Restriction map for
pFN21AB6006
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# NO CUT ENZYMES ( 71 enzymes) #
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AatII AccII AccIII AclI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AvrII BbeI BciVI BfrI
BlnI BlpI Bpu1102I BseAI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII Bst1107I BstBI BstUI BstZ17I
CelII ClaI CpoI Eco47III FseI
HindIII HpaI KasI KspI MfeI
MluI MroI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PinAI PmeI Psp1406I
PvuI RsrII SacII SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SspBI SwaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AseI 6304 ATTAAT AT^TAAT
AsnI 6304 ATTAAT AT^TAAT
AviII 1670 TGCGCA TGC^GCA
BamHI 4213 GGATCC G^GATCC
BsaBI 5140 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 439 ACCGGA [AT]^CCGG[AT]
BsrBI 3544 CCGCTC CCGCTC(-3/-3)
BsrDI 5963 GCAATG GCAATG(2/0)
BsrFI 2791 GCCGGT [GA]^CCGG[CT]
BstAPI 1432 GCAAACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3900 GGTGACC G^GT[ACGT]ACC
BstPI 3900 GGTGACC G^GT[ACGT]ACC
Cfr10I 2791 GCCGGT [GA]^CCGG[CT]
DraIII 5591 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 6518 CGGCCG C^GGCCG
EclXI 6518 CGGCCG C^GGCCG
Eco52I 6518 CGGCCG C^GGCCG
EcoO65I 3900 GGTGACC G^GT[ACGT]ACC
EcoT22I 2948 ATGCAT ATGCA^T
FspI 1670 TGCGCA TGC^GCA
HaeII 2893 AGCGCC [GA]GCGC^[CT]
MamI 5140 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 2948 ATGCAT ATGCA^T
PflMI 1236 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 6304 ATTAAT AT^TAAT
SpeI 4351 ACTAGT A^CTAGT
SspI 631 AATATT AAT^ATT
Van91I 1236 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 36 enzymes) #
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Acc65I 1327 GGTACC G^GTACC
7295 GGTACC
AcyI 63 GGCGTC G[GA]^CG[CT]C
3843 GACGCC
AflIII 2880 ACACGT A^C[GA][CT]GT
4622 ACACGT
AhdI 4156 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5662 GACTATGAGTC
Asp718I 1327 GGTACC G^GTACC
7295 GGTACC
AspEI 4156 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5662 GACTATGAGTC
AvaI 991 CTCGAG C^[CT]CG[GA]G
3416 CTCGAG
BbrPI 1717 CACGTG CAC^GTG
6932 CACGTG
BcgI 3147 CGACAAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
6982 GCATCCAAGTCG
BclI 335 TGATCA T^GATCA
380 TGATCA
BsaHI 63 GGCGTC G[GA]^CG[CT]C
3843 GACGCC
BsgI 6929 CTGCAC GTGCAG(16/14)
5018 GTGCAG
BsiEI 5 CGACCG CG[GA][CT]^CG
6518 CGGCCG
BsmBI 3419 GAGACG CGTCTC(1/5)
3451 GAGACG
BsoBI 991 CTCGAG C^[CT]CG[GA]G
3416 CTCGAG
BspHI 3255 TCATGA T^CATGA
7269 TCATGA
DraI 2262 TTTAAA TTT^AAA
4537 TTTAAA
Eam1105I 4156 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5662 GACTATGAGTC
EcoRV 3375 GATATC GAT^ATC
5347 GATATC
FauI 4200 CCCGC CCCGC(4/6)
6823 GCGGG
FbaI 335 TGATCA T^GATCA
380 TGATCA
Hin1I 63 GGCGTC G[GA]^CG[CT]C
3843 GACGCC
HincII 3145 GTCGAC GT[CT]^[GA]AC
4988 GTCGAC
HindII 3145 GTCGAC GT[CT]^[GA]AC
4988 GTCGAC
KpnI 1327 GGTACC GGTAC^C
7295 GGTACC
NdeI 4633 CATATG CA^TATG
5867 CATATG
PaeR7I 991 CTCGAG C^TCGAG
3416 CTCGAG
PmaCI 1717 CACGTG CAC^GTG
6932 CACGTG
PmlI 1717 CACGTG CAC^GTG
6932 CACGTG
RcaI 3255 TCATGA T^CATGA
7269 TCATGA
SalI 3145 GTCGAC G^TCGAC
4988 GTCGAC
SapI 1831 GAAGAGC GCTCTTC(1/4)
3762 GAAGAGC
SexAI 5592 ACCTGGT A^CC[AT]GGT
5635 ACCAGGT
SphI 4722 GCATGC GCATG^C
7387 GCATGC
XbaI 1521 TCTAGA T^CTAGA
5938 TCTAGA
XhoI 991 CTCGAG C^TCGAG
3416 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AspI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BglII BmyI BpmI
BpuAI BsaAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrI BssKI
BssSI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PleI PpuMI PshAI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XcmI XhoII XmnI