Restriction map for
pFN21AB5677
(Circular) (Six-base) MAPSORT of: pFN21AB5677.seq Check: 9893 from: 1 to: 5716
pFN21AB5677 5716 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3526 278
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3059 1937
Size: 1122 4594
Acc65I G'GTAC_C
Cuts at: 2255 3037 2255
Size: 782 4934
AclI AA'CG_TT
Cuts at: 1563 3843 4216 1563
Size: 2280 373 3063
Fragments arranged by size:
3063 2280 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2034 2391 3779 5163 5431 1632
Size: 402 357 1388 1384 268 1917
Fragments arranged by size:
1917 1388 1384 402 357 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5519
AflIII A'CryG_T
Cuts at: 1203 4615 5592 1203
Size: 3412 977 1327
Fragments arranged by size:
3412 1327 977
AgeI A'CCGG_T
Cuts at: 4547 4547
Size: 5716
AhdI GACnn_n'nnGTC
Cuts at: 4445 4445
Size: 5716
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5716
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5716
AlwNI CAG_nnn'CTG
Cuts at: 3459 5031 3459
Size: 1572 4144
ApaLI G'TGCA_C
Cuts at: 3773 4929 3773
Size: 1156 4560
ApoI r'AATT_y
Cuts at: 1493 2093 2135 2331 2443 2598 2729 2942 3025
Size: 600 42 196 112 155 131 213 83
Cuts at: 3025 3251 3403 5512 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 600 226 213 196 155 152
131 112 83 42
AseI AT'TA_AT
Cuts at: 160 1032 2805 4270 160
Size: 872 1773 1465 1606
Fragments arranged by size:
1773 1606 1465 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
AvaI C'yCGr_G
Cuts at: 1870 1943 3041 1870
Size: 73 1098 4545
Fragments arranged by size:
4545 1098 73
BamHI G'GATC_C
Cuts at: 3046 3413 3046
Size: 367 5349
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2255 2636 3037 4492 618
Size: 325 217 627 468 381 401 1455 1842
Fragments arranged by size:
1842 1455 627 468 401 381 325 217
BanII G_rGCy'C
Cuts at: 1391 3035 1391
Size: 1644 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2350 928
Size: 971 451 4294
Fragments arranged by size:
4294 971 451
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2270 2304 3907 3941 1903
Size: 34 333 34 1603 34 3678
Fragments arranged by size:
3678 1603 333 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3608 4818 3608
Size: 1210 4506
BclI T'GATC_A
Cuts at: 1548 2197 1548
Size: 649 5067
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4327 136
Size: 107 122 71 631 605 2655 1525
Fragments arranged by size:
2655 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2585 5711 2585
Size: 3126 2590
BlpI GC'TnA_GC
Cuts at: 3133 3133
Size: 5716
Bme1580I G_kGCm'C
Cuts at: 1790 3777 4933 1790
Size: 1987 1156 2573
Fragments arranged by size:
2573 1987 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5716
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3447 4405 442
Size: 416 53 83 399 24 2030 958 1753
Fragments arranged by size:
2030 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2463 4376 1239
Size: 1224 1913 2579
Fragments arranged by size:
2579 1913 1224
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3841 4706 5004 3841
Size: 865 298 4553
Fragments arranged by size:
4553 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4379 882
Size: 617 2880 2219
Fragments arranged by size:
2880 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3507 493
Size: 711 2303 2702
Fragments arranged by size:
2702 2303 711
BsaBI GATnn'nnATC
Cuts at: 2391 5710 2391
Size: 3319 2397
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3523 3905 4540 275
Size: 53 83 186 936 1990 382 635 1451
Fragments arranged by size:
1990 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2101 2262 4149 4547 4821 4968 5379 2101
Size: 161 1887 398 274 147 411 2438
Fragments arranged by size:
2438 1887 411 398 274 161 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2201 2231 1178
Size: 30 165 30 798 30 4663
Fragments arranged by size:
4663 798 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2109 2241 2887 1186
Size: 923 132 646 4015
Fragments arranged by size:
4015 923 646 132
BseYI C'CCAG_C
Cuts at: 4919 5415 4919
Size: 496 5220
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5143
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3166 3927 4955 1540
Size: 275 186 1165 761 1028 2301
Fragments arranged by size:
2301 1165 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3777 3862 4933 3777
Size: 85 1071 4560
Fragments arranged by size:
4560 1071 85
BsmI GAATG_Cn'
Cuts at: 3227 3320 3227
Size: 93 5623
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3035 3777 3862 4933 1391
Size: 399 1245 742 85 1071 2174
Fragments arranged by size:
2174 1245 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2101 5379 2101
Size: 3278 2438
BspHI T'CATG_A
Cuts at: 2488 3605 5335 2488
Size: 1117 1730 2869
Fragments arranged by size:
2869 1730 1117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3071 844
Size: 2227 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3603 1394
Size: 429 1780 3507
Fragments arranged by size:
3507 1780 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4211 4385 65
Size: 1183 2963 174 1396
Fragments arranged by size:
2963 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4360 4547 1700
Size: 110 2550 187 2869
Fragments arranged by size:
2869 2550 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5716
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5716
BssSI C'ACGA_G
Cuts at: 3776 4788 3776
Size: 1012 4704
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5716
BstBI TT'CG_AA
Cuts at: 4520 4520
Size: 5716
BstEII G'GTnAC_C
Cuts at: 1184 2927 1184
Size: 1743 3973
BstYI r'GATC_y
Cuts at: 1972 2473 2585 3046 3413 3798 3815 5267 5353
Size: 501 112 461 367 385 17 1452 86
Cuts at: 5353 5365 5711 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 501 461 385 367 346 112
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2121 2121
Size: 5716
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4225
Fragments arranged by size:
4225 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4977
Fragments arranged by size:
4977 683 56
BtsI GCAGTG_nn'
Cuts at: 3232 4026 4046 3232
Size: 794 20 4902
Fragments arranged by size:
4902 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5716
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5681
DraI TTT'AAA
Cuts at: 2140 2734 3020 2140
Size: 594 286 4836
Fragments arranged by size:
4836 594 286
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4723 809
Size: 507 3407 1802
Fragments arranged by size:
3407 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3163 4052 5681
Size: 54 1425 50 275 18 1333 889 1629
Cuts at: 5681 8
Size: 43
Fragments arranged by size:
1629 1425 1333 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3163 1537
Size: 275 1351 4090
Fragments arranged by size:
4090 1351 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2505 3646 1353
Size: 1152 1141 3423
Fragments arranged by size:
3423 1152 1141
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2641 4303 4677 4823 387
Size: 1064 138 1052 1662 374 146 1280
Fragments arranged by size:
1662 1280 1064 1052 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 2391 2463 3779 4376 5163 5431
Size: 393 402 357 72 1316 597 787 268
Cuts at: 5431 1239
Size: 1524
Fragments arranged by size:
1524 1316 787 597 402 393 357 268
72
EcoRI G'AATT_C
Cuts at: 2443 3025 3403 2443
Size: 582 378 4756
Fragments arranged by size:
4756 582 378
EcoRV GAT'ATC
Cuts at: 2429 2429
Size: 5716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3008 3008
Size: 5716
FspI TGC'GCA
Cuts at: 4222 4222
Size: 5716
HaeII r_GCGC'y
Cuts at: 1678 4863 1678
Size: 3185 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2954 2986 4357 4389 4431
Size: 32 135 32 1382 32 1371 32 42
Cuts at: 4431 4463 1373
Size: 32 2626
Fragments arranged by size:
2626 1382 1371 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3060 3306 669
Size: 988 281 1122 246 3079
Fragments arranged by size:
3079 1122 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2154 748
Size: 1406 4310
HpaI GTT'AAC
Cuts at: 3306 3306
Size: 5716
KpnI G_GTAC'C
Cuts at: 2259 3041 2259
Size: 782 4934
MfeI C'AATT_G
Cuts at: 3315 3315
Size: 5716
MluI A'CGCG_T
Cuts at: 5592 5592
Size: 5716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2343 4830 5014 1305
Size: 1038 2487 184 2007
Fragments arranged by size:
2487 2007 1038 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3894
Fragments arranged by size:
3894 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2837 3674 4033 4192 518
Size: 648 1671 837 359 159 2042
Fragments arranged by size:
2042 1671 837 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3132 3809 4957 5202 1606
Size: 1526 677 1148 245 2120
Fragments arranged by size:
2120 1526 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4225
Fragments arranged by size:
4225 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5716
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5716
NotI GC'GGCC_GC
Cuts at: 3163 3163
Size: 5716
NspI r_CATG'y
Cuts at: 1132 1229 3074 4619 1132
Size: 97 1845 1545 2229
Fragments arranged by size:
2229 1845 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5716
PciI A'CATG_T
Cuts at: 4615 4615
Size: 5716
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5716
PmeI GTTT'AAAC
Cuts at: 3020 3020
Size: 5716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3785 3817 5298 5330 3785
Size: 32 1481 32 4171
Fragments arranged by size:
4171 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5716
PsiI TTA'TAA
Cuts at: 3286 3286
Size: 5716
PstI C_TGCA'G
Cuts at: 830 3068 830
Size: 2238 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5716
SalI G'TCGA_C
Cuts at: 1936 3058 1936
Size: 1122 4594
SbfI CC_TGCA'GG
Cuts at: 3068 3068
Size: 5716
ScaI AGT'ACT
Cuts at: 2991 3964 5676 2991
Size: 973 1712 3031
Fragments arranged by size:
3031 1712 973
SfcI C'TryA_G
Cuts at: 826 1045 3064 4199 4880 5071 826
Size: 219 2019 1135 681 191 1471
Fragments arranged by size:
2019 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5716
SmaI CCC'GGG
Cuts at: 1872 3043 1872
Size: 1171 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3820 4721 4983 820
Size: 197 926 1877 901 262 1553
Fragments arranged by size:
1877 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3507 493
Size: 3014 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5716
SphI G_CATG'C
Cuts at: 1132 3074 1132
Size: 1942 3774
SspI AAT'ATT
Cuts at: 5 52 3640 5
Size: 47 3588 2081
Fragments arranged by size:
3588 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5716
StyI C'CwwG_G
Cuts at: 513 1065 2004 3155 513
Size: 552 939 1151 3074
Fragments arranged by size:
3074 1151 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2342 3743 3760 3913 4098 4543 325
Size: 1255 762 1401 17 153 185 445 1498
Fragments arranged by size:
1498 1401 1255 762 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2989 3962 5674
Size: 274 80 33 51 1443 1012 973 1712
Cuts at: 5674 96
Size: 138
Fragments arranged by size:
1712 1443 1012 973 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5676 5708 5676
Size: 32 5684
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5716
XbaI T'CTAG_A
Cuts at: 3052 3052
Size: 5716
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5716
XmaI C'CCGG_G
Cuts at: 1870 3041 1870
Size: 1171 4545
XmnI GAAnn'nnTTC
Cuts at: 3845 3845
Size: 5716
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3524 276
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI