(Circular) (Six-base) MAPSORT of: pFN21AB5311.seq Check: 5973 from: 1 to: 6103 pFN21AB5311 6103 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3913 278 Size: 53 83 186 936 2377 2468 Fragments arranged by size: 2468 2377 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3446 1937 Size: 1509 4594 Acc65I G'GTAC_C Cuts at: 3424 3424 Size: 6103 AclI AA'CG_TT Cuts at: 1563 4230 4603 1563 Size: 2667 373 3063 Fragments arranged by size: 3063 2667 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2282 2576 4166 5550 5818 1632 Size: 650 294 1590 1384 268 1917 Fragments arranged by size: 1917 1590 1384 650 294 268 AflII C'TTAA_G Cuts at: 820 1017 3332 820 Size: 197 2315 3591 Fragments arranged by size: 3591 2315 197 AflIII A'CryG_T Cuts at: 1203 2142 3075 5002 5979 1203 Size: 939 933 1927 977 1327 Fragments arranged by size: 1927 1327 977 939 933 AgeI A'CCGG_T Cuts at: 4934 4934 Size: 6103 AhdI GACnn_n'nnGTC Cuts at: 4832 4832 Size: 6103 AleI CACnn'nnGTG Cuts at: 1166 2375 1166 Size: 1209 4894 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6103 AlwNI CAG_nnn'CTG Cuts at: 2108 3157 3247 3846 5418 2108 Size: 1049 90 599 1572 2793 Fragments arranged by size: 2793 1572 1049 599 90 ApaLI G'TGCA_C Cuts at: 3101 4160 5316 3101 Size: 1059 1156 3888 Fragments arranged by size: 3888 1156 1059 ApoI r'AATT_y Cuts at: 1493 2298 3089 3412 3638 3790 5899 1493 Size: 805 791 323 226 152 2109 1697 Fragments arranged by size: 2109 1697 805 791 323 226 152 AseI AT'TA_AT Cuts at: 160 1032 4657 160 Size: 872 3625 1606 Fragments arranged by size: 3625 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6103 AvaI C'yCGr_G Cuts at: 1870 1943 2722 3428 1870 Size: 73 779 706 4545 Fragments arranged by size: 4545 779 706 73 BamHI G'GATC_C Cuts at: 3433 3800 3433 Size: 367 5736 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3083 3382 3424 4879 618 Size: 325 217 627 1296 299 42 1455 1842 Fragments arranged by size: 1842 1455 1296 627 325 299 217 42 BanII G_rGCy'C Cuts at: 1391 2247 3150 3422 1391 Size: 856 903 272 4072 Fragments arranged by size: 4072 903 856 272 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2462 2858 928 Size: 971 563 396 4173 Fragments arranged by size: 4173 971 563 396 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3165 3199 4294 4328 1903 Size: 34 1228 34 1095 34 3678 Fragments arranged by size: 3678 1228 1095 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3995 5205 3995 Size: 1210 4893 BclI T'GATC_A Cuts at: 1548 2794 1548 Size: 1246 4857 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4714 136 Size: 107 122 71 631 605 3042 1525 Fragments arranged by size: 3042 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6098 6098 Size: 6103 BlpI GC'TnA_GC Cuts at: 2912 3520 2912 Size: 608 5495 Bme1580I G_kGCm'C Cuts at: 1790 3105 3224 4164 5320 1790 Size: 1315 119 940 1156 2573 Fragments arranged by size: 2573 1315 1156 940 119 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6103 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2577 3834 4792 Size: 416 53 83 399 24 1160 1257 958 Cuts at: 4792 442 Size: 1753 Fragments arranged by size: 1753 1257 1160 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6103 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 4763 1239 Size: 854 2670 2579 Fragments arranged by size: 2670 2579 854 Bpu10I CC'TnA_GC Cuts at: 2667 2667 Size: 6103 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1997 4228 5093 5391 1997 Size: 2231 865 298 2709 Fragments arranged by size: 2709 2231 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2163 4766 882 Size: 617 664 2603 2219 Fragments arranged by size: 2603 2219 664 617 BsaAI yAC'GTr Cuts at: 493 1204 2341 3894 493 Size: 711 1137 1553 2702 Fragments arranged by size: 2702 1553 1137 711 BsaBI GATnn'nnATC Cuts at: 3131 6097 3131 Size: 2966 3137 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3910 4292 4927 275 Size: 53 83 186 936 2377 382 635 1451 Fragments arranged by size: 2377 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2923 4536 4934 5208 5355 5766 2923 Size: 1613 398 274 147 411 3260 Fragments arranged by size: 3260 1613 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5878 Fragments arranged by size: 5878 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2623 3029 1186 Size: 1437 406 4260 Fragments arranged by size: 4260 1437 406 BseYI C'CCAG_C Cuts at: 5306 5802 5306 Size: 496 5607 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2460 3385 1163 Size: 573 724 925 3881 Fragments arranged by size: 3881 925 724 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2963 3553 4314 5342 1540 Size: 275 186 962 590 761 1028 2301 Fragments arranged by size: 2301 1028 962 761 590 275 186 BsiHKAI G_wGCw'C Cuts at: 3105 4164 4249 5320 3105 Size: 1059 85 1071 3888 Fragments arranged by size: 3888 1071 1059 85 BsmI GAATG_Cn' Cuts at: 2840 2908 3614 3707 2840 Size: 68 706 93 5236 Fragments arranged by size: 5236 706 93 68 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2247 3105 3150 3224 3422 4164 4249 Size: 399 457 858 45 74 198 742 85 Cuts at: 4249 5320 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 858 742 457 399 198 85 74 45 BspEI T'CCGG_A Cuts at: 2923 5766 2923 Size: 2843 3260 BspHI T'CATG_A Cuts at: 2490 3992 5722 2490 Size: 1502 1730 2871 Fragments arranged by size: 2871 1730 1502 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3458 844 Size: 2614 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3990 1394 Size: 429 2167 3507 Fragments arranged by size: 3507 2167 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3201 4598 4772 65 Size: 1183 1953 1397 174 1396 Fragments arranged by size: 1953 1397 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4747 4934 1700 Size: 110 2937 187 2869 Fragments arranged by size: 2937 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2145 96 Size: 2049 4054 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6103 BssSI C'ACGA_G Cuts at: 4163 5175 4163 Size: 1012 5091 BstAPI GCAn_nnn'nTGC Cuts at: 794 2083 3247 794 Size: 1289 1164 3650 Fragments arranged by size: 3650 1289 1164 BstBI TT'CG_AA Cuts at: 4907 4907 Size: 6103 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6103 BstXI CCAn_nnnn'nTGG Cuts at: 2881 2881 Size: 6103 BstYI r'GATC_y Cuts at: 1972 2352 3108 3433 3800 4185 4202 5654 5740 Size: 380 756 325 367 385 17 1452 86 Cuts at: 5740 5752 6098 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 756 385 380 367 346 325 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2247 513 Size: 552 1182 4369 Fragments arranged by size: 4369 1182 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2426 487 Size: 683 56 1200 4164 Fragments arranged by size: 4164 1200 683 56 BtsI GCAGTG_nn' Cuts at: 3619 4413 4433 3619 Size: 794 20 5289 Fragments arranged by size: 5289 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6103 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6068 DraI TTT'AAA Cuts at: 3407 3407 Size: 6103 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5110 809 Size: 507 3794 1802 Fragments arranged by size: 3794 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2099 2292 2772 Size: 54 1425 50 275 18 269 193 480 Cuts at: 2772 2960 3550 4439 6068 8 Size: 188 590 889 1629 43 Fragments arranged by size: 1629 1425 889 590 480 275 269 193 188 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2960 3550 1537 Size: 275 1148 590 4090 Fragments arranged by size: 4090 1148 590 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2235 2966 4033 1353 Size: 882 731 1067 3423 Fragments arranged by size: 3423 1067 882 731 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4690 5064 5210 387 Size: 1064 138 3101 374 146 1280 Fragments arranged by size: 3101 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2282 2576 4166 4763 5550 5818 Size: 393 461 189 294 1590 597 787 268 Cuts at: 5818 1239 Size: 1524 Fragments arranged by size: 1590 1524 787 597 461 393 294 268 189 EcoNI CCTnn'n_nnAGG Cuts at: 2166 2166 Size: 6103 EcoRI G'AATT_C Cuts at: 2298 3412 3790 2298 Size: 1114 378 4611 Fragments arranged by size: 4611 1114 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2351 2351 Size: 6103 FspI TGC'GCA Cuts at: 4609 4609 Size: 6103 HaeII r_GCGC'y Cuts at: 1678 2057 5250 1678 Size: 379 3193 2531 Fragments arranged by size: 3193 2531 379 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2096 2128 2716 2748 2779 Size: 32 135 32 524 32 588 32 31 Cuts at: 2779 2811 4744 4776 4818 4850 1373 Size: 32 1933 32 42 32 2626 Fragments arranged by size: 2626 1933 588 524 135 42 32 32 32 32 32 32 32 31 HincII GTy'rAC Cuts at: 669 1657 1938 3447 3693 669 Size: 988 281 1509 246 3079 Fragments arranged by size: 3079 1509 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6103 HpaI GTT'AAC Cuts at: 3693 3693 Size: 6103 KpnI G_GTAC'C Cuts at: 3428 3428 Size: 6103 MfeI C'AATT_G Cuts at: 2605 3702 2605 Size: 1097 5006 MluI A'CGCG_T Cuts at: 5979 5979 Size: 6103 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2085 2781 5217 5401 1305 Size: 780 696 2436 184 2007 Fragments arranged by size: 2436 2007 780 696 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2294 2774 10 Size: 54 1425 343 462 480 3339 Fragments arranged by size: 3339 1425 480 462 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2375 4061 4420 4579 518 Size: 648 1209 1686 359 159 2042 Fragments arranged by size: 2042 1686 1209 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2401 3247 3519 4196 5344 5589 1606 Size: 795 846 272 677 1148 245 2120 Fragments arranged by size: 2120 1148 846 795 677 272 245 NcoI C'CATG_G Cuts at: 513 1065 2247 513 Size: 552 1182 4369 Fragments arranged by size: 4369 1182 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6103 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6103 NotI GC'GGCC_GC Cuts at: 3550 3550 Size: 6103 NspI r_CATG'y Cuts at: 1132 1229 2047 2146 3079 3461 5006 1132 Size: 97 818 99 933 382 1545 2229 Fragments arranged by size: 2229 1545 933 818 382 99 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6103 PciI A'CATG_T Cuts at: 2142 3075 5002 2142 Size: 933 1927 3243 Fragments arranged by size: 3243 1927 933 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6103 PmeI GTTT'AAAC Cuts at: 3407 3407 Size: 6103 PmlI CAC'GTG Cuts at: 2341 2341 Size: 6103 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4172 4204 5685 5717 4172 Size: 32 1481 32 4558 Fragments arranged by size: 4558 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6103 PsiI TTA'TAA Cuts at: 3673 3673 Size: 6103 PstI C_TGCA'G Cuts at: 830 2088 2406 2676 2732 3243 3252 3455 830 Size: 1258 318 270 56 511 9 203 3478 Fragments arranged by size: 3478 1258 511 318 270 203 56 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6103 PvuII CAG'CTG Cuts at: 2401 3247 2401 Size: 846 5257 SalI G'TCGA_C Cuts at: 1936 3445 1936 Size: 1509 4594 SapI GCTCTTCn'nnn_ Cuts at: 2235 2235 Size: 6103 SbfI CC_TGCA'GG Cuts at: 3455 3455 Size: 6103 ScaI AGT'ACT Cuts at: 4351 6063 4351 Size: 1712 4391 SfcI C'TryA_G Cuts at: 826 1045 2084 2402 2480 2672 2728 3239 3248 Size: 219 1039 318 78 192 56 511 9 Cuts at: 3248 3451 4586 5267 5458 826 Size: 203 1135 681 191 1471 Fragments arranged by size: 1471 1135 1039 681 511 318 219 203 192 191 78 56 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6103 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6103 SmaI CCC'GGG Cuts at: 1872 3430 1872 Size: 1558 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2012 3332 4207 5108 5370 820 Size: 197 926 69 1320 875 901 262 1553 Fragments arranged by size: 1553 1320 926 901 875 262 197 69 SnaBI TAC'GTA Cuts at: 493 3894 493 Size: 3401 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6103 SphI G_CATG'C Cuts at: 1132 2047 3461 1132 Size: 915 1414 3774 Fragments arranged by size: 3774 1414 915 SspI AAT'ATT Cuts at: 5 52 4027 5 Size: 47 3975 2081 Fragments arranged by size: 3975 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6103 StyI C'CwwG_G Cuts at: 513 1065 2247 2637 2966 3067 3542 513 Size: 552 1182 390 329 101 475 3074 Fragments arranged by size: 3074 1182 552 475 390 329 101 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2150 3370 4130 4147 4300 4485 4930 Size: 1255 570 1220 760 17 153 185 445 Cuts at: 4930 325 Size: 1498 Fragments arranged by size: 1498 1255 1220 760 570 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2145 2754 4349 Size: 274 80 33 51 1443 168 609 1595 Cuts at: 4349 6061 96 Size: 1712 138 Fragments arranged by size: 1712 1595 1443 609 274 168 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2184 2216 6063 6095 2184 Size: 32 3847 32 2192 Fragments arranged by size: 3847 2192 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6103 XbaI T'CTAG_A Cuts at: 3439 3439 Size: 6103 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3046 3046 Size: 6103 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6103 XmaI C'CCGG_G Cuts at: 1870 3428 1870 Size: 1558 4545 XmnI GAAnn'nnTTC Cuts at: 2826 4232 2826 Size: 1406 4697 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3911 276 Size: 53 83 186 936 2377 2468 Fragments arranged by size: 2468 2377 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI