Restriction map for
pFN21AB5647
(Circular) (Six-base) MAPSORT of: pFN21AB5647.seq Check: 359 from: 1 to: 5656
pFN21AB5647 5656 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3466 278
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2999 1937
Size: 1062 4594
Acc65I G'GTAC_C
Cuts at: 2977 2977
Size: 5656
AclI AA'CG_TT
Cuts at: 1563 3783 4156 1563
Size: 2220 373 3063
Fragments arranged by size:
3063 2220 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2446 2723 3719 5103 5371 1632
Size: 814 277 996 1384 268 1917
Fragments arranged by size:
1917 1384 996 814 277 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5459
AflIII A'CryG_T
Cuts at: 1203 4555 5532 1203
Size: 3352 977 1327
Fragments arranged by size:
3352 1327 977
AgeI A'CCGG_T
Cuts at: 4487 4487
Size: 5656
AhdI GACnn_n'nnGTC
Cuts at: 2603 4385 2603
Size: 1782 3874
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5656
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5656
AlwNI CAG_nnn'CTG
Cuts at: 3399 4971 3399
Size: 1572 4084
ApaI G_GGCC'C
Cuts at: 2594 2594
Size: 5656
ApaLI G'TGCA_C
Cuts at: 3713 4869 3713
Size: 1156 4500
ApoI r'AATT_y
Cuts at: 1493 2342 2728 2834 2965 3191 3343 5452 1493
Size: 849 386 106 131 226 152 2109 1697
Fragments arranged by size:
2109 1697 849 386 226 152 131 106
AseI AT'TA_AT
Cuts at: 160 1032 2919 4210 160
Size: 872 1887 1291 1606
Fragments arranged by size:
1887 1606 1291 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
AvaI C'yCGr_G
Cuts at: 1870 1943 2178 2981 1870
Size: 73 235 803 4545
Fragments arranged by size:
4545 803 235 73
BamHI G'GATC_C
Cuts at: 2086 2986 3353 2086
Size: 900 367 4389
Fragments arranged by size:
4389 900 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2066 2977 4432 618
Size: 325 217 627 279 911 1455 1842
Fragments arranged by size:
1842 1455 911 627 325 279 217
BanII G_rGCy'C
Cuts at: 1391 2594 2895 2975 1391
Size: 1203 301 80 4072
Fragments arranged by size:
4072 1203 301 80
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4685
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3847 3881 1903
Size: 34 1910 34 3678
Fragments arranged by size:
3678 1910 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3548 4758 3548
Size: 1210 4446
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5656
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2189 4267 136
Size: 107 122 71 631 605 517 2078 1525
Fragments arranged by size:
2078 1525 631 605 517 122 107 71
BglII A'GATC_T
Cuts at: 5651 5651
Size: 5656
BlpI GC'TnA_GC
Cuts at: 2552 2894 3073 2552
Size: 342 179 5135
Fragments arranged by size:
5135 342 179
Bme1580I G_kGCm'C
Cuts at: 1790 2594 3717 4873 1790
Size: 804 1123 1156 2573
Fragments arranged by size:
2573 1156 1123 804
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5656
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3387 4345 442
Size: 416 53 83 399 24 1970 958 1753
Fragments arranged by size:
1970 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2888 4316 1239
Size: 1649 1428 2579
Fragments arranged by size:
2579 1649 1428
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3781 4646 4944 3781
Size: 865 298 4493
Fragments arranged by size:
4493 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4319 882
Size: 617 2820 2219
Fragments arranged by size:
2820 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3447 493
Size: 711 2243 2702
Fragments arranged by size:
2702 2243 711
BsaBI GATnn'nnATC
Cuts at: 5650 5650
Size: 5656
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3463 3845 4480 275
Size: 53 83 186 936 1930 382 635 1451
Fragments arranged by size:
1930 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4089 4487 4761 4908 5319 4089
Size: 398 274 147 411 4426
Fragments arranged by size:
4426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5431
Fragments arranged by size:
5431 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5656
BseYI C'CCAG_C
Cuts at: 2547 2952 4859 5355 2547
Size: 405 1907 496 2848
Fragments arranged by size:
2848 1907 496 405
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5083
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3106 3867 4895 1540
Size: 275 186 1105 761 1028 2301
Fragments arranged by size:
2301 1105 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2895 3717 3802 4873 2895
Size: 822 85 1071 3678
Fragments arranged by size:
3678 1071 822 85
BsmI GAATG_Cn'
Cuts at: 2632 3167 3260 2632
Size: 535 93 5028
Fragments arranged by size:
5028 535 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2594 2895 2975 3717 3802 4873 1391
Size: 399 804 301 80 742 85 1071 2174
Fragments arranged by size:
2174 1071 804 742 399 301 85 80
BspEI T'CCGG_A
Cuts at: 5319 5319
Size: 5656
BspHI T'CATG_A
Cuts at: 3545 5275 3545
Size: 1730 3926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3011 844
Size: 2167 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3543 1394
Size: 429 1720 3507
Fragments arranged by size:
3507 1720 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4151 4325 65
Size: 1183 2903 174 1396
Fragments arranged by size:
2903 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2222 4300 4487 1700
Size: 110 412 2078 187 2869
Fragments arranged by size:
2869 2078 412 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5656
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5656
BssSI C'ACGA_G
Cuts at: 3716 4728 3716
Size: 1012 4644
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2235 794
Size: 1441 4215
BstBI TT'CG_AA
Cuts at: 4460 4460
Size: 5656
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5656
BstYI r'GATC_y
Cuts at: 1972 2086 2149 2986 3353 3738 3755 5207 5293
Size: 114 63 837 367 385 17 1452 86
Cuts at: 5293 5305 5651 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 837 385 367 346 114 86
63 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2586 513
Size: 552 1521 3583
Fragments arranged by size:
3583 1521 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4917
Fragments arranged by size:
4917 683 56
BtsI GCAGTG_nn'
Cuts at: 2173 3172 3966 3986 2173
Size: 999 794 20 3843
Fragments arranged by size:
3843 999 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5656
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5621
DraI TTT'AAA
Cuts at: 2887 2960 2887
Size: 73 5583
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4663 809
Size: 507 3347 1802
Fragments arranged by size:
3347 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2383 3103 3992
Size: 54 1425 50 275 18 553 720 889
Cuts at: 3992 5621 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 720 553 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3103 1537
Size: 275 1291 4090
Fragments arranged by size:
4090 1291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2265 2754 3586 1353
Size: 912 489 832 3423
Fragments arranged by size:
3423 912 832 489
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4243 4617 4763 387
Size: 1064 138 2654 374 146 1280
Fragments arranged by size:
2654 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2893 2893
Size: 5656
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2446 2723 2888 3719 4316 5103 5371
Size: 393 814 277 165 831 597 787 268
Cuts at: 5371 1239
Size: 1524
Fragments arranged by size:
1524 831 814 787 597 393 277 268
165
EcoO109I rG'GnC_Cy
Cuts at: 2591 2591
Size: 5656
EcoRI G'AATT_C
Cuts at: 2834 2965 3343 2834
Size: 131 378 5147
Fragments arranged by size:
5147 378 131
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2748 2748
Size: 5656
FspI TGC'GCA
Cuts at: 4162 4162
Size: 5656
HaeII r_GCGC'y
Cuts at: 1678 4803 1678
Size: 3125 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2589 2621 4297 4329 4371
Size: 32 135 32 1017 32 1676 32 42
Cuts at: 4371 4403 1373
Size: 32 2626
Fragments arranged by size:
2626 1676 1017 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2653 3000 3246 669
Size: 988 281 715 347 246 3079
Fragments arranged by size:
3079 988 715 347 281 246
HindIII A'AGCT_T
Cuts at: 748 2641 748
Size: 1893 3763
HpaI GTT'AAC
Cuts at: 3246 3246
Size: 5656
KpnI G_GTAC'C
Cuts at: 2981 2981
Size: 5656
MfeI C'AATT_G
Cuts at: 2448 3255 2448
Size: 807 4849
MluI A'CGCG_T
Cuts at: 5532 5532
Size: 5656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4770 4954 1305
Size: 3465 184 2007
Fragments arranged by size:
3465 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2385 10
Size: 54 1425 343 553 3281
Fragments arranged by size:
3281 1425 553 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2585 3614 3973 4132 518
Size: 648 1419 1029 359 159 2042
Fragments arranged by size:
2042 1419 1029 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2083 3072 3749 4897 5142 1606
Size: 477 989 677 1148 245 2120
Fragments arranged by size:
2120 1148 989 677 477 245
NaeI GCC'GGC
Cuts at: 2224 2224
Size: 5656
NcoI C'CATG_G
Cuts at: 513 1065 2586 513
Size: 552 1521 3583
Fragments arranged by size:
3583 1521 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5656
NgoMIV G'CCGG_C
Cuts at: 2222 2222
Size: 5656
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5656
NotI GC'GGCC_GC
Cuts at: 3103 3103
Size: 5656
NspI r_CATG'y
Cuts at: 1132 1229 2120 3014 4559 1132
Size: 97 891 894 1545 2229
Fragments arranged by size:
2229 1545 894 891 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5656
PciI A'CATG_T
Cuts at: 4555 4555
Size: 5656
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2537 2938 1108
Size: 1429 401 3826
Fragments arranged by size:
3826 1429 401
PfoI T'CCnGG_A
Cuts at: 2089 2089
Size: 5656
PmeI GTTT'AAAC
Cuts at: 2960 2960
Size: 5656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2511 2543 3725 3757 5238 5270 2511
Size: 32 1182 32 1481 32 2897
Fragments arranged by size:
2897 1481 1182 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5656
PsiI TTA'TAA
Cuts at: 3226 3226
Size: 5656
PspOMI G'GGCC_C
Cuts at: 2590 2590
Size: 5656
PspXI vC'TCGA_Gb
Cuts at: 2178 2178
Size: 5656
PstI C_TGCA'G
Cuts at: 830 2157 3008 830
Size: 1327 851 3478
Fragments arranged by size:
3478 1327 851
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5656
PvuII CAG'CTG
Cuts at: 2083 2083
Size: 5656
SacI G_AGCT'C
Cuts at: 2895 2895
Size: 5656
SalI G'TCGA_C
Cuts at: 1936 2998 1936
Size: 1062 4594
SbfI CC_TGCA'GG
Cuts at: 3008 3008
Size: 5656
ScaI AGT'ACT
Cuts at: 3904 5616 3904
Size: 1712 3944
SfcI C'TryA_G
Cuts at: 826 1045 2027 2153 3004 4139 4820 5011 826
Size: 219 982 126 851 1135 681 191 1471
Fragments arranged by size:
1471 1135 982 851 681 219 191 126
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5656
SmaI CCC'GGG
Cuts at: 1872 2983 1872
Size: 1111 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2178 3760 4661 4923 820
Size: 197 926 235 1582 901 262 1553
Fragments arranged by size:
1582 1553 926 901 262 235 197
SnaBI TAC'GTA
Cuts at: 493 3447 493
Size: 2954 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5656
SphI G_CATG'C
Cuts at: 1132 3014 1132
Size: 1882 3774
SspI AAT'ATT
Cuts at: 5 52 2440 2710 2770 3580 5
Size: 47 2388 270 60 810 2081
Fragments arranged by size:
2388 2081 810 270 60 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5656
StyI C'CwwG_G
Cuts at: 513 1065 2586 3095 513
Size: 552 1521 509 3074
Fragments arranged by size:
3074 1521 552 509
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3683 3700 3853 4038 4483 325
Size: 1255 2103 17 153 185 445 1498
Fragments arranged by size:
2103 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2799 2871 3902
Size: 274 80 33 51 1443 822 72 1031
Cuts at: 3902 5614 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1031 822 274 138 80 72
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5616 5648 5616
Size: 32 5624
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5656
XbaI T'CTAG_A
Cuts at: 2992 2992
Size: 5656
XhoI C'TCGA_G
Cuts at: 1943 2178 1943
Size: 235 5421
XmaI C'CCGG_G
Cuts at: 1870 2981 1870
Size: 1111 4545
XmnI GAAnn'nnTTC
Cuts at: 2526 3785 2526
Size: 1259 4397
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3464 276
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PmlI PpuMI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI