Restriction map for
pFN21AB7051
(Linear) (Six-base) MAPSORT of: KIBB7051.seq Check: 6027 from: 1 to: 1542
KIBB7051.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AccI GT'mk_AC
Cuts at: 0 98 1542
Size: 98 1444
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 431 950 1542
Size: 201 230 519 592
Fragments arranged by size:
592 519 230 201
AflIII A'CryG_T
Cuts at: 0 1142 1223 1542
Size: 1142 81 319
Fragments arranged by size:
1142 319 81
AleI CACnn'nnGTG
Cuts at: 0 773 1258 1542
Size: 773 485 284
Fragments arranged by size:
773 485 284
AlwNI CAG_nnn'CTG
Cuts at: 0 454 1275 1542
Size: 454 821 267
Fragments arranged by size:
821 454 267
ApaLI G'TGCA_C
Cuts at: 0 1092 1542
Size: 1092 450
ApoI r'AATT_y
Cuts at: 0 1111 1542
Size: 1111 431
AvaI C'yCGr_G
Cuts at: 0 463 646 904 1542
Size: 463 183 258 638
Fragments arranged by size:
638 463 258 183
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 818 851 1542
Size: 818 33 691
Fragments arranged by size:
818 691 33
BanI G'GyrC_C
Cuts at: 0 255 1542
Size: 255 1287
BbeI G_GCGC'C
Cuts at: 0 259 1542
Size: 259 1283
BbvCI CC'TCA_GC
Cuts at: 0 260 1542
Size: 260 1282
BglI GCCn_nnn'nGGC
Cuts at: 0 1210 1542
Size: 1210 332
BlpI GC'TnA_GC
Cuts at: 0 47 1542
Size: 47 1495
Bme1580I G_kGCm'C
Cuts at: 0 1096 1542
Size: 1096 446
BmgBI CAC'GTC
Cuts at: 0 714 1542
Size: 714 828
BmrI ACTGGGnnnn_n'
Cuts at: 0 254 1489 1542
Size: 254 1235 53
Fragments arranged by size:
1235 254 53
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 474 593 802 1066 1152 1542
Size: 474 119 209 264 86 390
Fragments arranged by size:
474 390 264 209 119 86
Bpu10I CC'TnA_GC
Cuts at: 0 260 1542
Size: 260 1282
BsaI GGTCTCn'nnnn_
Cuts at: 0 649 1542
Size: 649 893
BsaAI yAC'GTr
Cuts at: 0 808 1224 1542
Size: 808 416 318
Fragments arranged by size:
808 416 318
BsaHI Gr'CG_yC
Cuts at: 0 256 1542
Size: 256 1286
BsaWI w'CCGG_w
Cuts at: 0 794 1542
Size: 794 748
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 632 667 1542
Size: 632 35 875
Fragments arranged by size:
875 632 35
BseYI C'CCAG_C
Cuts at: 0 533 1542
Size: 533 1009
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 289 1194 1269 1542
Size: 60 229 905 75 273
Fragments arranged by size:
905 273 229 75 60
BsiHKAI G_wGCw'C
Cuts at: 0 1096 1542
Size: 1096 446
BsmI GAATG_Cn'
Cuts at: 0 401 1542
Size: 401 1141
Bsp1286I G_dGCh'C
Cuts at: 0 1096 1542
Size: 1096 446
BspEI T'CCGG_A
Cuts at: 0 794 1542
Size: 794 748
BspHI T'CATG_A
Cuts at: 0 728 1542
Size: 728 814
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 498 1542
Size: 498 1044
BsrBI CCG'CTC
Cuts at: 0 318 1542
Size: 318 1224
BsrDI GCAATG_nn'
Cuts at: 0 897 1025 1542
Size: 897 128 517
Fragments arranged by size:
897 517 128
BsrGI T'GTAC_A
Cuts at: 0 239 1145 1542
Size: 239 906 397
Fragments arranged by size:
906 397 239
BstXI CCAn_nnnn'nTGG
Cuts at: 0 775 1455 1542
Size: 775 680 87
Fragments arranged by size:
775 680 87
BstYI r'GATC_y
Cuts at: 0 471 821 1542
Size: 471 350 721
Fragments arranged by size:
721 471 350
BstZ17I GTA'TAC
Cuts at: 0 99 1542
Size: 99 1443
Bsu36I CC'TnA_GG
Cuts at: 0 515 1542
Size: 515 1027
BtgI C'CryG_G
Cuts at: 0 15 475 917 1256 1542
Size: 15 460 442 339 286
Fragments arranged by size:
460 442 339 286 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 107 1542
Size: 107 1435
BtsI GCAGTG_nn'
Cuts at: 0 1467 1542
Size: 1467 75
ClaI AT'CG_AT
Cuts at: 0 986 1542
Size: 986 556
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 423 458 1542
Size: 423 35 1084
Fragments arranged by size:
1084 423 35
DraIII CAC_nnn'GTG
Cuts at: 0 1497 1542
Size: 1497 45
DrdI GACnn_nn'nnGTC
Cuts at: 0 603 1542
Size: 603 939
EaeI y'GGCC_r
Cuts at: 0 8 478 1542
Size: 8 470 1064
Fragments arranged by size:
1064 470 8
EarI CTCTTCn'nnn_
Cuts at: 0 727 1069 1542
Size: 727 342 473
Fragments arranged by size:
727 473 342
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1095 1542
Size: 1095 447
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 431 474 593 802 950 1066 1152
Size: 201 230 43 119 209 148 116 86
Cuts at: 1152 1542
Size: 390
Fragments arranged by size:
390 230 209 201 148 119 116 86
43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1048 1542
Size: 1048 494
EcoO109I rG'GnC_Cy
Cuts at: 0 510 1542
Size: 510 1032
HaeII r_GCGC'y
Cuts at: 0 259 786 1542
Size: 259 527 756
Fragments arranged by size:
756 527 259
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 243 275 399 431 531 563 1436 1468
Size: 243 32 124 32 100 32 873 32
Cuts at: 1468 1542
Size: 74
Fragments arranged by size:
873 243 124 100 74 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 1191 1542
Size: 1191 351
KasI G'GCGC_C
Cuts at: 0 255 1542
Size: 255 1287
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 983 1542
Size: 983 559
MscI TGG'CCA
Cuts at: 0 10 1542
Size: 10 1532
MslI CAynn'nnrTG
Cuts at: 0 773 922 1258 1321 1542
Size: 773 149 336 63 221
Fragments arranged by size:
773 336 221 149 63
MspA1I CmG'CkG
Cuts at: 0 537 898 1542
Size: 537 361 644
Fragments arranged by size:
644 537 361
NarI GG'CG_CC
Cuts at: 0 256 1542
Size: 256 1286
NcoI C'CATG_G
Cuts at: 0 15 917 1256 1542
Size: 15 902 339 286
Fragments arranged by size:
902 339 286 15
NspI r_CATG'y
Cuts at: 0 1146 1320 1542
Size: 1146 174 222
Fragments arranged by size:
1146 222 174
PciI A'CATG_T
Cuts at: 0 1142 1542
Size: 1142 400
PfoI T'CCnGG_A
Cuts at: 0 490 1542
Size: 490 1052
PmlI CAC'GTG
Cuts at: 0 1224 1542
Size: 1224 318
PpuMI rG'GwC_Cy
Cuts at: 0 510 1542
Size: 510 1032
PstI C_TGCA'G
Cuts at: 0 596 962 1542
Size: 596 366 580
Fragments arranged by size:
596 580 366
PvuII CAG'CTG
Cuts at: 0 537 898 1542
Size: 537 361 644
Fragments arranged by size:
644 537 361
ScaI AGT'ACT
Cuts at: 0 757 1542
Size: 757 785
SfcI C'TryA_G
Cuts at: 0 592 885 958 1542
Size: 592 293 73 584
Fragments arranged by size:
592 584 293 73
SfoI GGC'GCC
Cuts at: 0 257 1542
Size: 257 1285
SmaI CCC'GGG
Cuts at: 0 648 1542
Size: 648 894
StuI AGG'CCT
Cuts at: 0 520 1542
Size: 520 1022
StyI C'CwwG_G
Cuts at: 0 15 917 1256 1542
Size: 15 902 339 286
Fragments arranged by size:
902 339 286 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1508 1542
Size: 1508 34
TatI w'GTAC_w
Cuts at: 0 239 755 1145 1542
Size: 239 516 390 397
Fragments arranged by size:
516 397 390 239
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 615 647 930 962 1542
Size: 615 32 283 32 580
Fragments arranged by size:
615 580 283 32 32
XmaI C'CCGG_G
Cuts at: 0 646 1542
Size: 646 896
XmnI GAAnn'nnTTC
Cuts at: 0 1165 1542
Size: 1165 377
Enzymes that do cut:
AccI AcuI AflIII AleI AlwNI ApaLI ApoI AvaI
BaeI BanI BbeI BbvCI BglI BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrGI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EarI
EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII KasI
MmeI MscI MslI MspA1I NarI NcoI NspI PciI
PfoI PmlI PpuMI PstI PvuII ScaI SfcI SfoI
SmaI StuI StyI TaqII TatI TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI AscI AseI AsiSI AvrII BamHI
BanII BbsI BcgI BciVI BclI BfrBI BglII BmtI
BplI BpuEI BsaBI BsaXI BsiEI BsiWI BsmBI BsrFI
BssHII BssSI BstAPI BstBI BstEII DraI EagI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI
KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XcmI XhoI ZraI