Restriction map for
pFN21AB7033
(Linear) (Six-base) MAPSORT of: KIBB7033.seq Check: 8389 from: 1 to: 1272
KIBB7033.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 249 785 1272
Size: 249 536 487
Fragments arranged by size:
536 487 249
AfeI AGC'GCT
Cuts at: 0 904 970 1272
Size: 904 66 302
Fragments arranged by size:
904 302 66
AflIII A'CryG_T
Cuts at: 0 294 908 1272
Size: 294 614 364
Fragments arranged by size:
614 364 294
AleI CACnn'nnGTG
Cuts at: 0 70 143 886 989 1272
Size: 70 73 743 103 283
Fragments arranged by size:
743 283 103 73 70
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 904 1272
Size: 904 368
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 367 399 1272
Size: 367 32 873
Fragments arranged by size:
873 367 32
AlwNI CAG_nnn'CTG
Cuts at: 0 525 1092 1125 1180 1201 1272
Size: 525 567 33 55 21 71
Fragments arranged by size:
567 525 71 55 33 21
ApaI G_GGCC'C
Cuts at: 0 1141 1272
Size: 1141 131
ApaLI G'TGCA_C
Cuts at: 0 298 537 1272
Size: 298 239 735
Fragments arranged by size:
735 298 239
ApoI r'AATT_y
Cuts at: 0 634 1272
Size: 634 638
AvaI C'yCGr_G
Cuts at: 0 808 1272
Size: 808 464
BanI G'GyrC_C
Cuts at: 0 137 782 1166 1272
Size: 137 645 384 106
Fragments arranged by size:
645 384 137 106
BanII G_rGCy'C
Cuts at: 0 602 1141 1272
Size: 602 539 131
Fragments arranged by size:
602 539 131
BbeI G_GCGC'C
Cuts at: 0 1170 1272
Size: 1170 102
BglI GCCn_nnn'nGGC
Cuts at: 0 121 1111 1159 1175 1272
Size: 121 990 48 16 97
Fragments arranged by size:
990 121 97 48 16
BlpI GC'TnA_GC
Cuts at: 0 20 151 232 1130 1272
Size: 20 131 81 898 142
Fragments arranged by size:
898 142 131 81 20
Bme1580I G_kGCm'C
Cuts at: 0 302 541 1141 1272
Size: 302 239 600 131
Fragments arranged by size:
600 302 239 131
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 576 790 997 1157 1272
Size: 298 278 214 207 160 115
Fragments arranged by size:
298 278 214 207 160 115
Bpu10I CC'TnA_GC
Cuts at: 0 44 1272
Size: 44 1228
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 586 820 1272
Size: 487 99 234 452
Fragments arranged by size:
487 452 234 99
BsaAI yAC'GTr
Cuts at: 0 336 516 1215 1272
Size: 336 180 699 57
Fragments arranged by size:
699 336 180 57
BsaHI Gr'CG_yC
Cuts at: 0 624 1167 1272
Size: 624 543 105
Fragments arranged by size:
624 543 105
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 589 619 898 928 1272
Size: 589 30 279 30 344
Fragments arranged by size:
589 344 279 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 295 823 826 1272
Size: 295 528 3 446
Fragments arranged by size:
528 446 295 3
BseYI C'CCAG_C
Cuts at: 0 877 1140 1272
Size: 877 263 132
Fragments arranged by size:
877 263 132
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 277 325 538 1018 1272
Size: 259 18 48 213 480 254
Fragments arranged by size:
480 259 254 213 48 18
BsiEI CG_ry'CG
Cuts at: 0 467 1272
Size: 467 805
BsiHKAI G_wGCw'C
Cuts at: 0 302 541 602 694 776 916 1057 1272
Size: 302 239 61 92 82 140 141 215
Fragments arranged by size:
302 239 215 141 140 92 82 61
BsmBI CGTCTCn'nnnn_
Cuts at: 0 615 1272
Size: 615 657
Bsp1286I G_dGCh'C
Cuts at: 0 302 541 602 694 776 916 1057 1141
Size: 302 239 61 92 82 140 141 84
Cuts at: 1141 1272
Size: 131
Fragments arranged by size:
302 239 141 140 131 92 84 82
61
BspHI T'CATG_A
Cuts at: 0 1067 1272
Size: 1067 205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 249 404 785 1272
Size: 249 155 381 487
Fragments arranged by size:
487 381 249 155
BsrBI CCG'CTC
Cuts at: 0 14 584 1272
Size: 14 570 688
Fragments arranged by size:
688 570 14
BsrDI GCAATG_nn'
Cuts at: 0 1100 1272
Size: 1100 172
BsrFI r'CCGG_y
Cuts at: 0 462 466 1272
Size: 462 4 806
Fragments arranged by size:
806 462 4
BssHII G'CGCG_C
Cuts at: 0 891 1272
Size: 891 381
BssSI C'ACGA_G
Cuts at: 0 1075 1272
Size: 1075 197
BstAPI GCAn_nnn'nTGC
Cuts at: 0 255 306 1061 1272
Size: 255 51 755 211
Fragments arranged by size:
755 255 211 51
BstXI CCAn_nnnn'nTGG
Cuts at: 0 72 432 991 1272
Size: 72 360 559 281
Fragments arranged by size:
559 360 281 72
BstYI r'GATC_y
Cuts at: 0 369 815 1272
Size: 369 446 457
Fragments arranged by size:
457 446 369
BtgI C'CryG_G
Cuts at: 0 65 77 1272
Size: 65 12 1195
Fragments arranged by size:
1195 65 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 777 1272
Size: 777 495
BtsI GCAGTG_nn'
Cuts at: 0 408 437 1030 1148 1272
Size: 408 29 593 118 124
Fragments arranged by size:
593 408 124 118 29
DraIII CAC_nnn'GTG
Cuts at: 0 306 1041 1061 1272
Size: 306 735 20 211
Fragments arranged by size:
735 306 211 20
EaeI y'GGCC_r
Cuts at: 0 62 74 157 464 684 1272
Size: 62 12 83 307 220 588
Fragments arranged by size:
588 307 220 83 62 12
EagI C'GGCC_G
Cuts at: 0 464 1272
Size: 464 808
EarI CTCTTCn'nnn_
Cuts at: 0 188 1272
Size: 188 1084
EcoICRI GAG'CTC
Cuts at: 0 600 1272
Size: 600 672
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 576 790 997 1157 1272
Size: 298 278 214 207 160 115
Fragments arranged by size:
298 278 214 207 160 115
EcoO109I rG'GnC_Cy
Cuts at: 0 765 1137 1272
Size: 765 372 135
Fragments arranged by size:
765 372 135
EcoRI G'AATT_C
Cuts at: 0 634 1272
Size: 634 638
FspI TGC'GCA
Cuts at: 0 400 1272
Size: 400 872
HaeII r_GCGC'y
Cuts at: 0 906 972 1083 1117 1170 1272
Size: 906 66 111 34 53 102
Fragments arranged by size:
906 111 102 66 53 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 103 135 1272
Size: 103 32 1137
Fragments arranged by size:
1137 103 32
HincII GTy'rAC
Cuts at: 0 1047 1272
Size: 1047 225
KasI G'GCGC_C
Cuts at: 0 1166 1272
Size: 1166 106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 599 1272
Size: 599 673
MscI TGG'CCA
Cuts at: 0 76 159 686 1272
Size: 76 83 527 586
Fragments arranged by size:
586 527 83 76
MslI CAynn'nnrTG
Cuts at: 0 70 143 703 886 989 1272
Size: 70 73 560 183 103 283
Fragments arranged by size:
560 283 183 103 73 70
MspA1I CmG'CkG
Cuts at: 0 325 420 554 954 1144 1272
Size: 325 95 134 400 190 128
Fragments arranged by size:
400 325 190 134 128 95
NaeI GCC'GGC
Cuts at: 0 464 1272
Size: 464 808
NarI GG'CG_CC
Cuts at: 0 1167 1272
Size: 1167 105
NgoMIV G'CCGG_C
Cuts at: 0 462 1272
Size: 462 810
NspI r_CATG'y
Cuts at: 0 298 912 1272
Size: 298 614 360
Fragments arranged by size:
614 360 298
PciI A'CATG_T
Cuts at: 0 294 908 1272
Size: 294 614 364
Fragments arranged by size:
614 364 294
PflMI CCAn_nnn'nTGG
Cuts at: 0 852 1092 1272
Size: 852 240 180
Fragments arranged by size:
852 240 180
PfoI T'CCnGG_A
Cuts at: 0 662 1272
Size: 662 610
PmlI CAC'GTG
Cuts at: 0 336 1272
Size: 336 936
PspOMI G'GGCC_C
Cuts at: 0 1137 1272
Size: 1137 135
PstI C_TGCA'G
Cuts at: 0 902 1121 1272
Size: 902 219 151
Fragments arranged by size:
902 219 151
PvuII CAG'CTG
Cuts at: 0 325 420 1144 1272
Size: 325 95 724 128
Fragments arranged by size:
724 325 128 95
RsrII CG'GwC_CG
Cuts at: 0 549 1272
Size: 549 723
SacI G_AGCT'C
Cuts at: 0 602 1272
Size: 602 670
SapI GCTCTTCn'nnn_
Cuts at: 0 188 1272
Size: 188 1084
ScaI AGT'ACT
Cuts at: 0 841 1272
Size: 841 431
SexAI A'CCwGG_T
Cuts at: 0 377 941 1272
Size: 377 564 331
Fragments arranged by size:
564 377 331
SfcI C'TryA_G
Cuts at: 0 898 1117 1272
Size: 898 219 155
Fragments arranged by size:
898 219 155
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 121 1272
Size: 121 1151
SfoI GGC'GCC
Cuts at: 0 1168 1272
Size: 1168 104
SmlI C'TyrA_G
Cuts at: 0 502 601 835 1272
Size: 502 99 234 437
Fragments arranged by size:
502 437 234 99
StuI AGG'CCT
Cuts at: 0 115 715 1272
Size: 115 600 557
Fragments arranged by size:
600 557 115
StyI C'CwwG_G
Cuts at: 0 425 1272
Size: 425 847
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 737 1272
Size: 737 535
TatI w'GTAC_w
Cuts at: 0 839 1272
Size: 839 433
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 281 313 789 821 1272
Size: 281 32 476 32 451
Fragments arranged by size:
476 451 281 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1155 1272
Size: 1155 117
Enzymes that do cut:
AarI AfeI AflIII AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbeI BglI BlpI
Bme1580I BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstXI BstYI
BtgI BtgZI BtsI DraIII EaeI EagI EarI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PfoI PmlI PspOMI PstI PvuII RsrII
SacI SapI ScaI SexAI SfcI SfiI SfoI SmlI
StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AgeI AhdI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BmgBI BmrI BmtI
BplI BsaI BsaBI BsaWI BsiWI BsmI BspEI BsrGI
BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DrdI
EciI EcoNI EcoRV FalI FseI FspAI HindIII HpaI
KpnI MfeI MluI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PmeI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI SacII SalI SanDI SbfI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI