(Circular) (Six-base) MAPSORT of: pFN21AB5574.seq Check: 5783 from: 1 to: 5341 pFN21AB5574 5341 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3151 278 Size: 53 83 186 936 1615 2468 Fragments arranged by size: 2468 1615 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2256 2684 1937 Size: 319 428 4594 Fragments arranged by size: 4594 428 319 Acc65I G'GTAC_C Cuts at: 2662 2662 Size: 5341 AclI AA'CG_TT Cuts at: 1563 3468 3841 1563 Size: 1905 373 3063 Fragments arranged by size: 3063 1905 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2627 3404 4788 5056 1632 Size: 995 777 1384 268 1917 Fragments arranged by size: 1917 1384 995 777 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5144 AflIII A'CryG_T Cuts at: 1203 4240 5217 1203 Size: 3037 977 1327 Fragments arranged by size: 3037 1327 977 AgeI A'CCGG_T Cuts at: 4172 4172 Size: 5341 AhdI GACnn_n'nnGTC Cuts at: 4070 4070 Size: 5341 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5341 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5341 AlwNI CAG_nnn'CTG Cuts at: 2552 3084 4656 2552 Size: 532 1572 3237 Fragments arranged by size: 3237 1572 532 ApaLI G'TGCA_C Cuts at: 2120 3398 4554 2120 Size: 1278 1156 2907 Fragments arranged by size: 2907 1278 1156 ApoI r'AATT_y Cuts at: 1493 2650 2876 3028 5137 1493 Size: 1157 226 152 2109 1697 Fragments arranged by size: 2109 1697 1157 226 152 AseI AT'TA_AT Cuts at: 160 1032 3895 160 Size: 872 2863 1606 Fragments arranged by size: 2863 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5341 AvaI C'yCGr_G Cuts at: 1870 1943 2162 2182 2666 1870 Size: 73 219 20 484 4545 Fragments arranged by size: 4545 484 219 73 20 BamHI G'GATC_C Cuts at: 2671 3038 2671 Size: 367 4974 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2662 4117 618 Size: 325 217 627 875 1455 1842 Fragments arranged by size: 1842 1455 875 627 325 217 BanII G_rGCy'C Cuts at: 1391 2660 1391 Size: 1269 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2023 928 Size: 971 124 4246 Fragments arranged by size: 4246 971 124 BbvCI CC'TCA_GC Cuts at: 2196 2196 Size: 5341 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3532 3566 1903 Size: 34 1595 34 3678 Fragments arranged by size: 3678 1595 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3233 4443 3233 Size: 1210 4131 BclI T'GATC_A Cuts at: 1548 1548 Size: 5341 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3952 136 Size: 107 122 71 631 605 2280 1525 Fragments arranged by size: 2280 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5336 5336 Size: 5341 BlpI GC'TnA_GC Cuts at: 2758 2758 Size: 5341 Bme1580I G_kGCm'C Cuts at: 1790 2124 3402 4558 1790 Size: 334 1278 1156 2573 Fragments arranged by size: 2573 1278 1156 334 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5341 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3072 4030 442 Size: 416 53 83 399 24 1655 958 1753 Fragments arranged by size: 1753 1655 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5341 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2173 2255 4001 1239 Size: 934 82 1746 2579 Fragments arranged by size: 2579 1746 934 82 Bpu10I CC'TnA_GC Cuts at: 2196 2196 Size: 5341 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2513 3466 4331 4629 2513 Size: 953 865 298 3225 Fragments arranged by size: 3225 953 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4004 882 Size: 617 2505 2219 Fragments arranged by size: 2505 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2119 2361 3132 493 Size: 711 915 242 771 2702 Fragments arranged by size: 2702 915 771 711 242 BsaBI GATnn'nnATC Cuts at: 5335 5335 Size: 5341 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2052 2188 3148 3530 Size: 53 83 186 936 519 136 960 382 Cuts at: 3530 4165 275 Size: 635 1451 Fragments arranged by size: 1451 960 936 635 519 382 186 136 83 53 BsaWI w'CCGG_w Cuts at: 3774 4172 4446 4593 5004 3774 Size: 398 274 147 411 4111 Fragments arranged by size: 4111 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5116 Fragments arranged by size: 5116 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2014 1186 Size: 828 4513 BseYI C'CCAG_C Cuts at: 2104 2249 2427 4544 5040 2104 Size: 145 178 2117 496 2405 Fragments arranged by size: 2405 2117 496 178 145 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2264 2565 1163 Size: 573 528 301 3939 Fragments arranged by size: 3939 573 528 301 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2599 2791 3552 4580 1540 Size: 275 186 598 192 761 1028 2301 Fragments arranged by size: 2301 1028 761 598 275 192 186 BsiHKAI G_wGCw'C Cuts at: 2124 3402 3487 4558 2124 Size: 1278 85 1071 2907 Fragments arranged by size: 2907 1278 1071 85 BsmI GAATG_Cn' Cuts at: 2852 2945 2852 Size: 93 5248 BsmBI CGTCTCn'nnnn_ Cuts at: 2497 2497 Size: 5341 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 2660 3402 3487 4558 1391 Size: 399 334 536 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 536 399 334 85 BspEI T'CCGG_A Cuts at: 5004 5004 Size: 5341 BspHI T'CATG_A Cuts at: 3230 4960 3230 Size: 1730 3611 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2696 844 Size: 1852 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2064 2562 3228 1394 Size: 429 241 498 666 3507 Fragments arranged by size: 3507 666 498 429 241 BsrDI GCAATG_nn' Cuts at: 65 1248 3836 4010 65 Size: 1183 2588 174 1396 Fragments arranged by size: 2588 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2141 2176 3985 4172 1700 Size: 110 331 35 1809 187 2869 Fragments arranged by size: 2869 1809 331 187 110 35 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5341 BssHII G'CGCG_C Cuts at: 1925 2137 2157 1925 Size: 212 20 5109 Fragments arranged by size: 5109 212 20 BssSI C'ACGA_G Cuts at: 2450 3401 4413 2450 Size: 951 1012 3378 Fragments arranged by size: 3378 1012 951 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5341 BstBI TT'CG_AA Cuts at: 4145 4145 Size: 5341 BstEII G'GTnAC_C Cuts at: 1184 2308 1184 Size: 1124 4217 BstXI CCAn_nnnn'nTGG Cuts at: 2104 2104 Size: 5341 BstYI r'GATC_y Cuts at: 1972 2671 3038 3423 3440 4892 4978 4990 5336 Size: 699 367 385 17 1452 86 12 346 Cuts at: 5336 1972 Size: 1977 Fragments arranged by size: 1977 1452 699 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2328 2394 2406 2436 513 Size: 552 939 324 66 12 30 3418 Fragments arranged by size: 3418 939 552 324 66 30 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2241 487 Size: 683 56 1015 3587 Fragments arranged by size: 3587 1015 683 56 BtsI GCAGTG_nn' Cuts at: 2857 3651 3671 2857 Size: 794 20 4527 Fragments arranged by size: 4527 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5341 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5306 DraI TTT'AAA Cuts at: 2645 2645 Size: 5341 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4348 809 Size: 507 3032 1802 Fragments arranged by size: 3032 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2325 2335 2391 Size: 54 1425 50 275 18 495 10 56 Cuts at: 2391 2403 2596 2788 3677 5306 8 Size: 12 193 192 889 1629 43 Fragments arranged by size: 1629 1425 889 495 275 193 192 56 54 50 43 18 12 10 EagI C'GGCC_G Cuts at: 1537 1812 2596 2788 1537 Size: 275 784 192 4090 Fragments arranged by size: 4090 784 275 192 EarI CTCTTCn'nnn_ Cuts at: 1353 3271 1353 Size: 1918 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2526 3928 4302 4448 387 Size: 1064 138 937 1402 374 146 1280 Fragments arranged by size: 1402 1280 1064 937 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2173 2255 2627 3404 4001 4788 5056 Size: 393 541 82 372 777 597 787 268 Cuts at: 5056 1239 Size: 1524 Fragments arranged by size: 1524 787 777 597 541 393 372 268 82 EcoNI CCTnn'n_nnAGG Cuts at: 2195 2195 Size: 5341 EcoRI G'AATT_C Cuts at: 2650 3028 2650 Size: 378 4963 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2632 2632 Size: 5341 FspI TGC'GCA Cuts at: 2477 3847 2477 Size: 1370 3971 HaeII r_GCGC'y Cuts at: 1678 2023 2105 4488 1678 Size: 345 82 2383 2531 Fragments arranged by size: 2531 2383 345 82 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3982 4014 4056 4088 1373 Size: 32 135 32 2410 32 42 32 2626 Fragments arranged by size: 2626 2410 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2685 2931 669 Size: 988 281 747 246 3079 Fragments arranged by size: 3079 988 747 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5341 