(Circular) (Six-base) MAPSORT of: pFN21AB7047.seq Check: 5197 from: 1 to: 6175 pFN21AB7047 6175 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3985 278 Size: 53 83 186 936 2449 2468 Fragments arranged by size: 2468 2449 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2313 3518 1937 Size: 376 1205 4594 Fragments arranged by size: 4594 1205 376 Acc65I G'GTAC_C Cuts at: 3496 3496 Size: 6175 AclI AA'CG_TT Cuts at: 1563 3474 4302 4675 1563 Size: 1911 828 373 3063 Fragments arranged by size: 3063 1911 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2256 2465 2697 2919 3089 3147 3339 4238 Size: 624 209 232 222 170 58 192 899 Cuts at: 4238 5622 5890 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 899 624 268 232 222 209 192 170 58 AfeI AGC'GCT Cuts at: 3092 3092 Size: 6175 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5978 AflIII A'CryG_T Cuts at: 1203 5074 6051 1203 Size: 3871 977 1327 Fragments arranged by size: 3871 1327 977 AgeI A'CCGG_T Cuts at: 5006 5006 Size: 6175 AhdI GACnn_n'nnGTC Cuts at: 4904 4904 Size: 6175 AleI CACnn'nnGTG Cuts at: 1166 3137 1166 Size: 1971 4204 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2274 1184 Size: 1090 5085 AlwNI CAG_nnn'CTG Cuts at: 3386 3918 5490 3386 Size: 532 1572 4071 Fragments arranged by size: 4071 1572 532 ApaLI G'TGCA_C Cuts at: 4232 5388 4232 Size: 1156 5019 ApoI r'AATT_y Cuts at: 1493 2023 2132 2381 2516 2750 3484 3710 3862 Size: 530 109 249 135 234 734 226 152 Cuts at: 3862 5971 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 734 530 249 234 226 152 135 109 AseI AT'TA_AT Cuts at: 160 1032 3272 4729 160 Size: 872 2240 1457 1606 Fragments arranged by size: 2240 1606 1457 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 AvaI C'yCGr_G Cuts at: 1870 1943 3500 1870 Size: 73 1557 4545 Fragments arranged by size: 4545 1557 73 BamHI G'GATC_C Cuts at: 3505 3872 3505 Size: 367 5808 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3496 4951 618 Size: 325 217 627 1709 1455 1842 Fragments arranged by size: 1842 1709 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3450 3494 1391 Size: 2059 44 4072 Fragments arranged by size: 4072 2059 44 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2091 3135 928 Size: 971 192 1044 3968 Fragments arranged by size: 3968 1044 971 192 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4366 4400 1903 Size: 34 2429 34 3678 Fragments arranged by size: 3678 2429 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4067 5277 4067 Size: 1210 4965 BclI T'GATC_A Cuts at: 1548 1548 Size: 6175 BfrBI ATG'CAT Cuts at: 2996 2996 Size: 6175 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4786 136 Size: 107 122 71 631 605 3114 1525 Fragments arranged by size: 3114 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3292 6170 3292 Size: 2878 3297 BlpI GC'TnA_GC Cuts at: 3592 3592 Size: 6175 Bme1580I G_kGCm'C Cuts at: 1790 4236 5392 1790 Size: 2446 1156 2573 Fragments arranged by size: 2573 2446 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6175 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3338 3906 4864 Size: 416 53 83 399 24 1921 568 958 Cuts at: 4864 442 Size: 1753 Fragments arranged by size: 1921 1753 958 568 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6175 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3467 3469 3467 Size: 2 6173 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2051 3041 3418 4835 1239 Size: 812 990 377 1417 2579 Fragments arranged by size: 2579 1417 990 812 377 Bpu10I CC'TnA_GC Cuts at: 3341 3341 Size: 6175 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2080 2990 3413 4300 5165 5463 2080 Size: 910 423 887 865 298 2792 Fragments arranged by size: 2792 910 887 865 423 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4838 882 Size: 617 3339 2219 Fragments arranged by size: 3339 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3966 493 Size: 711 2762 2702 Fragments arranged by size: 2762 2702 711 BsaBI GATnn'nnATC Cuts at: 6169 6169 Size: 6175 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3982 4364 4999 275 Size: 53 83 186 936 2449 382 635 1451 Fragments arranged by size: 2449 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4608 5006 5280 5427 5838 4608 Size: 398 274 147 411 4945 Fragments arranged by size: 4945 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5950 Fragments arranged by size: 5950 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2512 3440 1186 Size: 1326 928 3921 Fragments arranged by size: 3921 1326 928 BseYI C'CCAG_C Cuts at: 5378 5874 5378 Size: 496 5679 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2763 1163 Size: 573 1027 4575 Fragments arranged by size: 4575 1027 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3625 4386 5414 1540 Size: 275 186 1624 761 1028 2301 Fragments arranged by size: 2301 1624 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3450 4236 4321 5392 3450 Size: 786 85 1071 4233 Fragments arranged by size: 4233 1071 786 85 BsmI GAATG_Cn' Cuts at: 2225 2998 3686 3779 2225 Size: 773 688 93 4621 Fragments arranged by size: 4621 773 688 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3017 3017 Size: 6175 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3450 3494 4236 4321 5392 1391 Size: 399 1660 44 742 85 1071 2174 Fragments arranged by size: 2174 1660 1071 742 399 85 44 BspEI T'CCGG_A Cuts at: 5838 5838 Size: 6175 BspHI T'CATG_A Cuts at: 4064 5794 4064 Size: 1730 4445 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2381 3530 844 Size: 1537 1149 3489 Fragments arranged by size: 3489 1537 1149 BsrBI CCG'CTC Cuts at: 1394 1823 4062 1394 Size: 429 2239 3507 Fragments arranged by size: 3507 2239 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4670 4844 65 Size: 1183 3422 174 1396 Fragments arranged by size: 3422 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4819 5006 1700 Size: 110 3009 187 2869 Fragments arranged by size: 3009 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2163 96 Size: 2067 4108 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6175 BssSI C'ACGA_G Cuts at: 4235 5247 4235 Size: 1012 5163 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6175 BstBI TT'CG_AA Cuts at: 2957 4979 2957 Size: 2022 4153 BstEII G'GTnAC_C Cuts at: 1184 2014 2768 1184 Size: 830 754 4591 Fragments arranged by size: 4591 830 754 BstXI CCAn_nnnn'nTGG Cuts at: 3255 3255 Size: 6175 BstYI r'GATC_y Cuts at: 1972 2101 3292 3505 3872 4257 4274 5726 5812 Size: 129 1191 213 367 385 17 1452 86 Cuts at: 5812 5824 6170 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1191 385 367 346 213 129 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5623 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2555 487 Size: 683 56 1329 4107 Fragments arranged by size: 4107 1329 683 56 BtsI GCAGTG_nn' Cuts at: 2746 3132 3691 4485 4505 2746 Size: 386 559 794 20 4416 Fragments arranged by size: 4416 794 559 386 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6175 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6140 DraI TTT'AAA Cuts at: 2139 3479 2139 Size: 1340 4835 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5182 809 Size: 507 3866 1802 Fragments arranged by size: 3866 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3622 4511 6140 Size: 54 1425 50 275 18 1792 889 1629 Cuts at: 6140 8 Size: 43 Fragments arranged by size: 1792 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3622 1537 Size: 275 1810 4090 Fragments arranged by size: 4090 1810 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2232 3186 4105 1353 Size: 879 954 919 3423 Fragments arranged by size: 3423 954 919 879 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4762 5136 5282 387 Size: 1064 138 3173 374 146 1280 Fragments arranged by size: 3173 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3448 3448 Size: 6175 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2256 2465 2697 2919 3041 3089 Size: 393 419 205 209 232 222 122 48 Cuts at: 3089 3147 3339 3418 4238 4835 5622 5890 1239 Size: 58 192 79 820 597 787 268 1524 Fragments arranged by size: 1524 820 787 597 419 393 268 232 222 209 205 192 122 79 58 48 EcoRI G'AATT_C Cuts at: 2023 2516 3484 3862 2023 Size: 493 968 378 4336 Fragments arranged by size: 4336 968 493 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2656 2656 Size: 6175 FspI TGC'GCA Cuts at: 4681 4681 Size: 6175 HaeII r_GCGC'y Cuts at: 1678 3094 5322 1678 Size: 1416 2228 2531 Fragments arranged by size: 2531 2228 1416 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2078 2110 2533 2565 3146 Size: 32 135 32 506 32 423 32 581 Cuts at: 3146 3178 4816 4848 4890 4922 1373 Size: 32 1638 32 42 32 2626 Fragments arranged by size: 2626 1638 581 506 423 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3519 3765 669 Size: 988 281 1581 246 3079 Fragments arranged by size: 3079 1581 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6175 HpaI GTT'AAC Cuts at: 3765 3765 Size: 6175 KpnI G_GTAC'C Cuts at: 3500 3500 Size: 6175 MfeI C'AATT_G Cuts at: 2535 3774 2535 Size: 1239 4936 MluI A'CGCG_T Cuts at: 6051 6051 Size: 6175 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2787 5289 5473 1305 Size: 1482 2502 184 2007 Fragments arranged by size: 2502 2007 1482 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4353 Fragments arranged by size: 4353 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2193 3137 4133 4492 4651 518 Size: 648 1027 944 996 359 159 2042 Fragments arranged by size: 2042 1027 996 944 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2050 3591 4268 5416 5661 1606 Size: 444 1541 677 1148 245 2120 Fragments arranged by size: 2120 1541 1148 677 444 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5623 NdeI CA'TA_TG Cuts at: 387 387 Size: 6175 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6175 NotI GC'GGCC_GC Cuts at: 3622 3622 Size: 6175 NsiI A_TGCA'T Cuts at: 2998 2998 Size: 6175 NspI r_CATG'y Cuts at: 1132 1229 2261 3533 5078 1132 Size: 97 1032 1272 1545 2229 Fragments arranged by size: 2229 1545 1272 1032 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6175 PciI A'CATG_T Cuts at: 5074 5074 Size: 6175 PflMI CCAn_nnn'nTGG Cuts at: 1108 2825 1108 Size: 1717 4458 PfoI T'CCnGG_A Cuts at: 3018 3018 Size: 6175 PmeI GTTT'AAAC Cuts at: 3479 3479 Size: 6175 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2558 2590 4244 4276 5757 5789 2558 Size: 32 1654 32 1481 32 2944 Fragments arranged by size: 2944 1654 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6175 PsiI TTA'TAA Cuts at: 3745 3745 Size: 6175 PstI C_TGCA'G Cuts at: 830 2658 3527 830 Size: 1828 869 3478 Fragments arranged by size: 3478 1828 869 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6175 PvuII CAG'CTG Cuts at: 2050 2050 Size: 6175 SacI G_AGCT'C Cuts at: 3450 3450 Size: 6175 SalI G'TCGA_C Cuts at: 1936 3517 1936 Size: 1581 4594 SbfI CC_TGCA'GG Cuts at: 3527 3527 Size: 6175 ScaI AGT'ACT Cuts at: 4423 6135 4423 Size: 1712 4463 SfcI C'TryA_G Cuts at: 826 1045 2654 3523 4658 5339 5530 826 Size: 219 1609 869 1135 681 191 1471 Fragments arranged by size: 1609 1471 1135 869 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6175 SmaI CCC'GGG Cuts at: 1872 3502 1872 Size: 1630 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2095 2969 3392 4279 5180 5442 Size: 197 926 152 874 423 887 901 262 Cuts at: 5442 820 Size: 1553 Fragments arranged by size: 1553 926 901 887 874 423 262 197 152 SnaBI TAC'GTA Cuts at: 493 3966 493 Size: 3473 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6175 SphI G_CATG'C Cuts at: 1132 3533 1132 Size: 2401 3774 SspI AAT'ATT Cuts at: 5 52 4099 5 Size: 47 4047 2081 Fragments arranged by size: 4047 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6175 StyI C'CwwG_G Cuts at: 513 1065 2703 3614 513 Size: 552 1638 911 3074 Fragments arranged by size: 3074 1638 911 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2577 2838 4202 4219 4372 4557 5002 Size: 1255 997 261 1364 17 153 185 445 Cuts at: 5002 325 Size: 1498 Fragments arranged by size: 1498 1364 1255 997 445 261 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2163 4421 6133 Size: 274 80 33 51 1443 186 2258 1712 Cuts at: 6133 96 Size: 138 Fragments arranged by size: 2258 1712 1443 274 186 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6135 6167 6135 Size: 32 6143 Tth111I GACn'n_nGTC Cuts at: 1316 2090 1316 Size: 774 5401 XbaI T'CTAG_A Cuts at: 2104 3511 2104 Size: 1407 4768 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2026 2026 Size: 6175 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6175 XmaI C'CCGG_G Cuts at: 1870 3500 1870 Size: 1630 4545 XmnI GAAnn'nnTTC Cuts at: 2075 2573 2793 4304 2075 Size: 498 220 1511 3946 Fragments arranged by size: 3946 1511 498 220 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3983 276 Size: 53 83 186 936 2449 2468 Fragments arranged by size: 2468 2449 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI