Restriction map for
pFN21AB7045
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII AccII AccIII AclI AcsI
AflII AflIII AhdI AlwNI AocI
Aor51HI ApoI AscI AseI AsnI
AspEI AspI AvaI AvrII BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BfrI BglII BlnI BpuAI
BsaAI BsaBI BseAI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstUI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FseI
HincII HindII HindIII HpaI KasI
KspI MamI MfeI MluI MroI
MseI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PleI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SwaI TaqI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 1351 GGTACC G^GTACC
AccI 19 GTAGAC GT^[AC][GT]AC
AcyI 996 GACGCC G[GA]^CG[CT]C
AgeI 1177 ACCGGT A^CCGGT
ApaI 822 GGGCCC GGGCC^C
Asp700I 1185 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1351 GGTACC G^GTACC
AviII 1127 TGCGCA TGC^GCA
BalI 431 TGGCCA TGG^CCA
BanII 822 GGGCCC G[GA]GC[CT]^C
BclI 176 TGATCA T^GATCA
BfaI 254 CTAG C^TAG
BglI 945 GCCACTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 893 GCTCAGC GC^T[ACGT]AGC
BpmI 194 CTGGAG CTGGAG(16/14)
Bpu1102I 893 GCTCAGC GC^T[ACGT]AGC
BsaHI 996 GACGCC G[GA]^CG[CT]C
BsiEI 1041 CGACCG CG[GA][CT]^CG
BsmBI 243 CGTCTC CGTCTC(1/5)
BspMI 941 ACCTGC ACCTGC(4/8)
BssSI 1006 CACGAG CACGAG(-5/-1)
BstXI 422 CCAACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 893 GCTCAGC GC^T[ACGT]AGC
DsaI 641 CCACGG C^C[GA][CT]GG
EcoNI 540 CCTCTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 11 CCAAGG C^C[AT][AT]GG
FbaI 176 TGATCA T^GATCA
FspI 1127 TGCGCA TGC^GCA
HaeII 1241 AGCGCC [GA]GCGC^[CT]
Hin1I 996 GACGCC G[GA]^CG[CT]C
KpnI 1351 GGTACC GGTAC^C
MaeI 254 CTAG C^TAG
MaeII 100 ACGT A^CGT
MluNI 431 TGGCCA TGG^CCA
MscI 431 TGGCCA TGG^CCA
NspI 1124 GCATGC [GA]CATG^[CT]
PinAI 1177 ACCGGT A^CCGGT
PshAI 646 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfcI 1316 CTACAG C^T[GA][CT]AG
SphI 1124 GCATGC GCATG^C
SspI 340 AATATT AAT^ATT
StuI 964 AGGCCT AGG^CCT
StyI 11 CCAAGG C^C[AT][AT]GG
XmnI 1185 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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BsaWI 841 ACCGGA [AT]^CCGG[AT]
1177 ACCGGT
BsmFI 870 GGGAC GGGAC(10/14)
462 GTCCC
BstEII 105 GGTGACC G^GT[ACGT]ACC
837 GGTGACC
BstPI 105 GGTGACC G^GT[ACGT]ACC
837 GGTGACC
CfoI 1128 GCGC GCG^C
1242 GCGC
EcoO65I 105 GGTGACC G^GT[ACGT]ACC
837 GGTGACC
FauI 1055 CCCGC CCCGC(4/6)
768 GCGGG
HgaI 996 GACGC GACGC(5/10)
404 GCGTC
HhaI 1128 GCGC GCG^C
1242 GCGC
HinP1I 1128 GCGC G^CGC
1242 GCGC
HinfI 683 GATTC G^A[ACGT]TC
1085 GAATC
SfaNI 356 GCATC GCATC(5/9)
1297 GCATC
SgrAI 998 CGCCGGTG C[GA]^CCGG[CT]G
1176 CACCGGTG
TfiI 683 GATTC G^A[AT]TC
1085 GAATC
XcmI 119 CCAATGCCGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
434 CCACAGGACCCCTGG
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI Alw44I AlwI
ApaLI AspHI AvaII BanI BbvI
BcnI BmyI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI TseI Tsp45I TspRI XhoII