(Circular) (Six-base) MAPSORT of: pFN21AB5420.seq Check: 5107 from: 1 to: 6007 pFN21AB5420 6007 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3817 278 Size: 53 83 186 936 2281 2468 Fragments arranged by size: 2468 2281 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3350 1937 Size: 1413 4594 Acc65I G'GTAC_C Cuts at: 3328 3328 Size: 6007 AclI AA'CG_TT Cuts at: 1563 4134 4507 1563 Size: 2571 373 3063 Fragments arranged by size: 3063 2571 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2080 2448 2521 2600 2705 2743 3050 3153 Size: 448 368 73 79 105 38 307 103 Cuts at: 3153 3271 4070 5454 5722 1632 Size: 118 799 1384 268 1917 Fragments arranged by size: 1917 1384 799 448 368 307 268 118 105 103 79 73 38 AfeI AGC'GCT Cuts at: 3212 3212 Size: 6007 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5810 AflIII A'CryG_T Cuts at: 1203 4906 5883 1203 Size: 3703 977 1327 Fragments arranged by size: 3703 1327 977 AgeI A'CCGG_T Cuts at: 4838 4838 Size: 6007 AhdI GACnn_n'nnGTC Cuts at: 2788 4736 2788 Size: 1948 4059 AleI CACnn'nnGTG Cuts at: 1166 2621 1166 Size: 1455 4552 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6007 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2993 3025 2993 Size: 32 5975 AlwNI CAG_nnn'CTG Cuts at: 3750 5322 3750 Size: 1572 4435 ApaLI G'TGCA_C Cuts at: 3169 4064 5220 3169 Size: 895 1156 3956 Fragments arranged by size: 3956 1156 895 ApoI r'AATT_y Cuts at: 1493 3316 3542 3694 5803 1493 Size: 1823 226 152 2109 1697 Fragments arranged by size: 2109 1823 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4561 160 Size: 872 3529 1606 Fragments arranged by size: 3529 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 AvaI C'yCGr_G Cuts at: 1870 1943 3332 1870 Size: 73 1389 4545 Fragments arranged by size: 4545 1389 73 BamHI G'GATC_C Cuts at: 3337 3704 3337 Size: 367 5640 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2369 3191 3328 4783 618 Size: 325 217 627 582 822 137 1455 1842 Fragments arranged by size: 1842 1455 822 627 582 325 217 137 BanII G_rGCy'C Cuts at: 1391 2151 3326 1391 Size: 760 1175 4072 Fragments arranged by size: 4072 1175 760 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2693 928 Size: 971 794 4242 Fragments arranged by size: 4242 971 794 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4198 4232 1903 Size: 34 2261 34 3678 Fragments arranged by size: 3678 2261 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3899 5109 3899 Size: 1210 4797 BclI T'GATC_A Cuts at: 1548 1548 Size: 6007 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4618 136 Size: 107 122 71 631 605 2946 1525 Fragments arranged by size: 2946 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2034 6002 2034 Size: 3968 2039 BlpI GC'TnA_GC Cuts at: 3424 3424 Size: 6007 Bme1580I G_kGCm'C Cuts at: 1790 2112 3173 3196 4068 5224 1790 Size: 322 1061 23 872 1156 2573 Fragments arranged by size: 2573 1156 1061 872 322 23 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6007 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2106 2513 2861 Size: 416 53 83 399 24 689 407 348 Cuts at: 2861 2914 3183 3738 4696 442 Size: 53 269 555 958 1753 Fragments arranged by size: 1753 958 689 555 416 407 399 348 269 83 53 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6007 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2678 2696 4667 1239 Size: 1439 18 1971 2579 Fragments arranged by size: 2579 1971 1439 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2000 4132 4997 5295 2000 Size: 2132 865 298 2712 Fragments arranged by size: 2712 2132 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2096 4670 882 Size: 617 597 2574 2219 Fragments arranged by size: 2574 2219 617 597 BsaAI yAC'GTr Cuts at: 493 1204 3798 493 Size: 711 2594 2702 Fragments arranged by size: 2702 2594 711 BsaBI GATnn'nnATC Cuts at: 6001 6001 Size: 6007 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3102 3814 4196 4831 Size: 53 83 186 936 1569 712 382 635 Cuts at: 4831 275 Size: 1451 Fragments arranged by size: 1569 1451 936 712 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4440 4838 5112 5259 5670 4440 Size: 398 274 147 411 4777 Fragments arranged by size: 4777 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2550 2580 1178 Size: 30 165 30 1147 30 4605 Fragments arranged by size: 4605 1147 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3224 3263 1186 Size: 2038 39 3930 Fragments arranged by size: 3930 2038 39 BseYI C'CCAG_C Cuts at: 2804 3253 5210 5706 2804 Size: 449 1957 496 3105 Fragments arranged by size: 3105 1957 496 449 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2547 2955 1163 Size: 573 811 408 4215 Fragments arranged by size: 4215 811 573 408 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3457 4218 5246 1540 Size: 275 186 1456 761 1028 2301 Fragments arranged by size: 2301 1456 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2151 2208 3173 4068 4153 5224 2151 Size: 57 965 895 85 1071 2934 Fragments arranged by size: 2934 1071 965 895 85 57 BsmI GAATG_Cn' Cuts at: 3518 3611 3518 Size: 93 5914 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2112 2151 2208 3173 3196 3326 4068 Size: 399 322 39 57 965 23 130 742 Cuts at: 4068 4153 5224 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 965 742 399 322 130 85 57 39 23 BspEI T'CCGG_A Cuts at: 5670 5670 Size: 6007 BspHI T'CATG_A Cuts at: 2508 3896 5626 2508 Size: 1388 1730 2889 Fragments arranged by size: 2889 1730 1388 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2799 3362 844 Size: 1955 563 3489 Fragments arranged by size: 3489 1955 563 BsrBI CCG'CTC Cuts at: 1394 1823 3894 1394 Size: 429 2071 3507 Fragments arranged by size: 3507 2071 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4502 4676 65 Size: 1183 3254 174 1396 Fragments arranged by size: 3254 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3275 4651 4838 1700 Size: 110 1465 1376 187 2869 Fragments arranged by size: 2869 1465 1376 187 110 BsrGI T'GTAC_A Cuts at: 96 2841 96 Size: 2745 3262 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6007 BssSI C'ACGA_G Cuts at: 4067 5079 4067 Size: 1012 4995 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6007 BstBI TT'CG_AA Cuts at: 4811 4811 Size: 6007 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6007 BstXI CCAn_nnnn'nTGG Cuts at: 2623 2656 3092 2623 Size: 33 436 5538 Fragments arranged by size: 5538 436 33 BstYI r'GATC_y Cuts at: 1972 2019 2034 2884 3337 3704 4089 4106 5558 Size: 47 15 850 453 367 385 17 1452 Cuts at: 5558 5644 5656 6002 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 850 453 385 367 346 86 47 17 15 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5455 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2389 3130 487 Size: 683 56 1163 741 3364 Fragments arranged by size: 3364 1163 741 683 56 BtsI GCAGTG_nn' Cuts at: 3523 4317 4337 3523 Size: 794 20 5193 Fragments arranged by size: 5193 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6007 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5972 DraI TTT'AAA Cuts at: 3311 3311 Size: 6007 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5014 809 Size: 507 3698 1802 Fragments arranged by size: 3698 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2493 3216 3300 Size: 54 1425 50 275 18 663 723 84 Cuts at: 3300 3454 4343 5972 8 Size: 154 889 1629 43 Fragments arranged by size: 1629 1425 889 723 663 275 154 84 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3454 1537 Size: 275 1642 4090 Fragments arranged by size: 4090 1642 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2081 2099 3069 3240 3937 1353 Size: 728 18 970 171 697 3423 Fragments arranged by size: 3423 970 728 697 171 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4594 4968 5114 387 Size: 1064 138 3005 374 146 1280 Fragments arranged by size: 3005 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2149 2149 Size: 6007 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2080 2448 2521 2600 2678 2696 2705 Size: 393 448 368 73 79 78 18 9 Cuts at: 2705 2743 3050 3153 3271 4070 4667 5454 5722 Size: 38 307 103 118 799 597 787 268 Cuts at: 5722 1239 Size: 1524 Fragments arranged by size: 1524 799 787 597 448 393 368 307 268 118 103 79 78 73 38 18 9 EcoO109I rG'GnC_Cy Cuts at: 2140 2386 2140 Size: 246 5761 EcoRI G'AATT_C Cuts at: 3316 3694 3316 Size: 378 5629 FspI TGC'GCA Cuts at: 2552 4513 2552 Size: 1961 4046 FspAI rTGC'GCAy Cuts at: 2552 2552 Size: 6007 HaeII r_GCGC'y Cuts at: 1678 3214 5154 1678 Size: 1536 1940 2531 Fragments arranged by size: 2531 1940 1536 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2774 2806 3046 3078 4648 Size: 32 135 32 1202 32 240 32 1570 Cuts at: 4648 4680 4722 4754 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1570 1202 240 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3037 3351 3597 669 Size: 988 281 1099 314 246 3079 Fragments arranged by size: 3079 1099 988 314 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6007 HpaI GTT'AAC Cuts at: 3597 3597 Size: 6007 KpnI G_GTAC'C Cuts at: 3332 3332 Size: 6007 MfeI C'AATT_G Cuts at: 3606 3606 Size: 6007 MluI A'CGCG_T Cuts at: 5883 5883 Size: 6007 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5121 5305 1305 Size: 3816 184 2007 Fragments arranged by size: 3816 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2495 3218 3302 10 Size: 54 1425 343 663 723 84 2715 Fragments arranged by size: 2715 1425 723 663 343 84 54 MslI CAynn'nnrTG Cuts at: 518 1166 2621 2732 3965 4324 4483 518 Size: 648 1455 111 1233 359 159 2042 Fragments arranged by size: 2042 1455 1233 648 359 159 111 MspA1I CmG'CkG Cuts at: 1606 2830 3423 4100 5248 5493 1606 Size: 1224 593 677 1148 245 2120 Fragments arranged by size: 2120 1224 1148 677 593 245 NaeI GCC'GGC Cuts at: 3277 3277 Size: 6007 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5455 NdeI CA'TA_TG Cuts at: 387 387 Size: 6007 NgoMIV G'CCGG_C Cuts at: 3275 3275 Size: 6007 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6007 NotI GC'GGCC_GC Cuts at: 3454 3454 Size: 6007 NspI r_CATG'y Cuts at: 1132 1229 3365 4910 1132 Size: 97 2136 1545 2229 Fragments arranged by size: 2229 2136 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6007 PciI A'CATG_T Cuts at: 4906 4906 Size: 6007 PflMI CCAn_nnn'nTGG Cuts at: 1108 2950 3096 1108 Size: 1842 146 4019 Fragments arranged by size: 4019 1842 146 PfoI T'CCnGG_A Cuts at: 2151 2655 2151 Size: 504 5503 PmeI GTTT'AAAC Cuts at: 3311 3311 Size: 6007 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4076 4108 5589 5621 4076 Size: 32 1481 32 4462 Fragments arranged by size: 4462 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2386 2386 Size: 6007 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6007 PsiI TTA'TAA Cuts at: 3577 3577 Size: 6007 PstI C_TGCA'G Cuts at: 830 3359 830 Size: 2529 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6007 PvuII CAG'CTG Cuts at: 2830 2830 Size: 6007 SacI G_AGCT'C Cuts at: 2151 2151 Size: 6007 SalI G'TCGA_C Cuts at: 1936 3349 1936 Size: 1413 4594 SanDI GG'GwC_CC Cuts at: 2386 2386 Size: 6007 SbfI CC_TGCA'GG Cuts at: 3359 3359 Size: 6007 ScaI AGT'ACT Cuts at: 4255 5967 4255 Size: 1712 4295 SexAI A'CCwGG_T Cuts at: 3153 3153 Size: 6007 SfcI C'TryA_G Cuts at: 826 1045 3355 4490 5171 5362 826 Size: 219 2310 1135 681 191 1471 Fragments arranged by size: 2310 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6007 SmaI CCC'GGG Cuts at: 1872 3334 1872 Size: 1462 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2015 4111 5012 5274 820 Size: 197 926 72 2096 901 262 1553 Fragments arranged by size: 2096 1553 926 901 262 197 72 SnaBI TAC'GTA Cuts at: 493 3798 493 Size: 3305 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6007 SphI G_CATG'C Cuts at: 1132 3365 1132 Size: 2233 3774 SspI AAT'ATT Cuts at: 5 52 3931 5 Size: 47 3879 2081 Fragments arranged by size: 3879 2081 47 StuI AGG'CCT Cuts at: 1517 2673 1517 Size: 1156 4851 StyI C'CwwG_G Cuts at: 513 1065 2991 3297 3303 3446 513 Size: 552 1926 306 6 143 3074 Fragments arranged by size: 3074 1926 552 306 143 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3071 4034 4051 4204 4389 4834 325 Size: 1255 1491 963 17 153 185 445 1498 Fragments arranged by size: 1498 1491 1255 963 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2841 3222 4253 Size: 274 80 33 51 1443 864 381 1031 Cuts at: 4253 5965 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1031 864 381 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5967 5999 5967 Size: 32 5975 Tth111I GACn'n_nGTC Cuts at: 1316 3058 1316 Size: 1742 4265 XbaI T'CTAG_A Cuts at: 3343 3343 Size: 6007 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6007 XmaI C'CCGG_G Cuts at: 1870 3332 1870 Size: 1462 4545 XmnI GAAnn'nnTTC Cuts at: 4136 4136 Size: 6007 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3815 276 Size: 53 83 186 936 2281 2468 Fragments arranged by size: 2468 2281 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI XcmI