(Circular) (Six-base) MAPSORT of: pFN21AB5457.seq Check: 9491 from: 1 to: 6154 pFN21AB5457 6154 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2760 2760 Size: 6154 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3964 278 Size: 53 83 186 936 2428 2468 Fragments arranged by size: 2468 2428 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3497 1937 Size: 1560 4594 Acc65I G'GTAC_C Cuts at: 2269 3475 2269 Size: 1206 4948 AclI AA'CG_TT Cuts at: 1563 4281 4654 1563 Size: 2718 373 3063 Fragments arranged by size: 3063 2718 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2219 2903 2936 3146 4217 5601 5869 1632 Size: 587 684 33 210 1071 1384 268 1917 Fragments arranged by size: 1917 1384 1071 684 587 268 210 33 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5957 AflIII A'CryG_T Cuts at: 1203 5053 6030 1203 Size: 3850 977 1327 Fragments arranged by size: 3850 1327 977 AgeI A'CCGG_T Cuts at: 4985 4985 Size: 6154 AhdI GACnn_n'nnGTC Cuts at: 4883 4883 Size: 6154 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6154 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2768 3278 1184 Size: 1584 510 4060 Fragments arranged by size: 4060 1584 510 AlwNI CAG_nnn'CTG Cuts at: 2176 3897 5469 2176 Size: 1721 1572 2861 Fragments arranged by size: 2861 1721 1572 ApaI G_GGCC'C Cuts at: 2410 2410 Size: 6154 ApaLI G'TGCA_C Cuts at: 2561 4211 5367 2561 Size: 1650 1156 3348 Fragments arranged by size: 3348 1650 1156 ApoI r'AATT_y Cuts at: 1493 2311 2364 2895 3463 3689 3841 5950 1493 Size: 818 53 531 568 226 152 2109 1697 Fragments arranged by size: 2109 1697 818 568 531 226 152 53 AseI AT'TA_AT Cuts at: 160 1032 4708 160 Size: 872 3676 1606 Fragments arranged by size: 3676 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6154 AvaI C'yCGr_G Cuts at: 1870 1943 3479 1870 Size: 73 1536 4545 Fragments arranged by size: 4545 1536 73 AvrII C'CTAG_G Cuts at: 3121 3121 Size: 6154 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2261 2294 2261 Size: 33 6121 BamHI G'GATC_C Cuts at: 3484 3851 3484 Size: 367 5787 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2165 2269 2340 3425 3475 Size: 325 217 627 378 104 71 1085 50 Cuts at: 3475 4930 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1085 627 378 325 217 104 71 50 BanII G_rGCy'C Cuts at: 1391 2410 3361 3473 1391 Size: 1019 951 112 4072 Fragments arranged by size: 4072 1019 951 112 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5183 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4345 4379 1903 Size: 34 2408 34 3678 Fragments arranged by size: 3678 2408 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2275 4046 5256 2275 Size: 1771 1210 3173 Fragments arranged by size: 3173 1771 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6154 BfrBI ATG'CAT Cuts at: 3140 3140 Size: 6154 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2141 3423 4765 Size: 107 122 71 631 605 469 1282 1342 Cuts at: 4765 136 Size: 1525 Fragments arranged by size: 1525 1342 1282 631 605 469 122 107 71 BglII A'GATC_T Cuts at: 6149 6149 Size: 6154 BlpI GC'TnA_GC Cuts at: 3339 3571 3339 Size: 232 5922 Bme1580I G_kGCm'C Cuts at: 1790 2170 2410 2565 2792 4215 5371 1790 Size: 380 240 155 227 1423 1156 2573 Fragments arranged by size: 2573 1423 1156 380 240 227 155 BmgBI CAC'GTC Cuts at: 1318 2626 1318 Size: 1308 4846 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2164 2530 3249 Size: 416 53 83 399 24 747 366 719 Cuts at: 3249 3885 4843 442 Size: 636 958 1753 Fragments arranged by size: 1753 958 747 719 636 416 399 366 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2907 1056 Size: 1851 4303 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2206 2707 2206 Size: 501 5653 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2523 2984 4814 1239 Size: 1284 461 1830 2579 Fragments arranged by size: 2579 1830 1284 461 Bpu10I CC'TnA_GC Cuts at: 3029 3029 Size: 6154 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2336 2708 2997 4279 5144 5442 2336 Size: 372 289 1282 865 298 3048 Fragments arranged by size: 3048 1282 865 372 298 289 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2194 2340 4817 882 Size: 617 695 146 2477 2219 Fragments arranged by size: 2477 2219 695 617 146 BsaAI yAC'GTr Cuts at: 493 1204 3945 493 Size: 711 2741 2702 Fragments arranged by size: 2741 2702 711 BsaBI GATnn'nnATC Cuts at: 2705 6148 2705 Size: 3443 2711 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3961 4343 4978 275 Size: 53 83 186 936 2428 382 635 1451 Fragments arranged by size: 2428 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4587 4985 5259 5406 5817 4587 Size: 398 274 147 411 4924 Fragments arranged by size: 4924 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2841 2871 1178 Size: 30 165 30 1438 30 4461 Fragments arranged by size: 4461 1438 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6154 BseYI C'CCAG_C Cuts at: 2234 2629 5357 5853 2234 Size: 395 2728 496 2535 Fragments arranged by size: 2728 2535 496 395 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5581 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3604 4365 5393 1540 Size: 275 186 1603 761 1028 2301 Fragments arranged by size: 2301 1603 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2565 4215 4300 5371 2565 Size: 1650 85 1071 3348 Fragments arranged by size: 3348 1650 1071 85 BsmI GAATG_Cn' Cuts at: 3255 3665 3758 3255 Size: 410 93 5651 Fragments arranged by size: 5651 410 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2170 2410 2565 2792 3361 3473 4215 Size: 399 380 240 155 227 569 112 742 Cuts at: 4215 4300 5371 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 569 399 380 240 227 155 112 85 BspEI T'CCGG_A Cuts at: 5817 5817 Size: 6154 BspHI T'CATG_A Cuts at: 4043 5773 4043 Size: 1730 4424 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2760 3509 844 Size: 1916 749 3489 Fragments arranged by size: 3489 1916 749 BsrBI CCG'CTC Cuts at: 1394 1823 2382 4041 1394 Size: 429 559 1659 3507 Fragments arranged by size: 3507 1659 559 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2481 4649 4823 65 Size: 1183 1233 2168 174 1396 Fragments arranged by size: 2168 1396 1233 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4798 4985 1700 Size: 110 2988 187 2869 Fragments arranged by size: 2988 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6154 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6154 BssSI C'ACGA_G Cuts at: 4214 5226 4214 Size: 1012 5142 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6154 BstBI TT'CG_AA Cuts at: 4958 4958 Size: 6154 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6154 BstXI CCAn_nnnn'nTGG Cuts at: 2161 2942 2161 Size: 781 5373 BstYI r'GATC_y Cuts at: 1972 3484 3851 4236 4253 5705 5791 5803 6149 Size: 1512 367 385 17 1452 86 12 346 Cuts at: 6149 1972 Size: 1977 Fragments arranged by size: 1977 1512 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2077 3418 513 Size: 552 939 73 1341 3249 Fragments arranged by size: 3249 1341 939 552 73 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2005 2406 3061 487 Size: 683 56 779 401 655 3580 Fragments arranged by size: 3580 779 683 655 401 56 BtsI GCAGTG_nn' Cuts at: 3276 3670 4464 4484 3276 Size: 394 794 20 4946 Fragments arranged by size: 4946 794 394 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6154 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3031 3052 3066 3087 529 Size: 35 2467 21 14 21 3596 Fragments arranged by size: 3596 2467 35 21 21 14 DraI TTT'AAA Cuts at: 3458 3458 Size: 6154 DraIII CAC_nnn'GTG Cuts at: 2551 2551 Size: 6154 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5161 809 Size: 507 3845 1802 Fragments arranged by size: 3845 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2133 3378 Size: 54 1425 50 275 18 207 96 1245 Cuts at: 3378 3601 4490 6119 8 Size: 223 889 1629 43 Fragments arranged by size: 1629 1425 1245 889 275 223 207 96 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3601 1537 Size: 275 1789 4090 Fragments arranged by size: 4090 1789 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4084 1353 Size: 2731 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2227 2803 4741 5115 5261 387 Size: 1064 138 638 576 1938 374 146 1280 Fragments arranged by size: 1938 1280 1064 638 576 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2219 2523 2903 2936 2984 3146 4217 Size: 393 587 304 380 33 48 162 1071 Cuts at: 4217 4814 5601 5869 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1071 787 597 587 393 380 304 268 162 48 33 EcoNI CCTnn'n_nnAGG Cuts at: 2228 3332 2228 Size: 1104 5050 EcoO109I rG'GnC_Cy Cuts at: 2406 2921 2406 Size: 515 5639 EcoRI G'AATT_C Cuts at: 2895 3463 3841 2895 Size: 568 378 5208 Fragments arranged by size: 5208 568 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2488 2488 Size: 6154 FspI TGC'GCA Cuts at: 4660 4660 Size: 6154 HaeII r_GCGC'y Cuts at: 1678 5301 1678 Size: 3623 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2025 2057 2841 2873 2939 Size: 32 135 32 453 32 784 32 66 Cuts at: 2939 2971 4795 4827 4869 4901 1373 Size: 32 1824 32 42 32 2626 Fragments arranged by size: 2626 1824 784 453 135 66 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3498 3744 669 Size: 988 281 1560 246 3079 Fragments arranged by size: 3079 1560 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6154 HpaI GTT'AAC Cuts at: 3744 3744 Size: 6154 KpnI G_GTAC'C Cuts at: 2273 3479 2273 Size: 1206 4948 MfeI C'AATT_G Cuts at: 3753 3753 Size: 6154 MluI A'CGCG_T Cuts at: 6030 6030 Size: 6154 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2259 5268 5452 1305 Size: 954 3009 184 2007 Fragments arranged by size: 3009 2007 954 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2135 3380 10 Size: 54 1425 343 303 1245 2784 Fragments arranged by size: 2784 1425 1245 343 303 54 MslI CAynn'nnrTG Cuts at: 518 1166 3260 3366 4112 4471 4630 518 Size: 648 2094 106 746 359 159 2042 Fragments arranged by size: 2094 2042 746 648 359 159 106 MspA1I CmG'CkG Cuts at: 1606 2593 3197 3343 3570 4247 5395 5640 1606 Size: 987 604 146 227 677 1148 245 2120 Fragments arranged by size: 2120 1148 987 677 604 245 227 146 NcoI C'CATG_G Cuts at: 513 1065 2004 3418 513 Size: 552 939 1414 3249 Fragments arranged by size: 3249 1414 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6154 NheI G'CTAG_C Cuts at: 1052 2903 1052 Size: 1851 4303 NotI GC'GGCC_GC Cuts at: 3601 3601 Size: 6154 NsiI A_TGCA'T Cuts at: 3142 3142 Size: 6154 NspI r_CATG'y Cuts at: 1132 1229 2590 3512 5057 1132 Size: 97 1361 922 1545 2229 Fragments arranged by size: 2229 1545 1361 922 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6154 PciI A'CATG_T Cuts at: 5053 5053 Size: 6154 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6154 PfoI T'CCnGG_A Cuts at: 2961 2961 Size: 6154 PmeI GTTT'AAAC Cuts at: 3458 3458 Size: 6154 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4223 4255 5736 5768 4223 Size: 32 1481 32 4609 Fragments arranged by size: 4609 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2921 2921 Size: 6154 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6154 PsiI TTA'TAA Cuts at: 3724 3724 Size: 6154 PspOMI G'GGCC_C Cuts at: 2406 2406 Size: 6154 PstI C_TGCA'G Cuts at: 830 3506 830 Size: 2676 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6154 PvuII CAG'CTG Cuts at: 2593 3197 2593 Size: 604 5550 SalI G'TCGA_C Cuts at: 1936 3496 1936 Size: 1560 4594 SanDI GG'GwC_CC Cuts at: 2921 2921 Size: 6154 SbfI CC_TGCA'GG Cuts at: 3506 3506 Size: 6154 ScaI AGT'ACT Cuts at: 4402 6114 4402 Size: 1712 4442 SfcI C'TryA_G Cuts at: 826 1045 3502 4637 5318 5509 826 Size: 219 2457 1135 681 191 1471 Fragments arranged by size: 2457 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6154 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6154 SmaI CCC'GGG Cuts at: 1872 3481 1872 Size: 1609 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2351 2723 3012 4258 5159 5421 Size: 197 926 408 372 289 1246 901 262 Cuts at: 5421 820 Size: 1553 Fragments arranged by size: 1553 1246 926 901 408 372 289 262 197 SnaBI TAC'GTA Cuts at: 493 3945 493 Size: 3452 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6154 SphI G_CATG'C Cuts at: 1132 3512 1132 Size: 2380 3774 SspI AAT'ATT Cuts at: 5 52 2719 4078 5 Size: 47 2667 1359 2081 Fragments arranged by size: 2667 2081 1359 47 StuI AGG'CCT Cuts at: 1517 2660 1517 Size: 1143 5011 StyI C'CwwG_G Cuts at: 513 1065 2004 2358 2476 3121 3418 3593 513 Size: 552 939 354 118 645 297 175 3074 Fragments arranged by size: 3074 939 645 552 354 297 175 118 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2153 2512 4181 4198 4351 4536 4981 Size: 1255 573 359 1669 17 153 185 445 Cuts at: 4981 325 Size: 1498 Fragments arranged by size: 1669 1498 1255 573 445 359 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4400 6112 96 Size: 274 80 33 51 1443 2423 1712 138 Fragments arranged by size: 2423 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2142 2174 6114 6146 2142 Size: 32 3940 32 2150 Fragments arranged by size: 3940 2150 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6154 XbaI T'CTAG_A Cuts at: 3490 3490 Size: 6154 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2738 2815 2738 Size: 77 6077 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6154 XmaI C'CCGG_G Cuts at: 1870 3479 1870 Size: 1609 4545 XmnI GAAnn'nnTTC Cuts at: 2957 4283 2957 Size: 1326 4828 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3962 276 Size: 53 83 186 936 2428 2468 Fragments arranged by size: 2468 2428 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BbeI BbvCI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI