Restriction map for
pFN21AB7032
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
AspEI AspHI AspI AvrII BamHI
BanII BbsI BcgI BciVI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrFI BsrGI
BssSI Bst1107I BstBI BstZ17I CelII
Cfr10I ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRI EcoRV EcoT22I FseI HincII
HindII HindIII HpaI KspI MaeI
MamI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 70 enzymes) #
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Acc65I 1218 GGTACC G^GTACC
AcyI 206 GGCGCC G[GA]^CG[CT]C
AfaI 1219 GTAC GT^AC
AlwI 951 GATCC GGATC(4/5)
AlwNI 842 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 642 CCTGAGG CC^T[ACGT]AGG
Asp700I 1163 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1218 GGTACC G^GTACC
AvaI 1064 CCCGAG C^[CT]CG[GA]G
AvaII 195 GGACC G^G[AT]CC
BbeI 206 GGCGCC GGCGC^C
BbrPI 805 CACGTG CAC^GTG
BbvCI 943 GCTGAGG CCTCAGC(-5/-2)
BcnI 24 CCCGG CC^[GC]GG
BmyI 1061 GTGCCC G[AGT]GC[ACT]^C
BsaAI 805 CACGTG [CT]AC^GT[GA]
BsaHI 206 GGCGCC G[GA]^CG[CT]C
BsaI 83 GAGACC GGTCTC(1/5)
BsgI 1056 GTGCAG GTGCAG(16/14)
BsmI 451 GAATGC GAATGC(1/-1)
BsoBI 1064 CCCGAG C^[CT]CG[GA]G
Bsp1286I 1061 GTGCCC G[AGT]GC[ACT]^C
BspHI 533 TCATGA T^CATGA
BspMI 62 ACCTGC ACCTGC(4/8)
BsrBI 117 GAGCGG CCGCTC(-3/-3)
BsrDI 939 CATTGC GCAATG(2/0)
BssHII 43 GCGCGC G^CGCGC
BstAPI 611 GCAAGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 637 GGTGACC G^GT[ACGT]ACC
BstPI 637 GGTGACC G^GT[ACGT]ACC
BstXI 185 CCACCTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 950 AGATCC [GA]^GATC[CT]
Bsu36I 642 CCTGAGG CC^T[ACGT]AGG
DraII 121 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 351 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 865 GACTTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 642 CCTGAGG CC^T[ACGT]AGG
EcoO109I 121 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 637 GGTGACC G^GT[ACGT]ACC
HaeII 206 GGCGCC [GA]GCGC^[CT]
HapII 25 CCGG C^CGG
HgaI 161 GACGC GACGC(5/10)
Hin1I 206 GGCGCC G[GA]^CG[CT]C
HpaII 25 CCGG C^CGG
KasI 206 GGCGCC G^GCGCC
KpnI 1218 GGTACC GGTAC^C
MaeII 806 ACGT A^CGT
MflI 950 AGATCC [GA]^GATC[CT]
MseI 1037 TTAA T^TAA
MspA1I 246 CAGCTG C[AC]G^C[GT]G
MspI 25 CCGG C^CGG
NarI 206 GGCGCC GG^CGCC
NciI 24 CCCGG CC^[GC]GG
NcoI 291 CCATGG C^CATGG
PmaCI 805 CACGTG CAC^GTG
PmlI 805 CACGTG CAC^GTG
PshAI 363 GACTAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 243 CTGCAG CTGCA^G
PvuII 246 CAGCTG CAG^CTG
RcaI 533 TCATGA T^CATGA
RsaI 1219 GTAC GT^AC
SapI 255 GCTCTTC GCTCTTC(1/4)
SfoI 206 GGCGCC GGC^GCC
SspI 487 AATATT AAT^ATT
TaqI 761 TCGA T^CGA
Tru9I 1037 TTAA T^TAA
TthHB8I 761 TCGA T^CGA
XcmI 857 CCAACCAAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 950 AGATCC [GA]^GATC[CT]
XmnI 1163 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 44 CGCG CG^CG
74 CGCG
AcsI 1021 AAATTT [GA]^AATT[CT]
1131 AAATTC
ApoI 1021 AAATTT [GA]^AATT[CT]
1131 AAATTC
AviII 65 TGCGCA TGC^GCA
674 TGCGCA
BanI 206 GGCGCC G^G[CT][GA]CC
1218 GGTACC
BpmI 481 CTCCAG CTGGAG(16/14)
1241 CTCCAG
BsmAI 83 GAGAC GTCTC(1/5)
1122 GTCTC
BsmFI 296 GGGAC GGGAC(10/14)
824 GTCCC
BsrI 735 ACTGG ACTGG(1/-1)
907 ACTGG
BstUI 44 CGCG CG^CG
74 CGCG
DsaI 291 CCATGG C^C[GA][CT]GG
1051 CCACGG
EarI 256 CTCTTC CTCTTC(1/4)
394 CTCTTC
EcoT14I 188 CCTTGG C^C[AT][AT]GG
291 CCATGG
FauI 101 CCCGC CCCGC(4/6)
119 GCGGG
FspI 65 TGCGCA TGC^GCA
674 TGCGCA
Ksp632I 256 CTCTTC CTCTTC(1/4)
394 CTCTTC
MvnI 44 CGCG CG^CG
74 CGCG
PflMI 210 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
861 CCAAGACTTGG
PleI 1239 GACTC GAGTC(4/5)
346 GAGTC
SfcI 169 CTACAG C^T[GA][CT]AG
243 CTGCAG
StyI 188 CCTTGG C^C[AT][AT]GG
291 CCATGG
TfiI 477 GATTC G^A[AT]TC
1267 GAATC
Van91I 210 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
861 CCAAGACTTGG
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI BalI BbvI BclI
BsaJI BseRI BsiYI BslI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EcoRII FbaI
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI MaeIII MboI
MboII MluNI MnlI MscI MslI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI TseI
Tsp45I Tsp509I TspRI