Restriction map for
pFN21AB5309
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanII BbvCI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BseRI
BsiWI BsmAI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssSI Bst1107I BstAPI
BstBI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI Eam1105I
Eco47III Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI MamI MfeI
MluI MluNI MroI MscI MseI
MunI NdeI NheI NotI NsiI
NspV PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SalI
SbfI SfcI SfiI SfuI SgrAI
SnaBI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp509I Tth111I XbaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AscI 884 GGCGCGCC GG^CGCGCC
BbeI 1213 GGCGCC GGCGC^C
BbrPI 549 CACGTG CAC^GTG
BbsI 243 GAAGAC GAAGAC(2/6)
BfaI 68 CTAG C^TAG
BglI 115 GCCGGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 243 GAAGAC GAAGAC(2/6)
BsaAI 549 CACGTG [CT]AC^GT[GA]
BsrDI 335 GCAATG GCAATG(2/0)
BsrI 1194 CCAGT ACTGG(1/-1)
BstEII 1227 GGTCACC G^GT[ACGT]ACC
BstPI 1227 GGTCACC G^GT[ACGT]ACC
BstYI 1190 AGATCC [GA]^GATC[CT]
DdeI 38 CTAAG C^T[ACGT]AG
EcoNI 372 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1227 GGTCACC G^GT[ACGT]ACC
EcoT14I 32 CCATGG C^C[AT][AT]GG
HaeII 1213 GGCGCC [GA]GCGC^[CT]
HphI 1229 TCACC GGTGA(8/7)
KasI 1213 GGCGCC G^GCGCC
MaeI 68 CTAG C^TAG
MaeIII 1228 GTCAC ^GT[ACGT]AC
MflI 1190 AGATCC [GA]^GATC[CT]
MslI 477 CATCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1213 GGCGCC GG^CGCC
NcoI 32 CCATGG C^CATGG
NruI 1251 TCGCGA TCG^CGA
PaeR7I 169 CTCGAG C^TCGAG
PmaCI 549 CACGTG CAC^GTG
PmlI 549 CACGTG CAC^GTG
PpuMI 788 AGGACCT [GA]G^G[AT]CC[CT]
ScaI 758 AGTACT AGT^ACT
SexAI 740 ACCTGGT A^CC[AT]GGT
SfoI 1213 GGCGCC GGC^GCC
SmaI 391 CCCGGG CCC^GGG
SpeI 67 ACTAGT A^CTAGT
StuI 959 AGGCCT AGG^CCT
StyI 32 CCATGG C^C[AT][AT]GG
Tsp45I 1228 GTCAC ^GT[GC]AC
TspRI 182 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 1109 CCAAGTCCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 169 CTCGAG C^TCGAG
XhoII 1190 AGATCC [GA]^GATC[CT]
XmaCI 391 CCCGGG C^CCGGG
XmaI 391 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 1057 GACGCC G[GA]^CG[CT]C
1213 GGCGCC
BcgI 335 GCAATGACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
378 GCAGGACTATCG
BpmI 718 CTGGAG CTGGAG(16/14)
808 CTGGAG
BsaHI 1057 GACGCC G[GA]^CG[CT]C
1213 GGCGCC
BsiEI 330 CGGCCG CG[GA][CT]^CG
563 CGGCCG
BssHII 56 GCGCGC G^CGCGC
885 GCGCGC
DraII 394 GGGGCCT [GA]G^G[ACGT]CC[CT]
788 AGGACCT
DraIII 739 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1048 CACGCCGTG
EagI 330 CGGCCG C^GGCCG
563 CGGCCG
EclXI 330 CGGCCG C^GGCCG
563 CGGCCG
Eco52I 330 CGGCCG C^GGCCG
563 CGGCCG
EcoO109I 394 GGGGCCT [GA]G^G[ACGT]CC[CT]
788 AGGACCT
Hin1I 1057 GACGCC G[GA]^CG[CT]C
1213 GGCGCC
KspI 220 CCGCGG CCGC^GG
859 CCGCGG
MaeII 550 ACGT A^CGT
815 ACGT
SacII 220 CCGCGG CCGC^GG
859 CCGCGG
SfaNI 1034 GCATC GCATC(5/9)
1142 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BbvI BcnI BmyI BsaJI BsgI
BsiHKAI BsiYI BslI BsoBI Bsp1286I
BspMI BsrFI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DpnII DsaI EaeI EarI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
ItaI Ksp632I MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI RsaI
SapI Sau3AI Sau96I ScrFI SmlI
SphI TaqI TseI TthHB8I Van91I