(Circular) (Six-base) MAPSORT of: pFN21AB7033.seq Check: 8116 from: 1 to: 5977 pFN21AB7033 5977 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2254 2790 2254 Size: 536 5441 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3787 278 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3320 1937 Size: 1383 4594 Acc65I G'GTAC_C Cuts at: 3298 3298 Size: 5977 AclI AA'CG_TT Cuts at: 1563 3276 4104 4477 1563 Size: 1713 828 373 3063 Fragments arranged by size: 3063 1713 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4040 5424 5692 1632 Size: 2408 1384 268 1917 Fragments arranged by size: 2408 1917 1384 268 AfeI AGC'GCT Cuts at: 2909 2975 2909 Size: 66 5911 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5780 AflIII A'CryG_T Cuts at: 1203 2299 2913 4876 5853 1203 Size: 1096 614 1963 977 1327 Fragments arranged by size: 1963 1327 1096 977 614 AgeI A'CCGG_T Cuts at: 4808 4808 Size: 5977 AhdI GACnn_n'nnGTC Cuts at: 4706 4706 Size: 5977 AleI CACnn'nnGTG Cuts at: 1166 2075 2148 2891 2994 1166 Size: 909 73 743 103 4149 Fragments arranged by size: 4149 909 743 103 73 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2909 1184 Size: 1725 4252 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2372 2404 2372 Size: 32 5945 AlwNI CAG_nnn'CTG Cuts at: 2530 3097 3130 3185 3206 3720 5292 2530 Size: 567 33 55 21 514 1572 3215 Fragments arranged by size: 3215 1572 567 514 55 33 21 ApaI G_GGCC'C Cuts at: 3146 3146 Size: 5977 ApaLI G'TGCA_C Cuts at: 2303 2542 4034 5190 2303 Size: 239 1492 1156 3090 Fragments arranged by size: 3090 1492 1156 239 ApoI r'AATT_y Cuts at: 1493 2639 3286 3512 3664 5773 1493 Size: 1146 647 226 152 2109 1697 Fragments arranged by size: 2109 1697 1146 647 226 152 AseI AT'TA_AT Cuts at: 160 1032 4531 160 Size: 872 3499 1606 Fragments arranged by size: 3499 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 AvaI C'yCGr_G Cuts at: 1870 1943 2813 3302 1870 Size: 73 870 489 4545 Fragments arranged by size: 4545 870 489 73 BamHI G'GATC_C Cuts at: 3307 3674 3307 Size: 367 5610 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2142 2787 3171 3298 4753 Size: 325 217 627 355 645 384 127 1455 Cuts at: 4753 618 Size: 1842 Fragments arranged by size: 1842 1455 645 627 384 355 325 217 127 BanII G_rGCy'C Cuts at: 1391 2607 3146 3296 1391 Size: 1216 539 150 4072 Fragments arranged by size: 4072 1216 539 150 BbeI G_GCGC'C Cuts at: 3175 3175 Size: 5977 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5006 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4168 4202 1903 Size: 34 50 34 2147 34 3678 Fragments arranged by size: 3678 2147 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3869 5079 3869 Size: 1210 4767 BclI T'GATC_A Cuts at: 1548 1548 Size: 5977 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2126 3116 3164 Size: 107 122 71 631 605 454 990 48 Cuts at: 3164 3180 4588 136 Size: 16 1408 1525 Fragments arranged by size: 1525 1408 990 631 605 454 122 107 71 48 16 BglII A'GATC_T Cuts at: 5972 5972 Size: 5977 BlpI GC'TnA_GC Cuts at: 2025 2156 2237 3135 3394 2025 Size: 131 81 898 259 4608 Fragments arranged by size: 4608 898 259 131 81 Bme1580I G_kGCm'C Cuts at: 1790 2307 2546 3146 4038 5194 1790 Size: 517 239 600 892 1156 2573 Fragments arranged by size: 2573 1156 892 600 517 239 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5977 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3708 4666 442 Size: 416 53 83 399 24 2291 958 1753 Fragments arranged by size: 2291 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5977 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2303 2581 2795 3002 3162 4637 1239 Size: 1064 278 214 207 160 1475 2579 Fragments arranged by size: 2579 1475 1064 278 214 207 160 Bpu10I CC'TnA_GC Cuts at: 2049 2049 Size: 5977 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2492 2591 2825 4102 4967 5265 2492 Size: 99 234 1277 865 298 3204 Fragments arranged by size: 3204 1277 865 298 234 99 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4640 882 Size: 617 3141 2219 Fragments arranged by size: 3141 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2341 2521 3220 3768 493 Size: 711 1137 180 699 548 2702 Fragments arranged by size: 2702 1137 711 699 548 180 BsaBI GATnn'nnATC Cuts at: 5971 5971 Size: 5977 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2629 3172 3784 4166 Size: 53 83 186 936 1096 543 612 382 Cuts at: 4166 4801 275 Size: 635 1451 Fragments arranged by size: 1451 1096 936 635 612 543 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4410 4808 5082 5229 5640 4410 Size: 398 274 147 411 4747 Fragments arranged by size: 4747 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2594 2624 2903 2933 1178 Size: 30 165 30 1191 30 279 30 4222 Fragments arranged by size: 4222 1191 279 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2300 2828 2831 1186 Size: 1114 528 3 4332 Fragments arranged by size: 4332 1114 528 3 BseYI C'CCAG_C Cuts at: 2882 3145 5180 5676 2882 Size: 263 2035 496 3183 Fragments arranged by size: 3183 2035 496 263 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2264 2282 2330 2543 3023 1163 Size: 573 528 18 48 213 480 4117 Fragments arranged by size: 4117 573 528 480 213 48 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2472 3427 4188 5216 1540 Size: 275 186 471 955 761 1028 2301 Fragments arranged by size: 2301 1028 955 761 471 275 186 BsiHKAI G_wGCw'C Cuts at: 2307 2546 2607 2699 2781 2921 3062 4038 4123 Size: 239 61 92 82 140 141 976 85 Cuts at: 4123 5194 2307 Size: 1071 3090 Fragments arranged by size: 3090 1071 976 239 141 140 92 85 82 61 BsmI GAATG_Cn' Cuts at: 3488 3581 3488 Size: 93 5884 BsmBI CGTCTCn'nnnn_ Cuts at: 2620 2620 Size: 5977 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2307 2546 2607 2699 2781 2921 3062 Size: 399 517 239 61 92 82 140 141 Cuts at: 3062 3146 3296 4038 4123 5194 1391 Size: 84 150 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 517 399 239 150 141 140 92 85 84 82 61 BspEI T'CCGG_A Cuts at: 5640 5640 Size: 5977 BspHI T'CATG_A Cuts at: 3072 3866 5596 3072 Size: 794 1730 3453 Fragments arranged by size: 3453 1730 794 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2254 2409 2790 3332 844 Size: 1410 155 381 542 3489 Fragments arranged by size: 3489 1410 542 381 155 BsrBI CCG'CTC Cuts at: 1394 1823 2019 2589 3864 1394 Size: 429 196 570 1275 3507 Fragments arranged by size: 3507 1275 570 429 196 BsrDI GCAATG_nn' Cuts at: 65 1248 3105 4472 4646 65 Size: 1183 1857 1367 174 1396 Fragments arranged by size: 1857 1396 1367 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2467 2471 4621 4808 1700 Size: 110 657 4 2150 187 2869 Fragments arranged by size: 2869 2150 657 187 110 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5977 BssHII G'CGCG_C Cuts at: 1925 2896 1925 Size: 971 5006 BssSI C'ACGA_G Cuts at: 3080 4037 5049 3080 Size: 957 1012 4008 Fragments arranged by size: 4008 1012 957 BstAPI GCAn_nnn'nTGC Cuts at: 794 2260 2311 3066 794 Size: 1466 51 755 3705 Fragments arranged by size: 3705 1466 755 51 BstBI TT'CG_AA Cuts at: 4781 4781 Size: 5977 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5977 BstXI CCAn_nnnn'nTGG Cuts at: 2077 2437 2996 2077 Size: 360 559 5058 Fragments arranged by size: 5058 559 360 BstYI r'GATC_y Cuts at: 1972 2374 2820 3307 3674 4059 4076 5528 5614 Size: 402 446 487 367 385 17 1452 86 Cuts at: 5614 5626 5972 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 487 446 402 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2070 2082 513 Size: 552 1005 12 4408 Fragments arranged by size: 4408 1005 552 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2782 487 Size: 683 56 1556 3682 Fragments arranged by size: 3682 1556 683 56 BtsI GCAGTG_nn' Cuts at: 2413 2442 3035 3153 3493 4287 4307 2413 Size: 29 593 118 340 794 20 4083 Fragments arranged by size: 4083 794 593 340 118 29 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5977 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5942 DraI TTT'AAA Cuts at: 3281 3281 Size: 5977 DraIII CAC_nnn'GTG Cuts at: 2311 3046 3066 2311 Size: 735 20 5222 Fragments arranged by size: 5222 735 20 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4984 809 Size: 507 3668 1802 Fragments arranged by size: 3668 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2067 2079 2162 Size: 54 1425 50 275 18 237 12 83 Cuts at: 2162 2469 2689 3424 4313 5942 8 Size: 307 220 735 889 1629 43 Fragments arranged by size: 1629 1425 889 735 307 275 237 220 83 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2469 3424 1537 Size: 275 657 955 4090 Fragments arranged by size: 4090 955 657 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2193 3907 1353 Size: 840 1714 3423 Fragments arranged by size: 3423 1714 840 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4564 4938 5084 387 Size: 1064 138 2975 374 146 1280 Fragments arranged by size: 2975 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2605 2605 Size: 5977 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2303 2581 2795 3002 3162 4040 4637 Size: 393 671 278 214 207 160 878 597 Cuts at: 4637 5424 5692 1239 Size: 787 268 1524 Fragments arranged by size: 1524 878 787 671 597 393 278 268 214 207 160 EcoO109I rG'GnC_Cy Cuts at: 2770 3142 2770 Size: 372 5605 EcoRI G'AATT_C Cuts at: 2639 3286 3664 2639 Size: 647 378 4952 Fragments arranged by size: 4952 647 378 FspI TGC'GCA Cuts at: 2405 4483 2405 Size: 2078 3899 HaeII r_GCGC'y Cuts at: 1678 2911 2977 3088 3122 3175 5124 1678 Size: 1233 66 111 34 53 1949 2531 Fragments arranged by size: 2531 1949 1233 111 66 53 34 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2108 2140 4618 4650 4692 Size: 32 135 32 536 32 2478 32 42 Cuts at: 4692 4724 1373 Size: 32 2626 Fragments arranged by size: 2626 2478 536 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3052 3321 3567 669 Size: 988 281 1114 269 246 3079 Fragments arranged by size: 3079 1114 988 281 269 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5977 HpaI GTT'AAC Cuts at: 3567 3567 Size: 5977 KasI G'GCGC_C Cuts at: 3171 3171 Size: 5977 KpnI G_GTAC'C Cuts at: 3302 3302 Size: 5977 MfeI C'AATT_G Cuts at: 3576 3576 Size: 5977 MluI A'CGCG_T Cuts at: 5853 5853 Size: 5977 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1992 2604 5091 5275 1305 Size: 687 612 2487 184 2007 Fragments arranged by size: 2487 2007 687 612 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2081 2164 2691 10 Size: 54 1425 343 249 83 527 3296 Fragments arranged by size: 3296 1425 527 343 249 83 54 MslI CAynn'nnrTG Cuts at: 518 1166 2075 2148 2708 2891 2994 3935 4294 Size: 648 909 73 560 183 103 941 359 Cuts at: 4294 4453 518 Size: 159 2042 Fragments arranged by size: 2042 941 909 648 560 359 183 159 103 73 MspA1I CmG'CkG Cuts at: 1606 2330 2425 2559 2959 3149 3393 4070 5218 Size: 724 95 134 400 190 244 677 1148 Cuts at: 5218 5463 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 724 677 400 245 244 190 134 95 NaeI GCC'GGC Cuts at: 2469 2469 Size: 5977 NarI GG'CG_CC Cuts at: 3172 3172 Size: 5977 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5425 NdeI CA'TA_TG Cuts at: 387 387 Size: 5977 NgoMIV G'CCGG_C Cuts at: 2467 2467 Size: 5977 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5977 NotI GC'GGCC_GC Cuts at: 3424 3424 Size: 5977 NspI r_CATG'y Cuts at: 1132 1229 2303 2917 3335 4880 1132 Size: 97 1074 614 418 1545 2229 Fragments arranged by size: 2229 1545 1074 614 418 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5977 PciI A'CATG_T Cuts at: 2299 2913 4876 2299 Size: 614 1963 3400 Fragments arranged by size: 3400 1963 614 PflMI CCAn_nnn'nTGG Cuts at: 1108 2857 3097 1108 Size: 1749 240 3988 Fragments arranged by size: 3988 1749 240 PfoI T'CCnGG_A Cuts at: 2667 2667 Size: 5977 PmeI GTTT'AAAC Cuts at: 3281 3281 Size: 5977 PmlI CAC'GTG Cuts at: 2341 2341 Size: 5977 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4046 4078 5559 5591 4046 Size: 32 1481 32 4432 Fragments arranged by size: 4432 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5977 PsiI TTA'TAA Cuts at: 3547 3547 Size: 5977 PspOMI G'GGCC_C Cuts at: 3142 3142 Size: 5977 PstI C_TGCA'G Cuts at: 830 2907 3126 3329 830 Size: 2077 219 203 3478 Fragments arranged by size: 3478 2077 219 203 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5977 PvuII CAG'CTG Cuts at: 2330 2425 3149 2330 Size: 95 724 5158 Fragments arranged by size: 5158 724 95 RsrII CG'GwC_CG Cuts at: 2554 2554 Size: 5977 SacI G_AGCT'C Cuts at: 2607 2607 Size: 5977 SalI G'TCGA_C Cuts at: 1936 3319 1936 Size: 1383 4594 SapI GCTCTTCn'nnn_ Cuts at: 2193 2193 Size: 5977 SbfI CC_TGCA'GG Cuts at: 3329 3329 Size: 5977 ScaI AGT'ACT Cuts at: 2846 4225 5937 2846 Size: 1379 1712 2886 Fragments arranged by size: 2886 1712 1379 SexAI A'CCwGG_T Cuts at: 2382 2946 2382 Size: 564 5413 SfcI C'TryA_G Cuts at: 826 1045 2903 3122 3325 4460 5141 5332 826 Size: 219 1858 219 203 1135 681 191 1471 Fragments arranged by size: 1858 1471 1135 681 219 219 203 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2126 2126 Size: 5977 SfoI GGC'GCC Cuts at: 3173 3173 Size: 5977 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5977 SmaI CCC'GGG Cuts at: 1872 3304 1872 Size: 1432 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2507 2606 2840 4081 4982 5244 Size: 197 926 564 99 234 1241 901 262 Cuts at: 5244 820 Size: 1553 Fragments arranged by size: 1553 1241 926 901 564 262 234 197 99 SnaBI TAC'GTA Cuts at: 493 3768 493 Size: 3275 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5977 SphI G_CATG'C Cuts at: 1132 3335 1132 Size: 2203 3774 SspI AAT'ATT Cuts at: 5 52 3901 5 Size: 47 3849 2081 Fragments arranged by size: 3849 2081 47 StuI AGG'CCT Cuts at: 1517 2120 2720 1517 Size: 603 600 4774 Fragments arranged by size: 4774 603 600 StyI C'CwwG_G Cuts at: 513 1065 2430 3416 513 Size: 552 1365 986 3074 Fragments arranged by size: 3074 1365 986 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2742 4004 4021 4174 4359 4804 325 Size: 1255 1162 1262 17 153 185 445 1498 Fragments arranged by size: 1498 1262 1255 1162 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2844 4223 5935 Size: 274 80 33 51 1443 867 1379 1712 Cuts at: 5935 96 Size: 138 Fragments arranged by size: 1712 1443 1379 867 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2286 2318 2794 2826 5937 5969 2286 Size: 32 476 32 3111 32 2294 Fragments arranged by size: 3111 2294 476 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5977 XbaI T'CTAG_A Cuts at: 3313 3313 Size: 5977 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3160 3160 Size: 5977 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5977 XmaI C'CCGG_G Cuts at: 1870 3302 1870 Size: 1432 4545 XmnI GAAnn'nnTTC Cuts at: 4106 4106 Size: 5977 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3785 276 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PpuMI PspXI PsrI SacII SanDI SrfI SwaI