Restriction map for
pFN21AB5484
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I ApaLI AscI
Asp700I Asp718I AvrII BalI BbsI
BcgI BciVI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraI DraIII
EcoRI EcoRV EcoT22I FseI HincII
HindII HindIII HpaI KpnI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SspBI SspI SwaI XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 430 GAATTT [GA]^AATT[CT]
AcyI 182 GGCGCC G[GA]^CG[CT]C
AflIII 315 ACGTGT A^C[GA][CT]GT
AhdI 1006 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 4 GGGCCC GGGCC^C
ApoI 430 GAATTT [GA]^AATT[CT]
AseI 973 ATTAAT AT^TAAT
AsnI 973 ATTAAT AT^TAAT
AspEI 1006 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 245 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1311 GGATCC G^GATCC
BbeI 182 GGCGCC GGCGC^C
BbrPI 314 CACGTG CAC^GTG
BbvCI 718 GCTGAGG CCTCAGC(-5/-2)
BclI 1757 TGATCA T^GATCA
BsaAI 314 CACGTG [CT]AC^GT[GA]
BsaHI 182 GGCGCC G[GA]^CG[CT]C
BsgI 562 CTGCAC GTGCAG(16/14)
BsmAI 251 GTCTC GTCTC(1/5)
BsmI 1650 GAATGC GAATGC(1/-1)
BssSI 919 CACGAG CACGAG(-5/-1)
BstEII 670 GGTGACC G^GT[ACGT]ACC
BstPI 670 GGTGACC G^GT[ACGT]ACC
DrdI 1722 GACAGGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1006 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1416 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 670 GGTGACC G^GT[ACGT]ACC
FbaI 1757 TGATCA T^GATCA
Hin1I 182 GGCGCC G[GA]^CG[CT]C
KasI 182 GGCGCC G^GCGCC
MaeII 315 ACGT A^CGT
MseI 974 TTAA T^TAA
NarI 182 GGCGCC GG^CGCC
PflFI 245 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1077 GACTC GAGTC(4/5)
PmaCI 314 CACGTG CAC^GTG
PmlI 314 CACGTG CAC^GTG
PshBI 973 ATTAAT AT^TAAT
SbfI 1194 CCTGCAGG CCTGCA^GG
ScaI 278 AGTACT AGT^ACT
SexAI 398 ACCAGGT A^CC[AT]GGT
SfoI 182 GGCGCC GGC^GCC
Sse8387I 1194 CCTGCAGG CCTGCA^GG
StuI 536 AGGCCT AGG^CCT
TaqI 243 TCGA T^CGA
Tru9I 974 TTAA T^TAA
Tth111I 245 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 243 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AfaI 279 GTAC GT^AC
294 GTAC
AocI 930 CCTGAGG CC^T[ACGT]AGG
1210 CCTCAGG
Aor51HI 236 AGCGCT AGC^GCT
350 AGCGCT
AspHI 916 GAGCAC G[AT]GC[AT]^C
1054 GAGCAC
AviII 206 TGCGCA TGC^GCA
1808 TGCGCA
BanII 4 GGGCCC G[GA]GC[CT]^C
129 GAGCCC
BsiEI 140 CGGCCG CG[GA][CT]^CG
213 CGGCCG
BsiHKAI 916 GAGCAC G[AT]GC[AT]^C
1054 GAGCAC
BsrBI 372 GAGCGG CCGCTC(-3/-3)
1766 GAGCGG
BssHII 93 GCGCGC G^CGCGC
95 GCGCGC
BstAPI 264 GCAGCGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1275 GCACACGCTGC
BstYI 1203 AGATCC [GA]^GATC[CT]
1311 GGATCC
Bsu36I 930 CCTGAGG CC^T[ACGT]AGG
1210 CCTCAGG
EagI 140 CGGCCG C^GGCCG
213 CGGCCG
EclXI 140 CGGCCG C^GGCCG
213 CGGCCG
Eco47III 236 AGCGCT AGC^GCT
350 AGCGCT
Eco52I 140 CGGCCG C^GGCCG
213 CGGCCG
Eco81I 930 CCTGAGG CC^T[ACGT]AGG
1210 CCTCAGG
FspI 206 TGCGCA TGC^GCA
1808 TGCGCA
MaeIII 671 GTGAC ^GT[ACGT]AC
850 GTCAC
MflI 1203 AGATCC [GA]^GATC[CT]
1311 GGATCC
PflMI 1293 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1839 CCATGGCGTGG
PvuII 378 CAGCTG CAG^CTG
1715 CAGCTG
RsaI 279 GTAC GT^AC
294 GTAC
SphI 575 GCATGC GCATG^C
896 GCATGC
Tsp45I 671 GTGAC ^GT[GC]AC
850 GTCAC
Tsp509I 431 AATT ^AATT
1481 AATT
Van91I 1293 CCAGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1839 CCATGGCGTGG
XhoII 1203 AGATCC [GA]^GATC[CT]
1311 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AvaI AvaII BanI BbvI BcnI
BglI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PpuMI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StyI TfiI
TseI TspRI XcmI XmaCI XmaI