(Circular) (Six-base) MAPSORT of: pFN21AB5569.seq Check: 1423 from: 1 to: 6112 pFN21AB5569 6112 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3922 278 Size: 53 83 186 936 2386 2468 Fragments arranged by size: 2468 2386 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3455 1937 Size: 1518 4594 Acc65I G'GTAC_C Cuts at: 3433 3433 Size: 6112 AclI AA'CG_TT Cuts at: 1563 4239 4612 1563 Size: 2676 373 3063 Fragments arranged by size: 3063 2676 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2156 2276 2713 4175 5559 5827 1632 Size: 524 120 437 1462 1384 268 1917 Fragments arranged by size: 1917 1462 1384 524 437 268 120 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5915 AflIII A'CryG_T Cuts at: 1203 5011 5988 1203 Size: 3808 977 1327 Fragments arranged by size: 3808 1327 977 AgeI A'CCGG_T Cuts at: 4943 4943 Size: 6112 AhdI GACnn_n'nnGTC Cuts at: 4841 4841 Size: 6112 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6112 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3153 1184 Size: 1969 4143 AlwNI CAG_nnn'CTG Cuts at: 2246 3855 5427 2246 Size: 1609 1572 2931 Fragments arranged by size: 2931 1609 1572 ApaLI G'TGCA_C Cuts at: 4169 5325 4169 Size: 1156 4956 ApoI r'AATT_y Cuts at: 1493 2210 3002 3341 3363 3421 3647 3799 5908 Size: 717 792 339 22 58 226 152 2109 Cuts at: 5908 1493 Size: 1697 Fragments arranged by size: 2109 1697 792 717 339 226 152 58 22 AseI AT'TA_AT Cuts at: 160 1032 4666 160 Size: 872 3634 1606 Fragments arranged by size: 3634 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 AvaI C'yCGr_G Cuts at: 1870 1943 3437 1870 Size: 73 1494 4545 Fragments arranged by size: 4545 1494 73 BamHI G'GATC_C Cuts at: 3226 3442 3809 3226 Size: 216 367 5529 Fragments arranged by size: 5529 367 216 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3433 4888 618 Size: 325 217 627 1646 1455 1842 Fragments arranged by size: 1842 1646 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2946 3431 1391 Size: 1555 485 4072 Fragments arranged by size: 4072 1555 485 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5141 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4303 4337 1903 Size: 34 50 34 2282 34 3678 Fragments arranged by size: 3678 2282 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3074 4004 5214 3074 Size: 930 1210 3972 Fragments arranged by size: 3972 1210 930 BclI T'GATC_A Cuts at: 1548 1548 Size: 6112 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2983 4723 136 Size: 107 122 71 631 605 1311 1740 1525 Fragments arranged by size: 1740 1525 1311 631 605 122 107 71 BglII A'GATC_T Cuts at: 6107 6107 Size: 6112 BlpI GC'TnA_GC Cuts at: 3529 3529 Size: 6112 Bme1580I G_kGCm'C Cuts at: 1790 4173 5329 1790 Size: 2383 1156 2573 Fragments arranged by size: 2573 2383 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6112 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2174 2241 3058 Size: 416 53 83 399 24 757 67 817 Cuts at: 3058 3843 4801 442 Size: 785 958 1753 Fragments arranged by size: 1753 958 817 785 757 416 399 83 67 53 24 BmtI G_CTAG'C Cuts at: 1056 2750 1056 Size: 1694 4418 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2446 2556 3217 3359 4772 1239 Size: 1207 110 661 142 1413 2579 Fragments arranged by size: 2579 1413 1207 661 142 110 Bpu10I CC'TnA_GC Cuts at: 2751 2751 Size: 6112 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4237 5102 5400 4237 Size: 865 298 4949 Fragments arranged by size: 4949 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2464 4775 882 Size: 617 965 2311 2219 Fragments arranged by size: 2311 2219 965 617 BsaAI yAC'GTr Cuts at: 493 1204 3903 493 Size: 711 2699 2702 Fragments arranged by size: 2702 2699 711 BsaBI GATnn'nnATC Cuts at: 6106 6106 Size: 6112 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3919 4301 4936 275 Size: 53 83 186 936 2386 382 635 1451 Fragments arranged by size: 2386 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3024 4545 4943 5217 5364 5775 3024 Size: 1521 398 274 147 411 3361 Fragments arranged by size: 3361 1521 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2158 2188 1178 Size: 30 165 30 755 30 5102 Fragments arranged by size: 5102 755 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6112 BseYI C'CCAG_C Cuts at: 2065 2328 5315 5811 2065 Size: 263 2987 496 2366 Fragments arranged by size: 2987 2366 496 263 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5539 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3562 4323 5351 1540 Size: 275 186 1561 761 1028 2301 Fragments arranged by size: 2301 1561 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2946 4173 4258 5329 2946 Size: 1227 85 1071 3729 Fragments arranged by size: 3729 1227 1071 85 BsmI GAATG_Cn' Cuts at: 3623 3716 3623 Size: 93 6019 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2946 3431 4173 4258 5329 1391 Size: 399 1156 485 742 85 1071 2174 Fragments arranged by size: 2174 1156 1071 742 485 399 85 BspEI T'CCGG_A Cuts at: 5775 5775 Size: 6112 BspHI T'CATG_A Cuts at: 2481 2656 3294 4001 5731 2481 Size: 175 638 707 1730 2862 Fragments arranged by size: 2862 1730 707 638 175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3467 844 Size: 2623 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3999 1394 Size: 429 2176 3507 Fragments arranged by size: 3507 2176 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4607 4781 65 Size: 1183 3359 174 1396 Fragments arranged by size: 3359 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4756 4943 1700 Size: 110 2946 187 2869 Fragments arranged by size: 2946 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6112 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6112 BssSI C'ACGA_G Cuts at: 4172 5184 4172 Size: 1012 5100 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6112 BstBI TT'CG_AA Cuts at: 4916 4916 Size: 6112 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6112 BstXI CCAn_nnnn'nTGG Cuts at: 2675 2675 Size: 6112 BstYI r'GATC_y Cuts at: 1972 3226 3442 3809 4194 4211 5663 5749 5761 Size: 1254 216 367 385 17 1452 86 12 Cuts at: 5761 6107 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1254 385 367 346 216 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5560 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5373 Fragments arranged by size: 5373 683 56 BtsI GCAGTG_nn' Cuts at: 2021 3628 4422 4442 2021 Size: 1607 794 20 3691 Fragments arranged by size: 3691 1607 794 20 ClaI AT'CG_AT Cuts at: 1554 3135 1554 Size: 1581 4531 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6077 DraI TTT'AAA Cuts at: 2896 3416 2896 Size: 520 5592 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5119 809 Size: 507 3803 1802 Fragments arranged by size: 3803 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2665 2966 3271 Size: 54 1425 50 275 18 835 301 305 Cuts at: 3271 3559 4448 6077 8 Size: 288 889 1629 43 Fragments arranged by size: 1629 1425 889 835 305 301 288 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3559 1537 Size: 275 1747 4090 Fragments arranged by size: 4090 1747 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3353 4042 1353 Size: 2000 689 3423 Fragments arranged by size: 3423 2000 689 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4699 5073 5219 387 Size: 1064 138 3110 374 146 1280 Fragments arranged by size: 3110 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2944 2944 Size: 6112 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2276 2446 2556 2713 3217 3359 Size: 393 524 120 170 110 157 504 142 Cuts at: 3359 4175 4772 5559 5827 1239 Size: 816 597 787 268 1524 Fragments arranged by size: 1524 816 787 597 524 504 393 268 170 157 142 120 110 EcoNI CCTnn'n_nnAGG Cuts at: 2312 2312 Size: 6112 EcoRI G'AATT_C Cuts at: 2210 3421 3799 2210 Size: 1211 378 4523 Fragments arranged by size: 4523 1211 378 EcoRV GAT'ATC Cuts at: 2487 2698 2487 Size: 211 5901 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2869 2869 Size: 6112 FspI TGC'GCA Cuts at: 2915 4618 2915 Size: 1703 4409 FspAI rTGC'GCAy Cuts at: 2915 2915 Size: 6112 HaeII r_GCGC'y Cuts at: 1678 5259 1678 Size: 3581 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3006 3038 4753 4785 4827 Size: 32 135 32 1434 32 1715 32 42 Cuts at: 4827 4859 1373 Size: 32 2626 Fragments arranged by size: 2626 1715 1434 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3456 3702 669 Size: 988 281 1518 246 3079 Fragments arranged by size: 3079 1518 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6112 HpaI GTT'AAC Cuts at: 3702 3702 Size: 6112 KpnI G_GTAC'C Cuts at: 3437 3437 Size: 6112 MfeI C'AATT_G Cuts at: 3711 3711 Size: 6112 MluI A'CGCG_T Cuts at: 5988 5988 Size: 6112 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2443 5226 5410 1305 Size: 1138 2783 184 2007 Fragments arranged by size: 2783 2007 1138 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2968 3273 10 Size: 54 1425 343 1136 305 2849 Fragments arranged by size: 2849 1425 1136 343 305 54 MslI CAynn'nnrTG Cuts at: 518 1166 2595 3135 4070 4429 4588 518 Size: 648 1429 540 935 359 159 2042 Fragments arranged by size: 2042 1429 935 648 540 359 159 MspA1I CmG'CkG Cuts at: 1606 3528 4205 5353 5598 1606 Size: 1922 677 1148 245 2120 Fragments arranged by size: 2120 1922 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5560 NdeI CA'TA_TG Cuts at: 387 387 Size: 6112 NheI G'CTAG_C Cuts at: 1052 2746 1052 Size: 1694 4418 NotI GC'GGCC_GC Cuts at: 3559 3559 Size: 6112 NspI r_CATG'y Cuts at: 1132 1229 2914 3470 5015 1132 Size: 97 1685 556 1545 2229 Fragments arranged by size: 2229 1685 1545 556 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6112 PciI A'CATG_T Cuts at: 5011 5011 Size: 6112 PflMI CCAn_nnn'nTGG Cuts at: 1108 2576 1108 Size: 1468 4644 PfoI T'CCnGG_A Cuts at: 3262 3262 Size: 6112 PmeI GTTT'AAAC Cuts at: 3416 3416 Size: 6112 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4181 4213 5694 5726 4181 Size: 32 1481 32 4567 Fragments arranged by size: 4567 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6112 PsiI TTA'TAA Cuts at: 3682 3682 Size: 6112 PstI C_TGCA'G Cuts at: 830 3404 3464 830 Size: 2574 60 3478 Fragments arranged by size: 3478 2574 60 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6112 SacI G_AGCT'C Cuts at: 2946 2946 Size: 6112 SalI G'TCGA_C Cuts at: 1936 3454 1936 Size: 1518 4594 SbfI CC_TGCA'GG Cuts at: 3464 3464 Size: 6112 ScaI AGT'ACT Cuts at: 3047 4360 6072 3047 Size: 1313 1712 3087 Fragments arranged by size: 3087 1712 1313 SfcI C'TryA_G Cuts at: 826 1045 2350 3097 3400 3460 4595 5276 5467 Size: 219 1305 747 303 60 1135 681 191 Cuts at: 5467 826 Size: 1471 Fragments arranged by size: 1471 1305 1135 747 681 303 219 191 60 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6112 SmaI CCC'GGG Cuts at: 1872 3439 1872 Size: 1567 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4216 5117 5379 820 Size: 197 926 2273 901 262 1553 Fragments arranged by size: 2273 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3903 493 Size: 3410 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6112 SphI G_CATG'C Cuts at: 1132 3470 1132 Size: 2338 3774 SspI AAT'ATT Cuts at: 5 52 4036 5 Size: 47 3984 2081 Fragments arranged by size: 3984 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6112 StyI C'CwwG_G Cuts at: 513 1065 2454 3087 3551 513 Size: 552 1389 633 464 3074 Fragments arranged by size: 3074 1389 633 552 464 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4139 4156 4309 4494 4939 325 Size: 1255 2559 17 153 185 445 1498 Fragments arranged by size: 2559 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2631 3045 4358 Size: 274 80 33 51 1443 654 414 1313 Cuts at: 4358 6070 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1313 654 414 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2158 2190 3054 3086 6072 6104 2158 Size: 32 864 32 2986 32 2166 Fragments arranged by size: 2986 2166 864 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2102 1316 Size: 786 5326 XbaI T'CTAG_A Cuts at: 2074 3448 2074 Size: 1374 4738 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2780 2780 Size: 6112 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6112 XmaI C'CCGG_G Cuts at: 1870 3437 1870 Size: 1567 4545 XmnI GAAnn'nnTTC Cuts at: 2414 4241 2414 Size: 1827 4285 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3920 276 Size: 53 83 186 936 2386 2468 Fragments arranged by size: 2468 2386 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI