Restriction map for
pFN21AB7045
(Linear) (Six-base) MAPSORT of: KIBB7045.seq Check: 1812 from: 1 to: 1428
KIBB7045.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 950 1428
Size: 950 478
AccI GT'mk_AC
Cuts at: 0 20 1428
Size: 20 1408
Acc65I G'GTAC_C
Cuts at: 0 1351 1428
Size: 1351 77
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1119 1428
Size: 1119 309
AgeI A'CCGG_T
Cuts at: 0 1177 1428
Size: 1177 251
AleI CACnn'nnGTG
Cuts at: 0 103 1178 1428
Size: 103 1075 250
Fragments arranged by size:
1075 250 103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1053 1428
Size: 1053 375
ApaI G_GGCC'C
Cuts at: 0 826 1428
Size: 826 602
ApaLI G'TGCA_C
Cuts at: 0 52 453 1003 1428
Size: 52 401 550 425
Fragments arranged by size:
550 425 401 52
BanI G'GyrC_C
Cuts at: 0 310 771 1302 1351 1428
Size: 310 461 531 49 77
Fragments arranged by size:
531 461 310 77 49
BanII G_rGCy'C
Cuts at: 0 826 1428
Size: 826 602
BclI T'GATC_A
Cuts at: 0 176 1428
Size: 176 1252
BglI GCCn_nnn'nGGC
Cuts at: 0 951 1428
Size: 951 477
BlpI GC'TnA_GC
Cuts at: 0 894 1428
Size: 894 534
Bme1580I G_kGCm'C
Cuts at: 0 56 457 776 826 1007 1027 1290 1428
Size: 56 401 319 50 181 20 263 138
Fragments arranged by size:
401 319 263 181 138 56 50 20
BmgBI CAC'GTC
Cuts at: 0 101 1428
Size: 101 1327
BmrI ACTGGGnnnn_n'
Cuts at: 0 25 139 821 1103 1428
Size: 25 114 682 282 325
Fragments arranged by size:
682 325 282 114 25
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1411 1428
Size: 1411 17
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 1428
Size: 215 1213
BsaI GGTCTCn'nnnn_
Cuts at: 0 530 658 701 1428
Size: 530 128 43 727
Fragments arranged by size:
727 530 128 43
BsaHI Gr'CG_yC
Cuts at: 0 997 1428
Size: 997 431
BsaWI w'CCGG_w
Cuts at: 0 841 1177 1428
Size: 841 336 251
Fragments arranged by size:
841 336 251
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 500 503 1398 1428
Size: 500 3 895 30
Fragments arranged by size:
895 500 30 3
BseYI C'CCAG_C
Cuts at: 0 226 378 470 832 988 1026 1428
Size: 226 152 92 362 156 38 402
Fragments arranged by size:
402 362 226 156 152 92 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 371 551 1360 1380 1428
Size: 233 138 180 809 20 48
Fragments arranged by size:
809 233 180 138 48 20
BsiEI CG_ry'CG
Cuts at: 0 1044 1428
Size: 1044 384
BsiHKAI G_wGCw'C
Cuts at: 0 56 457 1007 1428
Size: 56 401 550 421
Fragments arranged by size:
550 421 401 56
BsmBI CGTCTCn'nnnn_
Cuts at: 0 249 1428
Size: 249 1179
Bsp1286I G_dGCh'C
Cuts at: 0 56 457 776 826 1007 1027 1290 1428
Size: 56 401 319 50 181 20 263 138
Fragments arranged by size:
401 319 263 181 138 56 50 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 950 1428
Size: 950 478
BsrBI CCG'CTC
Cuts at: 0 1058 1275 1395 1428
Size: 1058 217 120 33
Fragments arranged by size:
1058 217 120 33
BsrFI r'CCGG_y
Cuts at: 0 999 1043 1177 1428
Size: 999 44 134 251
Fragments arranged by size:
999 251 134 44
BssSI C'ACGA_G
Cuts at: 0 1006 1428
Size: 1006 422
BstEII G'GTnAC_C
Cuts at: 0 105 837 1428
Size: 105 732 591
Fragments arranged by size:
732 591 105
BstXI CCAn_nnnn'nTGG
Cuts at: 0 429 1428
Size: 429 999
BstYI r'GATC_y
Cuts at: 0 213 1118 1253 1428
Size: 213 905 135 175
Fragments arranged by size:
905 213 175 135
BtgI C'CryG_G
Cuts at: 0 641 1428
Size: 641 787
BtsI GCAGTG_nn'
Cuts at: 0 946 1149 1290 1428
Size: 946 203 141 138
Fragments arranged by size:
946 203 141 138
EaeI y'GGCC_r
Cuts at: 0 161 431 1209 1428
Size: 161 270 778 219
Fragments arranged by size:
778 270 219 161
EarI CTCTTCn'nnn_
Cuts at: 0 196 594 661 1428
Size: 196 398 67 767
Fragments arranged by size:
767 398 196 67
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 488 1400 1428
Size: 488 912 28
Fragments arranged by size:
912 488 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 1119 1428
Size: 215 904 309
Fragments arranged by size:
904 309 215
EcoNI CCTnn'n_nnAGG
Cuts at: 0 544 1428
Size: 544 884
EcoO109I rG'GnC_Cy
Cuts at: 0 87 136 439 549 822 823 871 1013
Size: 87 49 303 110 273 1 48 142
Cuts at: 1013 1428
Size: 415
Fragments arranged by size:
415 303 273 142 110 87 49 48
1
FspI TGC'GCA
Cuts at: 0 1129 1428
Size: 1129 299
HaeII r_GCGC'y
Cuts at: 0 1245 1428
Size: 1245 183
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 190 222 1428
Size: 190 32 1206
Fragments arranged by size:
1206 190 32
KpnI G_GTAC'C
Cuts at: 0 1355 1428
Size: 1355 73
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 1096 1428
Size: 191 905 332
Fragments arranged by size:
905 332 191
MscI TGG'CCA
Cuts at: 0 433 1428
Size: 433 995
MslI CAynn'nnrTG
Cuts at: 0 103 187 274 696 1178 1428
Size: 103 84 87 422 482 250
Fragments arranged by size:
482 422 250 103 87 84
MspA1I CmG'CkG
Cuts at: 0 60 317 724 1428
Size: 60 257 407 704
Fragments arranged by size:
704 407 257 60
NspI r_CATG'y
Cuts at: 0 1128 1428
Size: 1128 300
PasI CC'CwG_GG
Cuts at: 0 444 816 1428
Size: 444 372 612
Fragments arranged by size:
612 444 372
PfoI T'CCnGG_A
Cuts at: 0 678 1428
Size: 678 750
PpuMI rG'GwC_Cy
Cuts at: 0 87 439 549 871 1428
Size: 87 352 110 322 557
Fragments arranged by size:
557 352 322 110 87
PshAI GACnn'nnGTC
Cuts at: 0 650 1428
Size: 650 778
PspOMI G'GGCC_C
Cuts at: 0 822 1428
Size: 822 606
PvuII CAG'CTG
Cuts at: 0 60 317 724 1428
Size: 60 257 407 704
Fragments arranged by size:
704 407 257 60
SanDI GG'GwC_CC
Cuts at: 0 871 1428
Size: 871 557
SfcI C'TryA_G
Cuts at: 0 1316 1428
Size: 1316 112
SgrAI Cr'CCGG_yG
Cuts at: 0 999 1177 1428
Size: 999 178 251
Fragments arranged by size:
999 251 178
SphI G_CATG'C
Cuts at: 0 1128 1428
Size: 1128 300
SspI AAT'ATT
Cuts at: 0 342 1428
Size: 342 1086
StuI AGG'CCT
Cuts at: 0 966 1428
Size: 966 462
StyI C'CwwG_G
Cuts at: 0 11 1428
Size: 11 1417
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 436 1174 1186 1428
Size: 436 738 12 242
Fragments arranged by size:
738 436 242 12
TatI w'GTAC_w
Cuts at: 0 491 1428
Size: 491 937
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1064 1096 1269 1301 1428
Size: 1064 32 173 32 127
Fragments arranged by size:
1064 173 127 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 126 441 1428
Size: 126 315 987
Fragments arranged by size:
987 315 126
XmnI GAAnn'nnTTC
Cuts at: 0 1189 1428
Size: 1189 239
Enzymes that do cut:
AarI AccI Acc65I AcuI AgeI AleI AlfI ApaI
ApaLI BanI BanII BclI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI BsaI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI
BssSI BstEII BstXI BstYI BtgI BtsI EaeI EarI
EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KpnI
MmeI MscI MslI MspA1I NspI PasI PfoI PpuMI
PshAI PspOMI PvuII SanDI SfcI SgrAI SphI SspI
StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AhdI AloI AlwNI
ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbeI BbsI BbvCI BcgI BciVI BfrBI BglII BmtI
Bpu10I BpuEI BsaAI BsaBI BsaXI BsiWI BsmI BspEI
BspHI BsrDI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI
KasI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PmeI
PmlI PpiI PsiI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SmaI SmlI SnaBI SpeI SrfI SwaI
Tth111I XbaI XhoI XmaI ZraI