(Circular) (Six-base) MAPSORT of: pFN21AB7039.seq Check: 6050 from: 1 to: 6079 pFN21AB7039 6079 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3889 278 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3422 1937 Size: 1485 4594 Acc65I G'GTAC_C Cuts at: 3400 3400 Size: 6079 AclI AA'CG_TT Cuts at: 1563 4206 4579 1563 Size: 2643 373 3063 Fragments arranged by size: 3063 2643 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2010 4142 5526 5794 1632 Size: 378 2132 1384 268 1917 Fragments arranged by size: 2132 1917 1384 378 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5882 AflIII A'CryG_T Cuts at: 1203 3207 4978 5955 1203 Size: 2004 1771 977 1327 Fragments arranged by size: 2004 1771 1327 977 AgeI A'CCGG_T Cuts at: 4910 4910 Size: 6079 AhdI GACnn_n'nnGTC Cuts at: 3123 4808 3123 Size: 1685 4394 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6079 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6079 AlwNI CAG_nnn'CTG Cuts at: 2344 2947 3822 5394 2344 Size: 603 875 1572 3029 Fragments arranged by size: 3029 1572 875 603 ApaLI G'TGCA_C Cuts at: 2227 4136 5292 2227 Size: 1909 1156 3014 Fragments arranged by size: 3014 1909 1156 ApoI r'AATT_y Cuts at: 1493 3388 3614 3766 5875 1493 Size: 1895 226 152 2109 1697 Fragments arranged by size: 2109 1895 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4633 160 Size: 872 3601 1606 Fragments arranged by size: 3601 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 AvaI C'yCGr_G Cuts at: 1870 1943 2765 3404 1870 Size: 73 822 639 4545 Fragments arranged by size: 4545 822 639 73 BamHI G'GATC_C Cuts at: 3409 3776 3409 Size: 367 5712 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3400 4855 618 Size: 325 217 627 1613 1455 1842 Fragments arranged by size: 1842 1613 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2250 3398 1391 Size: 859 1148 4072 Fragments arranged by size: 4072 1148 859 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2466 928 Size: 971 567 4541 Fragments arranged by size: 4541 971 567 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4270 4304 1903 Size: 34 2333 34 3678 Fragments arranged by size: 3678 2333 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3971 5181 3971 Size: 1210 4869 BclI T'GATC_A Cuts at: 1548 1548 Size: 6079 BfrBI ATG'CAT Cuts at: 2806 2806 Size: 6079 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4690 136 Size: 107 122 71 631 605 3018 1525 Fragments arranged by size: 3018 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6074 6074 Size: 6079 BlpI GC'TnA_GC Cuts at: 2093 2249 3496 2093 Size: 156 1247 4676 Fragments arranged by size: 4676 1247 156 Bme1580I G_kGCm'C Cuts at: 1790 2231 2766 4140 5296 1790 Size: 441 535 1374 1156 2573 Fragments arranged by size: 2573 1374 1156 535 441 BmgBI CAC'GTC Cuts at: 1318 2872 3235 1318 Size: 1554 363 4162 Fragments arranged by size: 4162 1554 363 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3041 3810 4768 Size: 416 53 83 399 24 1624 769 958 Cuts at: 4768 442 Size: 1753 Fragments arranged by size: 1753 1624 958 769 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6079 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2086 2245 2286 2366 2765 2798 2897 4739 Size: 847 159 41 80 399 33 99 1842 Cuts at: 4739 1239 Size: 2579 Fragments arranged by size: 2579 1842 847 399 159 99 80 41 33 Bpu10I CC'TnA_GC Cuts at: 2186 3064 2186 Size: 878 5201 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2387 4204 5069 5367 2387 Size: 1817 865 298 3099 Fragments arranged by size: 3099 1817 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2062 2660 2741 3266 4742 882 Size: 617 563 598 81 525 1476 2219 Fragments arranged by size: 2219 1476 617 598 563 525 81 BsaAI yAC'GTr Cuts at: 493 1204 2905 3208 3870 493 Size: 711 1701 303 662 2702 Fragments arranged by size: 2702 1701 711 662 303 BsaBI GATnn'nnATC Cuts at: 6073 6073 Size: 6079 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3886 4268 4903 275 Size: 53 83 186 936 2353 382 635 1451 Fragments arranged by size: 2353 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2197 4512 4910 5184 5331 5742 2197 Size: 2315 398 274 147 411 2534 Fragments arranged by size: 2534 2315 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2138 2168 3176 3206 1178 Size: 30 165 30 735 30 1008 30 4051 Fragments arranged by size: 4051 1008 735 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2162 2678 2795 1186 Size: 976 516 117 4470 Fragments arranged by size: 4470 976 516 117 BseYI C'CCAG_C Cuts at: 2104 2134 2896 5282 5778 2104 Size: 30 762 2386 496 2405 Fragments arranged by size: 2405 2386 762 496 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3026 1163 Size: 573 1290 4216 Fragments arranged by size: 4216 1290 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2548 3529 4290 5318 1540 Size: 275 186 547 981 761 1028 2301 Fragments arranged by size: 2301 1028 981 761 547 275 186 BsiHKAI G_wGCw'C Cuts at: 2169 2231 2250 4140 4225 5296 2169 Size: 62 19 1890 85 1071 2952 Fragments arranged by size: 2952 1890 1071 85 62 19 BsmI GAATG_Cn' Cuts at: 3590 3683 3590 Size: 93 5986 BsmBI CGTCTCn'nnnn_ Cuts at: 3353 3353 Size: 6079 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2169 2231 2250 2766 3398 4140 4225 Size: 399 379 62 19 516 632 742 85 Cuts at: 4225 5296 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 632 516 399 379 85 62 19 BspEI T'CCGG_A Cuts at: 5742 5742 Size: 6079 BspHI T'CATG_A Cuts at: 3132 3968 5698 3132 Size: 836 1730 3513 Fragments arranged by size: 3513 1730 836 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2190 2916 3048 3434 844 Size: 1346 726 132 386 3489 Fragments arranged by size: 3489 1346 726 386 132 BsrBI CCG'CTC Cuts at: 1394 1823 3966 1394 Size: 429 2143 3507 Fragments arranged by size: 3507 2143 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4574 4748 65 Size: 1183 3326 174 1396 Fragments arranged by size: 3326 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2921 4723 4910 1700 Size: 110 1111 1802 187 2869 Fragments arranged by size: 2869 1802 1111 187 110 BsrGI T'GTAC_A Cuts at: 96 2490 3007 96 Size: 2394 517 3168 Fragments arranged by size: 3168 2394 517 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6079 BssSI C'ACGA_G Cuts at: 4139 5151 4139 Size: 1012 5067 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6079 BstBI TT'CG_AA Cuts at: 4883 4883 Size: 6079 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6079 BstYI r'GATC_y Cuts at: 1972 2880 3409 3776 4161 4178 5630 5716 5728 Size: 908 529 367 385 17 1452 86 12 Cuts at: 5728 6074 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 908 529 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2241 2913 513 Size: 552 1176 672 3679 Fragments arranged by size: 3679 1176 672 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3241 487 Size: 683 56 2015 3325 Fragments arranged by size: 3325 2015 683 56 BtsI GCAGTG_nn' Cuts at: 2159 2552 3320 3595 4389 4409 2159 Size: 393 768 275 794 20 3829 Fragments arranged by size: 3829 794 768 393 275 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6079 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6044 DraI TTT'AAA Cuts at: 3383 3383 Size: 6079 DraIII CAC_nnn'GTG Cuts at: 2226 2695 3049 2226 Size: 469 354 5256 Fragments arranged by size: 5256 469 354 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5086 809 Size: 507 3770 1802 Fragments arranged by size: 3770 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2283 2697 3526 Size: 54 1425 50 275 18 453 414 829 Cuts at: 3526 4415 6044 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 829 453 414 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3526 1537 Size: 275 1714 4090 Fragments arranged by size: 4090 1714 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2393 2562 2765 4009 1353 Size: 1040 169 203 1244 3423 Fragments arranged by size: 3423 1244 1040 203 169 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2495 4666 5040 5186 387 Size: 1064 138 906 2171 374 146 1280 Fragments arranged by size: 2171 1280 1064 906 374 146 138 EcoICRI GAG'CTC Cuts at: 2248 2248 Size: 6079 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2010 2086 2245 2286 2366 2765 2798 Size: 393 378 76 159 41 80 399 33 Cuts at: 2798 2897 4142 4739 5526 5794 1239 Size: 99 1245 597 787 268 1524 Fragments arranged by size: 1524 1245 787 597 399 393 378 268 159 99 80 76 41 33 EcoNI CCTnn'n_nnAGG Cuts at: 2336 3149 2336 Size: 813 5266 EcoO109I rG'GnC_Cy Cuts at: 2109 2341 2944 3117 2109 Size: 232 603 173 5071 Fragments arranged by size: 5071 603 232 173 EcoRI G'AATT_C Cuts at: 3388 3766 3388 Size: 378 5701 FspI TGC'GCA Cuts at: 2651 4585 2651 Size: 1934 4145 HaeII r_GCGC'y Cuts at: 1678 2359 5226 1678 Size: 681 2867 2531 Fragments arranged by size: 2867 2531 681 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2542 2574 2946 2978 3109 Size: 32 135 32 970 32 372 32 131 Cuts at: 3109 3141 3176 3208 4720 4752 4794 4826 1373 Size: 32 35 32 1512 32 42 32 2626 Fragments arranged by size: 2626 1512 970 372 135 131 42 35 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2539 3423 3669 669 Size: 988 281 601 884 246 3079 Fragments arranged by size: 3079 988 884 601 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6079 HpaI GTT'AAC Cuts at: 3669 3669 Size: 6079 KpnI G_GTAC'C Cuts at: 3404 3404 Size: 6079 MfeI C'AATT_G Cuts at: 3678 3678 Size: 6079 MluI A'CGCG_T Cuts at: 5955 5955 Size: 6079 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2295 2932 3192 3219 5193 5377 1305 Size: 990 637 260 27 1974 184 2007 Fragments arranged by size: 2007 1974 990 637 260 184 27 MscI TGG'CCA Cuts at: 10 64 1489 1832 2285 2699 10 Size: 54 1425 343 453 414 3390 Fragments arranged by size: 3390 1425 453 414 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2018 2801 3146 4037 4396 4555 518 Size: 648 852 783 345 891 359 159 2042 Fragments arranged by size: 2042 891 852 783 648 359 345 159 MspA1I CmG'CkG Cuts at: 1606 2134 2306 3190 3495 4172 5320 5565 1606 Size: 528 172 884 305 677 1148 245 2120 Fragments arranged by size: 2120 1148 884 677 528 305 245 172 NaeI GCC'GGC Cuts at: 2923 2923 Size: 6079 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5527 NdeI CA'TA_TG Cuts at: 387 387 Size: 6079 NgoMIV G'CCGG_C Cuts at: 2921 2921 Size: 6079 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6079 NotI GC'GGCC_GC Cuts at: 3526 3526 Size: 6079 NsiI A_TGCA'T Cuts at: 2808 2808 Size: 6079 NspI r_CATG'y Cuts at: 1132 1229 2216 2806 2810 3437 4982 1132 Size: 97 987 590 4 627 1545 2229 Fragments arranged by size: 2229 1545 987 627 590 97 4 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6079 PciI A'CATG_T Cuts at: 4978 4978 Size: 6079 PflMI CCAn_nnn'nTGG Cuts at: 1108 2344 2695 3211 1108 Size: 1236 351 516 3976 Fragments arranged by size: 3976 1236 516 351 PfoI T'CCnGG_A Cuts at: 2742 2742 Size: 6079 PmeI GTTT'AAAC Cuts at: 3383 3383 Size: 6079 PmlI CAC'GTG Cuts at: 3208 3208 Size: 6079 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4148 4180 5661 5693 4148 Size: 32 1481 32 4534 Fragments arranged by size: 4534 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2109 2944 3117 2109 Size: 835 173 5071 Fragments arranged by size: 5071 835 173 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6079 PsiI TTA'TAA Cuts at: 3075 3649 3075 Size: 574 5505 PstI C_TGCA'G Cuts at: 830 3431 830 Size: 2601 3478 PvuI CG_AT'CG Cuts at: 2001 2548 2001 Size: 547 5532 RsrII CG'GwC_CG Cuts at: 3192 3192 Size: 6079 SacI G_AGCT'C Cuts at: 2250 2250 Size: 6079 SalI G'TCGA_C Cuts at: 1936 3421 1936 Size: 1485 4594 SanDI GG'GwC_CC Cuts at: 2109 2109 Size: 6079 SapI GCTCTTCn'nnn_ Cuts at: 2393 2765 2393 Size: 372 5707 SbfI CC_TGCA'GG Cuts at: 3431 3431 Size: 6079 ScaI AGT'ACT Cuts at: 3311 4327 6039 3311 Size: 1016 1712 3351 Fragments arranged by size: 3351 1712 1016 SfcI C'TryA_G Cuts at: 826 1045 3427 4562 5243 5434 826 Size: 219 2382 1135 681 191 1471 Fragments arranged by size: 2382 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6079 SmaI CCC'GGG Cuts at: 1872 2767 3406 1872 Size: 895 639 4545 Fragments arranged by size: 4545 895 639 SmlI C'TyrA_G Cuts at: 820 1017 1943 2402 4183 5084 5346 820 Size: 197 926 459 1781 901 262 1553 Fragments arranged by size: 1781 1553 926 901 459 262 197 SnaBI TAC'GTA Cuts at: 493 3870 493 Size: 3377 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6079 SphI G_CATG'C Cuts at: 1132 2216 2806 2810 3437 1132 Size: 1084 590 4 627 3774 Fragments arranged by size: 3774 1084 627 590 4 SspI AAT'ATT Cuts at: 5 52 4003 5 Size: 47 3951 2081 Fragments arranged by size: 3951 2081 47 StuI AGG'CCT Cuts at: 1517 2175 2297 3241 1517 Size: 658 122 944 4355 Fragments arranged by size: 4355 944 658 122 StyI C'CwwG_G Cuts at: 513 1065 2292 2631 3518 513 Size: 552 1227 339 887 3074 Fragments arranged by size: 3074 1227 887 552 339 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2050 2071 3060 4106 4123 4276 4461 Size: 1255 470 21 989 1046 17 153 185 Cuts at: 4461 4906 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1046 989 470 445 185 153 21 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2490 2820 3007 Size: 274 80 33 51 1443 513 330 187 Cuts at: 3007 3266 3309 4325 6037 96 Size: 259 43 1016 1712 138 Fragments arranged by size: 1712 1443 1016 513 330 274 259 187 138 80 51 43 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2138 2170 6039 6071 2138 Size: 32 3869 32 2146 Fragments arranged by size: 3869 2146 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6079 XbaI T'CTAG_A Cuts at: 3415 3415 Size: 6079 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6079 XmaI C'CCGG_G Cuts at: 1870 2765 3404 1870 Size: 895 639 4545 Fragments arranged by size: 4545 895 639 XmnI GAAnn'nnTTC Cuts at: 4208 4208 Size: 6079 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3887 276 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NarI NruI PacI PspOMI PspXI PsrI PvuII SacII SexAI SfiI SfoI SrfI SwaI XcmI