(Circular) (Six-base) MAPSORT of: pFN21AB7031.seq Check: 8864 from: 1 to: 5824 pFN21AB7031 5824 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3634 278 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2142 3167 1937 Size: 205 1025 4594 Fragments arranged by size: 4594 1025 205 Acc65I G'GTAC_C Cuts at: 3145 3145 Size: 5824 AclI AA'CG_TT Cuts at: 1563 3951 4324 1563 Size: 2388 373 3063 Fragments arranged by size: 3063 2388 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2566 2869 3887 5271 5539 1632 Size: 934 303 1018 1384 268 1917 Fragments arranged by size: 1917 1384 1018 934 303 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5627 AflIII A'CryG_T Cuts at: 1203 2370 4723 5700 1203 Size: 1167 2353 977 1327 Fragments arranged by size: 2353 1327 1167 977 AgeI A'CCGG_T Cuts at: 4655 4655 Size: 5824 AhdI GACnn_n'nnGTC Cuts at: 4553 4553 Size: 5824 AleI CACnn'nnGTG Cuts at: 1166 2732 1166 Size: 1566 4258 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2441 1184 Size: 1257 4567 AlwNI CAG_nnn'CTG Cuts at: 3567 5139 3567 Size: 1572 4252 ApaLI G'TGCA_C Cuts at: 3881 5037 3881 Size: 1156 4668 ApoI r'AATT_y Cuts at: 1493 3133 3359 3511 5620 1493 Size: 1640 226 152 2109 1697 Fragments arranged by size: 2109 1697 1640 226 152 AseI AT'TA_AT Cuts at: 160 1032 4378 160 Size: 872 3346 1606 Fragments arranged by size: 3346 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 AvaI C'yCGr_G Cuts at: 1870 1943 2162 3149 1870 Size: 73 219 987 4545 Fragments arranged by size: 4545 987 219 73 BamHI G'GATC_C Cuts at: 3154 3521 3154 Size: 367 5457 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2823 3145 4600 618 Size: 325 217 627 1036 322 1455 1842 Fragments arranged by size: 1842 1455 1036 627 325 322 217 BanII G_rGCy'C Cuts at: 1391 2856 3143 1391 Size: 1465 287 4072 Fragments arranged by size: 4072 1465 287 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2222 2266 2377 2524 2595 2875 928 Size: 971 323 44 111 147 71 280 3877 Fragments arranged by size: 3877 971 323 280 147 111 71 44 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4015 4049 1903 Size: 34 2078 34 3678 Fragments arranged by size: 3678 2078 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3716 4926 3716 Size: 1210 4614 BclI T'GATC_A Cuts at: 1548 2187 1548 Size: 639 5185 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4435 136 Size: 107 122 71 631 605 2763 1525 Fragments arranged by size: 2763 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5819 5819 Size: 5824 BlpI GC'TnA_GC Cuts at: 3241 3241 Size: 5824 Bme1580I G_kGCm'C Cuts at: 1790 2828 3885 5041 1790 Size: 1038 1057 1156 2573 Fragments arranged by size: 2573 1156 1057 1038 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5824 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2326 2693 3555 Size: 416 53 83 399 24 909 367 862 Cuts at: 3555 4513 442 Size: 958 1753 Fragments arranged by size: 1753 958 909 862 416 399 367 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5824 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4484 1239 Size: 3245 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2533 3949 4814 5112 2533 Size: 1416 865 298 3245 Fragments arranged by size: 3245 1416 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3104 4487 882 Size: 617 1605 1383 2219 Fragments arranged by size: 2219 1605 1383 617 BsaAI yAC'GTr Cuts at: 493 1204 3615 493 Size: 711 2411 2702 Fragments arranged by size: 2702 2411 711 BsaBI GATnn'nnATC Cuts at: 5818 5818 Size: 5824 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3631 4013 4648 275 Size: 53 83 186 936 2098 382 635 1451 Fragments arranged by size: 2098 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4257 4655 4929 5076 5487 4257 Size: 398 274 147 411 4594 Fragments arranged by size: 4594 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2829 2859 1178 Size: 30 165 30 1426 30 4143 Fragments arranged by size: 4143 1426 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2420 1186 Size: 1234 4590 BseYI C'CCAG_C Cuts at: 3047 5027 5523 3047 Size: 1980 496 3348 Fragments arranged by size: 3348 1980 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5251 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3274 4035 5063 1540 Size: 275 186 1273 761 1028 2301 Fragments arranged by size: 2301 1273 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2430 2856 3885 3970 5041 2430 Size: 426 1029 85 1071 3213 Fragments arranged by size: 3213 1071 1029 426 85 BsmI GAATG_Cn' Cuts at: 3335 3428 3335 Size: 93 5731 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2430 2828 2856 3143 3885 3970 5041 Size: 399 640 398 28 287 742 85 1071 Cuts at: 5041 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 640 399 398 287 85 28 BspEI T'CCGG_A Cuts at: 5487 5487 Size: 5824 BspHI T'CATG_A Cuts at: 3713 5443 3713 Size: 1730 4094 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2559 3179 844 Size: 1715 620 3489 Fragments arranged by size: 3489 1715 620 BsrBI CCG'CTC Cuts at: 1394 1823 3711 1394 Size: 429 1888 3507 Fragments arranged by size: 3507 1888 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2182 2193 2919 4319 4493 65 Size: 1183 934 11 726 1400 174 1396 Fragments arranged by size: 1400 1396 1183 934 726 174 11 BsrFI r'CCGG_y Cuts at: 1700 1810 2649 3113 4468 4655 1700 Size: 110 839 464 1355 187 2869 Fragments arranged by size: 2869 1355 839 464 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5824 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5824 BssSI C'ACGA_G Cuts at: 2608 3884 4896 2608 Size: 1276 1012 3536 Fragments arranged by size: 3536 1276 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2154 794 Size: 1360 4464 BstBI TT'CG_AA Cuts at: 4628 4628 Size: 5824 BstEII G'GTnAC_C Cuts at: 1184 2116 1184 Size: 932 4892 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5824 BstYI r'GATC_y Cuts at: 1972 2124 3154 3521 3906 3923 5375 5461 5473 Size: 152 1030 367 385 17 1452 86 12 Cuts at: 5473 5819 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1030 385 367 346 152 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2304 513 Size: 552 1239 4033 Fragments arranged by size: 4033 1239 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5085 Fragments arranged by size: 5085 683 56 BtsI GCAGTG_nn' Cuts at: 2256 2862 2907 3340 4134 4154 2256 Size: 606 45 433 794 20 3926 Fragments arranged by size: 3926 794 606 433 45 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5824 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2896 2931 529 Size: 35 2332 35 3422 Fragments arranged by size: 3422 2332 35 35 DraI TTT'AAA Cuts at: 3128 3128 Size: 5824 DraIII CAC_nnn'GTG Cuts at: 2659 2659 Size: 5824 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4831 809 Size: 507 3515 1802 Fragments arranged by size: 3515 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2760 2904 3271 Size: 54 1425 50 275 18 930 144 367 Cuts at: 3271 4160 5789 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 930 889 367 275 144 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3271 1537 Size: 275 1459 4090 Fragments arranged by size: 4090 1459 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2999 3043 3754 1353 Size: 1646 44 711 3423 Fragments arranged by size: 3423 1646 711 44 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4411 4785 4931 387 Size: 1064 138 2822 374 146 1280 Fragments arranged by size: 2822 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2854 2854 Size: 5824 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2566 2869 3887 4484 5271 5539 1239 Size: 393 934 303 1018 597 787 268 1524 Fragments arranged by size: 1524 1018 934 787 597 393 303 268 EcoO109I rG'GnC_Cy Cuts at: 2497 2497 Size: 5824 EcoRI G'AATT_C Cuts at: 3133 3511 3133 Size: 378 5446 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2794 2794 Size: 5824 FspI TGC'GCA Cuts at: 4330 4330 Size: 5824 HaeII r_GCGC'y Cuts at: 1678 4971 1678 Size: 3293 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2081 2113 4465 4497 4539 Size: 32 135 32 509 32 2352 32 42 Cuts at: 4539 4571 1373 Size: 32 2626 Fragments arranged by size: 2626 2352 509 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3168 3414 669 Size: 988 281 1230 246 3079 Fragments arranged by size: 3079 1230 988 281 246 HindIII A'AGCT_T Cuts at: 748 3058 748 Size: 2310 3514 HpaI GTT'AAC Cuts at: 3414 3414 Size: 5824 KpnI G_GTAC'C Cuts at: 3149 3149 Size: 5824 MfeI C'AATT_G Cuts at: 3423 3423 Size: 5824 MluI A'CGCG_T Cuts at: 5700 5700 Size: 5824 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4938 5122 1305 Size: 3633 184 2007 Fragments arranged by size: 3633 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2762 2906 10 Size: 54 1425 343 930 144 2928 Fragments arranged by size: 2928 1425 930 343 144 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2423 2660 2732 2867 3782 4141 Size: 648 843 414 237 72 135 915 359 Cuts at: 4141 4300 518 Size: 159 2042 Fragments arranged by size: 2042 915 843 648 414 359 237 159 135 72 MspA1I CmG'CkG Cuts at: 1606 2415 2931 3240 3917 5065 5310 1606 Size: 809 516 309 677 1148 245 2120 Fragments arranged by size: 2120 1148 809 677 516 309 245 NaeI GCC'GGC Cuts at: 2651 2651 Size: 5824 NcoI C'CATG_G Cuts at: 513 1065 2304 513 Size: 552 1239 4033 Fragments arranged by size: 4033 1239 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5824 NgoMIV G'CCGG_C Cuts at: 2649 2649 Size: 5824 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5824 NotI GC'GGCC_GC Cuts at: 3271 3271 Size: 5824 NspI r_CATG'y Cuts at: 1132 1229 2374 3121 3182 4727 1132 Size: 97 1145 747 61 1545 2229 Fragments arranged by size: 2229 1545 1145 747 97 61 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5824 PciI A'CATG_T Cuts at: 2370 4723 2370 Size: 2353 3471 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5824 PfoI T'CCnGG_A Cuts at: 2535 2535 Size: 5824 PmeI GTTT'AAAC Cuts at: 3128 3128 Size: 5824 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3893 3925 5406 5438 3893 Size: 32 1481 32 4279 Fragments arranged by size: 4279 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2497 2497 Size: 5824 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5824 PsiI TTA'TAA Cuts at: 3394 3394 Size: 5824 PstI C_TGCA'G Cuts at: 830 2748 3176 830 Size: 1918 428 3478 Fragments arranged by size: 3478 1918 428 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5824 PvuII CAG'CTG Cuts at: 2931 2931 Size: 5824 SacI G_AGCT'C Cuts at: 2856 2856 Size: 5824 SalI G'TCGA_C Cuts at: 1936 3166 1936 Size: 1230 4594 SbfI CC_TGCA'GG Cuts at: 3176 3176 Size: 5824 ScaI AGT'ACT Cuts at: 4072 5784 4072 Size: 1712 4112 SfcI C'TryA_G Cuts at: 826 1045 2143 2744 3172 4307 4988 5179 826 Size: 219 1098 601 428 1135 681 191 1471 Fragments arranged by size: 1471 1135 1098 681 601 428 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5824 SmaI CCC'GGG Cuts at: 1872 3151 1872 Size: 1279 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2512 3928 4829 5091 820 Size: 197 926 569 1416 901 262 1553 Fragments arranged by size: 1553 1416 926 901 569 262 197 SnaBI TAC'GTA Cuts at: 493 3615 493 Size: 3122 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5824 SphI G_CATG'C Cuts at: 1132 3121 3182 1132 Size: 1989 61 3774 Fragments arranged by size: 3774 1989 61 SspI AAT'ATT Cuts at: 5 52 3748 5 Size: 47 3696 2081 Fragments arranged by size: 3696 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5824 StyI C'CwwG_G Cuts at: 513 1065 2103 2304 3263 513 Size: 552 1038 201 959 3074 Fragments arranged by size: 3074 1038 959 552 201 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3851 3868 4021 4206 4651 325 Size: 1255 2271 17 153 185 445 1498 Fragments arranged by size: 2271 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4070 5782 96 Size: 274 80 33 51 1443 2093 1712 138 Fragments arranged by size: 2093 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2829 2861 5784 5816 2829 Size: 32 2923 32 2837 Fragments arranged by size: 2923 2837 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5824 XbaI T'CTAG_A Cuts at: 3160 3160 Size: 5824 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2083 2128 2083 Size: 45 5779 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5824 XmaI C'CCGG_G Cuts at: 1870 3149 1870 Size: 1279 4545 XmnI GAAnn'nnTTC Cuts at: 2777 3953 2777 Size: 1176 4648 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3632 276 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI