Restriction map for
pFN21AB5309
(Circular) (Six-base) MAPSORT of: pFN21AB5309.seq Check: 9182 from: 1 to: 5974
pFN21AB5309 5974 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2176 2695 2176
Size: 519 5455
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3784 278
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3317 1937
Size: 1380 4594
Acc65I G'GTAC_C
Cuts at: 3295 3295
Size: 5974
AclI AA'CG_TT
Cuts at: 1563 4101 4474 1563
Size: 2538 373 3063
Fragments arranged by size:
3063 2538 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2810 4037 5421 5689 1632
Size: 1178 1227 1384 268 1917
Fragments arranged by size:
1917 1384 1227 1178 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5777
AflIII A'CryG_T
Cuts at: 1203 4873 5850 1203
Size: 3670 977 1327
Fragments arranged by size:
3670 1327 977
AgeI A'CCGG_T
Cuts at: 4805 4805
Size: 5974
AhdI GACnn_n'nnGTC
Cuts at: 4703 4703
Size: 5974
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5974
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2173 2291 2396 2444 1184
Size: 989 118 105 48 4714
Fragments arranged by size:
4714 989 118 105 48
AlwNI CAG_nnn'CTG
Cuts at: 2949 3106 3205 3717 5289 2949
Size: 157 99 512 1572 3634
Fragments arranged by size:
3634 1572 512 157 99
ApaLI G'TGCA_C
Cuts at: 4031 5187 4031
Size: 1156 4818
ApoI r'AATT_y
Cuts at: 1493 3283 3509 3661 5770 1493
Size: 1790 226 152 2109 1697
Fragments arranged by size:
2109 1790 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2890 2890
Size: 5974
AseI AT'TA_AT
Cuts at: 160 1032 4528 160
Size: 872 3496 1606
Fragments arranged by size:
3496 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
AvaI C'yCGr_G
Cuts at: 1870 1943 2174 2396 2872 3191 3299 1870
Size: 73 231 222 476 319 108 4545
Fragments arranged by size:
4545 476 319 231 222 108 73
BamHI G'GATC_C
Cuts at: 3304 3671 3304
Size: 367 5607
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2461 2545 3110 3218 3295
Size: 325 217 627 674 84 565 108 77
Cuts at: 3295 4750 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 674 627 565 325 217 108
84 77
BanII G_rGCy'C
Cuts at: 1391 3293 1391
Size: 1902 4072
BbeI G_GCGC'C
Cuts at: 3222 3222
Size: 5974
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2255 928
Size: 971 356 4647
Fragments arranged by size:
4647 971 356
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2329 2363 2372 2406 4165
Size: 34 50 34 308 34 9 34 1759
Cuts at: 4165 4199 1903
Size: 34 3678
Fragments arranged by size:
3678 1759 308 50 34 34 34 34
34 9
BciVI GTATCCnnnnn_n'
Cuts at: 3866 5076 3866
Size: 1210 4764
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5974
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2126 4585 136
Size: 107 122 71 631 605 454 2459 1525
Fragments arranged by size:
2459 1525 631 605 454 122 107 71
BglII A'GATC_T
Cuts at: 5969 5969
Size: 5974
BlpI GC'TnA_GC
Cuts at: 3391 3391
Size: 5974
Bme1580I G_kGCm'C
Cuts at: 1790 2109 2550 3113 4035 5191 1790
Size: 319 441 563 922 1156 2573
Fragments arranged by size:
2573 1156 922 563 441 319
BmgBI CAC'GTC
Cuts at: 1318 2821 1318
Size: 1503 4471
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3705 4663 442
Size: 416 53 83 399 24 2288 958 1753
Fragments arranged by size:
2288 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2744 2834 4634 1239
Size: 1505 90 1800 2579
Fragments arranged by size:
2579 1800 1505 90
Bpu10I CC'TnA_GC
Cuts at: 2043 2043
Size: 5974
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2300 3122 4099 4964 5262 2300
Size: 822 977 865 298 3012
Fragments arranged by size:
3012 977 865 822 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4637 882
Size: 617 3138 2219
Fragments arranged by size:
3138 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2556 3765 493
Size: 711 1352 1209 2702
Fragments arranged by size:
2702 1352 1209 711
BsaBI GATnn'nnATC
Cuts at: 5968 5968
Size: 5974
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3063 3219 3781 4163
Size: 53 83 186 936 1530 156 562 382
Cuts at: 4163 4798 275
Size: 635 1451
Fragments arranged by size:
1530 1451 936 635 562 382 186 156
83 53
BsaWI w'CCGG_w
Cuts at: 4407 4805 5079 5226 5637 4407
Size: 398 274 147 411 4744
Fragments arranged by size:
4744 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5749
Fragments arranged by size:
5749 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5974
BseYI C'CCAG_C
Cuts at: 2056 2693 2805 3106 5177 5673 2056
Size: 637 112 301 2071 496 2357
Fragments arranged by size:
2357 2071 637 496 301 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2154 2402 2729 1163
Size: 573 418 248 327 4408
Fragments arranged by size:
4408 573 418 327 248
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2338 2571 3424 4185 5213 1540
Size: 275 186 337 233 853 761 1028 2301
Fragments arranged by size:
2301 1028 853 761 337 275 233 186
BsiHKAI G_wGCw'C
Cuts at: 2493 2718 2754 2826 4035 4120 5191 2493
Size: 225 36 72 1209 85 1071 3276
Fragments arranged by size:
3276 1209 1071 225 85 72 36
BsmI GAATG_Cn'
Cuts at: 3485 3578 3485
Size: 93 5881
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2493 2550 2718 2754 2826 3113
Size: 399 319 384 57 168 36 72 287
Cuts at: 3113 3293 4035 4120 5191 1391
Size: 180 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 384 319 287 180
168 85 72 57 36
BspEI T'CCGG_A
Cuts at: 5637 5637
Size: 5974
BspHI T'CATG_A
Cuts at: 3863 5593 3863
Size: 1730 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2176 2661 2695 2805 3329 844
Size: 1332 485 34 110 524 3489
Fragments arranged by size:
3489 1332 524 485 110 34
BsrBI CCG'CTC
Cuts at: 1394 1823 3861 1394
Size: 429 2038 3507
Fragments arranged by size:
3507 2038 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2347 4469 4643 65
Size: 1183 1099 2122 174 1396
Fragments arranged by size:
2122 1396 1183 1099 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2120 2310 2539 2570 3174 4618 4805
Size: 110 310 190 229 31 604 1444 187
Cuts at: 4805 1700
Size: 2869
Fragments arranged by size:
2869 1444 604 310 229 190 187 110
31
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5974
BssHII G'CGCG_C
Cuts at: 1925 2061 2890 1925
Size: 136 829 5009
Fragments arranged by size:
5009 829 136
BssSI C'ACGA_G
Cuts at: 4034 5046 4034
Size: 1012 4962
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5974
BstBI TT'CG_AA
Cuts at: 4778 4778
Size: 5974
BstEII G'GTnAC_C
Cuts at: 1184 3232 1184
Size: 2048 3926
BstYI r'GATC_y
Cuts at: 1972 3195 3304 3671 4056 4073 5525 5611 5623
Size: 1223 109 367 385 17 1452 86 12
Cuts at: 5623 5969 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1223 385 367 346 109 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2037 2225 2864 3057 513
Size: 552 972 188 639 193 3430
Fragments arranged by size:
3430 972 639 552 193 188
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5235
Fragments arranged by size:
5235 683 56
BtsI GCAGTG_nn'
Cuts at: 2193 3490 4284 4304 2193
Size: 1297 794 20 3863
Fragments arranged by size:
3863 1297 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5939
DraI TTT'AAA
Cuts at: 3278 3278
Size: 5974
DraIII CAC_nnn'GTG
Cuts at: 2749 3058 2749
Size: 309 5665
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4981 809
Size: 507 3665 1802
Fragments arranged by size:
3665 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2262 2299 2335
Size: 54 1425 50 275 18 432 37 36
Cuts at: 2335 2568 2997 3421 4310 5939 8
Size: 233 429 424 889 1629 43
Fragments arranged by size:
1629 1425 889 432 429 424 275 233
54 50 43 37 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 2335 2568 3421 1537
Size: 275 523 233 853 4090
Fragments arranged by size:
4090 853 523 275 233
EarI CTCTTCn'nnn_
Cuts at: 1353 2072 2663 3137 3904 1353
Size: 719 591 474 767 3423
Fragments arranged by size:
3423 767 719 591 474
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4561 4935 5081 387
Size: 1064 138 2972 374 146 1280
Fragments arranged by size:
2972 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2744 2810 2834 4037 4634 5421 5689
Size: 393 1112 66 24 1203 597 787 268
Cuts at: 5689 1239
Size: 1524
Fragments arranged by size:
1524 1203 1112 787 597 393 268 66
24
EcoNI CCTnn'n_nnAGG
Cuts at: 2381 2381
Size: 5974
EcoO109I rG'GnC_Cy
Cuts at: 2400 2794 2400
Size: 394 5580
EcoRI G'AATT_C
Cuts at: 3283 3661 3283
Size: 378 5596
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2341 2341
Size: 5974
FspI TGC'GCA
Cuts at: 4480 4480
Size: 5974
HaeII r_GCGC'y
Cuts at: 1678 3222 5121 1678
Size: 1544 1899 2531
Fragments arranged by size:
2531 1899 1544
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4615 4647 4689 4721 1373
Size: 32 135 32 3043 32 42 32 2626
Fragments arranged by size:
3043 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3318 3564 669
Size: 988 281 1380 246 3079
Fragments arranged by size:
3079 1380 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5974
HpaI GTT'AAC
Cuts at: 3564 3564
Size: 5974
KasI G'GCGC_C
Cuts at: 3218 3218
Size: 5974
KpnI G_GTAC'C
Cuts at: 3299 3299
Size: 5974
MfeI C'AATT_G
Cuts at: 3573 3573
Size: 5974
MluI A'CGCG_T
Cuts at: 5850 5850
Size: 5974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5088 5272 1305
Size: 3783 184 2007
Fragments arranged by size:
3783 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4152
Fragments arranged by size:
4152 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2486 3932 4291 4450 518
Size: 648 1320 1446 359 159 2042
Fragments arranged by size:
2042 1446 1320 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2113 2227 2866 3106 3157 3390 4067 5215
Size: 507 114 639 240 51 233 677 1148
Cuts at: 5215 5460 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 639 507 245 240 233
114 51
NaeI GCC'GGC
Cuts at: 2122 2572 3176 2122
Size: 450 604 4920
Fragments arranged by size:
4920 604 450
NarI GG'CG_CC
Cuts at: 3219 3219
Size: 5974
NcoI C'CATG_G
Cuts at: 513 1065 2037 513
Size: 552 972 4450
Fragments arranged by size:
4450 972 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5974
NgoMIV G'CCGG_C
Cuts at: 2120 2570 3174 2120
Size: 450 604 4920
Fragments arranged by size:
4920 604 450
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5974
NotI GC'GGCC_GC
Cuts at: 3421 3421
Size: 5974
NruI TCG'CGA
Cuts at: 3258 3258
Size: 5974
NspI r_CATG'y
Cuts at: 1132 1229 2120 2431 2911 3028 3184 3332 4877
Size: 97 891 311 480 117 156 148 1545
Cuts at: 4877 1132
Size: 2229
Fragments arranged by size:
2229 1545 891 480 311 156 148 117
97
PasI CC'CwG_GG
Cuts at: 1348 2028 1348
Size: 680 5294
PciI A'CATG_T
Cuts at: 4873 4873
Size: 5974
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2901 2949 3058 3228 3243 1108
Size: 1793 48 109 170 15 3839
Fragments arranged by size:
3839 1793 170 109 48 15
PfoI T'CCnGG_A
Cuts at: 2721 3075 2721
Size: 354 5620
PmeI GTTT'AAAC
Cuts at: 3278 3278
Size: 5974
PmlI CAC'GTG
Cuts at: 2556 2556
Size: 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4043 4075 5556 5588 4043
Size: 32 1481 32 4429
Fragments arranged by size:
4429 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2794 2794
Size: 5974
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5974
PsiI TTA'TAA
Cuts at: 3544 3544
Size: 5974
PspXI vC'TCGA_Gb
Cuts at: 2174 2174
Size: 5974
PstI C_TGCA'G
Cuts at: 830 3326 830
Size: 2496 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5974
SacII CC_GC'GG
Cuts at: 2228 2867 2228
Size: 639 5335
SalI G'TCGA_C
Cuts at: 1936 3316 1936
Size: 1380 4594
SapI GCTCTTCn'nnn_
Cuts at: 2072 2663 3137 2072
Size: 591 474 4909
Fragments arranged by size:
4909 591 474
SbfI CC_TGCA'GG
Cuts at: 3326 3326
Size: 5974
ScaI AGT'ACT
Cuts at: 2765 4222 5934 2765
Size: 1457 1712 2805
Fragments arranged by size:
2805 1712 1457
SexAI A'CCwGG_T
Cuts at: 2745 2745
Size: 5974
SfcI C'TryA_G
Cuts at: 826 1045 3322 4457 5138 5329 826
Size: 219 2277 1135 681 191 1471
Fragments arranged by size:
2277 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 3220 3220
Size: 5974
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5974
SmaI CCC'GGG
Cuts at: 1872 2398 3301 1872
Size: 526 903 4545
Fragments arranged by size:
4545 903 526
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2174 2315 3137 4078 4979 5241
Size: 197 926 231 141 822 941 901 262
Cuts at: 5241 820
Size: 1553
Fragments arranged by size:
1553 941 926 901 822 262 231 197
141
SnaBI TAC'GTA
Cuts at: 493 3765 493
Size: 3272 2702
SpeI A'CTAG_T
Cuts at: 152 2072 152
Size: 1920 4054
SphI G_CATG'C
Cuts at: 1132 2120 3028 3184 3332 1132
Size: 988 908 156 148 3774
Fragments arranged by size:
3774 988 908 156 148
SspI AAT'ATT
Cuts at: 5 52 3898 5
Size: 47 3846 2081
Fragments arranged by size:
3846 2081 47
StuI AGG'CCT
Cuts at: 1517 2966 1517
Size: 1449 4525
StyI C'CwwG_G
Cuts at: 513 1065 2037 3413 513
Size: 552 972 1376 3074
Fragments arranged by size:
3074 1376 972 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2600 4001 4018 4171 4356 4801 325
Size: 1255 1020 1401 17 153 185 445 1498
Fragments arranged by size:
1498 1401 1255 1020 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2091 2763 4220
Size: 274 80 33 51 1443 114 672 1457
Cuts at: 4220 5932 96
Size: 1712 138
Fragments arranged by size:
1712 1457 1443 672 274 138 114 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2786 2818 5934 5966 2786
Size: 32 3116 32 2794
Fragments arranged by size:
3116 2794 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5974
XbaI T'CTAG_A
Cuts at: 3310 3310
Size: 5974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3121 3121
Size: 5974
XhoI C'TCGA_G
Cuts at: 1943 2174 1943
Size: 231 5743
XmaI C'CCGG_G
Cuts at: 1870 2396 3299 1870
Size: 526 903 4545
Fragments arranged by size:
4545 903 526
XmnI GAAnn'nnTTC
Cuts at: 4103 4103
Size: 5974
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3782 276
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI
FspAI NsiI PacI PspOMI PsrI PvuII RsrII SacI
SanDI SfiI SrfI SwaI