Restriction map for
pFN21AB5554
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AlwNI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I CelII Cfr10I ClaI
CpoI DrdI EagI EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
EcoT14I FauI FbaI FseI FspI
HaeII HapII HgaI Hin1I HpaI
HpaII KasI KpnI Ksp632I KspI
MfeI MflI MluI MluNI MroI
MscI MslI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccI 34 GTAGAC GT^[AC][GT]AC
AciI 840 GCGG CCGC(-3/-1)
AcsI 305 GAATTC [GA]^AATT[CT]
AfaI 472 GTAC GT^AC
AflIII 621 ACATGT A^C[GA][CT]GT
Alw44I 224 GTGCAC G^TGCAC
AlwI 357 GGATC GGATC(4/5)
AocI 886 CCTGAGG CC^T[ACGT]AGG
ApaLI 224 GTGCAC G^TGCAC
ApoI 305 GAATTC [GA]^AATT[CT]
BanI 846 GGCACC G^G[CT][GA]CC
BbvI 679 GCAGC GCAGC(8/12)
BciVI 147 GTATCC GTATCC(6/5)
BfaI 418 CTAG C^TAG
BpmI 434 CTCCAG CTGGAG(16/14)
BsaAI 197 TACGTG [CT]AC^GT[GA]
BsaBI 124 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 212 CCGTGG C^C[ACGT][ACGT]GG
BsgI 497 GTGCAG GTGCAG(16/14)
BsmAI 3 GTCTC GTCTC(1/5)
BspLU11I 621 ACATGT A^CATGT
BssKI 423 CCAGG ^CC[ACGT]GG
BstNI 423 CCAGG CC^[AT]GG
Bsu36I 886 CCTGAGG CC^T[ACGT]AGG
Cac8I 810 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 520 GCGC GCG^C
DraII 577 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 523 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 212 CCGTGG C^C[GA][CT]GG
EaeI 209 TGGCCG [CT]^GGCC[GA]
Eco81I 886 CCTGAGG CC^T[ACGT]AGG
EcoO109I 577 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 305 GAATTC G^AATTC
EcoRII 423 CCAGG ^CC[AT]GG
EcoT22I 601 ATGCAT ATGCA^T
Fnu4HI 679 GCAGC GC^[ACGT]GC
HhaI 520 GCGC GCG^C
HinP1I 520 GCGC G^CGC
HincII 774 GTCAAC GT[CT]^[GA]AC
HindII 774 GTCAAC GT[CT]^[GA]AC
HindIII 101 AAGCTT A^AGCTT
HphI 487 GGTGA GGTGA(8/7)
ItaI 679 GCAGC GC^[ACGT]GC
MaeI 418 CTAG C^TAG
MaeII 198 ACGT A^CGT
MamI 124 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvaI 423 CCAGG CC^[AT]GG
NsiI 601 ATGCAT ATGCA^T
NspI 621 ACATGT [GA]CATG^[CT]
PstI 677 CTGCAG CTGCA^G
RsaI 472 GTAC GT^AC
ScaI 471 AGTACT AGT^ACT
ScrFI 423 CCAGG CC^[ACGT]GG
TaqI 20 TCGA T^CGA
TfiI 333 GATTC G^A[AT]TC
TseI 679 GCAGC G^C[AT]GC
Tsp45I 572 GTGAC ^GT[GC]AC
TspRI 615 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 20 TCGA T^CGA
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# TWO CUT ENZYMES ( 19 enzymes) #
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AhdI 728 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
766 GACATACTGTC
AspEI 728 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
766 GACATACTGTC
AvaII 465 GGACC G^G[AT]CC
873 GGACC
BanII 154 GAGCTC G[GA]GC[CT]^C
507 GAGCTC
BbsI 22 GAAGAC GAAGAC(2/6)
692 GTCTTC
BcgI 593 CGATTACCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
327 GCAAGGGATTCG
BpuAI 22 GAAGAC GAAGAC(2/6)
692 GTCTTC
BsmFI 464 GGGAC GGGAC(10/14)
784 GGGAC
BstAPI 70 GCAAACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
522 GCACAAGGTGC
DpnII 358 GATC ^GATC
480 GATC
DraI 180 TTTAAA TTT^AAA
637 TTTAAA
Eam1105I 728 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
766 GACATACTGTC
HaeIII 210 GGCC GG^CC
578 GGCC
MboI 358 GATC ^GATC
480 GATC
NdeII 358 GATC ^GATC
480 GATC
PleI 377 GACTC GAGTC(4/5)
751 GACTC
SacI 154 GAGCTC GAGCT^C
507 GAGCTC
Sau3AI 358 GATC ^GATC
480 GATC
SfcI 677 CTGCAG C^T[GA][CT]AG
788 CTGTAG
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI AspHI BmyI BsiHKAI BsiYI
BslI Bsp1286I BsrI BstF5I Cfr13I
DdeI FokI HinfI MaeIII MboII
MnlI MseI MwoI NlaIII NlaIV
Sau96I Tru9I Tsp509I