(Circular) (Six-base) MAPSORT of: pFN21AB5318.seq Check: 9961 from: 1 to: 5980 pFN21AB5318 5980 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2718 2718 Size: 5980 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3790 278 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3323 1937 Size: 1386 4594 Acc65I G'GTAC_C Cuts at: 3301 3301 Size: 5980 AclI AA'CG_TT Cuts at: 1563 4107 4480 1563 Size: 2544 373 3063 Fragments arranged by size: 3063 2544 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2105 2175 2190 4043 5427 5695 1632 Size: 473 70 15 1853 1384 268 1917 Fragments arranged by size: 1917 1853 1384 473 268 70 15 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5783 AflIII A'CryG_T Cuts at: 1203 4879 5856 1203 Size: 3676 977 1327 Fragments arranged by size: 3676 1327 977 AgeI A'CCGG_T Cuts at: 4811 4811 Size: 5980 AhdI GACnn_n'nnGTC Cuts at: 2773 4709 2773 Size: 1936 4044 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5980 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5980 AlwNI CAG_nnn'CTG Cuts at: 3182 3723 5295 3182 Size: 541 1572 3867 Fragments arranged by size: 3867 1572 541 ApaLI G'TGCA_C Cuts at: 4037 5193 4037 Size: 1156 4824 ApoI r'AATT_y Cuts at: 1493 2312 2557 2563 2858 2958 3289 3515 3667 Size: 819 245 6 295 100 331 226 152 Cuts at: 3667 5776 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 819 331 295 245 226 152 100 6 AseI AT'TA_AT Cuts at: 160 1032 4534 160 Size: 872 3502 1606 Fragments arranged by size: 3502 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 AvaI C'yCGr_G Cuts at: 1870 1943 2247 3305 1870 Size: 73 304 1058 4545 Fragments arranged by size: 4545 1058 304 73 BamHI G'GATC_C Cuts at: 3310 3677 3310 Size: 367 5613 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2706 3301 4756 618 Size: 325 217 627 919 595 1455 1842 Fragments arranged by size: 1842 1455 919 627 595 325 217 BanII G_rGCy'C Cuts at: 1391 3299 1391 Size: 1908 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5009 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4171 4205 1903 Size: 34 2234 34 3678 Fragments arranged by size: 3678 2234 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3872 5082 3872 Size: 1210 4770 BclI T'GATC_A Cuts at: 1548 2746 1548 Size: 1198 4782 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4591 136 Size: 107 122 71 631 605 2919 1525 Fragments arranged by size: 2919 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5975 5975 Size: 5980 BlpI GC'TnA_GC Cuts at: 3397 3397 Size: 5980 Bme1580I G_kGCm'C Cuts at: 1790 4041 5197 1790 Size: 2251 1156 2573 Fragments arranged by size: 2573 2251 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5980 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2124 3711 4669 Size: 416 53 83 399 24 707 1587 958 Cuts at: 4669 442 Size: 1753 Fragments arranged by size: 1753 1587 958 707 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5980 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3075 3284 4640 1239 Size: 1836 209 1356 2579 Fragments arranged by size: 2579 1836 1356 209 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4105 4970 5268 4105 Size: 865 298 4817 Fragments arranged by size: 4817 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2552 2982 4643 882 Size: 617 1053 430 1661 2219 Fragments arranged by size: 2219 1661 1053 617 430 BsaAI yAC'GTr Cuts at: 493 1204 3771 493 Size: 711 2567 2702 Fragments arranged by size: 2702 2567 711 BsaBI GATnn'nnATC Cuts at: 5974 5974 Size: 5980 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3787 4169 4804 275 Size: 53 83 186 936 2254 382 635 1451 Fragments arranged by size: 2254 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4413 4811 5085 5232 5643 4413 Size: 398 274 147 411 4750 Fragments arranged by size: 4750 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5755 Fragments arranged by size: 5755 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 1186 Size: 868 5112 BseYI C'CCAG_C Cuts at: 2203 5183 5679 2203 Size: 2980 496 2504 Fragments arranged by size: 2980 2504 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2699 1163 Size: 573 963 4444 Fragments arranged by size: 4444 963 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3430 4191 5219 1540 Size: 275 186 1429 761 1028 2301 Fragments arranged by size: 2301 1429 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2392 4041 4126 5197 2392 Size: 1649 85 1071 3175 Fragments arranged by size: 3175 1649 1071 85 BsmI GAATG_Cn' Cuts at: 3491 3584 3491 Size: 93 5887 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2392 3299 4041 4126 5197 1391 Size: 399 602 907 742 85 1071 2174 Fragments arranged by size: 2174 1071 907 742 602 399 85 BspEI T'CCGG_A Cuts at: 5643 5643 Size: 5980 BspHI T'CATG_A Cuts at: 3869 5599 3869 Size: 1730 4250 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2718 3335 844 Size: 1874 617 3489 Fragments arranged by size: 3489 1874 617 BsrBI CCG'CTC Cuts at: 1394 1823 3867 1394 Size: 429 2044 3507 Fragments arranged by size: 3507 2044 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2418 4475 4649 65 Size: 1183 1170 2057 174 1396 Fragments arranged by size: 2057 1396 1183 1170 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2873 4624 4811 1700 Size: 110 1063 1751 187 2869 Fragments arranged by size: 2869 1751 1063 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5980 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5980 BssSI C'ACGA_G Cuts at: 2717 4040 5052 2717 Size: 1323 1012 3645 Fragments arranged by size: 3645 1323 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2713 3074 794 Size: 1919 361 3700 Fragments arranged by size: 3700 1919 361 BstBI TT'CG_AA Cuts at: 2940 4784 2940 Size: 1844 4136 BstEII G'GTnAC_C Cuts at: 1184 2525 1184 Size: 1341 4639 BstYI r'GATC_y Cuts at: 1972 2732 2905 3275 3310 3677 4062 4079 5531 Size: 760 173 370 35 367 385 17 1452 Cuts at: 5531 5617 5629 5975 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 760 385 370 367 346 173 86 35 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4489 Fragments arranged by size: 4489 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5241 Fragments arranged by size: 5241 683 56 BtsI GCAGTG_nn' Cuts at: 2817 3120 3496 4290 4310 2817 Size: 303 376 794 20 4487 Fragments arranged by size: 4487 794 376 303 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5980 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5945 DraI TTT'AAA Cuts at: 3284 3284 Size: 5980 DraIII CAC_nnn'GTG Cuts at: 2806 2806 Size: 5980 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2215 4987 809 Size: 507 899 2772 1802 Fragments arranged by size: 2772 1802 899 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2674 3427 4316 Size: 54 1425 50 275 18 844 753 889 Cuts at: 4316 5945 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 844 753 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3427 1537 Size: 275 1615 4090 Fragments arranged by size: 4090 1615 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2124 3231 3910 1353 Size: 771 1107 679 3423 Fragments arranged by size: 3423 1107 771 679 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4567 4941 5087 387 Size: 1064 138 2978 374 146 1280 Fragments arranged by size: 2978 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2175 2190 3075 3284 4043 4640 Size: 393 473 70 15 885 209 759 597 Cuts at: 4640 5427 5695 1239 Size: 787 268 1524 Fragments arranged by size: 1524 885 787 759 597 473 393 268 209 70 15 EcoRI G'AATT_C Cuts at: 2557 3289 3667 2557 Size: 732 378 4870 Fragments arranged by size: 4870 732 378 FspI TGC'GCA Cuts at: 2814 4486 2814 Size: 1672 4308 FspAI rTGC'GCAy Cuts at: 2814 2814 Size: 5980 HaeII r_GCGC'y Cuts at: 1678 5127 1678 Size: 3449 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2101 2133 2560 2592 2759 Size: 32 135 32 529 32 427 32 167 Cuts at: 2759 2791 3224 3256 4621 4653 4695 4727 1373 Size: 32 433 32 1365 32 42 32 2626 Fragments arranged by size: 2626 1365 529 433 427 167 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2830 3324 3570 669 Size: 988 281 892 494 246 3079 Fragments arranged by size: 3079 988 892 494 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5980 HpaI GTT'AAC Cuts at: 3570 3570 Size: 5980 KpnI G_GTAC'C Cuts at: 3305 3305 Size: 5980 MfeI C'AATT_G Cuts at: 3579 3579 Size: 5980 MluI A'CGCG_T Cuts at: 5856 5856 Size: 5980 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5094 5278 1305 Size: 3789 184 2007 Fragments arranged by size: 3789 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2676 10 Size: 54 1425 343 844 3314 Fragments arranged by size: 3314 1425 844 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2952 3938 4297 4456 518 Size: 648 1786 986 359 159 2042 Fragments arranged by size: 2042 1786 986 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2684 3396 4073 5221 5466 1606 Size: 1078 712 677 1148 245 2120 Fragments arranged by size: 2120 1148 1078 712 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4489 Fragments arranged by size: 4489 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5980 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5980 NotI GC'GGCC_GC Cuts at: 3427 3427 Size: 5980 NspI r_CATG'y Cuts at: 1132 1229 2401 3338 4883 1132 Size: 97 1172 937 1545 2229 Fragments arranged by size: 2229 1545 1172 937 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5980 PciI A'CATG_T Cuts at: 4879 4879 Size: 5980 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5980 PfoI T'CCnGG_A Cuts at: 2552 2552 Size: 5980 PmeI GTTT'AAAC Cuts at: 3284 3284 Size: 5980 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4049 4081 5562 5594 4049 Size: 32 1481 32 4435 Fragments arranged by size: 4435 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5980 PsiI TTA'TAA Cuts at: 3550 3550 Size: 5980 PspXI vC'TCGA_Gb Cuts at: 2247 2247 Size: 5980 PstI C_TGCA'G Cuts at: 830 3332 830 Size: 2502 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5980 PvuII CAG'CTG Cuts at: 2684 2684 Size: 5980 SalI G'TCGA_C Cuts at: 1936 3322 1936 Size: 1386 4594 SapI GCTCTTCn'nnn_ Cuts at: 2124 2124 Size: 5980 SbfI CC_TGCA'GG Cuts at: 3332 3332 Size: 5980 ScaI AGT'ACT Cuts at: 4228 5940 4228 Size: 1712 4268 SexAI A'CCwGG_T Cuts at: 2100 2771 2100 Size: 671 5309 SfcI C'TryA_G Cuts at: 826 1045 2851 3328 4463 5144 5335 826 Size: 219 1806 477 1135 681 191 1471 Fragments arranged by size: 1806 1471 1135 681 477 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5980 SmaI CCC'GGG Cuts at: 1872 3307 1872 Size: 1435 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2247 4084 4985 5247 820 Size: 197 926 304 1837 901 262 1553 Fragments arranged by size: 1837 1553 926 901 304 262 197 SnaBI TAC'GTA Cuts at: 493 3771 493 Size: 3278 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5980 SphI G_CATG'C Cuts at: 1132 3338 1132 Size: 2206 3774 SspI AAT'ATT Cuts at: 5 52 3223 3904 5 Size: 47 3171 681 2081 Fragments arranged by size: 3171 2081 681 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5980 StyI C'CwwG_G Cuts at: 513 1065 2004 3161 3419 513 Size: 552 939 1157 258 3074 Fragments arranged by size: 3074 1157 939 552 258 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4007 4024 4177 4362 4807 325 Size: 1255 2427 17 153 185 445 1498 Fragments arranged by size: 2427 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2251 4226 5938 Size: 274 80 33 51 1443 274 1975 1712 Cuts at: 5938 96 Size: 138 Fragments arranged by size: 1975 1712 1443 274 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5940 5972 5940 Size: 32 5948 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5980 XbaI T'CTAG_A Cuts at: 3316 3316 Size: 5980 XhoI C'TCGA_G Cuts at: 1943 2247 1943 Size: 304 5676 XmaI C'CCGG_G Cuts at: 1870 3305 1870 Size: 1435 4545 XmnI GAAnn'nnTTC Cuts at: 2862 2994 4109 2862 Size: 132 1115 4733 Fragments arranged by size: 4733 1115 132 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3788 276 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI