(Circular) (Six-base) MAPSORT of: pFN21AB5685.seq Check: 8955 from: 1 to: 5911 pFN21AB5685 5911 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2036 2128 2036 Size: 92 5819 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3721 278 Size: 53 83 186 936 2185 2468 Fragments arranged by size: 2468 2185 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2925 3254 1937 Size: 988 329 4594 Fragments arranged by size: 4594 988 329 Acc65I G'GTAC_C Cuts at: 3232 3232 Size: 5911 AclI AA'CG_TT Cuts at: 1563 4038 4411 1563 Size: 2475 373 3063 Fragments arranged by size: 3063 2475 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2398 2402 2940 3974 5358 5626 1632 Size: 766 4 538 1034 1384 268 1917 Fragments arranged by size: 1917 1384 1034 766 538 268 4 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5714 AflIII A'CryG_T Cuts at: 1203 2964 4810 5787 1203 Size: 1761 1846 977 1327 Fragments arranged by size: 1846 1761 1327 977 AgeI A'CCGG_T Cuts at: 4742 4742 Size: 5911 AhdI GACnn_n'nnGTC Cuts at: 4640 4640 Size: 5911 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5911 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5911 AlwNI CAG_nnn'CTG Cuts at: 2856 3034 3654 5226 2856 Size: 178 620 1572 3541 Fragments arranged by size: 3541 1572 620 178 ApaLI G'TGCA_C Cuts at: 2075 3968 5124 2075 Size: 1893 1156 2862 Fragments arranged by size: 2862 1893 1156 ApoI r'AATT_y Cuts at: 1493 3220 3446 3598 5707 1493 Size: 1727 226 152 2109 1697 Fragments arranged by size: 2109 1727 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4465 160 Size: 872 3433 1606 Fragments arranged by size: 3433 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 AvaI C'yCGr_G Cuts at: 1870 1943 3119 3236 1870 Size: 73 1176 117 4545 Fragments arranged by size: 4545 1176 117 73 BamHI G'GATC_C Cuts at: 3241 3608 3241 Size: 367 5544 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2806 3232 4687 618 Size: 325 217 627 1019 426 1455 1842 Fragments arranged by size: 1842 1455 1019 627 426 325 217 BanII G_rGCy'C Cuts at: 1391 2319 3230 1391 Size: 928 911 4072 Fragments arranged by size: 4072 928 911 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2501 2606 928 Size: 971 602 105 4233 Fragments arranged by size: 4233 971 602 105 BbvCI CC'TCA_GC Cuts at: 2771 2771 Size: 5911 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4102 4136 1903 Size: 34 50 34 2081 34 3678 Fragments arranged by size: 3678 2081 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3803 5013 3803 Size: 1210 4701 BclI T'GATC_A Cuts at: 1548 2514 1548 Size: 966 4945 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2105 3113 4522 Size: 107 122 71 631 605 433 1008 1409 Cuts at: 4522 136 Size: 1525 Fragments arranged by size: 1525 1409 1008 631 605 433 122 107 71 BglII A'GATC_T Cuts at: 5906 5906 Size: 5911 BlpI GC'TnA_GC Cuts at: 3328 3328 Size: 5911 Bme1580I G_kGCm'C Cuts at: 1790 2079 2673 2842 3972 5128 1790 Size: 289 594 169 1130 1156 2573 Fragments arranged by size: 2573 1156 1130 594 289 169 BmgBI CAC'GTC Cuts at: 1318 3022 1318 Size: 1704 4207 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2497 3642 4600 Size: 416 53 83 399 24 1080 1145 958 Cuts at: 4600 442 Size: 1753 Fragments arranged by size: 1753 1145 1080 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5911 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2001 2195 2602 2933 4571 1239 Size: 762 194 407 331 1638 2579 Fragments arranged by size: 2579 1638 762 407 331 194 Bpu10I CC'TnA_GC Cuts at: 2771 2771 Size: 5911 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4036 4901 5199 4036 Size: 865 298 4748 Fragments arranged by size: 4748 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4574 882 Size: 617 3075 2219 Fragments arranged by size: 3075 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2095 3702 493 Size: 711 891 1607 2702 Fragments arranged by size: 2702 1607 891 711 BsaBI GATnn'nnATC Cuts at: 5905 5905 Size: 5911 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3718 4100 4735 275 Size: 53 83 186 936 2185 382 635 1451 Fragments arranged by size: 2185 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2424 4344 4742 5016 5163 5574 2424 Size: 1920 398 274 147 411 2761 Fragments arranged by size: 2761 1920 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5686 Fragments arranged by size: 5686 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2241 3070 3137 1186 Size: 1055 829 67 3960 Fragments arranged by size: 3960 1055 829 67 BseYI C'CCAG_C Cuts at: 2235 2841 5114 5610 2235 Size: 606 2273 496 2536 Fragments arranged by size: 2536 2273 606 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5338 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3361 4122 5150 1540 Size: 275 186 1360 761 1028 2301 Fragments arranged by size: 2301 1360 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2079 2319 2432 3972 4057 5128 2079 Size: 240 113 1540 85 1071 2862 Fragments arranged by size: 2862 1540 1071 240 113 85 BsmI GAATG_Cn' Cuts at: 2578 3422 3515 2578 Size: 844 93 4974 Fragments arranged by size: 4974 844 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2319 2432 2673 2842 3230 3972 Size: 399 289 240 113 241 169 388 742 Cuts at: 3972 4057 5128 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 388 289 241 240 169 113 85 BspEI T'CCGG_A Cuts at: 2424 5574 2424 Size: 3150 2761 BspHI T'CATG_A Cuts at: 3800 5530 3800 Size: 1730 4181 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2036 2128 3266 844 Size: 1192 92 1138 3489 Fragments arranged by size: 3489 1192 1138 92 BsrBI CCG'CTC Cuts at: 1394 1823 3798 1394 Size: 429 1975 3507 Fragments arranged by size: 3507 1975 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2781 4406 4580 65 Size: 1183 1533 1625 174 1396 Fragments arranged by size: 1625 1533 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4555 4742 1700 Size: 110 2745 187 2869 Fragments arranged by size: 2869 2745 187 110 BsrGI T'GTAC_A Cuts at: 96 2307 96 Size: 2211 3700 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5911 BssSI C'ACGA_G Cuts at: 2992 3971 4983 2992 Size: 979 1012 3920 Fragments arranged by size: 3920 1012 979 BstAPI GCAn_nnn'nTGC Cuts at: 794 3034 794 Size: 2240 3671 BstBI TT'CG_AA Cuts at: 4715 4715 Size: 5911 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5911 BstYI r'GATC_y Cuts at: 1972 2377 3241 3608 3993 4010 5462 5548 5560 Size: 405 864 367 385 17 1452 86 12 Cuts at: 5560 5906 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 864 405 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2930 2930 Size: 5911 BtgI C'CryG_G Cuts at: 513 1065 2327 2541 2758 513 Size: 552 1262 214 217 3666 Fragments arranged by size: 3666 1262 552 217 214 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5172 Fragments arranged by size: 5172 683 56 BtsI GCAGTG_nn' Cuts at: 3427 4221 4241 3427 Size: 794 20 5097 Fragments arranged by size: 5097 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5911 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5876 DraI TTT'AAA Cuts at: 3215 3215 Size: 5911 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4918 809 Size: 507 3602 1802 Fragments arranged by size: 3602 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2202 2590 2725 Size: 54 1425 50 275 18 372 388 135 Cuts at: 2725 3358 4247 5876 8 Size: 633 889 1629 43 Fragments arranged by size: 1629 1425 889 633 388 372 275 135 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3358 1537 Size: 275 1546 4090 Fragments arranged by size: 4090 1546 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2642 3841 1353 Size: 1289 1199 3423 Fragments arranged by size: 3423 1289 1199 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4498 4872 5018 387 Size: 1064 138 2909 374 146 1280 Fragments arranged by size: 2909 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2317 2317 Size: 5911 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2001 2195 2398 2402 2602 2933 2940 Size: 393 369 194 203 4 200 331 7 Cuts at: 2940 3974 4571 5358 5626 1239 Size: 1034 597 787 268 1524 Fragments arranged by size: 1524 1034 787 597 393 369 331 268 203 200 194 7 4 EcoRI G'AATT_C Cuts at: 3220 3598 3220 Size: 378 5533 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2623 2623 Size: 5911 FspI TGC'GCA Cuts at: 4417 4417 Size: 5911 HaeII r_GCGC'y Cuts at: 1678 3035 5058 1678 Size: 1357 2023 2531 Fragments arranged by size: 2531 2023 1357 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2463 2495 3132 3164 4552 Size: 32 135 32 891 32 637 32 1388 Cuts at: 4552 4584 4626 4658 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1388 891 637 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2926 3255 3501 669 Size: 988 281 988 329 246 3079 Fragments arranged by size: 3079 988 988 329 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5911 HpaI GTT'AAC Cuts at: 3501 3501 Size: 5911 KpnI G_GTAC'C Cuts at: 3236 3236 Size: 5911 MfeI C'AATT_G Cuts at: 3510 3510 Size: 5911 MluI A'CGCG_T Cuts at: 5787 5787 Size: 5911 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5025 5209 1305 Size: 3720 184 2007 Fragments arranged by size: 3720 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2204 2727 10 Size: 54 1425 343 372 523 3194 Fragments arranged by size: 3194 1425 523 372 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3869 4228 4387 518 Size: 648 843 1860 359 159 2042 Fragments arranged by size: 2042 1860 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2215 2374 2551 3092 3327 4004 5152 5397 Size: 609 159 177 541 235 677 1148 245 Cuts at: 5397 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 609 541 245 235 177 159 NcoI C'CATG_G Cuts at: 513 1065 2327 513 Size: 552 1262 4097 Fragments arranged by size: 4097 1262 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5911 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5911 NotI GC'GGCC_GC Cuts at: 3358 3358 Size: 5911 NspI r_CATG'y Cuts at: 1132 1229 2779 2968 3269 4814 1132 Size: 97 1550 189 301 1545 2229 Fragments arranged by size: 2229 1550 1545 301 189 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5911 PciI A'CATG_T Cuts at: 2964 4810 2964 Size: 1846 4065 PflMI CCAn_nnn'nTGG Cuts at: 1108 3202 1108 Size: 2094 3817 PfoI T'CCnGG_A Cuts at: 2742 2742 Size: 5911 PmeI GTTT'AAAC Cuts at: 3215 3215 Size: 5911 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3980 4012 5493 5525 3980 Size: 32 1481 32 4366 Fragments arranged by size: 4366 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2921 1613 Size: 1308 4603 PsiI TTA'TAA Cuts at: 3481 3481 Size: 5911 PstI C_TGCA'G Cuts at: 830 3039 3263 830 Size: 2209 224 3478 Fragments arranged by size: 3478 2209 224 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5911 PvuII CAG'CTG Cuts at: 2215 2551 3092 2215 Size: 336 541 5034 Fragments arranged by size: 5034 541 336 SacI G_AGCT'C Cuts at: 2319 2319 Size: 5911 SalI G'TCGA_C Cuts at: 1936 2924 3253 1936 Size: 988 329 4594 Fragments arranged by size: 4594 988 329 SbfI CC_TGCA'GG Cuts at: 3263 3263 Size: 5911 ScaI AGT'ACT Cuts at: 4159 5871 4159 Size: 1712 4199 SfcI C'TryA_G Cuts at: 826 1045 3035 3259 4394 5075 5266 826 Size: 219 1990 224 1135 681 191 1471 Fragments arranged by size: 1990 1471 1135 681 224 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2105 2105 Size: 5911 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5911 SmaI CCC'GGG Cuts at: 1872 3238 1872 Size: 1366 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4015 4916 5178 820 Size: 197 926 2072 901 262 1553 Fragments arranged by size: 2072 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3702 493 Size: 3209 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5911 SphI G_CATG'C Cuts at: 1132 3269 1132 Size: 2137 3774 SspI AAT'ATT Cuts at: 5 52 3835 5 Size: 47 3783 2081 Fragments arranged by size: 3783 2081 47 StuI AGG'CCT Cuts at: 1517 2150 1517 Size: 633 5278 StyI C'CwwG_G Cuts at: 513 1065 2154 2327 3350 513 Size: 552 1089 173 1023 3074 Fragments arranged by size: 3074 1089 1023 552 173 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2479 2639 2975 3938 3955 4108 4293 Size: 1255 899 160 336 963 17 153 185 Cuts at: 4293 4738 325 Size: 445 1498 Fragments arranged by size: 1498 1255 963 899 445 336 185 160 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2307 4157 5869 Size: 274 80 33 51 1443 330 1850 1712 Cuts at: 5869 96 Size: 138 Fragments arranged by size: 1850 1712 1443 330 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5871 5903 5871 Size: 32 5879 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5911 XbaI T'CTAG_A Cuts at: 3247 3247 Size: 5911 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2908 2908 Size: 5911 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5911 XmaI C'CCGG_G Cuts at: 1870 3236 1870 Size: 1366 4545 XmnI GAAnn'nnTTC Cuts at: 4040 4040 Size: 5911 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3719 276 Size: 53 83 186 936 2185 2468 Fragments arranged by size: 2468 2185 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI