(Circular) (Six-base) MAPSORT of: pFN21AB5348.seq Check: 6265 from: 1 to: 5875 pFN21AB5348 5875 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3685 278 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2281 3218 1937 Size: 344 937 4594 Fragments arranged by size: 4594 937 344 Acc65I G'GTAC_C Cuts at: 3069 3196 3069 Size: 127 5748 AclI AA'CG_TT Cuts at: 1563 4002 4375 1563 Size: 2439 373 3063 Fragments arranged by size: 3063 2439 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2490 3938 5322 5590 1632 Size: 858 1448 1384 268 1917 Fragments arranged by size: 1917 1448 1384 858 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5678 AflIII A'CryG_T Cuts at: 1203 4774 5751 1203 Size: 3571 977 1327 Fragments arranged by size: 3571 1327 977 AgeI A'CCGG_T Cuts at: 4706 4706 Size: 5875 AhdI GACnn_n'nnGTC Cuts at: 4604 4604 Size: 5875 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5875 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5875 AlwNI CAG_nnn'CTG Cuts at: 3618 5190 3618 Size: 1572 4303 ApaI G_GGCC'C Cuts at: 2904 2904 Size: 5875 ApaLI G'TGCA_C Cuts at: 3932 5088 3932 Size: 1156 4719 ApoI r'AATT_y Cuts at: 1493 2413 3184 3410 3562 5671 1493 Size: 920 771 226 152 2109 1697 Fragments arranged by size: 2109 1697 920 771 226 152 AseI AT'TA_AT Cuts at: 160 1032 4429 160 Size: 872 3397 1606 Fragments arranged by size: 3397 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 AvaI C'yCGr_G Cuts at: 1870 1943 3200 1870 Size: 73 1257 4545 Fragments arranged by size: 4545 1257 73 BamHI G'GATC_C Cuts at: 3205 3572 3205 Size: 367 5508 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3069 3196 4651 618 Size: 325 217 627 1282 127 1455 1842 Fragments arranged by size: 1842 1455 1282 627 325 217 127 BanII G_rGCy'C Cuts at: 1391 2548 2904 3194 1391 Size: 1157 356 290 4072 Fragments arranged by size: 4072 1157 356 290 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4904 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4066 4100 1903 Size: 34 2129 34 3678 Fragments arranged by size: 3678 2129 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3767 4977 3767 Size: 1210 4665 BclI T'GATC_A Cuts at: 1548 1548 Size: 5875 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4486 136 Size: 107 122 71 631 605 2814 1525 Fragments arranged by size: 2814 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2495 5870 2495 Size: 3375 2500 BlpI GC'TnA_GC Cuts at: 3292 3292 Size: 5875 Bme1580I G_kGCm'C Cuts at: 1790 2904 3936 5092 1790 Size: 1114 1032 1156 2573 Fragments arranged by size: 2573 1156 1114 1032 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5875 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2441 3606 4564 Size: 416 53 83 399 24 1024 1165 958 Cuts at: 4564 442 Size: 1753 Fragments arranged by size: 1753 1165 1024 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5875 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3153 4535 1239 Size: 1914 1382 2579 Fragments arranged by size: 2579 1914 1382 Bpu10I CC'TnA_GC Cuts at: 2190 2190 Size: 5875 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2971 2995 4000 4865 5163 2971 Size: 24 1005 865 298 3683 Fragments arranged by size: 3683 1005 865 298 24 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4538 882 Size: 617 3039 2219 Fragments arranged by size: 3039 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2558 2759 2879 2954 3083 3666 493 Size: 711 1354 201 120 75 129 583 2702 Fragments arranged by size: 2702 1354 711 583 201 129 120 75 BsaBI GATnn'nnATC Cuts at: 2766 5869 2766 Size: 3103 2772 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3682 4064 4699 275 Size: 53 83 186 936 2149 382 635 1451 Fragments arranged by size: 2149 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4308 4706 4980 5127 5538 4308 Size: 398 274 147 411 4645 Fragments arranged by size: 4645 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5650 Fragments arranged by size: 5650 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2358 2394 2538 1186 Size: 1172 36 144 4523 Fragments arranged by size: 4523 1172 144 36 BseYI C'CCAG_C Cuts at: 2179 5078 5574 2179 Size: 2899 496 2480 Fragments arranged by size: 2899 2480 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2484 1163 Size: 573 748 4554 Fragments arranged by size: 4554 748 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3325 4086 5114 1540 Size: 275 186 1324 761 1028 2301 Fragments arranged by size: 2301 1324 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2548 2887 3115 3936 4021 5092 2548 Size: 339 228 821 85 1071 3331 Fragments arranged by size: 3331 1071 821 339 228 85 BsmI GAATG_Cn' Cuts at: 3386 3479 3386 Size: 93 5782 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2548 2887 2904 3115 3194 3936 4021 Size: 399 758 339 17 211 79 742 85 Cuts at: 4021 5092 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 758 742 399 339 211 85 79 17 BspEI T'CCGG_A Cuts at: 5538 5538 Size: 5875 BspHI T'CATG_A Cuts at: 3764 5494 3764 Size: 1730 4145 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3230 844 Size: 2386 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2599 3762 1394 Size: 429 776 1163 3507 Fragments arranged by size: 3507 1163 776 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4370 4544 65 Size: 1183 3122 174 1396 Fragments arranged by size: 3122 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4519 4706 1700 Size: 110 2709 187 2869 Fragments arranged by size: 2869 2709 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5875 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5875 BssSI C'ACGA_G Cuts at: 2376 2688 2718 2895 3935 4947 2376 Size: 312 30 177 1040 1012 3304 Fragments arranged by size: 3304 1040 1012 312 177 30 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5875 BstBI TT'CG_AA Cuts at: 4679 4679 Size: 5875 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5875 BstXI CCAn_nnnn'nTGG Cuts at: 2245 2245 Size: 5875 BstYI r'GATC_y Cuts at: 1972 2495 3205 3572 3957 3974 5426 5512 5524 Size: 523 710 367 385 17 1452 86 12 Cuts at: 5524 5870 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 710 523 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3106 513 Size: 552 939 1102 3282 Fragments arranged by size: 3282 1102 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5136 Fragments arranged by size: 5136 683 56 BtsI GCAGTG_nn' Cuts at: 2533 3391 4185 4205 2533 Size: 858 794 20 4203 Fragments arranged by size: 4203 858 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5875 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5840 DraI TTT'AAA Cuts at: 3179 3179 Size: 5875 DraIII CAC_nnn'GTG Cuts at: 2882 2882 Size: 5875 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4882 809 Size: 507 3566 1802 Fragments arranged by size: 3566 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3322 4211 5840 Size: 54 1425 50 275 18 1492 889 1629 Cuts at: 5840 8 Size: 43 Fragments arranged by size: 1629 1492 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3322 1537 Size: 275 1510 4090 Fragments arranged by size: 4090 1510 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2039 2335 2536 2557 3805 1353 Size: 686 296 201 21 1248 3423 Fragments arranged by size: 3423 1248 686 296 201 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4462 4836 4982 387 Size: 1064 138 2873 374 146 1280 Fragments arranged by size: 2873 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2546 2546 Size: 5875 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2490 3153 3938 4535 5322 5590 1239 Size: 393 858 663 785 597 787 268 1524 Fragments arranged by size: 1524 858 787 785 663 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2322 2376 2322 Size: 54 5821 EcoO109I rG'GnC_Cy Cuts at: 2315 2366 2480 2900 2901 2315 Size: 51 114 420 1 5289 Fragments arranged by size: 5289 420 114 51 1 EcoRI G'AATT_C Cuts at: 3184 3562 3184 Size: 378 5497 FspI TGC'GCA Cuts at: 4381 4381 Size: 5875 HaeII r_GCGC'y Cuts at: 1678 5022 1678 Size: 3344 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2756 2788 2969 2993 3001 Size: 32 135 32 1184 32 181 24 8 Cuts at: 3001 3025 4516 4548 4590 4622 1373 Size: 24 1491 32 42 32 2626 Fragments arranged by size: 2626 1491 1184 181 135 42 32 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 3219 3465 669 Size: 988 281 1281 246 3079 Fragments arranged by size: 3079 1281 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5875 HpaI GTT'AAC Cuts at: 3465 3465 Size: 5875 KpnI G_GTAC'C Cuts at: 3073 3200 3073 Size: 127 5748 MfeI C'AATT_G Cuts at: 3474 3474 Size: 5875 MluI A'CGCG_T Cuts at: 5751 5751 Size: 5875 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4989 5173 1305 Size: 3684 184 2007 Fragments arranged by size: 3684 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4053 Fragments arranged by size: 4053 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2243 2391 2832 3833 4192 4351 518 Size: 648 1077 148 441 1001 359 159 2042 Fragments arranged by size: 2042 1077 1001 648 441 359 159 148 MspA1I CmG'CkG Cuts at: 1606 3108 3291 3968 5116 5361 1606 Size: 1502 183 677 1148 245 2120 Fragments arranged by size: 2120 1502 1148 677 245 183 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4384 Fragments arranged by size: 4384 939 552 NdeI CA'TA_TG Cuts at: 387 2857 387 Size: 2470 3405 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5875 NotI GC'GGCC_GC Cuts at: 3322 3322 Size: 5875 NspI r_CATG'y Cuts at: 1132 1229 3233 4778 1132 Size: 97 2004 1545 2229 Fragments arranged by size: 2229 2004 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5875 PciI A'CATG_T Cuts at: 4774 4774 Size: 5875 PflMI CCAn_nnn'nTGG Cuts at: 1108 2474 2915 1108 Size: 1366 441 4068 Fragments arranged by size: 4068 1366 441 PmeI GTTT'AAAC Cuts at: 3179 3179 Size: 5875 PmlI CAC'GTG Cuts at: 2558 2759 2954 3083 2558 Size: 201 195 129 5350 Fragments arranged by size: 5350 201 195 129 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3944 3976 5457 5489 3944 Size: 32 1481 32 4330 Fragments arranged by size: 4330 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2315 2366 2480 2315 Size: 51 114 5710 Fragments arranged by size: 5710 114 51 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5875 PsiI TTA'TAA Cuts at: 3445 3445 Size: 5875 PspOMI G'GGCC_C Cuts at: 2900 2900 Size: 5875 PstI C_TGCA'G Cuts at: 830 3227 830 Size: 2397 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5875 SacI G_AGCT'C Cuts at: 2548 2548 Size: 5875 SacII CC_GC'GG Cuts at: 3109 3109 Size: 5875 SalI G'TCGA_C Cuts at: 1936 3217 1936 Size: 1281 4594 SapI GCTCTTCn'nnn_ Cuts at: 2039 2536 2039 Size: 497 5378 SbfI CC_TGCA'GG Cuts at: 3227 3227 Size: 5875 ScaI AGT'ACT Cuts at: 2115 4123 5835 2115 Size: 2008 1712 2155 Fragments arranged by size: 2155 2008 1712 SfcI C'TryA_G Cuts at: 826 1045 2845 3057 3091 3223 4358 5039 5230 Size: 219 1800 212 34 132 1135 681 191 Cuts at: 5230 826 Size: 1471 Fragments arranged by size: 1800 1471 1135 681 219 212 191 132 34 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5875 SmaI CCC'GGG Cuts at: 1872 3202 1872 Size: 1330 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2986 3010 3979 4880 5142 820 Size: 197 926 1043 24 969 901 262 1553 Fragments arranged by size: 1553 1043 969 926 901 262 197 24 SnaBI TAC'GTA Cuts at: 493 3666 493 Size: 3173 2702 SpeI A'CTAG_T Cuts at: 152 2512 152 Size: 2360 3515 SphI G_CATG'C Cuts at: 1132 3233 1132 Size: 2101 3774 SspI AAT'ATT Cuts at: 5 52 3799 5 Size: 47 3747 2081 Fragments arranged by size: 3747 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5875 StyI C'CwwG_G Cuts at: 513 1065 2004 3314 513 Size: 552 939 1310 3074 Fragments arranged by size: 3074 1310 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3902 3919 4072 4257 4702 325 Size: 1255 2322 17 153 185 445 1498 Fragments arranged by size: 2322 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2113 4121 5833 Size: 274 80 33 51 1443 136 2008 1712 Cuts at: 5833 96 Size: 138 Fragments arranged by size: 2008 1712 1443 274 138 136 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2091 2123 5835 5867 2091 Size: 32 3712 32 2099 Fragments arranged by size: 3712 2099 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5875 XbaI T'CTAG_A Cuts at: 3211 3211 Size: 5875 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5875 XmaI C'CCGG_G Cuts at: 1870 3200 1870 Size: 1330 4545 XmnI GAAnn'nnTTC Cuts at: 4004 4004 Size: 5875 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3683 276 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI PvuII RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI