 4376 3170 Size: 1206 5041 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4055 276 Size: 53 83 186 936 2521 2468 Fragments arranged by size: 2521 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BfrBI BplI BsiWI BsmBI EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI