(Circular) (Six-base) MAPSORT of: pFN21AB5484.seq Check: 3987 from: 1 to: 6655 pFN21AB5484 6655 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3085 3085 Size: 6655 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4465 278 Size: 53 83 186 936 2929 2468 Fragments arranged by size: 2929 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3998 1937 Size: 2061 4594 Acc65I G'GTAC_C Cuts at: 3976 3976 Size: 6655 AclI AA'CG_TT Cuts at: 1563 4782 5155 1563 Size: 3219 373 3063 Fragments arranged by size: 3219 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2484 2814 4718 6102 6370 1632 Size: 852 330 1904 1384 268 1917 Fragments arranged by size: 1917 1904 1384 852 330 268 AfeI AGC'GCT Cuts at: 2243 2357 2243 Size: 114 6541 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6458 AflIII A'CryG_T Cuts at: 1203 2320 5554 6531 1203 Size: 1117 3234 977 1327 Fragments arranged by size: 3234 1327 1117 977 AgeI A'CCGG_T Cuts at: 5486 5486 Size: 6655 AhdI GACnn_n'nnGTC Cuts at: 3016 5384 3016 Size: 2368 4287 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6655 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6655 AlwNI CAG_nnn'CTG Cuts at: 3052 3085 3118 3394 3433 4398 5970 3052 Size: 33 33 276 39 965 1572 3737 Fragments arranged by size: 3737 1572 965 276 39 33 33 ApaI G_GGCC'C Cuts at: 2013 2013 Size: 6655 ApaLI G'TGCA_C Cuts at: 4712 5868 4712 Size: 1156 5499 ApoI r'AATT_y Cuts at: 1493 2435 3964 4190 4342 6451 1493 Size: 942 1529 226 152 2109 1697 Fragments arranged by size: 2109 1697 1529 942 226 152 AseI AT'TA_AT Cuts at: 160 1032 2979 5209 160 Size: 872 1947 2230 1606 Fragments arranged by size: 2230 1947 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6655 AvaI C'yCGr_G Cuts at: 1870 1943 2047 2155 2575 2701 2866 3320 3980 Size: 73 104 108 420 126 165 454 660 Cuts at: 3980 1870 Size: 4545 Fragments arranged by size: 4545 660 454 420 165 126 108 104 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2282 2315 2282 Size: 33 6622 BamHI G'GATC_C Cuts at: 3316 3985 4352 3316 Size: 669 367 5619 Fragments arranged by size: 5619 669 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2187 2458 3715 3976 5431 Size: 325 217 627 400 271 1257 261 1455 Cuts at: 5431 618 Size: 1842 Fragments arranged by size: 1842 1455 1257 627 400 325 271 261 217 BanII G_rGCy'C Cuts at: 1391 2013 2138 3974 1391 Size: 622 125 1836 4072 Fragments arranged by size: 4072 1836 622 125 BbeI G_GCGC'C Cuts at: 2191 2191 Size: 6655 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5684 BbvCI CC'TCA_GC Cuts at: 2724 2724 Size: 6655 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4846 4880 1903 Size: 34 2909 34 3678 Fragments arranged by size: 3678 2909 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4547 5757 4547 Size: 1210 5445 BclI T'GATC_A Cuts at: 1548 3762 1548 Size: 2214 4441 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2049 2171 3203 Size: 107 122 71 631 605 377 122 1032 Cuts at: 3203 5266 136 Size: 2063 1525 Fragments arranged by size: 2063 1525 1032 631 605 377 122 122 107 71 BglII A'GATC_T Cuts at: 6650 6650 Size: 6655 BlpI GC'TnA_GC Cuts at: 4072 4072 Size: 6655 Bme1580I G_kGCm'C Cuts at: 1790 2013 2415 4716 5872 1790 Size: 223 402 2301 1156 2573 Fragments arranged by size: 2573 2301 1156 402 223 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6655 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3474 4386 5344 Size: 416 53 83 399 24 2057 912 958 Cuts at: 5344 442 Size: 1753 Fragments arranged by size: 2057 1753 958 912 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6655 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3803 3803 Size: 6655 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2300 2625 2975 3107 3525 3899 3962 5315 Size: 1061 325 350 132 418 374 63 1353 Cuts at: 5315 1239 Size: 2579 Fragments arranged by size: 2579 1353 1061 418 374 350 325 132 63 Bpu10I CC'TnA_GC Cuts at: 2724 3426 2724 Size: 702 5953 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4780 5645 5943 4780 Size: 865 298 5492 Fragments arranged by size: 5492 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5318 882 Size: 617 3819 2219 Fragments arranged by size: 3819 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2321 4446 493 Size: 711 1117 2125 2702 Fragments arranged by size: 2702 2125 1117 711 BsaBI GATnn'nnATC Cuts at: 6649 6649 Size: 6655 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2188 4462 4844 5479 Size: 53 83 186 936 655 2274 382 635 Cuts at: 5479 275 Size: 1451 Fragments arranged by size: 2274 1451 936 655 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5088 5486 5760 5907 6318 5088 Size: 398 274 147 411 5425 Fragments arranged by size: 5425 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2241 2271 2345 2375 2612 Size: 30 165 30 838 30 74 30 237 Cuts at: 2612 2642 3083 3113 1178 Size: 30 441 30 4720 Fragments arranged by size: 4720 838 441 237 165 74 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2753 2972 3104 3389 3446 3896 1186 Size: 1567 219 132 285 57 450 3945 Fragments arranged by size: 3945 1567 450 285 219 132 57 BseYI C'CCAG_C Cuts at: 2223 3722 5858 6354 2223 Size: 1499 2136 496 2524 Fragments arranged by size: 2524 2136 1499 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2552 1163 Size: 573 816 5266 Fragments arranged by size: 5266 816 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2148 2221 4105 4866 5894 1540 Size: 275 186 147 73 1884 761 1028 2301 Fragments arranged by size: 2301 1884 1028 761 275 186 147 73 BsiHKAI G_wGCw'C Cuts at: 2925 3063 4716 4801 5872 2925 Size: 138 1653 85 1071 3708 Fragments arranged by size: 3708 1653 1071 138 85 BsmI GAATG_Cn' Cuts at: 3661 4166 4259 3661 Size: 505 93 6057 Fragments arranged by size: 6057 505 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2138 2415 2925 3063 3974 4716 Size: 399 223 125 277 510 138 911 742 Cuts at: 4716 4801 5872 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 911 742 510 399 277 223 138 125 85 BspEI T'CCGG_A Cuts at: 6318 6318 Size: 6655 BspHI T'CATG_A Cuts at: 4544 6274 4544 Size: 1730 4925 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2217 3036 3085 3261 4010 844 Size: 1373 819 49 176 749 3489 Fragments arranged by size: 3489 1373 819 749 176 49 BsrBI CCG'CTC Cuts at: 1394 1823 2379 3773 4542 1394 Size: 429 556 1394 769 3507 Fragments arranged by size: 3507 1394 769 556 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5150 5324 65 Size: 1183 3902 174 1396 Fragments arranged by size: 3902 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2126 2216 2472 5299 5486 1700 Size: 110 316 90 256 2827 187 2869 Fragments arranged by size: 2869 2827 316 256 187 110 90 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6655 BssHII G'CGCG_C Cuts at: 1925 2098 2100 1925 Size: 173 2 6480 Fragments arranged by size: 6480 173 2 BssSI C'ACGA_G Cuts at: 2924 4715 5727 2924 Size: 1791 1012 3852 Fragments arranged by size: 3852 1791 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2275 3286 794 Size: 1481 1011 4163 Fragments arranged by size: 4163 1481 1011 BstBI TT'CG_AA Cuts at: 5459 5459 Size: 6655 BstEII G'GTnAC_C Cuts at: 1184 2675 1184 Size: 1491 5164 BstXI CCAn_nnnn'nTGG Cuts at: 2174 2350 3487 2174 Size: 176 1137 5342 Fragments arranged by size: 5342 1137 176 BstYI r'GATC_y Cuts at: 1972 3208 3316 3985 4352 4737 4754 6206 6292 Size: 1236 108 669 367 385 17 1452 86 Cuts at: 6292 6304 6650 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1236 669 385 367 346 108 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2936 3216 2936 Size: 280 6375 BtgI C'CryG_G Cuts at: 513 1065 2004 2349 2611 3003 3312 3844 513 Size: 552 939 345 262 392 309 532 3324 Fragments arranged by size: 3324 939 552 532 392 345 309 262 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3130 487 Size: 683 56 1904 4012 Fragments arranged by size: 4012 1904 683 56 BtsI GCAGTG_nn' Cuts at: 2847 4171 4965 4985 2847 Size: 1324 794 20 4517 Fragments arranged by size: 4517 1324 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6655 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6620 DraI TTT'AAA Cuts at: 3959 3959 Size: 6655 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3733 5662 809 Size: 507 2417 1929 1802 Fragments arranged by size: 2417 1929 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2124 2145 2218 Size: 54 1425 50 275 18 294 21 73 Cuts at: 2218 3306 4102 4991 6620 8 Size: 1088 796 889 1629 43 Fragments arranged by size: 1629 1425 1088 889 796 294 275 73 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 2145 2218 4102 1537 Size: 275 333 73 1884 4090 Fragments arranged by size: 4090 1884 333 275 73 EarI CTCTTCn'nnn_ Cuts at: 1353 2730 2790 2796 4585 1353 Size: 1377 60 6 1789 3423 Fragments arranged by size: 3423 1789 1377 60 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3201 3236 3792 5242 5616 5762 Size: 1064 138 1612 35 556 1450 374 146 Cuts at: 5762 387 Size: 1280 Fragments arranged by size: 1612 1450 1280 1064 556 374 146 138 35 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2300 2484 2625 2814 2975 3107 3525 Size: 393 668 184 141 189 161 132 418 Cuts at: 3525 3899 3962 4718 5315 6102 6370 1239 Size: 374 63 756 597 787 268 1524 Fragments arranged by size: 1524 787 756 668 597 418 393 374 268 189 184 161 141 132 63 EcoNI CCTnn'n_nnAGG Cuts at: 3425 3425 Size: 6655 EcoO109I rG'GnC_Cy Cuts at: 2009 2152 2206 2263 2449 2557 2646 3049 3476 Size: 143 54 57 186 108 89 403 427 Cuts at: 3476 2009 Size: 5188 Fragments arranged by size: 5188 427 403 186 143 108 89 57 54 EcoRI G'AATT_C Cuts at: 3964 4342 3964 Size: 378 6277 FspI TGC'GCA Cuts at: 2213 3815 5161 2213 Size: 1602 1346 3707 Fragments arranged by size: 3707 1602 1346 HaeII r_GCGC'y Cuts at: 1678 2191 2245 2359 3118 3169 3250 5802 1678 Size: 513 54 114 759 51 81 2552 2531 Fragments arranged by size: 2552 2531 759 513 114 81 54 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2241 2273 2729 2761 3002 Size: 32 135 32 669 32 456 32 241 Cuts at: 3002 3034 3083 3115 3370 3402 3587 3619 3734 Size: 32 49 32 255 32 185 32 115 Cuts at: 3734 3766 5296 5328 5370 5402 1373 Size: 32 1530 32 42 32 2626 Fragments arranged by size: 2626 1530 669 456 255 241 185 135 115 49 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3999 4245 669 Size: 988 281 2061 246 3079 Fragments arranged by size: 3079 2061 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6655 HpaI GTT'AAC Cuts at: 4245 4245 Size: 6655 KasI G'GCGC_C Cuts at: 2187 2187 Size: 6655 KpnI G_GTAC'C Cuts at: 3980 3980 Size: 6655 MfeI C'AATT_G Cuts at: 4254 4254 Size: 6655 MluI A'CGCG_T Cuts at: 6531 6531 Size: 6655 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5769 5953 1305 Size: 4464 184 2007 Fragments arranged by size: 4464 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4833 Fragments arranged by size: 4833 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2348 3563 3915 4613 4972 5131 518 Size: 648 1182 1215 352 698 359 159 2042 Fragments arranged by size: 2042 1215 1182 698 648 359 352 159 MspA1I CmG'CkG Cuts at: 1606 2024 2062 2068 2223 2385 2421 3631 3722 Size: 418 38 6 155 162 36 1210 91 Cuts at: 3722 3907 4071 4748 5896 6141 1606 Size: 185 164 677 1148 245 2120 Fragments arranged by size: 2120 1210 1148 677 418 245 185 164 162 155 91 38 36 6 NarI GG'CG_CC Cuts at: 2188 2188 Size: 6655 NcoI C'CATG_G Cuts at: 513 1065 2004 2349 3003 3844 513 Size: 552 939 345 654 841 3324 Fragments arranged by size: 3324 939 841 654 552 345 NdeI CA'TA_TG Cuts at: 387 387 Size: 6655 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6655 NotI GC'GGCC_GC Cuts at: 4102 4102 Size: 6655 NspI r_CATG'y Cuts at: 1132 1229 2584 2905 3305 4013 5558 1132 Size: 97 1355 321 400 708 1545 2229 Fragments arranged by size: 2229 1545 1355 708 400 321 97 PasI CC'CwG_GG Cuts at: 1348 2554 3503 1348 Size: 1206 949 4500 Fragments arranged by size: 4500 1206 949 PciI A'CATG_T Cuts at: 5554 5554 Size: 6655 PflMI CCAn_nnn'nTGG Cuts at: 1108 3304 3850 1108 Size: 2196 546 3913 Fragments arranged by size: 3913 2196 546 PfoI T'CCnGG_A Cuts at: 2259 2372 3447 3939 2259 Size: 113 1075 492 4975 Fragments arranged by size: 4975 1075 492 113 PmeI GTTT'AAAC Cuts at: 3959 3959 Size: 6655 PmlI CAC'GTG Cuts at: 2321 2321 Size: 6655 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4724 4756 6237 6269 4724 Size: 32 1481 32 5110 Fragments arranged by size: 5110 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2206 2263 2646 3476 2206 Size: 57 383 830 5385 Fragments arranged by size: 5385 830 383 57 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6655 PsiI TTA'TAA Cuts at: 4225 4225 Size: 6655 PspOMI G'GGCC_C Cuts at: 2009 2009 Size: 6655 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3914 3946 3914 Size: 32 6623 PstI C_TGCA'G Cuts at: 830 2291 2313 2549 3081 3204 3537 3906 4007 Size: 1461 22 236 532 123 333 369 101 Cuts at: 4007 830 Size: 3478 Fragments arranged by size: 3478 1461 532 369 333 236 123 101 22 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6655 PvuII CAG'CTG Cuts at: 2385 3722 2385 Size: 1337 5318 SalI G'TCGA_C Cuts at: 1936 3997 1936 Size: 2061 4594 SanDI GG'GwC_CC Cuts at: 2263 2646 3476 2263 Size: 383 830 5442 Fragments arranged by size: 5442 830 383 SbfI CC_TGCA'GG Cuts at: 3204 4007 3204 Size: 803 5852 ScaI AGT'ACT Cuts at: 2285 4903 6615 2285 Size: 2618 1712 2325 Fragments arranged by size: 2618 2325 1712 SexAI A'CCwGG_T Cuts at: 2403 2403 Size: 6655 SfcI C'TryA_G Cuts at: 826 1045 2287 2309 2545 3077 3200 3533 3902 Size: 219 1242 22 236 532 123 333 369 Cuts at: 3902 4003 5138 5819 6010 826 Size: 101 1135 681 191 1471 Fragments arranged by size: 1471 1242 1135 681 532 369 333 236 219 191 123 101 22 SfoI GGC'GCC Cuts at: 2189 2189 Size: 6655 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6655 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6655 SmaI CCC'GGG Cuts at: 1872 2049 2157 2703 3982 1872 Size: 177 108 546 1279 4545 Fragments arranged by size: 4545 1279 546 177 108 SmlI C'TyrA_G Cuts at: 820 1017 1943 4759 5660 5922 820 Size: 197 926 2816 901 262 1553 Fragments arranged by size: 2816 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4446 493 Size: 3953 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6655 SphI G_CATG'C Cuts at: 1132 2584 2905 4013 1132 Size: 1452 321 1108 3774 Fragments arranged by size: 3774 1452 1108 321 SrfI GCCC'GGGC Cuts at: 2049 2049 Size: 6655 SspI AAT'ATT Cuts at: 5 52 4579 5 Size: 47 4527 2081 Fragments arranged by size: 4527 2081 47 StuI AGG'CCT Cuts at: 1517 2543 1517 Size: 1026 5629 StyI C'CwwG_G Cuts at: 513 1065 2004 2349 3003 3177 3549 3668 3844 Size: 552 939 345 654 174 372 119 176 Cuts at: 3844 4094 513 Size: 250 3074 Fragments arranged by size: 3074 939 654 552 372 345 250 176 174 119 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4682 4699 4852 5037 5482 325 Size: 1255 3102 17 153 185 445 1498 Fragments arranged by size: 3102 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2283 4901 6613 Size: 274 80 33 51 1443 306 2618 1712 Cuts at: 6613 96 Size: 138 Fragments arranged by size: 2618 1712 1443 306 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2345 2377 2612 2644 3053 3085 6615 6647 2345 Size: 32 235 32 409 32 3530 32 2353 Fragments arranged by size: 3530 2353 409 235 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2253 1316 Size: 937 5718 XbaI T'CTAG_A Cuts at: 3991 3991 Size: 6655 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2119 2881 2950 3874 2119 Size: 762 69 924 4900 Fragments arranged by size: 4900 924 762 69 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6655 XmaI C'CCGG_G Cuts at: 1870 2047 2155 2701 3980 1870 Size: 177 108 546 1279 4545 Fragments arranged by size: 4545 1279 546 177 108 XmnI GAAnn'nnTTC Cuts at: 4784 4784 Size: 6655 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4463 276 Size: 53 83 186 936 2929 2468 Fragments arranged by size: 2929 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI RsrII SacI SacII SapI SfiI SwaI