(Circular) (Six-base) MAPSORT of: pFN21AB7045.seq Check: 2190 from: 1 to: 6133 pFN21AB7045 6133 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2955 2955 Size: 6133 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3943 278 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2025 3476 1937 Size: 88 1451 4594 Fragments arranged by size: 4594 1451 88 Acc65I G'GTAC_C Cuts at: 3356 3454 3356 Size: 98 6035 AclI AA'CG_TT Cuts at: 1563 4260 4633 1563 Size: 2697 373 3063 Fragments arranged by size: 3063 2697 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3124 4196 5580 5848 1632 Size: 1492 1072 1384 268 1917 Fragments arranged by size: 1917 1492 1384 1072 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5936 AflIII A'CryG_T Cuts at: 1203 5032 6009 1203 Size: 3829 977 1327 Fragments arranged by size: 3829 1327 977 AgeI A'CCGG_T Cuts at: 3182 4964 3182 Size: 1782 4351 AhdI GACnn_n'nnGTC Cuts at: 4862 4862 Size: 6133 AleI CACnn'nnGTG Cuts at: 1166 2108 3183 1166 Size: 942 1075 4116 Fragments arranged by size: 4116 1075 942 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3058 1184 Size: 1874 4259 AlwNI CAG_nnn'CTG Cuts at: 3876 5448 3876 Size: 1572 4561 ApaI G_GGCC'C Cuts at: 2831 2831 Size: 6133 ApaLI G'TGCA_C Cuts at: 2057 2458 3008 4190 5346 2057 Size: 401 550 1182 1156 2844 Fragments arranged by size: 2844 1182 1156 550 401 ApoI r'AATT_y Cuts at: 1493 3442 3668 3820 5929 1493 Size: 1949 226 152 2109 1697 Fragments arranged by size: 2109 1949 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4687 160 Size: 872 3655 1606 Fragments arranged by size: 3655 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 AvaI C'yCGr_G Cuts at: 1870 1943 3458 1870 Size: 73 1515 4545 Fragments arranged by size: 4545 1515 73 BamHI G'GATC_C Cuts at: 3463 3830 3463 Size: 367 5766 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2315 2776 3307 3356 3454 Size: 325 217 627 528 461 531 49 98 Cuts at: 3454 4909 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 531 528 461 325 217 98 49 BanII G_rGCy'C Cuts at: 1391 2831 3452 1391 Size: 1440 621 4072 Fragments arranged by size: 4072 1440 621 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5162 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4324 4358 1903 Size: 34 2387 34 3678 Fragments arranged by size: 3678 2387 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4025 5235 4025 Size: 1210 4923 BclI T'GATC_A Cuts at: 1548 2181 1548 Size: 633 5500 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2956 4744 136 Size: 107 122 71 631 605 1284 1788 1525 Fragments arranged by size: 1788 1525 1284 631 605 122 107 71 BglII A'GATC_T Cuts at: 6128 6128 Size: 6133 BlpI GC'TnA_GC Cuts at: 2899 3550 2899 Size: 651 5482 Bme1580I G_kGCm'C Cuts at: 1790 2061 2462 2781 2831 3012 3032 3295 4194 Size: 271 401 319 50 181 20 263 899 Cuts at: 4194 5350 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 899 401 319 271 263 181 50 20 BmgBI CAC'GTC Cuts at: 1318 2106 1318 Size: 788 5345 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2030 2144 2826 Size: 416 53 83 399 24 613 114 682 Cuts at: 2826 3108 3864 4822 442 Size: 282 756 958 1753 Fragments arranged by size: 1753 958 756 682 613 416 399 282 114 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6133 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3416 3416 Size: 6133 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2220 4793 1239 Size: 981 2573 2579 Fragments arranged by size: 2579 2573 981 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4258 5123 5421 4258 Size: 865 298 4970 Fragments arranged by size: 4970 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2535 2663 2706 4796 882 Size: 617 1036 128 43 2090 2219 Fragments arranged by size: 2219 2090 1036 617 128 43 BsaAI yAC'GTr Cuts at: 493 1204 3924 493 Size: 711 2720 2702 Fragments arranged by size: 2720 2702 711 BsaBI GATnn'nnATC Cuts at: 6127 6127 Size: 6133 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3002 3940 4322 4957 Size: 53 83 186 936 1469 938 382 635 Cuts at: 4957 275 Size: 1451 Fragments arranged by size: 1469 1451 938 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2846 3182 4566 4964 5238 5385 5796 2846 Size: 336 1384 398 274 147 411 3183 Fragments arranged by size: 3183 1384 411 398 336 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5908 Fragments arranged by size: 5908 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2505 2508 3403 1186 Size: 1319 3 895 3916 Fragments arranged by size: 3916 1319 895 3 BseYI C'CCAG_C Cuts at: 2231 2383 2475 2837 2993 3031 5336 5832 2231 Size: 152 92 362 156 38 2305 496 2532 Fragments arranged by size: 2532 2305 496 362 156 152 92 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2238 2376 2556 3365 3385 1163 Size: 573 502 138 180 809 20 3911 Fragments arranged by size: 3911 809 573 502 180 138 20 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3049 3583 4344 5372 1540 Size: 275 186 1048 534 761 1028 2301 Fragments arranged by size: 2301 1048 1028 761 534 275 186 BsiHKAI G_wGCw'C Cuts at: 2061 2462 3012 4194 4279 5350 2061 Size: 401 550 1182 85 1071 2844 Fragments arranged by size: 2844 1182 1071 550 401 85 BsmI GAATG_Cn' Cuts at: 3644 3737 3644 Size: 93 6040 BsmBI CGTCTCn'nnnn_ Cuts at: 2254 2254 Size: 6133 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2462 2781 2831 3012 3032 3295 Size: 399 271 401 319 50 181 20 263 Cuts at: 3295 3452 4194 4279 5350 1391 Size: 157 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 401 399 319 271 263 181 157 85 50 20 BspEI T'CCGG_A Cuts at: 5796 5796 Size: 6133 BspHI T'CATG_A Cuts at: 4022 5752 4022 Size: 1730 4403 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2955 3488 844 Size: 2111 533 3489 Fragments arranged by size: 3489 2111 533 BsrBI CCG'CTC Cuts at: 1394 1823 3063 3280 3400 4020 1394 Size: 429 1240 217 120 620 3507 Fragments arranged by size: 3507 1240 620 429 217 120 BsrDI GCAATG_nn' Cuts at: 65 1248 4628 4802 65 Size: 1183 3380 174 1396 Fragments arranged by size: 3380 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3004 3048 3182 4777 4964 1700 Size: 110 1194 44 134 1595 187 2869 Fragments arranged by size: 2869 1595 1194 187 134 110 44 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6133 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6133 BssSI C'ACGA_G Cuts at: 3011 4193 5205 3011 Size: 1182 1012 3939 Fragments arranged by size: 3939 1182 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6133 BstBI TT'CG_AA Cuts at: 4937 4937 Size: 6133 BstEII G'GTnAC_C Cuts at: 1184 2110 2842 1184 Size: 926 732 4475 Fragments arranged by size: 4475 926 732 BstXI CCAn_nnnn'nTGG Cuts at: 2434 2434 Size: 6133 BstYI r'GATC_y Cuts at: 1972 2218 3123 3258 3463 3830 4215 4232 5684 Size: 246 905 135 205 367 385 17 1452 Cuts at: 5684 5770 5782 6128 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 905 385 367 346 246 205 135 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2646 513 Size: 552 1581 4000 Fragments arranged by size: 4000 1581 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5394 Fragments arranged by size: 5394 683 56 BtsI GCAGTG_nn' Cuts at: 2951 3154 3295 3649 4443 4463 2951 Size: 203 141 354 794 20 4621 Fragments arranged by size: 4621 794 354 203 141 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6133 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6098 DraI TTT'AAA Cuts at: 3437 3437 Size: 6133 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5140 809 Size: 507 3824 1802 Fragments arranged by size: 3824 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2166 2436 3214 Size: 54 1425 50 275 18 336 270 778 Cuts at: 3214 3580 4469 6098 8 Size: 366 889 1629 43 Fragments arranged by size: 1629 1425 889 778 366 336 275 270 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3580 1537 Size: 275 1768 4090 Fragments arranged by size: 4090 1768 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2201 2599 2666 4063 1353 Size: 848 398 67 1397 3423 Fragments arranged by size: 3423 1397 848 398 67 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2493 3405 4720 5094 5240 387 Size: 1064 138 904 912 1315 374 146 1280 Fragments arranged by size: 1315 1280 1064 912 904 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2220 3124 4196 4793 5580 5848 1239 Size: 393 588 904 1072 597 787 268 1524 Fragments arranged by size: 1524 1072 904 787 597 588 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2549 2549 Size: 6133 EcoO109I rG'GnC_Cy Cuts at: 2092 2141 2444 2554 2827 2828 2876 3018 2092 Size: 49 303 110 273 1 48 142 5207 Fragments arranged by size: 5207 303 273 142 110 49 48 1 EcoRI G'AATT_C Cuts at: 3442 3820 3442 Size: 378 5755 FspI TGC'GCA Cuts at: 3134 4639 3134 Size: 1505 4628 HaeII r_GCGC'y Cuts at: 1678 3250 5280 1678 Size: 1572 2030 2531 Fragments arranged by size: 2531 2030 1572 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2195 2227 4774 4806 4848 Size: 32 135 32 623 32 2547 32 42 Cuts at: 4848 4880 1373 Size: 32 2626 Fragments arranged by size: 2626 2547 623 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3477 3723 669 Size: 988 281 1539 246 3079 Fragments arranged by size: 3079 1539 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6133 HpaI GTT'AAC Cuts at: 3723 3723 Size: 6133 KpnI G_GTAC'C Cuts at: 3360 3458 3360 Size: 98 6035 MfeI C'AATT_G Cuts at: 3732 3732 Size: 6133 MluI A'CGCG_T Cuts at: 6009 6009 Size: 6133 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2196 3101 5247 5431 1305 Size: 891 905 2146 184 2007 Fragments arranged by size: 2146 2007 905 891 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2438 10 Size: 54 1425 343 606 3705 Fragments arranged by size: 3705 1425 606 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2108 2192 2279 2701 3183 4091 4450 Size: 648 942 84 87 422 482 908 359 Cuts at: 4450 4609 518 Size: 159 2042 Fragments arranged by size: 2042 942 908 648 482 422 359 159 87 84 MspA1I CmG'CkG Cuts at: 1606 2065 2322 2729 3549 4226 5374 5619 1606 Size: 459 257 407 820 677 1148 245 2120 Fragments arranged by size: 2120 1148 820 677 459 407 257 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5581 NdeI CA'TA_TG Cuts at: 387 387 Size: 6133 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6133 NotI GC'GGCC_GC Cuts at: 3580 3580 Size: 6133 NspI r_CATG'y Cuts at: 1132 1229 3133 3491 5036 1132 Size: 97 1904 358 1545 2229 Fragments arranged by size: 2229 1904 1545 358 97 PasI CC'CwG_GG Cuts at: 1348 2449 2821 1348 Size: 1101 372 4660 Fragments arranged by size: 4660 1101 372 PciI A'CATG_T Cuts at: 5032 5032 Size: 6133 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6133 PfoI T'CCnGG_A Cuts at: 2683 2683 Size: 6133 PmeI GTTT'AAAC Cuts at: 3437 3437 Size: 6133 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4202 4234 5715 5747 4202 Size: 32 1481 32 4588 Fragments arranged by size: 4588 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2092 2444 2554 2876 2092 Size: 352 110 322 5349 Fragments arranged by size: 5349 352 322 110 PshAI GACnn'nnGTC Cuts at: 1613 2655 1613 Size: 1042 5091 PsiI TTA'TAA Cuts at: 3703 3703 Size: 6133 PspOMI G'GGCC_C Cuts at: 2827 2827 Size: 6133 PstI C_TGCA'G Cuts at: 830 3485 830 Size: 2655 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6133 PvuII CAG'CTG Cuts at: 2065 2322 2729 2065 Size: 257 407 5469 Fragments arranged by size: 5469 407 257 SalI G'TCGA_C Cuts at: 1936 3475 1936 Size: 1539 4594 SanDI GG'GwC_CC Cuts at: 2876 2876 Size: 6133 SbfI CC_TGCA'GG Cuts at: 3485 3485 Size: 6133 ScaI AGT'ACT Cuts at: 4381 6093 4381 Size: 1712 4421 SfcI C'TryA_G Cuts at: 826 1045 3321 3481 4616 5297 5488 826 Size: 219 2276 160 1135 681 191 1471 Fragments arranged by size: 2276 1471 1135 681 219 191 160 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 SgrAI Cr'CCGG_yG Cuts at: 1700 3004 3182 1700 Size: 1304 178 4651 Fragments arranged by size: 4651 1304 178 SmaI CCC'GGG Cuts at: 1872 3460 1872 Size: 1588 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4237 5138 5400 820 Size: 197 926 2294 901 262 1553 Fragments arranged by size: 2294 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3924 493 Size: 3431 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6133 SphI G_CATG'C Cuts at: 1132 3133 3491 1132 Size: 2001 358 3774 Fragments arranged by size: 3774 2001 358 SspI AAT'ATT Cuts at: 5 52 2347 4057 5 Size: 47 2295 1710 2081 Fragments arranged by size: 2295 2081 1710 47 StuI AGG'CCT Cuts at: 1517 2971 1517 Size: 1454 4679 StyI C'CwwG_G Cuts at: 513 1065 2016 3572 513 Size: 552 951 1556 3074 Fragments arranged by size: 3074 1556 951 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2441 3179 3191 4160 4177 4330 4515 Size: 1255 861 738 12 969 17 153 185 Cuts at: 4515 4960 325 Size: 445 1498 Fragments arranged by size: 1498 1255 969 861 738 445 185 153 17 12 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2496 4379 6091 Size: 274 80 33 51 1443 519 1883 1712 Cuts at: 6091 96 Size: 138 Fragments arranged by size: 1883 1712 1443 519 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3069 3101 3274 3306 6093 6125 3069 Size: 32 173 32 2787 32 3077 Fragments arranged by size: 3077 2787 173 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6133 XbaI T'CTAG_A Cuts at: 3469 3469 Size: 6133 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2131 2446 2131 Size: 315 5818 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6133 XmaI C'CCGG_G Cuts at: 1870 3458 1870 Size: 1588 4545 XmnI GAAnn'nnTTC Cuts at: 3194 4262 3194 Size: 1068 5065 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3941 276 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI