Restriction map for
pFN21AB7033
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AocI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BamHI BbsI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BpuAI BsaBI BsaI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI DraI
DrdI Eam1105I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI SacII SalI SbfI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcsI 634 GAATTC [GA]^AATT[CT]
ApaI 1137 GGGCCC GGGCC^C
ApoI 634 GAATTC [GA]^AATT[CT]
AvaI 808 CCCGAG C^[CT]CG[GA]G
AviII 398 TGCGCA TGC^GCA
BbeI 1166 GGCGCC GGCGC^C
BbrPI 334 CACGTG CAC^GTG
BsiEI 464 CGGCCG CG[GA][CT]^CG
BsmAI 621 GAGAC GTCTC(1/5)
BsmBI 621 GAGACG CGTCTC(1/5)
BsoBI 808 CCCGAG C^[CT]CG[GA]G
BspHI 1067 TCATGA T^CATGA
BsrDI 1101 CATTGC GCAATG(2/0)
BssHII 891 GCGCGC G^CGCGC
BssSI 1075 CACGAG CACGAG(-5/-1)
CpoI 548 CGGACCG CG^G[AT]CCG
EagI 464 CGGCCG C^GGCCG
EarI 182 CTCTTC CTCTTC(1/4)
EclXI 464 CGGCCG C^GGCCG
Eco52I 464 CGGCCG C^GGCCG
EcoRI 634 GAATTC G^AATTC
EcoT14I 425 CCAAGG C^C[AT][AT]GG
FspI 398 TGCGCA TGC^GCA
HincII 1045 GTCAAC GT[CT]^[GA]AC
HindII 1045 GTCAAC GT[CT]^[GA]AC
KasI 1166 GGCGCC G^GCGCC
Ksp632I 182 CTCTTC CTCTTC(1/4)
NaeI 462 GCCGGC GCC^GGC
NarI 1166 GGCGCC GG^CGCC
NgoMIV 462 GCCGGC G^CCGGC
PmaCI 334 CACGTG CAC^GTG
PmlI 334 CACGTG CAC^GTG
RcaI 1067 TCATGA T^CATGA
RsrII 548 CGGACCG CG^G[AT]CCG
SacI 598 GAGCTC GAGCT^C
SapI 181 GCTCTTC GCTCTTC(1/4)
ScaI 839 AGTACT AGT^ACT
SfiI 114 GGCCTTATCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1166 GGCGCC GGC^GCC
StyI 425 CCAAGG C^C[AT][AT]GG
TaqI 929 TCGA T^CGA
TfiI 198 GATTC G^A[AT]TC
TthHB8I 929 TCGA T^CGA
XcmI 1148 CCACTGCCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 623 GACGCC G[GA]^CG[CT]C
1166 GGCGCC
AfaI 747 GTAC GT^AC
840 GTAC
AflIII 294 ACATGT A^C[GA][CT]GT
908 ACATGT
Alw44I 298 GTGCAC G^TGCAC
537 GTGCAC
AlwI 816 GATCC GGATC(4/5)
369 GGATC
Aor51HI 902 AGCGCT AGC^GCT
968 AGCGCT
ApaLI 298 GTGCAC G^TGCAC
537 GTGCAC
BanII 598 GAGCTC G[GA]GC[CT]^C
1137 GGGCCC
BfaI 451 CTAG C^TAG
964 CTAG
BsaHI 623 GACGCC G[GA]^CG[CT]C
1166 GGCGCC
BspLU11I 294 ACATGT A^CATGT
908 ACATGT
BsrBI 12 GAGCGG CCGCTC(-3/-3)
582 GAGCGG
BsrFI 462 GCCGGC [GA]^CCGG[CT]
466 GCCGGT
BstF5I 410 CATCC GGATG(2/0)
660 CATCC
BstYI 369 GGATCT [GA]^GATC[CT]
815 AGATCC
Cfr10I 462 GCCGGC [GA]^CCGG[CT]
466 GCCGGT
DraII 764 AGGCCCT [GA]G^G[ACGT]CC[CT]
1136 AGGGCCC
DsaI 65 CCACGG C^C[GA][CT]GG
77 CCACGG
Eco47III 902 AGCGCT AGC^GCT
968 AGCGCT
EcoO109I 764 AGGCCCT [GA]G^G[ACGT]CC[CT]
1136 AGGGCCC
FokI 410 CATCC GGATG(9/13)
660 CATCC
Hin1I 623 GACGCC G[GA]^CG[CT]C
1166 GGCGCC
MaeI 451 CTAG C^TAG
964 CTAG
MboII 183 TCTTC GAAGA(8/7)
923 TCTTC
MflI 369 GGATCT [GA]^GATC[CT]
815 AGATCC
NspI 294 ACATGT [GA]CATG^[CT]
908 ACATGT
PflMI 846 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1086 CCAGACGCTGG
PstI 898 CTGCAG CTGCA^G
1117 CTGCAG
RsaI 747 GTAC GT^AC
840 GTAC
SexAI 377 ACCTGGT A^CC[AT]GGT
941 ACCTGGT
SfaNI 1000 GATGC GCATC(5/9)
409 GCATC
SfcI 898 CTGCAG C^T[GA][CT]AG
1117 CTGCAG
StuI 113 AGGCCT AGG^CCT
713 AGGCCT
Tsp509I 635 AATT ^AATT
1207 AATT
Van91I 846 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1086 CCAGACGCTGG
XhoII 369 GGATCT [GA]^GATC[CT]
815 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
AvaII BalI BanI BbvI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrI BssKI BstAPI BstNI BstUI
BstXI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraIII EaeI EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PvuII
Sau3AI Sau96I ScrFI SmlI TseI
Tsp45I TspRI