Restriction map for
pFN21AB7038
(Linear) (Six-base) MAPSORT of: KIBB7038.seq Check: 7238 from: 1 to: 1371
KIBB7038.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1052 1371
Size: 1052 319
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 808 985 1111 1371
Size: 673 135 177 126 260
Fragments arranged by size:
673 260 177 135 126
AfeI AGC'GCT
Cuts at: 0 604 760 1371
Size: 604 156 611
Fragments arranged by size:
611 604 156
AflIII A'CryG_T
Cuts at: 0 122 191 722 1371
Size: 122 69 531 649
Fragments arranged by size:
649 531 122 69
ApaI G_GGCC'C
Cuts at: 0 1054 1276 1371
Size: 1054 222 95
Fragments arranged by size:
1054 222 95
AvaI C'yCGr_G
Cuts at: 0 64 162 337 714 859 915 1015 1065
Size: 64 98 175 377 145 56 100 50
Cuts at: 1065 1371
Size: 306
Fragments arranged by size:
377 306 175 145 100 98 64 56
50
BanI G'GyrC_C
Cuts at: 0 253 657 802 898 1005 1371
Size: 253 404 145 96 107 366
Fragments arranged by size:
404 366 253 145 107 96
BanII G_rGCy'C
Cuts at: 0 65 184 223 334 403 526 691 840
Size: 65 119 39 111 69 123 165 149
Cuts at: 840 994 1054 1276 1371
Size: 154 60 222 95
Fragments arranged by size:
222 165 154 149 123 119 111 95
69 65 60 39
BbeI G_GCGC'C
Cuts at: 0 257 806 1009 1371
Size: 257 549 203 362
Fragments arranged by size:
549 362 257 203
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 708 742 1371
Size: 708 34 629
Fragments arranged by size:
708 629 34
BglI GCCn_nnn'nGGC
Cuts at: 0 286 295 850 877 1371
Size: 286 9 555 27 494
Fragments arranged by size:
555 494 286 27 9
BglII A'GATC_T
Cuts at: 0 551 1371
Size: 551 820
BlpI GC'TnA_GC
Cuts at: 0 544 988 1371
Size: 544 444 383
Fragments arranged by size:
544 444 383
Bme1580I G_kGCm'C
Cuts at: 0 29 662 901 1054 1276 1371
Size: 29 633 239 153 222 95
Fragments arranged by size:
633 239 222 153 95 29
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 90 1371
Size: 90 1281
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 1371
Size: 63 1308
Bpu10I CC'TnA_GC
Cuts at: 0 328 424 1371
Size: 328 96 947
Fragments arranged by size:
947 328 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 1189 1371
Size: 336 853 182
Fragments arranged by size:
853 336 182
BsaAI yAC'GTr
Cuts at: 0 125 204 1371
Size: 125 79 1167
Fragments arranged by size:
1167 125 79
BsaHI Gr'CG_yC
Cuts at: 0 254 803 973 1006 1301 1371
Size: 254 549 170 33 295 70
Fragments arranged by size:
549 295 254 170 70 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 376 406 616 646 1111 1141 1371
Size: 376 30 210 30 465 30 230
Fragments arranged by size:
465 376 230 210 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 66 1356 1371
Size: 66 1290 15
Fragments arranged by size:
1290 66 15
BseYI C'CCAG_C
Cuts at: 0 3 322 1047 1371
Size: 3 319 725 324
Fragments arranged by size:
725 324 319 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 1371
Size: 688 683
BsiEI CG_ry'CG
Cuts at: 0 146 281 290 353 830 1371
Size: 146 135 9 63 477 541
Fragments arranged by size:
541 477 146 135 63 9
BsiHKAI G_wGCw'C
Cuts at: 0 941 1371
Size: 941 430
BsiWI C'GTAC_G
Cuts at: 0 200 1371
Size: 200 1171
Bsp1286I G_dGCh'C
Cuts at: 0 29 65 184 223 334 403 526 662
Size: 29 36 119 39 111 69 123 136
Cuts at: 662 691 840 901 941 994 1054 1276 1371
Size: 29 149 61 40 53 60 222 95
Fragments arranged by size:
222 149 136 123 119 111 95 69
61 60 53 40 39 36 29 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 752 1052 1371
Size: 752 300 319
Fragments arranged by size:
752 319 300
BsrBI CCG'CTC
Cuts at: 0 10 336 665 1371
Size: 10 326 329 706
Fragments arranged by size:
706 329 326 10
BsrFI r'CCGG_y
Cuts at: 0 97 289 587 808 876 975 1002 1371
Size: 97 192 298 221 68 99 27 369
Fragments arranged by size:
369 298 221 192 99 97 68 27
BssSI C'ACGA_G
Cuts at: 0 28 1371
Size: 28 1343
BstYI r'GATC_y
Cuts at: 0 551 1371
Size: 551 820
Bsu36I CC'TnA_GG
Cuts at: 0 1079 1371
Size: 1079 292
BtgI C'CryG_G
Cuts at: 0 149 257 359 506 1034 1268 1371
Size: 149 108 102 147 528 234 103
Fragments arranged by size:
528 234 149 147 108 103 102
EaeI y'GGCC_r
Cuts at: 0 143 278 287 350 509 827 1265 1313
Size: 143 135 9 63 159 318 438 48
Cuts at: 1313 1371
Size: 58
Fragments arranged by size:
438 318 159 143 135 63 58 48
9
EagI C'GGCC_G
Cuts at: 0 143 278 287 350 827 1371
Size: 143 135 9 63 477 544
Fragments arranged by size:
544 477 143 135 63 9
EarI CTCTTCn'nnn_
Cuts at: 0 580 1371
Size: 580 791
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 610 1371
Size: 610 761
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 673 808 985 1111 1371
Size: 63 610 135 177 126 260
Fragments arranged by size:
610 260 177 135 126 63
EcoO109I rG'GnC_Cy
Cuts at: 0 951 1371
Size: 951 420
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 708 1371
Size: 708 663
FseI GG_CCGG'CC
Cuts at: 0 880 1371
Size: 880 491
HaeII r_GCGC'y
Cuts at: 0 212 257 606 762 787 806 923 988
Size: 212 45 349 156 25 19 117 65
Cuts at: 988 1009 1371
Size: 21 362
Fragments arranged by size:
362 349 212 156 117 65 45 25
21 19
KasI G'GCGC_C
Cuts at: 0 253 802 1005 1371
Size: 253 549 203 366
Fragments arranged by size:
549 366 253 203
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 1371
Size: 62 1309
MscI TGG'CCA
Cuts at: 0 511 1267 1371
Size: 511 756 104
Fragments arranged by size:
756 511 104
MslI CAynn'nnrTG
Cuts at: 0 127 133 190 505 1371
Size: 127 6 57 315 866
Fragments arranged by size:
866 315 127 57 6
MspA1I CmG'CkG
Cuts at: 0 158 780 815 1036 1371
Size: 158 622 35 221 335
Fragments arranged by size:
622 335 221 158 35
NaeI GCC'GGC
Cuts at: 0 99 291 810 878 977 1004 1371
Size: 99 192 519 68 99 27 367
Fragments arranged by size:
519 367 192 99 99 68 27
NarI GG'CG_CC
Cuts at: 0 254 803 1006 1371
Size: 254 549 203 365
Fragments arranged by size:
549 365 254 203
NcoI C'CATG_G
Cuts at: 0 149 257 359 506 1268 1371
Size: 149 108 102 147 762 103
Fragments arranged by size:
762 149 147 108 103 102
NgoMIV G'CCGG_C
Cuts at: 0 97 289 808 876 975 1002 1371
Size: 97 192 519 68 99 27 369
Fragments arranged by size:
519 369 192 99 97 68 27
NotI GC'GGCC_GC
Cuts at: 0 143 827 1371
Size: 143 684 544
Fragments arranged by size:
684 544 143
NspI r_CATG'y
Cuts at: 0 138 195 726 1371
Size: 138 57 531 645
Fragments arranged by size:
645 531 138 57
PciI A'CATG_T
Cuts at: 0 191 722 1371
Size: 191 531 649
Fragments arranged by size:
649 531 191
PflMI CCAn_nnn'nTGG
Cuts at: 0 1047 1197 1371
Size: 1047 150 174
Fragments arranged by size:
1047 174 150
PspOMI G'GGCC_C
Cuts at: 0 1050 1272 1371
Size: 1050 222 99
Fragments arranged by size:
1050 222 99
PspXI vC'TCGA_Gb
Cuts at: 0 915 1371
Size: 915 456
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 724 756 1371
Size: 724 32 615
Fragments arranged by size:
724 615 32
PvuII CAG'CTG
Cuts at: 0 780 1371
Size: 780 591
SacII CC_GC'GG
Cuts at: 0 1037 1371
Size: 1037 334
SfcI C'TryA_G
Cuts at: 0 450 1371
Size: 450 921
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 286 1371
Size: 286 1085
SfoI GGC'GCC
Cuts at: 0 255 804 1007 1371
Size: 255 549 203 364
Fragments arranged by size:
549 364 255 203
SmaI CCC'GGG
Cuts at: 0 1017 1067 1371
Size: 1017 50 304
Fragments arranged by size:
1017 304 50
SmlI C'TyrA_G
Cuts at: 0 315 915 1204 1371
Size: 315 600 289 167
Fragments arranged by size:
600 315 289 167
StuI AGG'CCT
Cuts at: 0 844 1210 1371
Size: 844 366 161
Fragments arranged by size:
844 366 161
StyI C'CwwG_G
Cuts at: 0 149 257 359 506 1268 1371
Size: 149 108 102 147 762 103
Fragments arranged by size:
762 149 147 108 103 102
TatI w'GTAC_w
Cuts at: 0 1343 1371
Size: 1343 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 19 51 616 648 1111 1143 1371
Size: 19 32 565 32 463 32 228
Fragments arranged by size:
565 463 228 32 32 32 19
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 264 366 1371
Size: 264 102 1005
Fragments arranged by size:
1005 264 102
XhoI C'TCGA_G
Cuts at: 0 915 1371
Size: 915 456
XmaI C'CCGG_G
Cuts at: 0 1015 1065 1371
Size: 1015 50 306
Fragments arranged by size:
1015 306 50
XmnI GAAnn'nnTTC
Cuts at: 0 760 1371
Size: 760 611
Enzymes that do cut:
AarI AcuI AfeI AflIII ApaI AvaI BanI BanII
BbeI BcgI BglI BglII BlpI Bme1580I BplI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI Bsp1286I BspMI BsrBI BsrFI BssSI
BstYI Bsu36I BtgI EaeI EagI EarI EciI Eco57MI
EcoO109I FalI FseI HaeII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PciI
PflMI PspOMI PspXI PsrI PvuII SacII SfcI SfiI
SfoI SmaI SmlI StuI StyI TatI TstI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI
BmgBI BmrI BmtI BsaI BsaBI BsaWI BsmI BsmBI
BspEI BspHI BsrDI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EcoICRI EcoNI EcoRI EcoRV FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI NdeI NheI
NruI NsiI PacI PasI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI
ZraI