Restriction map for
pFN21AB5677
##################################
# NO CUT ENZYMES ( 166 enzymes) #
##################################
AatII AccI AccII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
Asp700I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BciVI BfaI
BfrI BglI BlnI BlpI BmyI
Bpu1102I BsaAI BsaHI BsaI BsaJI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstNI BstUI BstXI
BstZ17I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoRII
EcoT14I EcoT22I FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KspI MaeI
MfeI MluI MluNI MscI MspA1I
MunI MvaI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 250 GGTACC G^GTACC
AccIII 96 TCCGGA T^CCGGA
AocI 115 CCTCAGG CC^T[ACGT]AGG
AseI 799 ATTAAT AT^TAAT
AsnI 799 ATTAAT AT^TAAT
Asp718I 250 GGTACC G^GTACC
AvaII 311 GGTCC G^G[AT]CC
BbsI 338 GAAGAC GAAGAC(2/6)
BcgI 276 CGATCCTCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 192 TGATCA T^GATCA
BcnI 859 CCCGG CC^[GC]GG
BglII 580 AGATCT A^GATCT
BpmI 473 CTCCAG CTGGAG(16/14)
BpuAI 338 GAAGAC GAAGAC(2/6)
BsaBI 382 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 96 TCCGGA T^CCGGA
BsmAI 431 GAGAC GTCTC(1/5)
BspEI 96 TCCGGA T^CCGGA
BspHI 483 TCATGA T^CATGA
BssKI 859 CCCGG ^CC[ACGT]GG
BstEII 922 GGTGACC G^GT[ACGT]ACC
BstPI 922 GGTGACC G^GT[ACGT]ACC
Bsu36I 115 CCTCAGG CC^T[ACGT]AGG
EarI 505 GAAGAG CTCTTC(1/4)
Eco81I 115 CCTCAGG CC^T[ACGT]AGG
EcoO65I 922 GGTGACC G^GT[ACGT]ACC
EcoRI 438 GAATTC G^AATTC
EcoRV 422 GATATC GAT^ATC
FauI 455 CCCGC CCCGC(4/6)
FbaI 192 TGATCA T^GATCA
HaeIII 449 GGCC GG^CC
HindIII 149 AAGCTT A^AGCTT
HinfI 361 GATTC G^A[ACGT]TC
KpnI 250 GGTACC GGTAC^C
Ksp632I 505 GAAGAG CTCTTC(1/4)
MaeII 918 ACGT A^CGT
MamI 382 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 96 TCCGGA T^CCGGA
MslI 828 CATACCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 286 GCTACATTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 859 CCCGG CC^[GC]GG
PshBI 799 ATTAAT AT^TAAT
RcaI 483 TCATGA T^CATGA
ScaI 984 AGTACT AGT^ACT
ScrFI 859 CCCGG CC^[ACGT]GG
TaqI 546 TCGA T^CGA
TfiI 361 GATTC G^A[AT]TC
Tsp45I 923 GTGAC ^GT[GC]AC
TspRI 812 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 546 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AciI 456 CCGC CCGC(-3/-1)
621 GCGG
AluI 42 AGCT AG^CT
150 AGCT
BanI 250 GGTACC G^G[CT][GA]CC
631 GGCACC
BsaWI 96 TCCGGA [AT]^CCGG[AT]
257 TCCGGT
BsiYI 649 CCAAGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
860 CCGGAATGAGG
BslI 649 CCAAGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
860 CCGGAATGAGG
BsrI 813 ACTGG ACTGG(1/-1)
475 CCAGT
BstYI 468 AGATCC [GA]^GATC[CT]
580 AGATCT
Cfr13I 311 GGTCC G^G[ACGT]CC
448 GGGCC
DraI 133 TTTAAA TTT^AAA
727 TTTAAA
HphI 385 GGTGA GGTGA(8/7)
922 GGTGA
MaeIII 575 GTTAC ^GT[ACGT]AC
923 GTGAC
MflI 468 AGATCC [GA]^GATC[CT]
580 AGATCT
Sau96I 311 GGTCC G^G[ACGT]CC
448 GGGCC
XhoII 468 AGATCC [GA]^GATC[CT]
580 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AcsI AfaI AlwI ApoI BbvI
BseRI BstF5I DdeI DpnII Fnu4HI
FokI HapII HpaII ItaI MboI
MboII MnlI MseI MspI NdeII
NlaIII NlaIV RsaI Sau3AI Tru9I
TseI Tsp509I