(Circular) (Six-base) MAPSORT of: pFN21AB6006.seq Check: 8768 from: 1 to: 12109 pFN21AB6006 12109 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2166 9244 2166 Size: 7078 5031 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 9919 278 Size: 53 83 186 936 8383 2468 Fragments arranged by size: 8383 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4764 5151 5369 5414 6994 9452 1937 Size: 2827 387 218 45 1580 2458 4594 Fragments arranged by size: 4594 2827 2458 1580 387 218 45 Acc65I G'GTAC_C Cuts at: 3332 9300 9430 3332 Size: 5968 130 6011 Fragments arranged by size: 6011 5968 130 AclI AA'CG_TT Cuts at: 1563 10236 10609 1563 Size: 8673 373 3063 Fragments arranged by size: 8673 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2301 2598 2789 3390 3557 3659 3789 3963 Size: 669 297 191 601 167 102 130 174 Cuts at: 3963 4266 4560 4625 4722 4797 5073 5103 5148 Size: 303 294 65 97 75 276 30 45 Cuts at: 5148 5586 5595 5712 6114 6591 6973 7080 7824 Size: 438 9 117 402 477 382 107 744 Cuts at: 7824 7875 8085 8172 8373 8523 8565 10172 11556 Size: 51 210 87 201 150 42 1607 1384 Cuts at: 11556 11824 1632 Size: 268 1917 Fragments arranged by size: 1917 1607 1384 744 669 601 477 438 402 382 303 297 294 276 268 210 201 191 174 167 150 130 117 107 102 97 87 75 65 51 45 42 30 9 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 11912 AflIII A'CryG_T Cuts at: 1203 4885 6627 11008 11985 1203 Size: 3682 1742 4381 977 1327 Fragments arranged by size: 4381 3682 1742 1327 977 AgeI A'CCGG_T Cuts at: 10940 10940 Size: 12109 AhdI GACnn_n'nnGTC Cuts at: 6166 7672 10838 6166 Size: 1506 3166 7437 Fragments arranged by size: 7437 3166 1506 AleI CACnn'nnGTG Cuts at: 1166 2313 2372 3587 3713 4890 1166 Size: 1147 59 1215 126 1177 8385 Fragments arranged by size: 8385 1215 1177 1147 126 59 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2390 3320 3689 6993 9266 1184 Size: 1206 930 369 3304 2273 4027 Fragments arranged by size: 4027 3304 2273 1206 930 369 AlwNI CAG_nnn'CTG Cuts at: 3547 3812 4385 4424 4820 4847 5168 6341 6782 Size: 265 573 39 396 27 321 1173 441 Cuts at: 6782 8695 9852 11424 3547 Size: 1913 1157 1572 4232 Fragments arranged by size: 4232 1913 1572 1173 1157 573 441 396 321 265 39 27 ApaLI G'TGCA_C Cuts at: 10166 11322 10166 Size: 1156 10953 ApoI r'AATT_y Cuts at: 1493 3353 4778 5339 5477 5654 6095 6947 9128 Size: 1860 1425 561 138 177 441 852 2181 Cuts at: 9128 9418 9644 9796 11905 1493 Size: 290 226 152 2109 1697 Fragments arranged by size: 2181 2109 1860 1697 1425 852 561 441 290 226 177 152 138 AseI AT'TA_AT Cuts at: 160 1032 8310 10663 160 Size: 872 7278 2353 1606 Fragments arranged by size: 7278 2353 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12109 AvaI C'yCGr_G Cuts at: 1870 1943 2996 5421 9434 1870 Size: 73 1053 2425 4013 4545 Fragments arranged by size: 4545 4013 2425 1053 73 BamHI G'GATC_C Cuts at: 6218 9439 9806 6218 Size: 3221 367 8521 Fragments arranged by size: 8521 3221 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3332 3577 5174 6275 9300 Size: 325 217 627 1545 245 1597 1101 3025 Cuts at: 9300 9430 10885 618 Size: 130 1455 1842 Fragments arranged by size: 3025 1842 1597 1545 1455 1101 627 325 245 217 130 BanII G_rGCy'C Cuts at: 1391 2187 2454 2997 3020 4746 5227 5394 5774 Size: 796 267 543 23 1726 481 167 380 Cuts at: 5774 6153 6686 7164 7491 8392 9195 9428 1391 Size: 379 533 478 327 901 803 233 4072 Fragments arranged by size: 4072 1726 901 803 796 543 533 481 478 380 379 327 267 233 167 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3917 3951 4596 5016 5136 5331 5655 Size: 971 2018 34 645 420 120 195 324 Cuts at: 5655 6420 6555 6630 7598 7691 7844 8553 8735 Size: 765 135 75 968 93 153 709 182 Cuts at: 8735 9141 928 Size: 406 3896 Fragments arranged by size: 3896 2018 971 968 765 709 645 420 406 324 195 182 153 135 120 93 75 34 BbvCI CC'TCA_GC Cuts at: 4572 4857 6732 9186 4572 Size: 285 1875 2454 7495 Fragments arranged by size: 7495 2454 1875 285 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5141 5175 8976 9010 10300 10334 1903 Size: 34 3204 34 3801 34 1290 34 3678 Fragments arranged by size: 3801 3678 3204 1290 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 10001 11211 10001 Size: 1210 10899 BclI T'GATC_A Cuts at: 1548 2340 2385 1548 Size: 792 45 11272 Fragments arranged by size: 11272 792 45 BfrBI ATG'CAT Cuts at: 4955 4955 Size: 12109 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2063 3863 4382 Size: 107 122 71 631 605 391 1800 519 Cuts at: 4382 6674 10720 136 Size: 2292 4046 1525 Fragments arranged by size: 4046 2292 1800 1525 631 605 519 391 122 107 71 BglII A'GATC_T Cuts at: 3382 5663 6920 8716 9328 12104 3382 Size: 2281 1257 1796 612 2776 3387 Fragments arranged by size: 3387 2776 2281 1796 1257 612 BlpI GC'TnA_GC Cuts at: 9526 9526 Size: 12109 Bme1580I G_kGCm'C Cuts at: 1790 3582 8513 9057 10170 11326 1790 Size: 1792 4931 544 1113 1156 2573 Fragments arranged by size: 4931 2573 1792 1156 1113 544 BmgBI CAC'GTC Cuts at: 1318 5428 1318 Size: 4110 7999 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3481 5221 9840 Size: 416 53 83 399 24 2064 1740 4619 Cuts at: 9840 10798 442 Size: 958 1753 Fragments arranged by size: 4619 2064 1753 1740 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 12109 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2719 5805 6757 7183 7352 7354 8530 2719 Size: 3086 952 426 169 2 1176 6298 Fragments arranged by size: 6298 3086 1176 952 426 169 2 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2444 3143 3831 4428 4598 4760 4842 5298 Size: 1205 699 688 597 170 162 82 456 Cuts at: 5298 6303 6987 7078 7592 8385 8592 10769 1239 Size: 1005 684 91 514 793 207 2177 2579 Fragments arranged by size: 2579 2177 1205 1005 793 699 688 684 597 514 456 207 170 162 91 82 Bpu10I CC'TnA_GC Cuts at: 4572 4857 6732 6966 9186 4572 Size: 285 1875 234 2220 7495 Fragments arranged by size: 7495 2220 1875 285 234 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3284 8345 10234 11099 11397 3284 Size: 5061 1889 865 298 3996 Fragments arranged by size: 5061 3996 1889 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3788 5435 7769 8954 9302 10772 882 Size: 617 2289 1647 2334 1185 348 1470 2219 Fragments arranged by size: 2334 2289 2219 1647 1470 1185 617 348 BsaAI yAC'GTr Cuts at: 493 1204 3724 4888 5675 6630 8586 8939 9160 Size: 711 2520 1164 787 955 1956 353 221 Cuts at: 9160 9900 493 Size: 740 2702 Fragments arranged by size: 2702 2520 1956 1164 955 787 740 711 353 221 BsaBI GATnn'nnATC Cuts at: 7149 12103 7149 Size: 4954 7155 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2069 5849 9916 10298 Size: 53 83 186 936 536 3780 4067 382 Cuts at: 10298 10933 275 Size: 635 1451 Fragments arranged by size: 4067 3780 1451 936 635 536 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2444 10542 10940 11214 11361 11772 2444 Size: 8098 398 274 147 411 2781 Fragments arranged by size: 8098 2781 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2604 2634 3038 3068 7115 Size: 30 165 30 1201 30 404 30 4047 Cuts at: 7115 7145 7732 7762 8902 8932 1178 Size: 30 587 30 1140 30 4355 Fragments arranged by size: 4355 4047 1201 1140 587 404 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2165 3971 4227 4748 4865 4874 5072 5084 Size: 979 1806 256 521 117 9 198 12 Cuts at: 5084 5111 5195 5327 7139 7224 7257 9203 1186 Size: 27 84 132 1812 85 33 1946 4092 Fragments arranged by size: 4092 1946 1812 1806 979 521 256 198 132 117 85 84 33 27 12 9 BseYI C'CCAG_C Cuts at: 3827 8015 9194 11312 11808 3827 Size: 4188 1179 2118 496 4128 Fragments arranged by size: 4188 4128 2118 1179 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 7044 8919 1163 Size: 573 5308 1875 4353 Fragments arranged by size: 5308 4353 1875 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 8526 9559 10320 11348 1540 Size: 275 186 12 6513 1033 761 1028 2301 Fragments arranged by size: 6513 2301 1033 1028 761 275 186 12 BsiHKAI G_wGCw'C Cuts at: 2157 2187 2454 2997 6686 7164 10170 10255 11326 Size: 30 267 543 3689 478 3006 85 1071 Cuts at: 11326 2157 Size: 2940 Fragments arranged by size: 3689 3006 2940 1071 543 478 267 85 30 BsmI GAATG_Cn' Cuts at: 2225 6845 8974 9620 9713 2225 Size: 4620 2129 646 93 4621 Fragments arranged by size: 4621 4620 2129 646 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5418 5450 5418 Size: 32 12077 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2157 2187 2454 2997 3020 3582 4746 Size: 399 367 30 267 543 23 562 1164 Cuts at: 4746 5227 5394 5774 6153 6686 7164 7491 8392 Size: 481 167 380 379 533 478 327 901 Cuts at: 8392 8513 9057 9195 9428 10170 10255 11326 1391 Size: 121 544 138 233 742 85 1071 2174 Fragments arranged by size: 2174 1164 1071 901 742 562 544 543 533 481 478 399 380 379 367 327 267 233 167 138 121 85 30 23 BspEI T'CCGG_A Cuts at: 11772 11772 Size: 12109 BspHI T'CATG_A Cuts at: 5260 9274 9998 11728 5260 Size: 4014 724 1730 5641 Fragments arranged by size: 5641 4014 1730 724 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2166 5254 5542 9244 9464 844 Size: 1322 3088 288 3702 220 3489 Fragments arranged by size: 3702 3489 3088 1322 288 220 BsrBI CCG'CTC Cuts at: 1394 1823 5551 9996 1394 Size: 429 3728 4445 3507 Fragments arranged by size: 4445 3728 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 7975 10604 10778 65 Size: 1183 6727 2629 174 1396 Fragments arranged by size: 6727 2629 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4796 10753 10940 1700 Size: 110 2986 5957 187 2869 Fragments arranged by size: 5957 2986 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 12109 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 12109 BssSI C'ACGA_G Cuts at: 2414 7371 8255 10169 11181 2414 Size: 4957 884 1914 1012 3342 Fragments arranged by size: 4957 3342 1914 1012 884 BstAPI GCAn_nnn'nTGC Cuts at: 794 3443 794 Size: 2649 9460 BstBI TT'CG_AA Cuts at: 10913 10913 Size: 12109 BstEII G'GTnAC_C Cuts at: 1184 5905 1184 Size: 4721 7388 BstXI CCAn_nnnn'nTGG Cuts at: 3589 5234 5401 8006 3589 Size: 1645 167 2605 7692 Fragments arranged by size: 7692 2605 1645 167 BstYI r'GATC_y Cuts at: 1972 2298 3382 3562 4507 5663 6218 6536 6920 Size: 326 1084 180 945 1156 555 318 384 Cuts at: 6920 8716 9328 9439 9806 10191 10208 11660 11746 Size: 1796 612 111 367 385 17 1452 86 Cuts at: 11746 11758 12104 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1796 1452 1156 1084 945 612 555 385 384 367 346 326 318 180 111 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3702 4503 4731 5334 5901 5943 7629 8160 8922 Size: 801 228 603 567 42 1686 531 762 Cuts at: 8922 3702 Size: 6889 Fragments arranged by size: 6889 1686 801 762 603 567 531 228 42 BtgI C'CryG_G Cuts at: 513 1065 2004 2013 5400 5640 6141 8658 513 Size: 552 939 9 3387 240 501 2517 3964 Fragments arranged by size: 3964 3387 2517 939 552 501 240 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2038 487 Size: 683 56 812 10558 Fragments arranged by size: 10558 812 683 56 BtsI GCAGTG_nn' Cuts at: 2435 2838 3329 5343 5998 8202 9009 9625 10419 Size: 403 491 2014 655 2204 807 616 794 Cuts at: 10419 10439 2435 Size: 20 4105 Fragments arranged by size: 4105 2204 2014 807 794 655 616 491 403 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 12109 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 7044 7079 529 Size: 35 6480 35 5559 Fragments arranged by size: 6480 5559 35 35 DraI TTT'AAA Cuts at: 4269 6544 9413 4269 Size: 2275 2869 6965 Fragments arranged by size: 6965 2869 2275 DraIII CAC_nnn'GTG Cuts at: 7601 7601 Size: 12109 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7939 8579 9040 11116 809 Size: 507 6623 640 461 2076 1802 Fragments arranged by size: 6623 2076 1802 640 507 461 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3549 3571 4983 Size: 54 1425 50 275 18 1719 22 1412 Cuts at: 4983 5236 6421 6952 7996 8523 8679 8997 9556 Size: 253 1185 531 1044 527 156 318 559 Cuts at: 9556 10445 12074 8 Size: 889 1629 43 Fragments arranged by size: 1719 1629 1425 1412 1185 1044 889 559 531 527 318 275 253 156 54 50 43 22 18 EagI C'GGCC_G Cuts at: 1537 1812 8523 9556 1537 Size: 275 6711 1033 4090 Fragments arranged by size: 6711 4090 1033 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3122 3831 4032 4071 4146 4287 4521 4536 Size: 1769 709 201 39 75 141 234 15 Cuts at: 4536 4590 4827 4863 4964 5049 5448 5641 5762 Size: 54 237 36 101 85 399 193 121 Cuts at: 5762 7207 7462 7851 8499 8940 10039 1353 Size: 1445 255 389 648 441 1099 3423 Fragments arranged by size: 3423 1769 1445 1099 709 648 441 399 389 255 237 234 201 193 141 121 101 85 75 54 39 36 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2877 4958 10696 11070 11216 387 Size: 1064 138 1288 2081 5738 374 146 1280 Fragments arranged by size: 5738 2081 1288 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2185 2452 2995 6684 7162 2185 Size: 267 543 3689 478 7132 Fragments arranged by size: 7132 3689 543 478 267 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2301 2444 2598 2789 3143 3390 3557 Size: 393 669 143 154 191 354 247 167 Cuts at: 3557 3659 3789 3831 3963 4266 4428 4560 4598 Size: 102 130 42 132 303 162 132 38 Cuts at: 4598 4625 4722 4760 4797 4842 5073 5103 5148 Size: 27 97 38 37 45 231 30 45 Cuts at: 5148 5298 5586 5595 5712 6114 6303 6591 6973 Size: 150 288 9 117 402 189 288 382 Cuts at: 6973 6987 7078 7080 7592 7824 7875 8085 8172 Size: 14 91 2 512 232 51 210 87 Cuts at: 8172 8373 8385 8523 8565 8592 10172 10769 11556 Size: 201 12 138 42 27 1580 597 787 Cuts at: 11556 11824 1239 Size: 268 1524 Fragments arranged by size: 1580 1524 787 669 597 512 402 393 382 354 303 288 288 268 247 232 231 210 201 191 189 167 162 154 150 143 138 132 132 130 117 102 97 91 87 51 45 45 42 42 38 38 37 30 27 27 14 12 9 2 EcoNI CCTnn'n_nnAGG Cuts at: 2580 3063 7574 8403 2580 Size: 483 4511 829 6286 Fragments arranged by size: 6286 4511 829 483 EcoO109I rG'GnC_Cy Cuts at: 4485 4664 7644 8011 4485 Size: 179 2980 367 8583 Fragments arranged by size: 8583 2980 367 179 EcoRI G'AATT_C Cuts at: 3353 5339 5477 6095 9418 9796 3353 Size: 1986 138 618 3323 378 5666 Fragments arranged by size: 5666 3323 1986 618 378 138 EcoRV GAT'ATC Cuts at: 5382 7354 5382 Size: 1972 10137 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2134 2134 Size: 12109 FspI TGC'GCA Cuts at: 3677 10615 3677 Size: 6938 5171 HaeII r_GCGC'y Cuts at: 1678 4902 11256 1678 Size: 3224 6354 2531 Fragments arranged by size: 6354 3224 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2283 2290 2315 2322 2604 Size: 32 135 32 711 7 25 7 282 Cuts at: 2604 2636 2861 2893 5396 5428 5447 5479 5717 Size: 32 225 32 2503 32 19 32 238 Cuts at: 5717 5749 6020 6052 6152 6184 6617 6649 6698 Size: 32 271 32 100 32 433 32 49 Cuts at: 6698 6730 6905 6937 7115 7147 7216 7238 7248 Size: 32 175 32 178 32 69 22 10 Cuts at: 7248 7270 7400 7432 7463 7495 7658 7690 8780 Size: 22 130 32 31 32 163 32 1090 Cuts at: 8780 8812 8840 8872 9256 9288 10750 10782 10824 Size: 32 28 32 384 32 1462 32 42 Cuts at: 10824 10856 1373 Size: 32 2626 Fragments arranged by size: 2626 2503 1462 1090 711 433 384 282 271 238 225 178 175 163 135 130 100 69 49 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 31 28 25 22 22 19 10 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 5152 6995 9453 9699 669 Size: 988 281 3214 1843 2458 246 3079 Fragments arranged by size: 3214 3079 2458 1843 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 12109 HpaI GTT'AAC Cuts at: 9699 9699 Size: 12109 KpnI G_GTAC'C Cuts at: 3336 9304 9434 3336 Size: 5968 130 6011 Fragments arranged by size: 6011 5968 130 MfeI C'AATT_G Cuts at: 9708 9708 Size: 12109 MluI A'CGCG_T Cuts at: 11985 11985 Size: 12109 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2923 3489 8535 9201 11223 11407 1305 Size: 1618 566 5046 666 2022 184 2007 Fragments arranged by size: 5046 2022 2007 1618 666 566 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3551 3573 4985 6423 6954 Size: 54 1425 343 1719 22 1412 1438 531 Cuts at: 6954 7998 8681 10 Size: 1044 683 3438 Fragments arranged by size: 3438 1719 1438 1425 1412 1044 683 531 343 54 22 MslI CAynn'nnrTG Cuts at: 518 1166 2313 2372 3122 3587 3713 4890 5399 Size: 648 1147 59 750 465 126 1177 509 Cuts at: 5399 6557 8649 9254 10067 10426 10585 518 Size: 1158 2092 605 813 359 159 2042 Fragments arranged by size: 2092 2042 1177 1158 1147 813 750 648 605 509 465 359 159 126 59 MspA1I CmG'CkG Cuts at: 1606 2980 4262 4397 4424 4439 4576 5738 8828 Size: 1374 1282 135 27 15 137 1162 3090 Cuts at: 8828 9525 10202 11350 11595 1606 Size: 697 677 1148 245 2120 Fragments arranged by size: 3090 2120 1374 1282 1162 1148 697 677 245 137 135 27 15 NcoI C'CATG_G Cuts at: 513 1065 2004 5400 5640 6141 8658 513 Size: 552 939 3396 240 501 2517 3964 Fragments arranged by size: 3964 3396 2517 939 552 501 240 NdeI CA'TA_TG Cuts at: 387 6639 7873 387 Size: 6252 1234 4623 Fragments arranged by size: 6252 4623 1234 NheI G'CTAG_C Cuts at: 1052 1052 Size: 12109 NotI GC'GGCC_GC Cuts at: 9556 9556 Size: 12109 NsiI A_TGCA'T Cuts at: 4957 4957 Size: 12109 NspI r_CATG'y Cuts at: 1132 1229 2974 6731 7087 9253 9396 9467 11012 Size: 97 1745 3757 356 2166 143 71 1545 Cuts at: 11012 1132 Size: 2229 Fragments arranged by size: 3757 2229 2166 1745 1545 356 143 97 71 PasI CC'CwG_GG Cuts at: 1348 3013 5818 1348 Size: 1665 2805 7639 Fragments arranged by size: 7639 2805 1665 PciI A'CATG_T Cuts at: 11008 11008 Size: 12109 PflMI CCAn_nnn'nTGG Cuts at: 1108 3247 1108 Size: 2139 9970 PfoI T'CCnGG_A Cuts at: 2800 3498 6807 6896 8401 9207 2800 Size: 698 3309 89 1505 806 5702 Fragments arranged by size: 5702 3309 1505 806 698 89 PmeI GTTT'AAAC Cuts at: 9413 9413 Size: 12109 PmlI CAC'GTG Cuts at: 3724 8939 3724 Size: 5215 6894 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 10178 10210 11691 11723 10178 Size: 32 1481 32 10564 Fragments arranged by size: 10564 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4485 4664 7644 8011 4485 Size: 179 2980 367 8583 Fragments arranged by size: 8583 2980 367 179 PshAI GACnn'nnGTC Cuts at: 1613 6853 6990 7071 1613 Size: 5240 137 81 6651 Fragments arranged by size: 6651 5240 137 81 PsiI TTA'TAA Cuts at: 9679 9679 Size: 12109 PspXI vC'TCGA_Gb Cuts at: 2996 2996 Size: 12109 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 8095 8127 8095 Size: 32 12077 PstI C_TGCA'G Cuts at: 830 4396 5164 5875 7423 8140 8209 9016 9461 Size: 3566 768 711 1548 717 69 807 445 Cuts at: 9461 830 Size: 3478 Fragments arranged by size: 3566 3478 1548 807 768 717 711 445 69 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 12109 PvuII CAG'CTG Cuts at: 4262 4397 4424 4439 4576 5738 4262 Size: 135 27 15 137 1162 10633 Fragments arranged by size: 10633 1162 137 135 27 15 SacI G_AGCT'C Cuts at: 2187 2454 2997 6686 7164 2187 Size: 267 543 3689 478 7132 Fragments arranged by size: 7132 3689 543 478 267 SalI G'TCGA_C Cuts at: 1936 5150 6993 9451 1936 Size: 3214 1843 2458 4594 Fragments arranged by size: 4594 3214 2458 1843 SanDI GG'GwC_CC Cuts at: 8011 8011 Size: 12109 SapI GCTCTTCn'nnn_ Cuts at: 3831 5762 3831 Size: 1931 10178 SbfI CC_TGCA'GG Cuts at: 9461 9461 Size: 12109 ScaI AGT'ACT Cuts at: 10357 12069 10357 Size: 1712 10397 SexAI A'CCwGG_T Cuts at: 7597 7640 7597 Size: 43 12066 SfcI C'TryA_G Cuts at: 826 1045 4392 5160 5871 6051 7014 7069 7419 Size: 219 3347 768 711 180 963 55 350 Cuts at: 7419 8136 8205 9012 9457 10592 11273 11464 826 Size: 717 69 807 445 1135 681 191 1471 Fragments arranged by size: 3347 1471 1135 963 807 768 717 711 681 445 350 219 191 180 69 55 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12109 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 12109 SmaI CCC'GGG Cuts at: 1872 9436 1872 Size: 7564 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2996 3263 5421 8324 10213 11114 Size: 197 926 1053 267 2158 2903 1889 901 Cuts at: 11114 11376 820 Size: 262 1553 Fragments arranged by size: 2903 2158 1889 1553 1053 926 901 267 262 197 SnaBI TAC'GTA Cuts at: 493 9900 493 Size: 9407 2702 SpeI A'CTAG_T Cuts at: 152 6356 152 Size: 6204 5905 SphI G_CATG'C Cuts at: 1132 6731 9396 9467 1132 Size: 5599 2665 71 3774 Fragments arranged by size: 5599 3774 2665 71 SspI AAT'ATT Cuts at: 5 52 2638 10033 5 Size: 47 2586 7395 2081 Fragments arranged by size: 7395 2586 2081 47 StuI AGG'CCT Cuts at: 1517 7187 8675 9063 1517 Size: 5670 1488 388 4563 Fragments arranged by size: 5670 4563 1488 388 StyI C'CwwG_G Cuts at: 513 1065 2004 3903 4515 4938 5400 5640 6141 Size: 552 939 1899 612 423 462 240 501 Cuts at: 6141 8658 8676 8805 9548 513 Size: 2517 18 129 743 3074 Fragments arranged by size: 3074 2517 1899 939 743 612 552 501 462 423 240 129 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2162 2552 2925 2948 3500 3569 3751 Size: 1255 582 390 373 23 552 69 182 Cuts at: 3751 5013 5810 6865 7010 7154 7697 8492 10136 Size: 1262 797 1055 145 144 543 795 1644 Cuts at: 10136 10153 10306 10491 10936 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1644 1498 1262 1255 1055 797 795 582 552 543 445 390 373 185 182 153 145 144 69 23 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3489 10355 12067 Size: 274 80 33 51 1443 1512 6866 1712 Cuts at: 12067 96 Size: 138 Fragments arranged by size: 6866 1712 1512 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2524 2556 4648 4680 7732 7764 8902 8934 12069 Size: 32 2092 32 3052 32 1138 32 3135 Cuts at: 12069 12101 2524 Size: 32 2532 Fragments arranged by size: 3135 3052 2532 2092 1138 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5461 8761 9040 1316 Size: 4145 3300 279 4385 Fragments arranged by size: 4385 4145 3300 279 XbaI T'CTAG_A Cuts at: 3526 7943 9445 3526 Size: 4417 1502 6190 Fragments arranged by size: 6190 4417 1502 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 5710 6670 7567 2011 Size: 3699 960 897 6553 Fragments arranged by size: 6553 3699 960 897 XhoI C'TCGA_G Cuts at: 1943 2996 5421 1943 Size: 1053 2425 8631 Fragments arranged by size: 8631 2425 1053 XmaI C'CCGG_G Cuts at: 1870 9434 1870 Size: 7564 4545 XmnI GAAnn'nnTTC Cuts at: 2840 3357 6240 10238 2840 Size: 517 2883 3998 4711 Fragments arranged by size: 4711 3998 2883 517 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 9917 276 Size: 53 83 186 936 8383 2468 Fragments arranged by size: 8383 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI RsrII SacII SfiI SfoI SrfI SwaI