(Circular) (Six-base) MAPSORT of: pFN21AB7032.seq Check: 8572 from: 1 to: 5977 pFN21AB7032 5977 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3787 278 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3320 1937 Size: 1383 4594 Acc65I G'GTAC_C Cuts at: 3223 3298 3223 Size: 75 5902 AclI AA'CG_TT Cuts at: 1563 4104 4477 1563 Size: 2541 373 3063 Fragments arranged by size: 3063 2541 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2733 4040 5424 5692 1632 Size: 1101 1307 1384 268 1917 Fragments arranged by size: 1917 1384 1307 1101 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5780 AflIII A'CryG_T Cuts at: 1203 4876 5853 1203 Size: 3673 977 1327 Fragments arranged by size: 3673 1327 977 AgeI A'CCGG_T Cuts at: 4808 4808 Size: 5977 AhdI GACnn_n'nnGTC Cuts at: 4706 4706 Size: 5977 AleI CACnn'nnGTG Cuts at: 1166 2195 1166 Size: 1029 4948 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2273 1184 Size: 1089 4888 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3197 3229 3197 Size: 32 5945 AlwNI CAG_nnn'CTG Cuts at: 2852 3720 5292 2852 Size: 868 1572 3537 Fragments arranged by size: 3537 1572 868 ApaLI G'TGCA_C Cuts at: 4034 5190 4034 Size: 1156 4821 ApoI r'AATT_y Cuts at: 1493 3026 3136 3286 3512 3664 5773 1493 Size: 1533 110 150 226 152 2109 1697 Fragments arranged by size: 2109 1697 1533 226 152 150 110 AseI AT'TA_AT Cuts at: 160 1032 4531 160 Size: 872 3499 1606 Fragments arranged by size: 3499 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 AvaI C'yCGr_G Cuts at: 1870 1943 3069 3302 1870 Size: 73 1126 233 4545 Fragments arranged by size: 4545 1126 233 73 BamHI G'GATC_C Cuts at: 3307 3674 3307 Size: 367 5610 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2211 3223 3298 4753 618 Size: 325 217 627 424 1012 75 1455 1842 Fragments arranged by size: 1842 1455 1012 627 424 325 217 75 BanII G_rGCy'C Cuts at: 1391 3296 1391 Size: 1905 4072 BbeI G_GCGC'C Cuts at: 2215 2215 Size: 5977 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5006 BbvCI CC'TCA_GC Cuts at: 2949 2949 Size: 5977 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4168 4202 1903 Size: 34 2231 34 3678 Fragments arranged by size: 3678 2231 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3869 5079 3869 Size: 1210 4767 BclI T'GATC_A Cuts at: 1548 2505 2661 2799 1548 Size: 957 156 138 4726 Fragments arranged by size: 4726 957 156 138 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4588 136 Size: 107 122 71 631 605 337 2579 1525 Fragments arranged by size: 2579 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5972 5972 Size: 5977 BlpI GC'TnA_GC Cuts at: 3394 3394 Size: 5977 Bme1580I G_kGCm'C Cuts at: 1790 3070 4038 5194 1790 Size: 1280 968 1156 2573 Fragments arranged by size: 2573 1280 1156 968 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5977 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3708 4666 442 Size: 416 53 83 399 24 2291 958 1753 Fragments arranged by size: 2291 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5977 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2111 2111 Size: 5977 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2471 3231 4637 1239 Size: 1232 760 1406 2579 Fragments arranged by size: 2579 1406 1232 760 Bpu10I CC'TnA_GC Cuts at: 2550 2949 2550 Size: 399 5578 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4102 4967 5265 4102 Size: 865 298 4814 Fragments arranged by size: 4814 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2082 4640 882 Size: 617 583 2558 2219 Fragments arranged by size: 2558 2219 617 583 BsaAI yAC'GTr Cuts at: 493 1204 2812 3768 493 Size: 711 1608 956 2702 Fragments arranged by size: 2702 1608 956 711 BsaBI GATnn'nnATC Cuts at: 5971 5971 Size: 5977 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2212 3784 4166 4801 Size: 53 83 186 936 679 1572 382 635 Cuts at: 4801 275 Size: 1451 Fragments arranged by size: 1572 1451 936 679 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4410 4808 5082 5229 5640 4410 Size: 398 274 147 411 4747 Fragments arranged by size: 4747 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5752 Fragments arranged by size: 5752 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 2966 3196 1186 Size: 862 918 230 3967 Fragments arranged by size: 3967 918 862 230 BseYI C'CCAG_C Cuts at: 5180 5676 5180 Size: 496 5481 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3082 1163 Size: 573 1346 4058 Fragments arranged by size: 4058 1346 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3427 4188 5216 1540 Size: 275 186 1426 761 1028 2301 Fragments arranged by size: 2301 1426 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4038 4123 5194 4038 Size: 85 1071 4821 Fragments arranged by size: 4821 1071 85 BsmI GAATG_Cn' Cuts at: 2462 3488 3581 2462 Size: 1026 93 4858 Fragments arranged by size: 4858 1026 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3070 3296 4038 4123 5194 1391 Size: 399 1280 226 742 85 1071 2174 Fragments arranged by size: 2174 1280 1071 742 399 226 85 BspEI T'CCGG_A Cuts at: 5640 5640 Size: 5977 BspHI T'CATG_A Cuts at: 2538 3866 5596 2538 Size: 1328 1730 2919 Fragments arranged by size: 2919 1730 1328 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2076 3332 844 Size: 1232 1256 3489 Fragments arranged by size: 3489 1256 1232 BsrBI CCG'CTC Cuts at: 1394 1823 2124 3864 1394 Size: 429 301 1740 3507 Fragments arranged by size: 3507 1740 429 301 BsrDI GCAATG_nn' Cuts at: 65 1248 2943 4472 4646 65 Size: 1183 1695 1529 174 1396 Fragments arranged by size: 1695 1529 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4621 4808 1700 Size: 110 2811 187 2869 Fragments arranged by size: 2869 2811 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5977 BssHII G'CGCG_C Cuts at: 1925 2048 1925 Size: 123 5854 BssSI C'ACGA_G Cuts at: 4037 5049 4037 Size: 1012 4965 BstAPI GCAn_nnn'nTGC Cuts at: 794 2622 794 Size: 1828 4149 BstBI TT'CG_AA Cuts at: 4781 4781 Size: 5977 BstEII G'GTnAC_C Cuts at: 1184 2642 1184 Size: 1458 4519 BstXI CCAn_nnnn'nTGG Cuts at: 2197 2197 Size: 5977 BstYI r'GATC_y Cuts at: 1972 2955 3307 3674 4059 4076 5528 5614 5626 Size: 983 352 367 385 17 1452 86 12 Cuts at: 5626 5972 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 983 385 367 352 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2648 2648 Size: 5977 BtgI C'CryG_G Cuts at: 513 1065 2296 3056 513 Size: 552 1231 760 3434 Fragments arranged by size: 3434 1231 760 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2038 2098 487 Size: 683 56 812 60 4366 Fragments arranged by size: 4366 812 683 60 56 BtsI GCAGTG_nn' Cuts at: 3070 3493 4287 4307 3070 Size: 423 794 20 4740 Fragments arranged by size: 4740 794 423 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5977 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5942 DraI TTT'AAA Cuts at: 3281 3281 Size: 5977 DraIII CAC_nnn'GTG Cuts at: 2361 2361 Size: 5977 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2876 4984 809 Size: 507 1560 2108 1802 Fragments arranged by size: 2108 1802 1560 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2466 2859 Size: 54 1425 50 275 18 180 456 393 Cuts at: 2859 3424 4313 5942 8 Size: 565 889 1629 43 Fragments arranged by size: 1629 1425 889 565 456 393 275 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3424 1537 Size: 275 1612 4090 Fragments arranged by size: 4090 1612 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2267 2405 3907 1353 Size: 914 138 1502 3423 Fragments arranged by size: 3423 1502 914 138 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4564 4938 5084 387 Size: 1064 138 2975 374 146 1280 Fragments arranged by size: 2975 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2471 2733 3231 4040 4637 5424 5692 Size: 393 839 262 498 809 597 787 268 Cuts at: 5692 1239 Size: 1524 Fragments arranged by size: 1524 839 809 787 597 498 393 268 262 EcoO109I rG'GnC_Cy Cuts at: 2127 2127 Size: 5977 EcoRI G'AATT_C Cuts at: 3286 3664 3286 Size: 378 5599 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2641 2641 Size: 5977 FspI TGC'GCA Cuts at: 2072 2681 4483 2072 Size: 609 1802 3566 Fragments arranged by size: 3566 1802 609 FspAI rTGC'GCAy Cuts at: 2681 2681 Size: 5977 HaeII r_GCGC'y Cuts at: 1678 2215 5124 1678 Size: 537 2909 2531 Fragments arranged by size: 2909 2531 537 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4618 4650 4692 4724 1373 Size: 32 135 32 3046 32 42 32 2626 Fragments arranged by size: 3046 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3321 3567 669 Size: 988 281 1383 246 3079 Fragments arranged by size: 3079 1383 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5977 HpaI GTT'AAC Cuts at: 3567 3567 Size: 5977 KasI G'GCGC_C Cuts at: 2211 2211 Size: 5977 KpnI G_GTAC'C Cuts at: 3227 3302 3227 Size: 75 5902 MfeI C'AATT_G Cuts at: 3576 3576 Size: 5977 MluI A'CGCG_T Cuts at: 5853 5853 Size: 5977 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5091 5275 1305 Size: 3786 184 2007 Fragments arranged by size: 3786 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2012 2468 2861 10 Size: 54 1425 343 180 456 393 3126 Fragments arranged by size: 3126 1425 456 393 343 180 54 MslI CAynn'nnrTG Cuts at: 518 1166 2195 2537 2687 3935 4294 4453 518 Size: 648 1029 342 150 1248 359 159 2042 Fragments arranged by size: 2042 1248 1029 648 359 342 159 150 MspA1I CmG'CkG Cuts at: 1606 2253 3393 4070 5218 5463 1606 Size: 647 1140 677 1148 245 2120 Fragments arranged by size: 2120 1148 1140 677 647 245 NarI GG'CG_CC Cuts at: 2212 2212 Size: 5977 NcoI C'CATG_G Cuts at: 513 1065 2296 513 Size: 552 1231 4194 Fragments arranged by size: 4194 1231 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5977 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5977 NotI GC'GGCC_GC Cuts at: 3424 3424 Size: 5977 NspI r_CATG'y Cuts at: 1132 1229 3335 4880 1132 Size: 97 2106 1545 2229 Fragments arranged by size: 2229 2106 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5977 PciI A'CATG_T Cuts at: 4876 4876 Size: 5977 PflMI CCAn_nnn'nTGG Cuts at: 1108 2221 2872 1108 Size: 1113 651 4213 Fragments arranged by size: 4213 1113 651 PfoI T'CCnGG_A Cuts at: 2028 2028 Size: 5977 PmeI GTTT'AAAC Cuts at: 3281 3281 Size: 5977 PmlI CAC'GTG Cuts at: 2812 2812 Size: 5977 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4046 4078 5559 5591 4046 Size: 32 1481 32 4432 Fragments arranged by size: 4432 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2372 1613 Size: 759 5218 PsiI TTA'TAA Cuts at: 3547 3547 Size: 5977 PstI C_TGCA'G Cuts at: 830 2252 3329 830 Size: 1422 1077 3478 Fragments arranged by size: 3478 1422 1077 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5977 PvuII CAG'CTG Cuts at: 2253 2253 Size: 5977 SalI G'TCGA_C Cuts at: 1936 3319 1936 Size: 1383 4594 SapI GCTCTTCn'nnn_ Cuts at: 2267 2267 Size: 5977 SbfI CC_TGCA'GG Cuts at: 3329 3329 Size: 5977 ScaI AGT'ACT Cuts at: 4225 5937 4225 Size: 1712 4265 SfcI C'TryA_G Cuts at: 826 1045 2174 2248 3325 4460 5141 5332 826 Size: 219 1129 74 1077 1135 681 191 1471 Fragments arranged by size: 1471 1135 1129 1077 681 219 191 74 SfoI GGC'GCC Cuts at: 2213 2213 Size: 5977 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5977 SmaI CCC'GGG Cuts at: 1872 3304 1872 Size: 1432 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4081 4982 5244 820 Size: 197 926 2138 901 262 1553 Fragments arranged by size: 2138 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3768 493 Size: 3275 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5977 SphI G_CATG'C Cuts at: 1132 3335 1132 Size: 2203 3774 SspI AAT'ATT Cuts at: 5 52 2494 3901 5 Size: 47 2442 1407 2081 Fragments arranged by size: 2442 2081 1407 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5977 StyI C'CwwG_G Cuts at: 513 1065 2193 2296 3416 513 Size: 552 1128 103 1120 3074 Fragments arranged by size: 3074 1128 1120 552 103 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2630 4004 4021 4174 4359 4804 325 Size: 1255 1050 1374 17 153 185 445 1498 Fragments arranged by size: 1498 1374 1255 1050 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4223 5935 96 Size: 274 80 33 51 1443 2246 1712 138 Fragments arranged by size: 2246 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5937 5969 5937 Size: 32 5945 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5977 XbaI T'CTAG_A Cuts at: 3313 3313 Size: 5977 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2869 2869 Size: 5977 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5977 XmaI C'CCGG_G Cuts at: 1870 3302 1870 Size: 1432 4545 XmnI GAAnn'nnTTC Cuts at: 3172 4106 3172 Size: 934 5043 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3785 276 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI NaeI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI