(Circular) (Six-base) MAPSORT of: pFN21AB5597.seq Check: 1644 from: 1 to: 6091 pFN21AB5597 6091 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2974 2974 Size: 6091 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3901 278 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3229 3434 1937 Size: 1292 205 4594 Fragments arranged by size: 4594 1292 205 Acc65I G'GTAC_C Cuts at: 3069 3412 3069 Size: 343 5748 AclI AA'CG_TT Cuts at: 1563 4218 4591 1563 Size: 2655 373 3063 Fragments arranged by size: 3063 2655 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2366 3212 4154 5538 5806 1632 Size: 734 846 942 1384 268 1917 Fragments arranged by size: 1917 1384 942 846 734 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5894 AflIII A'CryG_T Cuts at: 1203 4990 5967 1203 Size: 3787 977 1327 Fragments arranged by size: 3787 1327 977 AgeI A'CCGG_T Cuts at: 4922 4922 Size: 6091 AhdI GACnn_n'nnGTC Cuts at: 4820 4820 Size: 6091 AleI CACnn'nnGTG Cuts at: 1166 2315 1166 Size: 1149 4942 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2333 1184 Size: 1149 4942 AlwNI CAG_nnn'CTG Cuts at: 2026 2057 2342 2653 2704 2818 2971 3040 3834 Size: 31 285 311 51 114 153 69 794 Cuts at: 3834 5406 2026 Size: 1572 2711 Fragments arranged by size: 2711 1572 794 311 285 153 114 69 51 31 ApaLI G'TGCA_C Cuts at: 4148 5304 4148 Size: 1156 4935 ApoI r'AATT_y Cuts at: 1493 2486 3400 3626 3778 5887 1493 Size: 993 914 226 152 2109 1697 Fragments arranged by size: 2109 1697 993 914 226 152 AseI AT'TA_AT Cuts at: 160 1032 4645 160 Size: 872 3613 1606 Fragments arranged by size: 3613 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 AvaI C'yCGr_G Cuts at: 1870 1943 2217 3416 1870 Size: 73 274 1199 4545 Fragments arranged by size: 4545 1199 274 73 BamHI G'GATC_C Cuts at: 3421 3788 3421 Size: 367 5724 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2123 2934 3069 3279 3412 Size: 325 217 627 336 811 135 210 133 Cuts at: 3412 4867 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 811 627 336 325 217 210 135 133 BanII G_rGCy'C Cuts at: 1391 3017 3410 1391 Size: 1626 393 4072 Fragments arranged by size: 4072 1626 393 BbeI G_GCGC'C Cuts at: 2938 3283 2938 Size: 345 5746 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3319 928 Size: 971 1420 3700 Fragments arranged by size: 3700 1420 971 BbvCI CC'TCA_GC Cuts at: 2640 3284 3366 2640 Size: 644 82 5365 Fragments arranged by size: 5365 644 82 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2737 2771 4282 4316 1903 Size: 34 800 34 1511 34 3678 Fragments arranged by size: 3678 1511 800 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2305 3983 5193 2305 Size: 1678 1210 3203 Fragments arranged by size: 3203 1678 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6091 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2573 2759 4702 Size: 107 122 71 631 605 901 186 1943 Cuts at: 4702 136 Size: 1525 Fragments arranged by size: 1943 1525 901 631 605 186 122 107 71 BglII A'GATC_T Cuts at: 6086 6086 Size: 6091 BlpI GC'TnA_GC Cuts at: 2654 3508 2654 Size: 854 5237 Bme1580I G_kGCm'C Cuts at: 1790 2126 4152 5308 1790 Size: 336 2026 1156 2573 Fragments arranged by size: 2573 2026 1156 336 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6091 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3822 4780 442 Size: 416 53 83 399 24 2405 958 1753 Fragments arranged by size: 2405 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6091 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2134 2260 2816 3164 4751 1239 Size: 895 126 556 348 1587 2579 Fragments arranged by size: 2579 1587 895 556 348 126 Bpu10I CC'TnA_GC Cuts at: 2640 3284 3366 2640 Size: 644 82 5365 Fragments arranged by size: 5365 644 82 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2867 4216 5081 5379 2867 Size: 1349 865 298 3579 Fragments arranged by size: 3579 1349 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2684 4754 882 Size: 617 1185 2070 2219 Fragments arranged by size: 2219 2070 1185 617 BsaAI yAC'GTr Cuts at: 493 1204 3882 493 Size: 711 2678 2702 Fragments arranged by size: 2702 2678 711 BsaBI GATnn'nnATC Cuts at: 6085 6085 Size: 6091 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2935 3280 3898 4280 Size: 53 83 186 936 1402 345 618 382 Cuts at: 4280 4915 275 Size: 635 1451 Fragments arranged by size: 1451 1402 936 635 618 382 345 186 83 53 BsaWI w'CCGG_w Cuts at: 3019 4524 4922 5196 5343 5754 3019 Size: 1505 398 274 147 411 3356 Fragments arranged by size: 3356 1505 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2450 2480 2513 2543 1178 Size: 30 165 30 1047 30 33 30 4726 Fragments arranged by size: 4726 1047 165 33 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2093 2098 2137 2263 2657 1186 Size: 907 5 39 126 394 4620 Fragments arranged by size: 4620 907 394 126 39 5 BseYI C'CCAG_C Cuts at: 2306 2324 2329 2335 5294 5790 2306 Size: 18 5 6 2959 496 2607 Fragments arranged by size: 2959 2607 496 18 6 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2573 2693 2831 1163 Size: 573 837 120 138 4423 Fragments arranged by size: 4423 837 573 138 120 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3257 3541 4302 5330 1540 Size: 275 186 1256 284 761 1028 2301 Fragments arranged by size: 2301 1256 1028 761 284 275 186 BsiHKAI G_wGCw'C Cuts at: 3017 3264 4152 4237 5308 3017 Size: 247 888 85 1071 3800 Fragments arranged by size: 3800 1071 888 247 85 BsmI GAATG_Cn' Cuts at: 3602 3695 3602 Size: 93 5998 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2126 3017 3264 3410 4152 4237 5308 Size: 399 336 891 247 146 742 85 1071 Cuts at: 5308 1391 Size: 2174 Fragments arranged by size: 2174 1071 891 742 399 336 247 146 85 BspEI T'CCGG_A Cuts at: 3019 5754 3019 Size: 2735 3356 BspHI T'CATG_A Cuts at: 2775 3980 5710 2775 Size: 1205 1730 3156 Fragments arranged by size: 3156 1730 1205 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2712 2799 2974 3446 844 Size: 1868 87 175 472 3489 Fragments arranged by size: 3489 1868 472 175 87 BsrBI CCG'CTC Cuts at: 1394 1823 2067 3978 1394 Size: 429 244 1911 3507 Fragments arranged by size: 3507 1911 429 244 BsrDI GCAATG_nn' Cuts at: 65 1248 3213 3348 4586 4760 65 Size: 1183 1965 135 1238 174 1396 Fragments arranged by size: 1965 1396 1238 1183 174 135 BsrFI r'CCGG_y Cuts at: 1700 1810 2931 3256 4735 4922 1700 Size: 110 1121 325 1479 187 2869 Fragments arranged by size: 2869 1479 1121 325 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6091 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6091 BssSI C'ACGA_G Cuts at: 4151 5163 4151 Size: 1012 5079 BstAPI GCAn_nnn'nTGC Cuts at: 794 2818 794 Size: 2024 4067 BstBI TT'CG_AA Cuts at: 4895 4895 Size: 6091 BstEII G'GTnAC_C Cuts at: 1184 2386 1184 Size: 1202 4889 BstXI CCAn_nnnn'nTGG Cuts at: 2239 2398 2239 Size: 159 5932 BstYI r'GATC_y Cuts at: 1972 2395 3421 3788 4173 4190 5642 5728 5740 Size: 423 1026 367 385 17 1452 86 12 Cuts at: 5740 6086 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1026 423 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4600 Fragments arranged by size: 4600 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5352 Fragments arranged by size: 5352 683 56 BtsI GCAGTG_nn' Cuts at: 2055 3051 3607 4401 4421 2055 Size: 996 556 794 20 3725 Fragments arranged by size: 3725 996 794 556 20 ClaI AT'CG_AT Cuts at: 1554 3246 1554 Size: 1692 4399 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6056 DraI TTT'AAA Cuts at: 3395 3395 Size: 6091 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5098 809 Size: 507 3782 1802 Fragments arranged by size: 3782 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3254 3538 4427 Size: 54 1425 50 275 18 1424 284 889 Cuts at: 4427 6056 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1424 889 284 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3254 3538 1537 Size: 275 1442 284 4090 Fragments arranged by size: 4090 1442 284 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2360 4021 1353 Size: 1007 1661 3423 Fragments arranged by size: 3423 1661 1007 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4678 5052 5198 387 Size: 1064 138 3089 374 146 1280 Fragments arranged by size: 3089 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3015 3015 Size: 6091 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2134 2260 2366 2816 3164 3212 4154 Size: 393 502 126 106 450 348 48 942 Cuts at: 4154 4751 5538 5806 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 942 787 597 502 450 393 348 268 126 106 48 EcoNI CCTnn'n_nnAGG Cuts at: 2510 2795 2510 Size: 285 5806 EcoO109I rG'GnC_Cy Cuts at: 2623 2701 2752 2860 2902 3343 2623 Size: 78 51 108 42 441 5371 Fragments arranged by size: 5371 441 108 78 51 42 EcoRI G'AATT_C Cuts at: 3400 3778 3400 Size: 378 5713 EcoRV GAT'ATC Cuts at: 3165 3165 Size: 6091 FspI TGC'GCA Cuts at: 4597 4597 Size: 6091 HaeII r_GCGC'y Cuts at: 1678 2254 2938 3283 5238 1678 Size: 576 684 345 1955 2531 Fragments arranged by size: 2531 1955 684 576 345 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2066 2098 2380 2412 2450 Size: 32 135 32 494 32 282 32 38 Cuts at: 2450 2482 2886 2918 3222 3254 4732 4764 4806 Size: 32 404 32 304 32 1478 32 42 Cuts at: 4806 4838 1373 Size: 32 2626 Fragments arranged by size: 2626 1478 494 404 304 282 135 42 38 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3230 3435 3681 669 Size: 988 281 1292 205 246 3079 Fragments arranged by size: 3079 1292 988 281 246 205 HindIII A'AGCT_T Cuts at: 748 748 Size: 6091 HpaI GTT'AAC Cuts at: 3681 3681 Size: 6091 KasI G'GCGC_C Cuts at: 2934 3279 2934 Size: 345 5746 KpnI G_GTAC'C Cuts at: 3073 3416 3073 Size: 343 5748 MfeI C'AATT_G Cuts at: 3690 3690 Size: 6091 MluI A'CGCG_T Cuts at: 5967 5967 Size: 6091 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5205 5389 1305 Size: 3900 184 2007 Fragments arranged by size: 3900 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4269 Fragments arranged by size: 4269 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2315 2721 3008 4049 4408 4567 518 Size: 648 1149 406 287 1041 359 159 2042 Fragments arranged by size: 2042 1149 1041 648 406 359 287 159 MspA1I CmG'CkG Cuts at: 1606 2406 2493 2587 2650 2818 2959 3507 4184 Size: 800 87 94 63 168 141 548 677 Cuts at: 4184 5332 5577 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 800 677 548 245 168 141 94 87 63 NarI GG'CG_CC Cuts at: 2935 3280 2935 Size: 345 5746 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4600 Fragments arranged by size: 4600 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6091 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6091 NotI GC'GGCC_GC Cuts at: 3538 3538 Size: 6091 NspI r_CATG'y Cuts at: 1132 1229 2893 3449 4994 1132 Size: 97 1664 556 1545 2229 Fragments arranged by size: 2229 1664 1545 556 97 PasI CC'CwG_GG Cuts at: 1348 3097 1348 Size: 1749 4342 PciI A'CATG_T Cuts at: 4990 4990 Size: 6091 PflMI CCAn_nnn'nTGG Cuts at: 1108 2119 2342 3040 1108 Size: 1011 223 698 4159 Fragments arranged by size: 4159 1011 698 223 PfoI T'CCnGG_A Cuts at: 2517 2697 2904 3167 2517 Size: 180 207 263 5441 Fragments arranged by size: 5441 263 207 180 PmeI GTTT'AAAC Cuts at: 3395 3395 Size: 6091 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4160 4192 5673 5705 4160 Size: 32 1481 32 4546 Fragments arranged by size: 4546 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2701 2860 2902 3343 2701 Size: 159 42 441 5449 Fragments arranged by size: 5449 441 159 42 PshAI GACnn'nnGTC Cuts at: 1613 2468 1613 Size: 855 5236 PsiI TTA'TAA Cuts at: 3661 3661 Size: 6091 PstI C_TGCA'G Cuts at: 830 2419 2760 3443 830 Size: 1589 341 683 3478 Fragments arranged by size: 3478 1589 683 341 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6091 PvuII CAG'CTG Cuts at: 2493 2587 2650 2959 2493 Size: 94 63 309 5625 Fragments arranged by size: 5625 309 94 63 SacI G_AGCT'C Cuts at: 3017 3017 Size: 6091 SalI G'TCGA_C Cuts at: 1936 3228 3433 1936 Size: 1292 205 4594 Fragments arranged by size: 4594 1292 205 SbfI CC_TGCA'GG Cuts at: 2760 3443 2760 Size: 683 5408 ScaI AGT'ACT Cuts at: 4339 6051 4339 Size: 1712 4379 SfcI C'TryA_G Cuts at: 826 1045 2415 2756 3439 4574 5255 5446 826 Size: 219 1370 341 683 1135 681 191 1471 Fragments arranged by size: 1471 1370 1135 683 681 341 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2573 2759 2573 Size: 186 5905 SfoI GGC'GCC Cuts at: 2936 3281 2936 Size: 345 5746 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6091 SmaI CCC'GGG Cuts at: 1872 2219 3418 1872 Size: 347 1199 4545 Fragments arranged by size: 4545 1199 347 SmlI C'TyrA_G Cuts at: 820 1017 1943 2882 4195 5096 5358 820 Size: 197 926 939 1313 901 262 1553 Fragments arranged by size: 1553 1313 939 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3882 493 Size: 3389 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6091 SphI G_CATG'C Cuts at: 1132 3449 1132 Size: 2317 3774 SspI AAT'ATT Cuts at: 5 52 4015 5 Size: 47 3963 2081 Fragments arranged by size: 3963 2081 47 StuI AGG'CCT Cuts at: 1517 2117 1517 Size: 600 5491 StyI C'CwwG_G Cuts at: 513 1065 2004 2118 2391 2685 3111 3530 513 Size: 552 939 114 273 294 426 419 3074 Fragments arranged by size: 3074 939 552 426 419 294 273 114 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2338 2607 4118 4135 4288 4473 4918 Size: 1255 758 269 1511 17 153 185 445 Cuts at: 4918 325 Size: 1498 Fragments arranged by size: 1511 1498 1255 758 445 269 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4337 6049 96 Size: 274 80 33 51 1443 2360 1712 138 Fragments arranged by size: 2360 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3163 3195 3243 3275 6051 6083 3163 Size: 32 48 32 2776 32 3171 Fragments arranged by size: 3171 2776 48 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6091 XbaI T'CTAG_A Cuts at: 3427 3427 Size: 6091 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6091 XmaI C'CCGG_G Cuts at: 1870 2217 3416 1870 Size: 347 1199 4545 Fragments arranged by size: 4545 1199 347 XmnI GAAnn'nnTTC Cuts at: 2171 4220 2171 Size: 2049 4042 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3899 276 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SrfI SwaI XcmI