Restriction map for
pFN21AB5477
(Linear) (Six-base) MAPSORT of: KIBB5477.seq Check: 8470 from: 1 to: 1173
KIBB5477.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AclI AA'CG_TT
Cuts at: 0 648 1173
Size: 648 525
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 1115 1173
Size: 337 778 58
Fragments arranged by size:
778 337 58
AflIII A'CryG_T
Cuts at: 0 1058 1173
Size: 1058 115
AhdI GACnn_n'nnGTC
Cuts at: 0 449 1173
Size: 449 724
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1150 1173
Size: 1150 23
AlwNI CAG_nnn'CTG
Cuts at: 0 315 1173
Size: 315 858
AvaI C'yCGr_G
Cuts at: 0 407 865 1173
Size: 407 458 308
Fragments arranged by size:
458 407 308
BamHI G'GATC_C
Cuts at: 0 267 402 467 1173
Size: 267 135 65 706
Fragments arranged by size:
706 267 135 65
BanI G'GyrC_C
Cuts at: 0 394 1000 1173
Size: 394 606 173
Fragments arranged by size:
606 394 173
BanII G_rGCy'C
Cuts at: 0 255 1091 1173
Size: 255 836 82
Fragments arranged by size:
836 255 82
BbsI GAAGACnn'nnnn_
Cuts at: 0 515 731 859 958 1173
Size: 515 216 128 99 215
Fragments arranged by size:
515 216 215 128 99
BbvCI CC'TCA_GC
Cuts at: 0 701 1173
Size: 701 472
BlpI GC'TnA_GC
Cuts at: 0 98 1173
Size: 98 1075
Bme1580I G_kGCm'C
Cuts at: 0 399 579 1173
Size: 399 180 594
Fragments arranged by size:
594 399 180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 625 1173
Size: 403 222 548
Fragments arranged by size:
548 403 222
Bpu10I CC'TnA_GC
Cuts at: 0 701 900 1173
Size: 701 199 273
Fragments arranged by size:
701 273 199
BsaWI w'CCGG_w
Cuts at: 0 136 202 1173
Size: 136 66 971
Fragments arranged by size:
971 136 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 862 892 1150 1173
Size: 862 30 258 23
Fragments arranged by size:
862 258 30 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 718 721 778 883 1158 1173
Size: 718 3 57 105 275 15
Fragments arranged by size:
718 275 105 57 15 3
BsiEI CG_ry'CG
Cuts at: 0 1047 1173
Size: 1047 126
Bsp1286I G_dGCh'C
Cuts at: 0 255 399 579 1091 1173
Size: 255 144 180 512 82
Fragments arranged by size:
512 255 180 144 82
BspEI T'CCGG_A
Cuts at: 0 202 1173
Size: 202 971
BsrBI CCG'CTC
Cuts at: 0 899 1173
Size: 899 274
BstAPI GCAn_nnn'nTGC
Cuts at: 0 977 986 995 1173
Size: 977 9 9 178
Fragments arranged by size:
977 178 9 9
BstYI r'GATC_y
Cuts at: 0 267 402 467 1173
Size: 267 135 65 706
Fragments arranged by size:
706 267 135 65
Bsu36I CC'TnA_GG
Cuts at: 0 263 1173
Size: 263 910
BtgI C'CryG_G
Cuts at: 0 59 1173
Size: 59 1114
BtsI GCAGTG_nn'
Cuts at: 0 73 579 953 973 1173
Size: 73 506 374 20 200
Fragments arranged by size:
506 374 200 73 20
DrdI GACnn_nn'nnGTC
Cuts at: 0 185 1173
Size: 185 988
EaeI y'GGCC_r
Cuts at: 0 894 1173
Size: 894 279
EarI CTCTTCn'nnn_
Cuts at: 0 1033 1173
Size: 1033 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 252 1173
Size: 252 921
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 403 625 1115 1173
Size: 337 66 222 490 58
Fragments arranged by size:
490 337 222 66 58
EcoNI CCTnn'n_nnAGG
Cuts at: 0 319 1173
Size: 319 854
EcoO109I rG'GnC_Cy
Cuts at: 0 784 1173
Size: 784 389
FspI TGC'GCA
Cuts at: 0 370 1173
Size: 370 803
HaeII r_GCGC'y
Cuts at: 0 234 1173
Size: 234 939
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 435 467 653 685 757 789 862 894
Size: 435 32 186 32 72 32 73 32
Cuts at: 894 1173
Size: 279
Fragments arranged by size:
435 279 186 73 72 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 1142 1173
Size: 1142 31
MfeI C'AATT_G
Cuts at: 0 153 1173
Size: 153 1020
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1084 1173
Size: 1084 89
MslI CAynn'nnrTG
Cuts at: 0 166 1173
Size: 166 1007
MspA1I CmG'CkG
Cuts at: 0 210 962 1173
Size: 210 752 211
Fragments arranged by size:
752 211 210
NcoI C'CATG_G
Cuts at: 0 59 1173
Size: 59 1114
NspI r_CATG'y
Cuts at: 0 1062 1173
Size: 1062 111
PciI A'CATG_T
Cuts at: 0 1058 1173
Size: 1058 115
PfoI T'CCnGG_A
Cuts at: 0 189 488 602 1173
Size: 189 299 114 571
Fragments arranged by size:
571 299 189 114
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1150 1173
Size: 1150 23
PpuMI rG'GwC_Cy
Cuts at: 0 784 1173
Size: 784 389
PvuI CG_AT'CG
Cuts at: 0 1047 1173
Size: 1047 126
PvuII CAG'CTG
Cuts at: 0 210 1173
Size: 210 963
SapI GCTCTTCn'nnn_
Cuts at: 0 1033 1173
Size: 1033 140
ScaI AGT'ACT
Cuts at: 0 46 1173
Size: 46 1127
StuI AGG'CCT
Cuts at: 0 668 1173
Size: 668 505
StyI C'CwwG_G
Cuts at: 0 33 59 554 1173
Size: 33 26 495 619
Fragments arranged by size:
619 495 33 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 753 1173
Size: 753 420
TatI w'GTAC_w
Cuts at: 0 44 212 1061 1173
Size: 44 168 849 112
Fragments arranged by size:
849 168 112 44
Enzymes that do cut:
AclI AcuI AflIII AhdI AloI AlwNI AvaI BamHI
BanI BanII BbsI BbvCI BlpI Bme1580I BpmI Bpu10I
BsaWI BsaXI BseRI BsiEI Bsp1286I BspEI BsrBI BstAPI
BstYI Bsu36I BtgI BtsI DrdI EaeI EarI EciI
Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HindIII MfeI
MmeI MslI MspA1I NcoI NspI PciI PfoI PpiI
PpuMI PvuI PvuII SapI ScaI StuI StyI TaqII
TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AleI
AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BcgI BciVI BclI BfrBI BglI BglII
BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BseYI BsgI BsiHKAI BsiWI BsmI BsmBI BspHI
BspMI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII EagI
EcoICRI EcoRI EcoRV FalI FseI FspAI HincII HpaI
KasI KpnI MluI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PshAI PsiI PspOMI PspXI PsrI PstI RsrII
SacI SacII SalI SanDI SbfI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI