(Circular) (Six-base) MAPSORT of: pFN21AB7038.seq Check: 1824 from: 1 to: 6076 pFN21AB7038 6076 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3057 3057 Size: 6076 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3886 278 Size: 53 83 186 936 2350 2468 Fragments arranged by size: 2468 2350 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3419 1937 Size: 1482 4594 Acc65I G'GTAC_C Cuts at: 3397 3397 Size: 6076 AclI AA'CG_TT Cuts at: 1563 4203 4576 1563 Size: 2640 373 3063 Fragments arranged by size: 3063 2640 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2678 2813 2990 3116 4139 5523 5791 1632 Size: 1046 135 177 126 1023 1384 268 1917 Fragments arranged by size: 1917 1384 1046 1023 268 177 135 126 AfeI AGC'GCT Cuts at: 2609 2765 2609 Size: 156 5920 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5879 AflIII A'CryG_T Cuts at: 1203 2127 2196 2727 4975 5952 1203 Size: 924 69 531 2248 977 1327 Fragments arranged by size: 2248 1327 977 924 531 69 AgeI A'CCGG_T Cuts at: 4907 4907 Size: 6076 AhdI GACnn_n'nnGTC Cuts at: 4805 4805 Size: 6076 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6076 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6076 AlwNI CAG_nnn'CTG Cuts at: 3819 5391 3819 Size: 1572 4504 ApaI G_GGCC'C Cuts at: 3059 3281 3059 Size: 222 5854 ApaLI G'TGCA_C Cuts at: 4133 5289 4133 Size: 1156 4920 ApoI r'AATT_y Cuts at: 1493 3385 3611 3763 5872 1493 Size: 1892 226 152 2109 1697 Fragments arranged by size: 2109 1892 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4630 160 Size: 872 3598 1606 Fragments arranged by size: 3598 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6076 AvaI C'yCGr_G Cuts at: 1870 1943 2069 2167 2342 2719 2864 2920 3020 Size: 73 126 98 175 377 145 56 100 Cuts at: 3020 3070 3401 1870 Size: 50 331 4545 Fragments arranged by size: 4545 377 331 175 145 126 100 98 73 56 50 BamHI G'GATC_C Cuts at: 3406 3773 3406 Size: 367 5709 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2258 2662 2807 2903 3010 Size: 325 217 627 471 404 145 96 107 Cuts at: 3010 3397 4852 618 Size: 387 1455 1842 Fragments arranged by size: 1842 1455 627 471 404 387 325 217 145 107 96 BanII G_rGCy'C Cuts at: 1391 2070 2189 2228 2339 2408 2531 2696 2845 Size: 679 119 39 111 69 123 165 149 Cuts at: 2845 2999 3059 3281 3395 1391 Size: 154 60 222 114 4072 Fragments arranged by size: 4072 679 222 165 154 149 123 119 114 111 69 60 39 BbeI G_GCGC'C Cuts at: 2262 2811 3014 2262 Size: 549 203 5324 Fragments arranged by size: 5324 549 203 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5105 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2713 2747 4267 4301 1903 Size: 34 50 34 692 34 1520 34 3678 Fragments arranged by size: 3678 1520 692 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3968 5178 3968 Size: 1210 4866 BclI T'GATC_A Cuts at: 1548 1548 Size: 6076 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2291 2300 2855 Size: 107 122 71 631 605 619 9 555 Cuts at: 2855 2882 4687 136 Size: 27 1805 1525 Fragments arranged by size: 1805 1525 631 619 605 555 122 107 71 27 9 BglII A'GATC_T Cuts at: 2556 6071 2556 Size: 3515 2561 BlpI GC'TnA_GC Cuts at: 2549 2993 3493 2549 Size: 444 500 5132 Fragments arranged by size: 5132 500 444 Bme1580I G_kGCm'C Cuts at: 1790 2034 2667 2906 3059 3281 4137 5293 1790 Size: 244 633 239 153 222 856 1156 2573 Fragments arranged by size: 2573 1156 856 633 244 239 222 153 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6076 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3807 4765 442 Size: 416 53 83 399 24 2390 958 1753 Fragments arranged by size: 2390 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6076 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2095 2095 Size: 6076 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2068 4736 1239 Size: 829 2668 2579 Fragments arranged by size: 2668 2579 829 Bpu10I CC'TnA_GC Cuts at: 2333 2429 2333 Size: 96 5980 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2341 3194 4201 5066 5364 2341 Size: 853 1007 865 298 3053 Fragments arranged by size: 3053 1007 865 853 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4739 882 Size: 617 3240 2219 Fragments arranged by size: 3240 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2130 2209 3867 493 Size: 711 926 79 1658 2702 Fragments arranged by size: 2702 1658 926 711 79 BsaBI GATnn'nnATC Cuts at: 6070 6070 Size: 6076 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2259 2808 2978 3011 Size: 53 83 186 936 726 549 170 33 Cuts at: 3011 3306 3883 4265 4900 275 Size: 295 577 382 635 1451 Fragments arranged by size: 1451 936 726 635 577 549 382 295 186 170 83 53 33 BsaWI w'CCGG_w Cuts at: 4509 4907 5181 5328 5739 4509 Size: 398 274 147 411 4846 Fragments arranged by size: 4846 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2381 2411 2621 2651 3116 Size: 30 165 30 978 30 210 30 465 Cuts at: 3116 3146 1178 Size: 30 4108 Fragments arranged by size: 4108 978 465 210 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2071 3361 1186 Size: 885 1290 3901 Fragments arranged by size: 3901 1290 885 BseYI C'CCAG_C Cuts at: 2008 2327 3052 5279 5775 2008 Size: 319 725 2227 496 2309 Fragments arranged by size: 2309 2227 725 496 319 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2693 1163 Size: 573 957 4546 Fragments arranged by size: 4546 957 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2151 2286 2295 2358 2835 3526 Size: 275 186 150 135 9 63 477 691 Cuts at: 3526 4287 5315 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 691 477 275 186 150 135 63 9 BsiHKAI G_wGCw'C Cuts at: 2946 4137 4222 5293 2946 Size: 1191 85 1071 3729 Fragments arranged by size: 3729 1191 1071 85 BsiWI C'GTAC_G Cuts at: 2205 2205 Size: 6076 BsmI GAATG_Cn' Cuts at: 3587 3680 3587 Size: 93 5983 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2070 2189 2228 2339 2408 2531 Size: 399 244 36 119 39 111 69 123 Cuts at: 2531 2667 2696 2845 2906 2946 2999 3059 3281 Size: 136 29 149 61 40 53 60 222 Cuts at: 3281 3395 4137 4222 5293 1391 Size: 114 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 244 222 149 136 123 119 114 111 85 69 61 60 53 40 39 36 29 BspEI T'CCGG_A Cuts at: 5739 5739 Size: 6076 BspHI T'CATG_A Cuts at: 3965 5695 3965 Size: 1730 4346 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2757 3057 3431 844 Size: 1913 300 374 3489 Fragments arranged by size: 3489 1913 374 300 BsrBI CCG'CTC Cuts at: 1394 1823 2015 2341 2670 3963 1394 Size: 429 192 326 329 1293 3507 Fragments arranged by size: 3507 1293 429 329 326 192 BsrDI GCAATG_nn' Cuts at: 65 1248 4571 4745 65 Size: 1183 3323 174 1396 Fragments arranged by size: 3323 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2102 2294 2592 2813 2881 2980 3007 Size: 110 292 192 298 221 68 99 27 Cuts at: 3007 4720 4907 1700 Size: 1713 187 2869 Fragments arranged by size: 2869 1713 298 292 221 192 187 110 99 68 27 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6076 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6076 BssSI C'ACGA_G Cuts at: 2033 4136 5148 2033 Size: 2103 1012 2961 Fragments arranged by size: 2961 2103 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6076 BstBI TT'CG_AA Cuts at: 4880 4880 Size: 6076 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6076 BstYI r'GATC_y Cuts at: 1972 2556 3406 3773 4158 4175 5627 5713 5725 Size: 584 850 367 385 17 1452 86 12 Cuts at: 5725 6071 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 850 584 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3084 3084 Size: 6076 BtgI C'CryG_G Cuts at: 513 1065 2154 2262 2364 2511 3039 3273 513 Size: 552 1089 108 102 147 528 234 3316 Fragments arranged by size: 3316 1089 552 528 234 147 108 102 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5337 Fragments arranged by size: 5337 683 56 BtsI GCAGTG_nn' Cuts at: 3592 4386 4406 3592 Size: 794 20 5262 Fragments arranged by size: 5262 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6076 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6041 DraI TTT'AAA Cuts at: 3380 3380 Size: 6076 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5083 809 Size: 507 3767 1802 Fragments arranged by size: 3767 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2148 2283 2292 Size: 54 1425 50 275 18 318 135 9 Cuts at: 2292 2355 2514 2832 3270 3318 3523 4412 6041 Size: 63 159 318 438 48 205 889 1629 Cuts at: 6041 8 Size: 43 Fragments arranged by size: 1629 1425 889 438 318 318 275 205 159 135 63 54 50 48 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2148 2283 2292 2355 2832 3523 1537 Size: 275 336 135 9 63 477 691 4090 Fragments arranged by size: 4090 691 477 336 275 135 63 9 EarI CTCTTCn'nnn_ Cuts at: 1353 2585 4006 1353 Size: 1232 1421 3423 Fragments arranged by size: 3423 1421 1232 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2615 4663 5037 5183 387 Size: 1064 138 1026 2048 374 146 1280 Fragments arranged by size: 2048 1280 1064 1026 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2068 2678 2813 2990 3116 4139 4736 Size: 393 436 610 135 177 126 1023 597 Cuts at: 4736 5523 5791 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1023 787 610 597 436 393 268 177 135 126 EcoO109I rG'GnC_Cy Cuts at: 2956 2956 Size: 6076 EcoRI G'AATT_C Cuts at: 3385 3763 3385 Size: 378 5698 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2713 2713 Size: 6076 FseI GG_CCGG'CC Cuts at: 2885 2885 Size: 6076 FspI TGC'GCA Cuts at: 4582 4582 Size: 6076 HaeII r_GCGC'y Cuts at: 1678 2217 2262 2611 2767 2792 2811 2928 2993 Size: 539 45 349 156 25 19 117 65 Cuts at: 2993 3014 5223 1678 Size: 21 2209 2531 Fragments arranged by size: 2531 2209 539 349 156 117 65 45 25 21 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4717 4749 4791 4823 1373 Size: 32 135 32 3145 32 42 32 2626 Fragments arranged by size: 3145 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3420 3666 669 Size: 988 281 1482 246 3079 Fragments arranged by size: 3079 1482 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6076 HpaI GTT'AAC Cuts at: 3666 3666 Size: 6076 KasI G'GCGC_C Cuts at: 2258 2807 3010 2258 Size: 549 203 5324 Fragments arranged by size: 5324 549 203 KpnI G_GTAC'C Cuts at: 3401 3401 Size: 6076 MfeI C'AATT_G Cuts at: 3675 3675 Size: 6076 MluI A'CGCG_T Cuts at: 5952 5952 Size: 6076 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2067 5190 5374 1305 Size: 762 3123 184 2007 Fragments arranged by size: 3123 2007 762 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2516 3272 10 Size: 54 1425 343 684 756 2814 Fragments arranged by size: 2814 1425 756 684 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2132 2138 2195 2510 4034 4393 4552 Size: 648 966 6 57 315 1524 359 159 Cuts at: 4552 518 Size: 2042 Fragments arranged by size: 2042 1524 966 648 359 315 159 57 6 MspA1I CmG'CkG Cuts at: 1606 2163 2785 2820 3041 3492 4169 5317 5562 Size: 557 622 35 221 451 677 1148 245 Cuts at: 5562 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 622 557 451 245 221 35 NaeI GCC'GGC Cuts at: 2104 2296 2815 2883 2982 3009 2104 Size: 192 519 68 99 27 5171 Fragments arranged by size: 5171 519 192 99 68 27 NarI GG'CG_CC Cuts at: 2259 2808 3011 2259 Size: 549 203 5324 Fragments arranged by size: 5324 549 203 NcoI C'CATG_G Cuts at: 513 1065 2154 2262 2364 2511 3273 513 Size: 552 1089 108 102 147 762 3316 Fragments arranged by size: 3316 1089 762 552 147 108 102 NdeI CA'TA_TG Cuts at: 387 387 Size: 6076 NgoMIV G'CCGG_C Cuts at: 2102 2294 2813 2881 2980 3007 2102 Size: 192 519 68 99 27 5171 Fragments arranged by size: 5171 519 192 99 68 27 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6076 NotI GC'GGCC_GC Cuts at: 2148 2832 3523 2148 Size: 684 691 4701 Fragments arranged by size: 4701 691 684 NspI r_CATG'y Cuts at: 1132 1229 2143 2200 2731 3434 4979 1132 Size: 97 914 57 531 703 1545 2229 Fragments arranged by size: 2229 1545 914 703 531 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6076 PciI A'CATG_T Cuts at: 2196 2727 4975 2196 Size: 531 2248 3297 Fragments arranged by size: 3297 2248 531 PflMI CCAn_nnn'nTGG Cuts at: 1108 3052 3202 1108 Size: 1944 150 3982 Fragments arranged by size: 3982 1944 150 PmeI GTTT'AAAC Cuts at: 3380 3380 Size: 6076 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4145 4177 5658 5690 4145 Size: 32 1481 32 4531 Fragments arranged by size: 4531 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6076 PsiI TTA'TAA Cuts at: 3646 3646 Size: 6076 PspOMI G'GGCC_C Cuts at: 3055 3277 3055 Size: 222 5854 PspXI vC'TCGA_Gb Cuts at: 2920 2920 Size: 6076 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2729 2761 2729 Size: 32 6044 PstI C_TGCA'G Cuts at: 830 3428 830 Size: 2598 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6076 PvuII CAG'CTG Cuts at: 2785 2785 Size: 6076 SacII CC_GC'GG Cuts at: 3042 3042 Size: 6076 SalI G'TCGA_C Cuts at: 1936 3418 1936 Size: 1482 4594 SbfI CC_TGCA'GG Cuts at: 3428 3428 Size: 6076 ScaI AGT'ACT Cuts at: 4324 6036 4324 Size: 1712 4364 SfcI C'TryA_G Cuts at: 826 1045 2455 3424 4559 5240 5431 826 Size: 219 1410 969 1135 681 191 1471 Fragments arranged by size: 1471 1410 1135 969 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2291 2291 Size: 6076 SfoI GGC'GCC Cuts at: 2260 2809 3012 2260 Size: 549 203 5324 Fragments arranged by size: 5324 549 203 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6076 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6076 SmaI CCC'GGG Cuts at: 1872 3022 3072 3403 1872 Size: 1150 50 331 4545 Fragments arranged by size: 4545 1150 331 50 SmlI C'TyrA_G Cuts at: 820 1017 1943 2320 2920 3209 4180 5081 5343 Size: 197 926 377 600 289 971 901 262 Cuts at: 5343 820 Size: 1553 Fragments arranged by size: 1553 971 926 901 600 377 289 262 197 SnaBI TAC'GTA Cuts at: 493 3867 493 Size: 3374 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6076 SphI G_CATG'C Cuts at: 1132 3434 1132 Size: 2302 3774 SspI AAT'ATT Cuts at: 5 52 4000 5 Size: 47 3948 2081 Fragments arranged by size: 3948 2081 47 StuI AGG'CCT Cuts at: 1517 2849 3215 1517 Size: 1332 366 4378 Fragments arranged by size: 4378 1332 366 StyI C'CwwG_G Cuts at: 513 1065 2154 2262 2364 2511 3273 3515 513 Size: 552 1089 108 102 147 762 242 3074 Fragments arranged by size: 3074 1089 762 552 242 147 108 102 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4103 4120 4273 4458 4903 325 Size: 1255 2523 17 153 185 445 1498 Fragments arranged by size: 2523 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3348 4322 6034 Size: 274 80 33 51 1443 1371 974 1712 Cuts at: 6034 96 Size: 138 Fragments arranged by size: 1712 1443 1371 974 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2024 2056 2621 2653 3116 3148 6036 6068 2024 Size: 32 565 32 463 32 2888 32 2032 Fragments arranged by size: 2888 2032 565 463 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6076 XbaI T'CTAG_A Cuts at: 3412 3412 Size: 6076 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2269 2371 2269 Size: 102 5974 XhoI C'TCGA_G Cuts at: 1943 2920 1943 Size: 977 5099 XmaI C'CCGG_G Cuts at: 1870 3020 3070 3401 1870 Size: 1150 50 331 4545 Fragments arranged by size: 4545 1150 331 50 XmnI GAAnn'nnTTC Cuts at: 2765 4205 2765 Size: 1440 4636 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3884 276 Size: 53 83 186 936 2350 2468 Fragments arranged by size: 2468 2350 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FspAI NruI NsiI PacI PfoI PmlI PpuMI RsrII SacI SanDI SapI SexAI SrfI SwaI