(Circular) (Six-base) MAPSORT of: pFN21AB5477.seq Check: 5682 from: 1 to: 5878 pFN21AB5477 5878 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3688 278 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3221 1937 Size: 1284 4594 Acc65I G'GTAC_C Cuts at: 3199 3199 Size: 5878 AclI AA'CG_TT Cuts at: 1563 2653 4005 4378 1563 Size: 1090 1352 373 3063 Fragments arranged by size: 3063 1352 1090 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2342 3120 3941 5325 5593 1632 Size: 710 778 821 1384 268 1917 Fragments arranged by size: 1917 1384 821 778 710 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5681 AflIII A'CryG_T Cuts at: 1203 3063 4777 5754 1203 Size: 1860 1714 977 1327 Fragments arranged by size: 1860 1714 1327 977 AgeI A'CCGG_T Cuts at: 4709 4709 Size: 5878 AhdI GACnn_n'nnGTC Cuts at: 2454 4607 2454 Size: 2153 3725 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5878 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5878 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3155 3187 3155 Size: 32 5846 AlwNI CAG_nnn'CTG Cuts at: 2320 3621 5193 2320 Size: 1301 1572 3005 Fragments arranged by size: 3005 1572 1301 ApaLI G'TGCA_C Cuts at: 3935 5091 3935 Size: 1156 4722 ApoI r'AATT_y Cuts at: 1493 3187 3413 3565 5674 1493 Size: 1694 226 152 2109 1697 Fragments arranged by size: 2109 1697 1694 226 152 AseI AT'TA_AT Cuts at: 160 1032 4432 160 Size: 872 3400 1606 Fragments arranged by size: 3400 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 AvaI C'yCGr_G Cuts at: 1870 1943 2412 2870 3203 1870 Size: 73 469 458 333 4545 Fragments arranged by size: 4545 469 458 333 73 BamHI G'GATC_C Cuts at: 2272 2407 2472 3208 3575 2272 Size: 135 65 736 367 4575 Fragments arranged by size: 4575 736 367 135 65 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2399 3005 3199 4654 618 Size: 325 217 627 612 606 194 1455 1842 Fragments arranged by size: 1842 1455 627 612 606 325 217 194 BanII G_rGCy'C Cuts at: 1391 2260 3096 3197 1391 Size: 869 836 101 4072 Fragments arranged by size: 4072 869 836 101 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2520 2736 2864 2963 928 Size: 971 621 216 128 99 3843 Fragments arranged by size: 3843 971 621 216 128 99 BbvCI CC'TCA_GC Cuts at: 2706 2706 Size: 5878 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4069 4103 1903 Size: 34 2132 34 3678 Fragments arranged by size: 3678 2132 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3770 4980 3770 Size: 1210 4668 BclI T'GATC_A Cuts at: 1548 1548 Size: 5878 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4489 136 Size: 107 122 71 631 605 337 2480 1525 Fragments arranged by size: 2480 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5873 5873 Size: 5878 BlpI GC'TnA_GC Cuts at: 2103 3295 2103 Size: 1192 4686 Bme1580I G_kGCm'C Cuts at: 1790 2404 2584 3939 5095 1790 Size: 614 180 1355 1156 2573 Fragments arranged by size: 2573 1355 1156 614 180 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5878 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3609 4567 442 Size: 416 53 83 399 24 2192 958 1753 Fragments arranged by size: 2192 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5878 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2408 2630 4538 1239 Size: 1169 222 1908 2579 Fragments arranged by size: 2579 1908 1169 222 Bpu10I CC'TnA_GC Cuts at: 2706 2905 2706 Size: 199 5679 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4003 4868 5166 4003 Size: 865 298 4715 Fragments arranged by size: 4715 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4541 882 Size: 617 3042 2219 Fragments arranged by size: 3042 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3669 493 Size: 711 2465 2702 Fragments arranged by size: 2702 2465 711 BsaBI GATnn'nnATC Cuts at: 5872 5872 Size: 5878 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3685 4067 4702 275 Size: 53 83 186 936 2152 382 635 1451 Fragments arranged by size: 2152 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2141 2207 4311 4709 4983 5130 5541 2141 Size: 66 2104 398 274 147 411 2478 Fragments arranged by size: 2478 2104 411 398 274 147 66 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2867 2897 3155 3185 1178 Size: 30 165 30 1464 30 258 30 3871 Fragments arranged by size: 3871 1464 258 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2723 2726 2783 2888 3163 1186 Size: 1537 3 57 105 275 3901 Fragments arranged by size: 3901 1537 275 105 57 3 BseYI C'CCAG_C Cuts at: 5081 5577 5081 Size: 496 5382 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5305 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3052 3328 4089 5117 1540 Size: 275 186 1051 276 761 1028 2301 Fragments arranged by size: 2301 1051 1028 761 276 275 186 BsiHKAI G_wGCw'C Cuts at: 3939 4024 5095 3939 Size: 85 1071 4722 Fragments arranged by size: 4722 1071 85 BsmI GAATG_Cn' Cuts at: 3389 3482 3389 Size: 93 5785 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2260 2404 2584 3096 3197 3939 4024 Size: 399 470 144 180 512 101 742 85 Cuts at: 4024 5095 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 512 470 399 180 144 101 85 BspEI T'CCGG_A Cuts at: 2207 5541 2207 Size: 3334 2544 BspHI T'CATG_A Cuts at: 3767 5497 3767 Size: 1730 4148 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3233 844 Size: 2389 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2904 3765 1394 Size: 429 1081 861 3507 Fragments arranged by size: 3507 1081 861 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4373 4547 65 Size: 1183 3125 174 1396 Fragments arranged by size: 3125 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4522 4709 1700 Size: 110 2712 187 2869 Fragments arranged by size: 2869 2712 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5878 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5878 BssSI C'ACGA_G Cuts at: 3938 4950 3938 Size: 1012 4866 BstAPI GCAn_nnn'nTGC Cuts at: 794 2982 2991 3000 794 Size: 2188 9 9 3672 Fragments arranged by size: 3672 2188 9 9 BstBI TT'CG_AA Cuts at: 4682 4682 Size: 5878 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5878 BstYI r'GATC_y Cuts at: 1972 2272 2407 2472 3208 3575 3960 3977 5429 Size: 300 135 65 736 367 385 17 1452 Cuts at: 5429 5515 5527 5873 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 736 385 367 346 300 135 86 65 17 12 Bsu36I CC'TnA_GG Cuts at: 2268 2268 Size: 5878 BtgI C'CryG_G Cuts at: 513 1065 2004 2064 513 Size: 552 939 60 4327 Fragments arranged by size: 4327 939 552 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5139 Fragments arranged by size: 5139 683 56 BtsI GCAGTG_nn' Cuts at: 2078 2584 2958 2978 3394 4188 4208 2078 Size: 506 374 20 416 794 20 3748 Fragments arranged by size: 3748 794 506 416 374 20 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5878 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5843 DraI TTT'AAA Cuts at: 3182 3182 Size: 5878 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2190 4885 809 Size: 507 874 2695 1802 Fragments arranged by size: 2695 1802 874 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2899 3325 4214 Size: 54 1425 50 275 18 1069 426 889 Cuts at: 4214 5843 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1069 889 426 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3325 1537 Size: 275 1513 4090 Fragments arranged by size: 4090 1513 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3038 3808 1353 Size: 1685 770 3423 Fragments arranged by size: 3423 1685 770 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2257 4465 4839 4985 387 Size: 1064 138 668 2208 374 146 1280 Fragments arranged by size: 2208 1280 1064 668 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2342 2408 2630 3120 3941 4538 5325 Size: 393 710 66 222 490 821 597 787 Cuts at: 5325 5593 1239 Size: 268 1524 Fragments arranged by size: 1524 821 787 710 597 490 393 268 222 66 EcoNI CCTnn'n_nnAGG Cuts at: 2324 2324 Size: 5878 EcoO109I rG'GnC_Cy Cuts at: 2789 2789 Size: 5878 EcoRI G'AATT_C Cuts at: 3187 3565 3187 Size: 378 5500 FspI TGC'GCA Cuts at: 2375 4384 2375 Size: 2009 3869 HaeII r_GCGC'y Cuts at: 1678 2239 5025 1678 Size: 561 2786 2531 Fragments arranged by size: 2786 2531 561 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2440 2472 2658 2690 2762 Size: 32 135 32 868 32 186 32 72 Cuts at: 2762 2794 2867 2899 4519 4551 4593 4625 1373 Size: 32 73 32 1620 32 42 32 2626 Fragments arranged by size: 2626 1620 868 186 135 73 72 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3222 3468 669 Size: 988 281 1284 246 3079 Fragments arranged by size: 3079 1284 988 281 246 HindIII A'AGCT_T Cuts at: 748 3147 748 Size: 2399 3479 HpaI GTT'AAC Cuts at: 3468 3468 Size: 5878 KpnI G_GTAC'C Cuts at: 3203 3203 Size: 5878 MfeI C'AATT_G Cuts at: 2158 3477 2158 Size: 1319 4559 MluI A'CGCG_T Cuts at: 5754 5754 Size: 5878 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3089 4992 5176 1305 Size: 1784 1903 184 2007 Fragments arranged by size: 2007 1903 1784 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4056 Fragments arranged by size: 4056 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2171 3836 4195 4354 518 Size: 648 1005 1665 359 159 2042 Fragments arranged by size: 2042 1665 1005 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2215 2967 3294 3971 5119 5364 1606 Size: 609 752 327 677 1148 245 2120 Fragments arranged by size: 2120 1148 752 677 609 327 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2064 513 Size: 552 939 60 4327 Fragments arranged by size: 4327 939 552 60 NdeI CA'TA_TG Cuts at: 387 387 Size: 5878 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5878 NotI GC'GGCC_GC Cuts at: 3325 3325 Size: 5878 NspI r_CATG'y Cuts at: 1132 1229 3067 3236 4781 1132 Size: 97 1838 169 1545 2229 Fragments arranged by size: 2229 1838 1545 169 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5878 PciI A'CATG_T Cuts at: 3063 4777 3063 Size: 1714 4164 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5878 PfoI T'CCnGG_A Cuts at: 2194 2493 2607 2194 Size: 299 114 5465 Fragments arranged by size: 5465 299 114 PmeI GTTT'AAAC Cuts at: 3182 3182 Size: 5878 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3155 3187 3947 3979 5460 5492 3155 Size: 32 760 32 1481 32 3541 Fragments arranged by size: 3541 1481 760 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2789 2789 Size: 5878 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5878 PsiI TTA'TAA Cuts at: 3448 3448 Size: 5878 PstI C_TGCA'G Cuts at: 830 3230 830 Size: 2400 3478 PvuI CG_AT'CG Cuts at: 2001 3052 2001 Size: 1051 4827 PvuII CAG'CTG Cuts at: 2215 2215 Size: 5878 SalI G'TCGA_C Cuts at: 1936 3220 1936 Size: 1284 4594 SapI GCTCTTCn'nnn_ Cuts at: 3038 3038 Size: 5878 SbfI CC_TGCA'GG Cuts at: 3230 3230 Size: 5878 ScaI AGT'ACT Cuts at: 2051 4126 5838 2051 Size: 2075 1712 2091 Fragments arranged by size: 2091 2075 1712 SfcI C'TryA_G Cuts at: 826 1045 3226 4361 5042 5233 826 Size: 219 2181 1135 681 191 1471 Fragments arranged by size: 2181 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5878 SmaI CCC'GGG Cuts at: 1872 3205 1872 Size: 1333 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3982 4883 5145 820 Size: 197 926 2039 901 262 1553 Fragments arranged by size: 2039 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3669 493 Size: 3176 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5878 SphI G_CATG'C Cuts at: 1132 3236 1132 Size: 2104 3774 SspI AAT'ATT Cuts at: 5 52 3802 5 Size: 47 3750 2081 Fragments arranged by size: 3750 2081 47 StuI AGG'CCT Cuts at: 1517 2673 1517 Size: 1156 4722 StyI C'CwwG_G Cuts at: 513 1065 2004 2038 2064 2559 3317 513 Size: 552 939 34 26 495 758 3074 Fragments arranged by size: 3074 939 758 552 495 34 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2758 3905 3922 4075 4260 4705 325 Size: 1255 1178 1147 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1178 1147 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2049 2217 3066 Size: 274 80 33 51 1443 72 168 849 Cuts at: 3066 4124 5836 96 Size: 1058 1712 138 Fragments arranged by size: 1712 1443 1058 849 274 168 138 80 72 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5838 5870 5838 Size: 32 5846 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5878 XbaI T'CTAG_A Cuts at: 3214 3214 Size: 5878 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5878 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5878 XmaI C'CCGG_G Cuts at: 1870 3203 1870 Size: 1333 4545 XmnI GAAnn'nnTTC Cuts at: 4007 4007 Size: 5878 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3686 276 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI