(Circular) (Six-base) MAPSORT of: pFN21AB7049.seq Check: 2332 from: 1 to: 6181 pFN21AB7049 6181 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3991 278 Size: 53 83 186 936 2455 2468 Fragments arranged by size: 2468 2455 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3524 1937 Size: 1587 4594 Acc65I G'GTAC_C Cuts at: 3502 3502 Size: 6181 AclI AA'CG_TT Cuts at: 1563 4308 4681 1563 Size: 2745 373 3063 Fragments arranged by size: 3063 2745 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2072 3351 3420 3450 4244 5628 5896 1632 Size: 440 1279 69 30 794 1384 268 1917 Fragments arranged by size: 1917 1384 1279 794 440 268 69 30 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5984 AflIII A'CryG_T Cuts at: 1203 5080 6057 1203 Size: 3877 977 1327 Fragments arranged by size: 3877 1327 977 AgeI A'CCGG_T Cuts at: 5012 5012 Size: 6181 AhdI GACnn_n'nnGTC Cuts at: 2815 3465 4910 2815 Size: 650 1445 4086 Fragments arranged by size: 4086 1445 650 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6181 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2838 1184 Size: 1654 4527 AlwNI CAG_nnn'CTG Cuts at: 3924 5496 3924 Size: 1572 4609 ApaLI G'TGCA_C Cuts at: 4238 5394 4238 Size: 1156 5025 ApoI r'AATT_y Cuts at: 1493 3254 3490 3716 3868 5977 1493 Size: 1761 236 226 152 2109 1697 Fragments arranged by size: 2109 1761 1697 236 226 152 AseI AT'TA_AT Cuts at: 160 1032 4735 160 Size: 872 3703 1606 Fragments arranged by size: 3703 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 AvaI C'yCGr_G Cuts at: 1870 1943 3282 3506 1870 Size: 73 1339 224 4545 Fragments arranged by size: 4545 1339 224 73 BamHI G'GATC_C Cuts at: 2231 3418 3511 3878 2231 Size: 1187 93 367 4534 Fragments arranged by size: 4534 1187 367 93 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2020 3502 4957 618 Size: 325 217 627 233 1482 1455 1842 Fragments arranged by size: 1842 1482 1455 627 325 233 217 BanII G_rGCy'C Cuts at: 1391 3018 3083 3500 1391 Size: 1627 65 417 4072 Fragments arranged by size: 4072 1627 417 65 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5210 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4372 4406 1903 Size: 34 50 34 2351 34 3678 Fragments arranged by size: 3678 2351 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4073 5283 4073 Size: 1210 4971 BclI T'GATC_A Cuts at: 1548 1548 Size: 6181 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4792 136 Size: 107 122 71 631 605 3120 1525 Fragments arranged by size: 3120 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3395 6176 3395 Size: 2781 3400 BlpI GC'TnA_GC Cuts at: 3598 3598 Size: 6181 Bme1580I G_kGCm'C Cuts at: 1790 2025 2513 4242 5398 1790 Size: 235 488 1729 1156 2573 Fragments arranged by size: 2573 1729 1156 488 235 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6181 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2036 2726 2955 Size: 416 53 83 399 24 619 690 229 Cuts at: 2955 3163 3912 4870 442 Size: 208 749 958 1753 Fragments arranged by size: 1753 958 749 690 619 416 399 229 208 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6181 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4841 1239 Size: 3602 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2627 4306 5171 5469 2627 Size: 1679 865 298 3339 Fragments arranged by size: 3339 1679 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3473 4844 882 Size: 617 1974 1371 2219 Fragments arranged by size: 2219 1974 1371 617 BsaAI yAC'GTr Cuts at: 493 1204 2465 3972 493 Size: 711 1261 1507 2702 Fragments arranged by size: 2702 1507 1261 711 BsaBI GATnn'nnATC Cuts at: 2604 6175 2604 Size: 3571 2610 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3988 4370 5005 275 Size: 53 83 186 936 2455 382 635 1451 Fragments arranged by size: 2455 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3464 4614 5012 5286 5433 5844 3464 Size: 1150 398 274 147 411 3801 Fragments arranged by size: 3801 1150 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2768 2798 1178 Size: 30 165 30 1365 30 4561 Fragments arranged by size: 4561 1365 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3461 1186 Size: 2275 3906 BseYI C'CCAG_C Cuts at: 3443 3477 5384 5880 3443 Size: 34 1907 496 3744 Fragments arranged by size: 3744 1907 496 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2499 1163 Size: 573 763 4845 Fragments arranged by size: 4845 763 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3631 4392 5420 1540 Size: 275 186 1630 761 1028 2301 Fragments arranged by size: 2301 1630 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3018 4242 4327 5398 3018 Size: 1224 85 1071 3801 Fragments arranged by size: 3801 1224 1071 85 BsmI GAATG_Cn' Cuts at: 3692 3785 3692 Size: 93 6088 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2513 3018 3083 3500 4242 4327 Size: 399 235 488 505 65 417 742 85 Cuts at: 4327 5398 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 505 488 417 399 235 85 65 BspEI T'CCGG_A Cuts at: 5844 5844 Size: 6181 BspHI T'CATG_A Cuts at: 2596 3010 4070 5800 2596 Size: 414 1060 1730 2977 Fragments arranged by size: 2977 1730 1060 414 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3536 844 Size: 2692 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4068 1394 Size: 429 2245 3507 Fragments arranged by size: 3507 2245 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3240 4676 4850 65 Size: 1183 1992 1436 174 1396 Fragments arranged by size: 1992 1436 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4825 5012 1700 Size: 110 3015 187 2869 Fragments arranged by size: 3015 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2503 2566 96 Size: 2407 63 3711 Fragments arranged by size: 3711 2407 63 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6181 BssSI C'ACGA_G Cuts at: 4241 5253 4241 Size: 1012 5169 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6181 BstBI TT'CG_AA Cuts at: 4985 4985 Size: 6181 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6181 BstYI r'GATC_y Cuts at: 1972 2231 3395 3418 3511 3878 4263 4280 5732 Size: 259 1164 23 93 367 385 17 1452 Cuts at: 5732 5818 5830 6176 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1164 385 367 346 259 93 86 23 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5629 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5442 Fragments arranged by size: 5442 683 56 BtsI GCAGTG_nn' Cuts at: 3697 4491 4511 3697 Size: 794 20 5367 Fragments arranged by size: 5367 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6181 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6146 DraI TTT'AAA Cuts at: 3485 3485 Size: 6181 DraIII CAC_nnn'GTG Cuts at: 2963 2963 Size: 6181 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2086 5188 809 Size: 507 770 3102 1802 Fragments arranged by size: 3102 1802 770 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2888 3029 3067 Size: 54 1425 50 275 18 1058 141 38 Cuts at: 3067 3628 4517 6146 8 Size: 561 889 1629 43 Fragments arranged by size: 1629 1425 1058 889 561 275 141 54 50 43 38 18 EagI C'GGCC_G Cuts at: 1537 1812 3628 1537 Size: 275 1816 4090 Fragments arranged by size: 4090 1816 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2041 2571 3309 4111 1353 Size: 688 530 738 802 3423 Fragments arranged by size: 3423 802 738 688 530 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2644 4768 5142 5288 387 Size: 1064 138 1055 2124 374 146 1280 Fragments arranged by size: 2124 1280 1064 1055 374 146 138 EcoICRI GAG'CTC Cuts at: 3016 3016 Size: 6181 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 3351 3420 3450 4244 4841 5628 Size: 393 440 1279 69 30 794 597 787 Cuts at: 5628 5896 1239 Size: 268 1524 Fragments arranged by size: 1524 1279 794 787 597 440 393 268 69 30 EcoO109I rG'GnC_Cy Cuts at: 2072 2072 Size: 6181 EcoRI G'AATT_C Cuts at: 3490 3868 3490 Size: 378 5803 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2077 2077 Size: 6181 FspI TGC'GCA Cuts at: 4687 4687 Size: 6181 HaeII r_GCGC'y Cuts at: 1678 5328 1678 Size: 3650 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2483 2515 2768 2800 2801 Size: 32 135 32 911 32 253 32 1 Cuts at: 2801 2833 3451 3483 4822 4854 4896 4928 1373 Size: 32 618 32 1339 32 42 32 2626 Fragments arranged by size: 2626 1339 911 618 253 135 42 32 32 32 32 32 32 32 32 1 HincII GTy'rAC Cuts at: 669 1657 1938 3525 3771 669 Size: 988 281 1587 246 3079 Fragments arranged by size: 3079 1587 988 281 246 HindIII A'AGCT_T Cuts at: 748 3432 748 Size: 2684 3497 HpaI GTT'AAC Cuts at: 3771 3771 Size: 6181 KpnI G_GTAC'C Cuts at: 3506 3506 Size: 6181 MfeI C'AATT_G Cuts at: 3780 3780 Size: 6181 MluI A'CGCG_T Cuts at: 6057 6057 Size: 6181 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5295 5479 1305 Size: 3990 184 2007 Fragments arranged by size: 3990 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3031 3069 10 Size: 54 1425 343 1199 38 3122 Fragments arranged by size: 3122 1425 1199 343 54 38 MslI CAynn'nnrTG Cuts at: 518 1166 3179 4139 4498 4657 518 Size: 648 2013 960 359 159 2042 Fragments arranged by size: 2042 2013 960 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3597 4274 5422 5667 1606 Size: 1991 677 1148 245 2120 Fragments arranged by size: 2120 1991 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5629 NdeI CA'TA_TG Cuts at: 387 387 Size: 6181 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6181 NotI GC'GGCC_GC Cuts at: 3628 3628 Size: 6181 NspI r_CATG'y Cuts at: 1132 1229 2641 2793 2815 3539 5084 1132 Size: 97 1412 152 22 724 1545 2229 Fragments arranged by size: 2229 1545 1412 724 152 97 22 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6181 PciI A'CATG_T Cuts at: 5080 5080 Size: 6181 PflMI CCAn_nnn'nTGG Cuts at: 1108 2151 2917 1108 Size: 1043 766 4372 Fragments arranged by size: 4372 1043 766 PmeI GTTT'AAAC Cuts at: 3485 3485 Size: 6181 PmlI CAC'GTG Cuts at: 2465 2465 Size: 6181 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2825 2857 4250 4282 5763 5795 2825 Size: 32 1393 32 1481 32 3211 Fragments arranged by size: 3211 1481 1393 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6181 PsiI TTA'TAA Cuts at: 3751 3751 Size: 6181 PstI C_TGCA'G Cuts at: 830 3533 830 Size: 2703 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6181 SacI G_AGCT'C Cuts at: 3018 3018 Size: 6181 SalI G'TCGA_C Cuts at: 1936 3523 1936 Size: 1587 4594 SapI GCTCTTCn'nnn_ Cuts at: 3309 3309 Size: 6181 SbfI CC_TGCA'GG Cuts at: 3533 3533 Size: 6181 ScaI AGT'ACT Cuts at: 2408 4429 6141 2408 Size: 2021 1712 2448 Fragments arranged by size: 2448 2021 1712 SexAI A'CCwGG_T Cuts at: 3000 3000 Size: 6181 SfcI C'TryA_G Cuts at: 826 1045 2185 3529 4664 5345 5536 826 Size: 219 1140 1344 1135 681 191 1471 Fragments arranged by size: 1471 1344 1140 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6181 SmaI CCC'GGG Cuts at: 1872 3284 3508 1872 Size: 1412 224 4545 Fragments arranged by size: 4545 1412 224 SmlI C'TyrA_G Cuts at: 820 1017 1943 2642 4285 5186 5448 820 Size: 197 926 699 1643 901 262 1553 Fragments arranged by size: 1643 1553 926 901 699 262 197 SnaBI TAC'GTA Cuts at: 493 3972 493 Size: 3479 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6181 SphI G_CATG'C Cuts at: 1132 2641 3539 1132 Size: 1509 898 3774 Fragments arranged by size: 3774 1509 898 SspI AAT'ATT Cuts at: 5 52 4105 5 Size: 47 4053 2081 Fragments arranged by size: 4053 2081 47 StuI AGG'CCT Cuts at: 1517 2748 3165 1517 Size: 1231 417 4533 Fragments arranged by size: 4533 1231 417 StyI C'CwwG_G Cuts at: 513 1065 3620 513 Size: 552 2555 3074 Fragments arranged by size: 3074 2555 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2974 4208 4225 4378 4563 5008 325 Size: 1255 1394 1234 17 153 185 445 1498 Fragments arranged by size: 1498 1394 1255 1234 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2406 2503 2566 Size: 274 80 33 51 1443 429 97 63 Cuts at: 2566 4427 6139 96 Size: 1861 1712 138 Fragments arranged by size: 1861 1712 1443 429 274 138 97 80 63 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6141 6173 6141 Size: 32 6149 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6181 XbaI T'CTAG_A Cuts at: 3517 3517 Size: 6181 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6181 XmaI C'CCGG_G Cuts at: 1870 3282 3506 1870 Size: 1412 224 4545 Fragments arranged by size: 4545 1412 224 XmnI GAAnn'nnTTC Cuts at: 2337 4310 2337 Size: 1973 4208 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3989 276 Size: 53 83 186 936 2455 2468 Fragments arranged by size: 2468 2455 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI