Restriction map for
pFN21AB5636
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BbsI BcgI BciVI BclI
BfrI BglII BlnI BpuAI BsaAI
BsaBI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI DsaI Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluI MroI MslI MunI
NcoI NdeI NheI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsaI RsrII SacII SalI SapI
SbfI ScaI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tsp509I
Tth111I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AlwNI 424 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 292 GGGCCC GGGCC^C
AscI 820 GGCGCGCC GG^CGCGCC
AspHI 625 GAGCTC G[AT]GC[AT]^C
BbeI 205 GGCGCC GGCGC^C
BbvCI 417 CCTCAGC CCTCAGC(-5/-2)
BfaI 46 CTAG C^TAG
BlpI 666 GCTCAGC GC^T[ACGT]AGC
BpmI 864 CTCCAG CTGGAG(16/14)
Bpu1102I 666 GCTCAGC GC^T[ACGT]AGC
BsaI 507 GAGACC GGTCTC(1/5)
BsiHKAI 625 GAGCTC G[AT]GC[AT]^C
BsmBI 118 GAGACG CGTCTC(1/5)
BsrBI 7 GAGCGG CCGCTC(-3/-3)
BstAPI 843 GCAAGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 680 AGATCC [GA]^GATC[CT]
CelII 666 GCTCAGC GC^T[ACGT]AGC
EarI 501 GAAGAG CTCTTC(1/4)
EcoT14I 113 CCAAGG C^C[AT][AT]GG
HaeII 205 GGCGCC [GA]GCGC^[CT]
HinfI 363 GAGTC G^A[ACGT]TC
KasI 205 GGCGCC G^GCGCC
Ksp632I 501 GAAGAG CTCTTC(1/4)
MaeI 46 CTAG C^TAG
MaeII 848 ACGT A^CGT
MflI 680 AGATCC [GA]^GATC[CT]
MseI 220 TTAA T^TAA
NaeI 982 GCCGGC GCC^GGC
NarI 205 GGCGCC GG^CGCC
NgoMIV 982 GCCGGC G^CCGGC
PflMI 423 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 363 GAGTC GAGTC(4/5)
PpuMI 773 AGGACCC [GA]G^G[AT]CC[CT]
SacI 625 GAGCTC GAGCT^C
SexAI 89 ACCTGGT A^CC[AT]GGT
SfaNI 466 GATGC GCATC(5/9)
SfcI 411 CTACAG C^T[GA][CT]AG
SfiI 69 GGCCAGCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 205 GGCGCC GGC^GCC
StyI 113 CCAAGG C^C[AT][AT]GG
Tru9I 220 TTAA T^TAA
Tsp45I 35 GTGAC ^GT[GC]AC
TspRI 33 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 423 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 680 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 106 GGCGTC G[GA]^CG[CT]C
205 GGCGCC
AlwI 639 GATCC GGATC(4/5)
681 GATCC
AvaI 177 CCCGGG C^[CT]CG[GA]G
289 CCCGGG
BalI 692 TGGCCA TGG^CCA
716 TGGCCA
BglI 70 GCCAGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
176 GCCCGGGGGGC
BsaHI 106 GGCGTC G[GA]^CG[CT]C
205 GGCGCC
BsgI 279 GTGCAG GTGCAG(16/14)
355 GTGCAG
BsoBI 177 CCCGGG C^[CT]CG[GA]G
289 CCCGGG
BsrFI 193 GCCGGT [GA]^CCGG[CT]
982 GCCGGC
Cfr10I 193 GCCGGT [GA]^CCGG[CT]
982 GCCGGC
DpnII 639 GATC ^GATC
681 GATC
DraII 613 GGGGCCT [GA]G^G[ACGT]CC[CT]
773 AGGACCC
EcoO109I 613 GGGGCCT [GA]G^G[ACGT]CC[CT]
773 AGGACCC
Hin1I 106 GGCGTC G[GA]^CG[CT]C
205 GGCGCC
HphI 482 TCACC GGTGA(8/7)
572 TCACC
MaeIII 35 GTGAC ^GT[ACGT]AC
322 GTTAC
MboI 639 GATC ^GATC
681 GATC
MboII 501 GAAGA GAAGA(8/7)
569 TCTTC
MluNI 692 TGGCCA TGG^CCA
716 TGGCCA
MscI 692 TGGCCA TGG^CCA
716 TGGCCA
NdeII 639 GATC ^GATC
681 GATC
NotI 9 GCGGCCGC GC^GGCCGC
130 GCGGCCGC
PvuII 358 CAGCTG CAG^CTG
867 CAGCTG
Sau3AI 639 GATC ^GATC
681 GATC
SmaI 177 CCCGGG CCC^GGG
289 CCCGGG
TaqI 155 TCGA T^CGA
495 TCGA
TthHB8I 155 TCGA T^CGA
495 TCGA
XmaCI 177 CCCGGG C^CCGGG
289 CCCGGG
XmaI 177 CCCGGG C^CCGGG
289 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AluI AvaII BanI
BanII BbvI BcnI BmyI BsaJI
BseRI BsiEI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssHII BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI EaeI EagI EclXI
Eco52I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HpaII ItaI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV Sau96I ScrFI TseI