HpaI GTT'AAC Cuts at: 2931 2931 Size: 5341 KpnI G_GTAC'C Cuts at: 2666 2666 Size: 5341 MfeI C'AATT_G Cuts at: 2940 2940 Size: 5341 MluI A'CGCG_T Cuts at: 5217 5217 Size: 5341 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4455 4639 1305 Size: 3150 184 2007 Fragments arranged by size: 3150 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2337 10 Size: 54 1425 343 505 3014 Fragments arranged by size: 3014 1425 505 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3299 3658 3817 518 Size: 648 843 1290 359 159 2042 Fragments arranged by size: 2042 1290 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2278 2396 2757 3434 4582 4827 1606 Size: 672 118 361 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 672 361 245 118 NaeI GCC'GGC Cuts at: 2143 2143 Size: 5341 NcoI C'CATG_G Cuts at: 513 1065 2004 2436 513 Size: 552 939 432 3418 Fragments arranged by size: 3418 939 552 432 NdeI CA'TA_TG Cuts at: 387 387 Size: 5341 NgoMIV G'CCGG_C Cuts at: 2141 2141 Size: 5341 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5341 NotI GC'GGCC_GC Cuts at: 2788 2788 Size: 5341 NspI r_CATG'y Cuts at: 1132 1229 2699 4244 1132 Size: 97 1470 1545 2229 Fragments arranged by size: 2229 1545 1470 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5341 PciI A'CATG_T Cuts at: 4240 4240 Size: 5341 PflMI CCAn_nnn'nTGG Cuts at: 1108 2572 1108 Size: 1464 3877 PfoI T'CCnGG_A Cuts at: 2150 2150 Size: 5341 PmeI GTTT'AAAC Cuts at: 2645 2645 Size: 5341 PmlI CAC'GTG Cuts at: 2361 2361 Size: 5341 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3410 3442 4923 4955 3410 Size: 32 1481 32 3796 Fragments arranged by size: 3796 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2522 1613 Size: 909 4432 PsiI TTA'TAA Cuts at: 2911 2911 Size: 5341 PstI C_TGCA'G Cuts at: 830 2295 2353 2591 2693 830 Size: 1465 58 238 102 3478 Fragments arranged by size: 3478 1465 238 102 58 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5341 PvuII CAG'CTG Cuts at: 2278 2278 Size: 5341 SacII CC_GC'GG Cuts at: 2397 2397 Size: 5341 SalI G'TCGA_C Cuts at: 1936 2683 1936 Size: 747 4594 SbfI CC_TGCA'GG Cuts at: 2693 2693 Size: 5341 ScaI AGT'ACT Cuts at: 3589 5301 3589 Size: 1712 3629 SfcI C'TryA_G Cuts at: 826 1045 2291 2349 2587 2689 3824 4505 4696 Size: 219 1246 58 238 102 1135 681 191 Cuts at: 4696 826 Size: 1471 Fragments arranged by size: 1471 1246 1135 681 238 219 191 102 58 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5341 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5341 SmaI CCC'GGG Cuts at: 1872 2184 2668 1872 Size: 312 484 4545 Fragments arranged by size: 4545 484 312 SmlI C'TyrA_G Cuts at: 820 1017 1943 2528 3445 4346 4608 820 Size: 197 926 585 917 901 262 1553 Fragments arranged by size: 1553 926 917 901 585 262 197 SnaBI TAC'GTA Cuts at: 493 3132 493 Size: 2639 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5341 SphI G_CATG'C Cuts at: 1132 2699 1132 Size: 1567 3774 SspI AAT'ATT Cuts at: 5 52 3265 5 Size: 47 3213 2081 Fragments arranged by size: 3213 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5341 StyI C'CwwG_G Cuts at: 513 1065 2004 2097 2436 2780 513 Size: 552 939 93 339 344 3074 Fragments arranged by size: 3074 939 552 344 339 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2017 2327 3368 3385 3538 3723 4168 Size: 1255 437 310 1041 17 153 185 445 Cuts at: 4168 325 Size: 1498 Fragments arranged by size: 1498 1255 1041 445 437 310 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3587 5299 96 Size: 274 80 33 51 1443 1610 1712 138 Fragments arranged by size: 1712 1610 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5301 5333 5301 Size: 32 5309 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5341 XbaI T'CTAG_A Cuts at: 2677 2677 Size: 5341 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5341 XmaI C'CCGG_G Cuts at: 1870 2182 2666 1870 Size: 312 484 4545 Fragments arranged by size: 4545 484 312 XmnI GAAnn'nnTTC Cuts at: 3470 3470 Size: 5341 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3149 276 Size: 53 83 186 936 1615 2468 Fragments arranged by size: 2468 1615 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI