(Circular) (Six-base) MAPSORT of: pFN21AB7050.seq Check: 4900 from: 1 to: 6217 pFN21AB7050 6217 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4027 278 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3560 1937 Size: 1623 4594 Acc65I G'GTAC_C Cuts at: 2113 3538 2113 Size: 1425 4792 AclI AA'CG_TT Cuts at: 1563 4344 4717 1563 Size: 2781 373 3063 Fragments arranged by size: 3063 2781 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3478 4280 5664 5932 1632 Size: 1846 802 1384 268 1917 Fragments arranged by size: 1917 1846 1384 802 268 AfeI AGC'GCT Cuts at: 2328 2345 2328 Size: 17 6200 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6020 AflIII A'CryG_T Cuts at: 1203 5116 6093 1203 Size: 3913 977 1327 Fragments arranged by size: 3913 1327 977 AgeI A'CCGG_T Cuts at: 5048 5048 Size: 6217 AhdI GACnn_n'nnGTC Cuts at: 4946 4946 Size: 6217 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6217 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6217 AlwNI CAG_nnn'CTG Cuts at: 3960 5532 3960 Size: 1572 4645 ApaLI G'TGCA_C Cuts at: 2380 4274 5430 2380 Size: 1894 1156 3167 Fragments arranged by size: 3167 1894 1156 ApoI r'AATT_y Cuts at: 1493 3526 3752 3904 6013 1493 Size: 2033 226 152 2109 1697 Fragments arranged by size: 2109 2033 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4771 160 Size: 872 3739 1606 Fragments arranged by size: 3739 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 AvaI C'yCGr_G Cuts at: 1870 1943 2209 2237 2463 2488 3542 1870 Size: 73 266 28 226 25 1054 4545 Fragments arranged by size: 4545 1054 266 226 73 28 25 AvrII C'CTAG_G Cuts at: 2746 2746 Size: 6217 BamHI G'GATC_C Cuts at: 3547 3914 3547 Size: 367 5850 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2113 2594 3538 4993 618 Size: 325 217 627 326 481 944 1455 1842 Fragments arranged by size: 1842 1455 944 627 481 326 325 217 BanII G_rGCy'C Cuts at: 1391 2496 3536 1391 Size: 1105 1040 4072 Fragments arranged by size: 4072 1105 1040 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5246 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2371 2405 4408 4442 1903 Size: 34 434 34 2003 34 3678 Fragments arranged by size: 3678 2003 434 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2843 4109 5319 2843 Size: 1266 1210 3741 Fragments arranged by size: 3741 1266 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6217 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2423 4828 136 Size: 107 122 71 631 605 751 2405 1525 Fragments arranged by size: 2405 1525 751 631 605 122 107 71 BglII A'GATC_T Cuts at: 6212 6212 Size: 6217 BlpI GC'TnA_GC Cuts at: 3634 3634 Size: 6217 Bme1580I G_kGCm'C Cuts at: 1790 2384 4278 5434 1790 Size: 594 1894 1156 2573 Fragments arranged by size: 2573 1894 1156 594 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6217 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2600 3948 4906 Size: 416 53 83 399 24 1183 1348 958 Cuts at: 4906 442 Size: 1753 Fragments arranged by size: 1753 1348 1183 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6217 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2264 2871 4877 1239 Size: 1025 607 2006 2579 Fragments arranged by size: 2579 2006 1025 607 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2315 2620 2959 4342 5207 5505 2315 Size: 305 339 1383 865 298 3027 Fragments arranged by size: 3027 1383 865 339 305 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2025 4880 882 Size: 617 526 2855 2219 Fragments arranged by size: 2855 2219 617 526 BsaAI yAC'GTr Cuts at: 493 1204 4008 493 Size: 711 2804 2702 Fragments arranged by size: 2804 2702 711 BsaBI GATnn'nnATC Cuts at: 6211 6211 Size: 6217 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2285 4024 4406 5041 Size: 53 83 186 936 752 1739 382 635 Cuts at: 5041 275 Size: 1451 Fragments arranged by size: 1739 1451 936 752 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3514 4650 5048 5322 5469 5880 3514 Size: 1136 398 274 147 411 3851 Fragments arranged by size: 3851 1136 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2463 2493 1178 Size: 30 165 30 1060 30 4902 Fragments arranged by size: 4902 1060 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2465 2832 3225 1186 Size: 1279 367 393 4178 Fragments arranged by size: 4178 1279 393 367 BseYI C'CCAG_C Cuts at: 2137 2156 2584 5420 5916 2137 Size: 19 428 2836 496 2438 Fragments arranged by size: 2836 2438 496 428 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5644 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2050 2153 2181 2187 3667 4428 Size: 275 186 49 103 28 6 1480 761 Cuts at: 4428 5456 1540 Size: 1028 2301 Fragments arranged by size: 2301 1480 1028 761 275 186 103 49 28 6 BsiHKAI G_wGCw'C Cuts at: 2384 2489 4278 4363 5434 2384 Size: 105 1789 85 1071 3167 Fragments arranged by size: 3167 1789 1071 105 85 BsmI GAATG_Cn' Cuts at: 3045 3728 3821 3045 Size: 683 93 5441 Fragments arranged by size: 5441 683 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2384 2489 2496 3536 4278 4363 5434 Size: 399 594 105 7 1040 742 85 1071 Cuts at: 5434 1391 Size: 2174 Fragments arranged by size: 2174 1071 1040 742 594 399 105 85 7 BspEI T'CCGG_A Cuts at: 5880 5880 Size: 6217 BspHI T'CATG_A Cuts at: 4106 5836 4106 Size: 1730 4487 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2119 3572 844 Size: 1275 1453 3489 Fragments arranged by size: 3489 1453 1275 BsrBI CCG'CTC Cuts at: 1394 1823 2011 2250 4104 1394 Size: 429 188 239 1854 3507 Fragments arranged by size: 3507 1854 429 239 188 BsrDI GCAATG_nn' Cuts at: 65 1248 4712 4886 65 Size: 1183 3464 174 1396 Fragments arranged by size: 3464 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2110 2230 3033 4861 5048 1700 Size: 110 300 120 803 1828 187 2869 Fragments arranged by size: 2869 1828 803 300 187 120 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6217 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6217 BssSI C'ACGA_G Cuts at: 4277 5289 4277 Size: 1012 5205 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6217 BstBI TT'CG_AA Cuts at: 2634 5021 2634 Size: 2387 3830 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6217 BstYI r'GATC_y Cuts at: 1972 2986 3547 3914 4299 4316 5768 5854 5866 Size: 1014 561 367 385 17 1452 86 12 Cuts at: 5866 6212 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1014 561 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2658 2658 Size: 6217 BtgI C'CryG_G Cuts at: 513 1065 2004 2181 2279 2311 2556 513 Size: 552 939 177 98 32 245 4174 Fragments arranged by size: 4174 939 552 245 177 98 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5478 Fragments arranged by size: 5478 683 56 BtsI GCAGTG_nn' Cuts at: 3733 4527 4547 3733 Size: 794 20 5403 Fragments arranged by size: 5403 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6217 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6182 DraI TTT'AAA Cuts at: 3096 3521 3096 Size: 425 5792 DraIII CAC_nnn'GTG Cuts at: 2021 2021 Size: 6217 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5224 809 Size: 507 3908 1802 Fragments arranged by size: 3908 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2047 2150 2178 Size: 54 1425 50 275 18 217 103 28 Cuts at: 2178 2184 2412 3404 3664 4553 6182 8 Size: 6 228 992 260 889 1629 43 Fragments arranged by size: 1629 1425 992 889 275 260 228 217 103 54 50 43 28 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2047 2150 2178 2184 3664 1537 Size: 275 235 103 28 6 1480 4090 Fragments arranged by size: 4090 1480 275 235 103 28 6 EarI CTCTTCn'nnn_ Cuts at: 1353 2865 3243 3334 4147 1353 Size: 1512 378 91 813 3423 Fragments arranged by size: 3423 1512 813 378 91 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2079 4804 5178 5324 387 Size: 1064 138 490 2725 374 146 1280 Fragments arranged by size: 2725 1280 1064 490 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2264 2871 3478 4280 4877 5664 5932 Size: 393 632 607 607 802 597 787 268 Cuts at: 5932 1239 Size: 1524 Fragments arranged by size: 1524 802 787 632 607 607 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3297 3297 Size: 6217 EcoO109I rG'GnC_Cy Cuts at: 2883 2883 Size: 6217 EcoRI G'AATT_C Cuts at: 3526 3904 3526 Size: 378 5839 EcoRV GAT'ATC Cuts at: 2998 2998 Size: 6217 FspI TGC'GCA Cuts at: 2432 4723 2432 Size: 2291 3926 HaeII r_GCGC'y Cuts at: 1678 2330 2347 2401 2429 5364 1678 Size: 652 17 54 28 2935 2531 Fragments arranged by size: 2935 2531 652 54 28 17 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2891 2923 3288 3320 4858 Size: 32 135 32 1319 32 365 32 1538 Cuts at: 4858 4890 4932 4964 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1538 1319 365 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3561 3807 669 Size: 988 281 1623 246 3079 Fragments arranged by size: 3079 1623 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6217 HpaI GTT'AAC Cuts at: 3807 3807 Size: 6217 KpnI G_GTAC'C Cuts at: 2117 3542 2117 Size: 1425 4792 MfeI C'AATT_G Cuts at: 3816 3816 Size: 6217 MluI A'CGCG_T Cuts at: 6093 6093 Size: 6217 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2841 2987 5331 5515 1305 Size: 1536 146 2344 184 2007 Fragments arranged by size: 2344 2007 1536 184 146 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4395 Fragments arranged by size: 4395 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4175 4534 4693 518 Size: 648 3009 359 159 2042 Fragments arranged by size: 3009 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2177 2183 2198 2281 2301 2355 3633 4310 Size: 571 6 15 83 20 54 1278 677 Cuts at: 4310 5458 5703 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1278 1148 677 571 245 83 54 20 15 6 NaeI GCC'GGC Cuts at: 2232 3035 2232 Size: 803 5414 NcoI C'CATG_G Cuts at: 513 1065 2004 2311 513 Size: 552 939 307 4419 Fragments arranged by size: 4419 939 552 307 NdeI CA'TA_TG Cuts at: 387 387 Size: 6217 NgoMIV G'CCGG_C Cuts at: 2230 3033 2230 Size: 803 5414 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6217 NotI GC'GGCC_GC Cuts at: 2150 2178 2184 3664 2150 Size: 28 6 1480 4703 Fragments arranged by size: 4703 1480 28 6 NspI r_CATG'y Cuts at: 1132 1229 3575 5120 1132 Size: 97 2346 1545 2229 Fragments arranged by size: 2346 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2066 1348 Size: 718 5499 PciI A'CATG_T Cuts at: 5116 5116 Size: 6217 PflMI CCAn_nnn'nTGG Cuts at: 1108 2317 1108 Size: 1209 5008 PfoI T'CCnGG_A Cuts at: 2848 2848 Size: 6217 PmeI GTTT'AAAC Cuts at: 3521 3521 Size: 6217 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4286 4318 5799 5831 4286 Size: 32 1481 32 4672 Fragments arranged by size: 4672 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2883 2883 Size: 6217 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6217 PsiI TTA'TAA Cuts at: 3787 3787 Size: 6217 PstI C_TGCA'G Cuts at: 830 3337 3569 830 Size: 2507 232 3478 Fragments arranged by size: 3478 2507 232 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6217 SacII CC_GC'GG Cuts at: 2184 2282 2184 Size: 98 6119 SalI G'TCGA_C Cuts at: 1936 3559 1936 Size: 1623 4594 SbfI CC_TGCA'GG Cuts at: 3569 3569 Size: 6217 ScaI AGT'ACT Cuts at: 4465 6177 4465 Size: 1712 4505 SfcI C'TryA_G Cuts at: 826 1045 2471 3333 3565 4700 5381 5572 826 Size: 219 1426 862 232 1135 681 191 1471 Fragments arranged by size: 1471 1426 1135 862 681 232 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6217 SmaI CCC'GGG Cuts at: 1872 2239 2465 3544 1872 Size: 367 226 1079 4545 Fragments arranged by size: 4545 1079 367 226 SmlI C'TyrA_G Cuts at: 820 1017 1943 2330 2599 2974 4321 5222 5484 Size: 197 926 387 269 375 1347 901 262 Cuts at: 5484 820 Size: 1553 Fragments arranged by size: 1553 1347 926 901 387 375 269 262 197 SnaBI TAC'GTA Cuts at: 493 4008 493 Size: 3515 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6217 SphI G_CATG'C Cuts at: 1132 3575 1132 Size: 2443 3774 SspI AAT'ATT Cuts at: 5 52 4141 5 Size: 47 4089 2081 Fragments arranged by size: 4089 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6217 StyI C'CwwG_G Cuts at: 513 1065 2004 2311 2746 2949 3656 513 Size: 552 939 307 435 203 707 3074 Fragments arranged by size: 3074 939 707 552 435 307 203 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2049 2582 4244 4261 4414 4599 5044 Size: 1255 469 533 1662 17 153 185 445 Cuts at: 5044 325 Size: 1498 Fragments arranged by size: 1662 1498 1255 533 469 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3489 4463 6175 Size: 274 80 33 51 1443 1512 974 1712 Cuts at: 6175 96 Size: 138 Fragments arranged by size: 1712 1512 1443 974 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2354 2386 6177 6209 2354 Size: 32 3791 32 2362 Fragments arranged by size: 3791 2362 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2968 1316 Size: 1652 4565 XbaI T'CTAG_A Cuts at: 3553 3553 Size: 6217 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6217 XmaI C'CCGG_G Cuts at: 1870 2237 2463 3542 1870 Size: 367 226 1079 4545 Fragments arranged by size: 4545 1079 367 226 XmnI GAAnn'nnTTC Cuts at: 3045 4346 3045 Size: 1301 4916 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4025 276 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI