(Circular) (Six-base) MAPSORT of: pFN21AB5590.seq Check: 6370 from: 1 to: 5752 pFN21AB5590 5752 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3562 278 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3095 1937 Size: 1158 4594 Acc65I G'GTAC_C Cuts at: 3073 3073 Size: 5752 AclI AA'CG_TT Cuts at: 1563 3879 4252 1563 Size: 2316 373 3063 Fragments arranged by size: 3063 2316 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3815 5199 5467 1632 Size: 2183 1384 268 1917 Fragments arranged by size: 2183 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5555 AflIII A'CryG_T Cuts at: 1203 2562 4651 5628 1203 Size: 1359 2089 977 1327 Fragments arranged by size: 2089 1359 1327 977 AgeI A'CCGG_T Cuts at: 4583 4583 Size: 5752 AhdI GACnn_n'nnGTC Cuts at: 4481 4481 Size: 5752 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5752 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5752 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2614 2646 2614 Size: 32 5720 AlwNI CAG_nnn'CTG Cuts at: 3495 5067 3495 Size: 1572 4180 ApaI G_GGCC'C Cuts at: 2027 2027 Size: 5752 ApaLI G'TGCA_C Cuts at: 2206 2287 2374 3809 4965 2206 Size: 81 87 1435 1156 2993 Fragments arranged by size: 2993 1435 1156 87 81 ApoI r'AATT_y Cuts at: 1493 2849 3061 3287 3439 5548 1493 Size: 1356 212 226 152 2109 1697 Fragments arranged by size: 2109 1697 1356 226 212 152 AseI AT'TA_AT Cuts at: 160 1032 4306 160 Size: 872 3274 1606 Fragments arranged by size: 3274 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 AvaI C'yCGr_G Cuts at: 1870 1943 2455 2474 2661 3077 1870 Size: 73 512 19 187 416 4545 Fragments arranged by size: 4545 512 416 187 73 19 BamHI G'GATC_C Cuts at: 3082 3449 3082 Size: 367 5385 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2106 2122 3073 4528 618 Size: 325 217 627 319 16 951 1455 1842 Fragments arranged by size: 1842 1455 951 627 325 319 217 16 BanII G_rGCy'C Cuts at: 1391 2027 2511 3071 1391 Size: 636 484 560 4072 Fragments arranged by size: 4072 636 560 484 BbeI G_GCGC'C Cuts at: 2126 2126 Size: 5752 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2565 2840 928 Size: 971 666 275 3840 Fragments arranged by size: 3840 971 666 275 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3943 3977 1903 Size: 34 2006 34 3678 Fragments arranged by size: 3678 2006 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3644 4854 3644 Size: 1210 4542 BclI T'GATC_A Cuts at: 1548 1548 Size: 5752 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2139 2543 2931 Size: 107 122 71 631 605 467 404 388 Cuts at: 2931 4363 136 Size: 1432 1525 Fragments arranged by size: 1525 1432 631 605 467 404 388 122 107 71 BglII A'GATC_T Cuts at: 5747 5747 Size: 5752 BlpI GC'TnA_GC Cuts at: 3169 3169 Size: 5752 Bme1580I G_kGCm'C Cuts at: 1790 2027 2111 2210 2291 2378 2927 3813 4969 Size: 237 84 99 81 87 549 886 1156 Cuts at: 4969 1790 Size: 2573 Fragments arranged by size: 2573 1156 886 549 237 99 87 84 81 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5752 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3483 4441 442 Size: 416 53 83 399 24 2066 958 1753 Fragments arranged by size: 2066 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5752 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4412 1239 Size: 3173 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3877 4742 5040 3877 Size: 865 298 4589 Fragments arranged by size: 4589 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4415 882 Size: 617 2916 2219 Fragments arranged by size: 2916 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2379 3543 493 Size: 711 1175 1164 2702 Fragments arranged by size: 2702 1175 1164 711 BsaBI GATnn'nnATC Cuts at: 2651 2960 5746 2651 Size: 309 2786 2657 Fragments arranged by size: 2786 2657 309 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2123 3559 3941 4576 Size: 53 83 186 936 590 1436 382 635 Cuts at: 4576 275 Size: 1451 Fragments arranged by size: 1451 1436 936 635 590 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4185 4583 4857 5004 5415 4185 Size: 398 274 147 411 4522 Fragments arranged by size: 4522 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2243 2273 2483 2513 2614 Size: 30 165 30 840 30 210 30 101 Cuts at: 2614 2644 2666 2696 2906 2936 1178 Size: 30 22 30 210 30 3994 Fragments arranged by size: 3994 840 210 210 165 101 30 30 30 30 30 30 30 22 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 2473 2504 1186 Size: 856 431 31 4434 Fragments arranged by size: 4434 856 431 31 BseYI C'CCAG_C Cuts at: 2194 2435 4955 5451 2194 Size: 241 2520 496 2495 Fragments arranged by size: 2520 2495 496 241 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2434 2806 2866 1163 Size: 573 698 372 60 4049 Fragments arranged by size: 4049 698 573 372 60 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2072 2076 2875 3202 3963 4991 Size: 275 186 71 4 799 327 761 1028 Cuts at: 4991 1540 Size: 2301 Fragments arranged by size: 2301 1028 799 761 327 275 186 71 4 BsiHKAI G_wGCw'C Cuts at: 2210 2291 2308 2378 3813 3898 4969 2210 Size: 81 17 70 1435 85 1071 2993 Fragments arranged by size: 2993 1435 1071 85 81 70 17 BsmI GAATG_Cn' Cuts at: 3263 3356 3263 Size: 93 5659 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2027 2111 2210 2291 2308 2378 2511 Size: 399 237 84 99 81 17 70 133 Cuts at: 2511 2927 3071 3813 3898 4969 1391 Size: 416 144 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 416 399 237 144 133 99 85 84 81 70 17 BspEI T'CCGG_A Cuts at: 5415 5415 Size: 5752 BspHI T'CATG_A Cuts at: 2940 3641 5371 2940 Size: 701 1730 3321 Fragments arranged by size: 3321 1730 701 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3107 844 Size: 2263 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3639 1394 Size: 429 1816 3507 Fragments arranged by size: 3507 1816 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4247 4421 65 Size: 1183 2999 174 1396 Fragments arranged by size: 2999 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2067 2071 2103 2119 2908 4396 4583 Size: 110 257 4 32 16 789 1488 187 Cuts at: 4583 1700 Size: 2869 Fragments arranged by size: 2869 1488 789 257 187 110 32 16 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5752 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5752 BssSI C'ACGA_G Cuts at: 2150 3812 4824 2150 Size: 1662 1012 3078 Fragments arranged by size: 3078 1662 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2769 794 Size: 1975 3777 BstBI TT'CG_AA Cuts at: 4556 4556 Size: 5752 BstEII G'GTnAC_C Cuts at: 1184 2665 1184 Size: 1481 4271 BstXI CCAn_nnnn'nTGG Cuts at: 2194 2602 2194 Size: 408 5344 BstYI r'GATC_y Cuts at: 1972 3082 3449 3834 3851 5303 5389 5401 5747 Size: 1110 367 385 17 1452 86 12 346 Cuts at: 5747 1972 Size: 1977 Fragments arranged by size: 1977 1452 1110 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2523 513 Size: 552 1458 3742 Fragments arranged by size: 3742 1458 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5013 Fragments arranged by size: 5013 683 56 BtsI GCAGTG_nn' Cuts at: 3268 4062 4082 3268 Size: 794 20 4938 Fragments arranged by size: 4938 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5752 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2623 2658 529 Size: 35 2059 35 3623 Fragments arranged by size: 3623 2059 35 35 DraI TTT'AAA Cuts at: 3056 3056 Size: 5752 DraIII CAC_nnn'GTG Cuts at: 2619 2619 Size: 5752 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2936 4759 809 Size: 507 1620 1823 1802 Fragments arranged by size: 1823 1802 1620 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2069 2073 2080 Size: 54 1425 50 275 18 239 4 7 Cuts at: 2080 2265 2872 3199 4088 5717 8 Size: 185 607 327 889 1629 43 Fragments arranged by size: 1629 1425 889 607 327 275 239 185 54 50 43 18 7 4 EagI C'GGCC_G Cuts at: 1537 1812 2069 2073 2872 3199 1537 Size: 275 257 4 799 327 4090 Fragments arranged by size: 4090 799 327 275 257 4 EarI CTCTTCn'nnn_ Cuts at: 1353 2249 2739 3682 1353 Size: 896 490 943 3423 Fragments arranged by size: 3423 943 896 490 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4339 4713 4859 387 Size: 1064 138 2750 374 146 1280 Fragments arranged by size: 2750 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3815 4412 5199 5467 1239 Size: 393 2183 597 787 268 1524 Fragments arranged by size: 2183 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2455 2686 2455 Size: 231 5521 EcoO109I rG'GnC_Cy Cuts at: 2024 2239 2024 Size: 215 5537 EcoRI G'AATT_C Cuts at: 3061 3439 3061 Size: 378 5374 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2260 2260 Size: 5752 FseI GG_CCGG'CC Cuts at: 2075 2075 Size: 5752 FspI TGC'GCA Cuts at: 4258 4258 Size: 5752 HaeII r_GCGC'y Cuts at: 1678 2126 2628 4899 1678 Size: 448 502 2271 2531 Fragments arranged by size: 2531 2271 502 448 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2243 2275 2666 2698 4393 Size: 32 135 32 671 32 391 32 1695 Cuts at: 4393 4425 4467 4499 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1695 671 391 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3096 3342 669 Size: 988 281 1158 246 3079 Fragments arranged by size: 3079 1158 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5752 HpaI GTT'AAC Cuts at: 3342 3342 Size: 5752 KasI G'GCGC_C Cuts at: 2122 2122 Size: 5752 KpnI G_GTAC'C Cuts at: 3077 3077 Size: 5752 MfeI C'AATT_G Cuts at: 3351 3351 Size: 5752 MluI A'CGCG_T Cuts at: 5628 5628 Size: 5752 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4866 5050 1305 Size: 3561 184 2007 Fragments arranged by size: 3561 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3930 Fragments arranged by size: 3930 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2828 3710 4069 4228 518 Size: 648 1662 882 359 159 2042 Fragments arranged by size: 2042 1662 882 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2094 2194 2340 2361 3168 3845 4993 5238 Size: 488 100 146 21 807 677 1148 245 Cuts at: 5238 1606 Size: 2120 Fragments arranged by size: 2120 1148 807 677 488 245 146 100 21 NaeI GCC'GGC Cuts at: 2069 2073 2121 2069 Size: 4 48 5700 Fragments arranged by size: 5700 48 4 NarI GG'CG_CC Cuts at: 2123 2123 Size: 5752 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5200 NdeI CA'TA_TG Cuts at: 387 387 Size: 5752 NgoMIV G'CCGG_C Cuts at: 2067 2071 2119 2067 Size: 4 48 5700 Fragments arranged by size: 5700 48 4 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5752 NotI GC'GGCC_GC Cuts at: 3199 3199 Size: 5752 NspI r_CATG'y Cuts at: 1132 1229 2407 3110 4655 1132 Size: 97 1178 703 1545 2229 Fragments arranged by size: 2229 1545 1178 703 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5752 PciI A'CATG_T Cuts at: 4651 4651 Size: 5752 PflMI CCAn_nnn'nTGG Cuts at: 1108 2524 2619 1108 Size: 1416 95 4241 Fragments arranged by size: 4241 1416 95 PfoI T'CCnGG_A Cuts at: 2992 2992 Size: 5752 PmeI GTTT'AAAC Cuts at: 3056 3056 Size: 5752 PmlI CAC'GTG Cuts at: 2379 2379 Size: 5752 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2614 2646 3821 3853 5334 5366 2614 Size: 32 1175 32 1481 32 3000 Fragments arranged by size: 3000 1481 1175 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2427 1613 Size: 814 4938 PsiI TTA'TAA Cuts at: 3322 3322 Size: 5752 PspOMI G'GGCC_C Cuts at: 2023 2023 Size: 5752 PstI C_TGCA'G Cuts at: 830 2066 2093 2690 3104 830 Size: 1236 27 597 414 3478 Fragments arranged by size: 3478 1236 597 414 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5752 PvuII CAG'CTG Cuts at: 2194 2361 2194 Size: 167 5585 SalI G'TCGA_C Cuts at: 1936 3094 1936 Size: 1158 4594 SbfI CC_TGCA'GG Cuts at: 2690 3104 2690 Size: 414 5338 ScaI AGT'ACT Cuts at: 4000 5712 4000 Size: 1712 4040 SfcI C'TryA_G Cuts at: 826 1045 2062 2089 2686 3025 3100 4235 4916 Size: 219 1017 27 597 339 75 1135 681 Cuts at: 4916 5107 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1017 681 597 339 219 191 75 27 SfoI GGC'GCC Cuts at: 2124 2124 Size: 5752 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5752 SmaI CCC'GGG Cuts at: 1872 2476 2663 3079 1872 Size: 604 187 416 4545 Fragments arranged by size: 4545 604 416 187 SmlI C'TyrA_G Cuts at: 820 1017 1943 3856 4757 5019 820 Size: 197 926 1913 901 262 1553 Fragments arranged by size: 1913 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3543 493 Size: 3050 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5752 SphI G_CATG'C Cuts at: 1132 2407 3110 1132 Size: 1275 703 3774 Fragments arranged by size: 3774 1275 703 SspI AAT'ATT Cuts at: 5 52 3676 5 Size: 47 3624 2081 Fragments arranged by size: 3624 2081 47 StuI AGG'CCT Cuts at: 1517 2725 1517 Size: 1208 4544 StyI C'CwwG_G Cuts at: 513 1065 2511 3191 513 Size: 552 1446 680 3074 Fragments arranged by size: 3074 1446 680 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2485 3779 3796 3949 4134 4579 325 Size: 1255 905 1294 17 153 185 445 1498 Fragments arranged by size: 1498 1294 1255 905 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3021 3998 5710 Size: 274 80 33 51 1443 1044 977 1712 Cuts at: 5710 96 Size: 138 Fragments arranged by size: 1712 1443 1044 977 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2483 2515 2906 2938 5712 5744 2483 Size: 32 391 32 2774 32 2491 Fragments arranged by size: 2774 2491 391 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5752 XbaI T'CTAG_A Cuts at: 3088 3088 Size: 5752 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2525 2908 2525 Size: 383 5369 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5752 XmaI C'CCGG_G Cuts at: 1870 2474 2661 3077 1870 Size: 604 187 416 4545 Fragments arranged by size: 4545 604 416 187 XmnI GAAnn'nnTTC Cuts at: 3881 3881 Size: 5752 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3560 276 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FspAI NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI