Restriction map for
pFN21AB5309
(Linear) (Six-base) MAPSORT of: KIBB5309.seq Check: 29 from: 1 to: 1269
KIBB5309.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 171 690 1269
Size: 171 519 579
Fragments arranged by size:
579 519 171
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 805 1269
Size: 805 464
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 168 286 391 439 1269
Size: 168 118 105 48 830
Fragments arranged by size:
830 168 118 105 48
AlwNI CAG_nnn'CTG
Cuts at: 0 944 1101 1200 1269
Size: 944 157 99 69
Fragments arranged by size:
944 157 99 69
AscI GG'CGCG_CC
Cuts at: 0 885 1269
Size: 885 384
AvaI C'yCGr_G
Cuts at: 0 169 391 867 1186 1269
Size: 169 222 476 319 83
Fragments arranged by size:
476 319 222 169 83
BanI G'GyrC_C
Cuts at: 0 456 540 1105 1213 1269
Size: 456 84 565 108 56
Fragments arranged by size:
565 456 108 84 56
BbeI G_GCGC'C
Cuts at: 0 1217 1269
Size: 1217 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 250 1269
Size: 250 1019
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 324 358 367 401 1269
Size: 324 34 9 34 868
Fragments arranged by size:
868 324 34 34 9
BglI GCCn_nnn'nGGC
Cuts at: 0 121 1269
Size: 121 1148
Bme1580I G_kGCm'C
Cuts at: 0 104 545 1108 1269
Size: 104 441 563 161
Fragments arranged by size:
563 441 161 104
BmgBI CAC'GTC
Cuts at: 0 816 1269
Size: 816 453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 739 829 1269
Size: 739 90 440
Fragments arranged by size:
739 440 90
Bpu10I CC'TnA_GC
Cuts at: 0 38 1269
Size: 38 1231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1117 1269
Size: 295 822 152
Fragments arranged by size:
822 295 152
BsaAI yAC'GTr
Cuts at: 0 551 1269
Size: 551 718
BsaHI Gr'CG_yC
Cuts at: 0 1058 1214 1269
Size: 1058 156 55
Fragments arranged by size:
1058 156 55
BseYI C'CCAG_C
Cuts at: 0 51 688 800 1101 1269
Size: 51 637 112 301 168
Fragments arranged by size:
637 301 168 112 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 397 724 1269
Size: 149 248 327 545
Fragments arranged by size:
545 327 248 149
BsiEI CG_ry'CG
Cuts at: 0 333 566 1269
Size: 333 233 703
Fragments arranged by size:
703 333 233
BsiHKAI G_wGCw'C
Cuts at: 0 488 713 749 821 1269
Size: 488 225 36 72 448
Fragments arranged by size:
488 448 225 72 36
Bsp1286I G_dGCh'C
Cuts at: 0 104 488 545 713 749 821 1108 1269
Size: 104 384 57 168 36 72 287 161
Fragments arranged by size:
384 287 168 161 104 72 57 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 171 656 690 800 1269
Size: 171 485 34 110 469
Fragments arranged by size:
485 469 171 110 34
BsrDI GCAATG_nn'
Cuts at: 0 342 1269
Size: 342 927
BsrFI r'CCGG_y
Cuts at: 0 115 305 534 565 1169 1269
Size: 115 190 229 31 604 100
Fragments arranged by size:
604 229 190 115 100 31
BssHII G'CGCG_C
Cuts at: 0 56 885 1269
Size: 56 829 384
Fragments arranged by size:
829 384 56
BstEII G'GTnAC_C
Cuts at: 0 1227 1269
Size: 1227 42
BstYI r'GATC_y
Cuts at: 0 1190 1269
Size: 1190 79
BtgI C'CryG_G
Cuts at: 0 32 220 859 1052 1269
Size: 32 188 639 193 217
Fragments arranged by size:
639 217 193 188 32
BtsI GCAGTG_nn'
Cuts at: 0 188 1269
Size: 188 1081
DraIII CAC_nnn'GTG
Cuts at: 0 744 1053 1269
Size: 744 309 216
Fragments arranged by size:
744 309 216
EaeI y'GGCC_r
Cuts at: 0 257 294 330 563 992 1269
Size: 257 37 36 233 429 277
Fragments arranged by size:
429 277 257 233 37 36
EagI C'GGCC_G
Cuts at: 0 330 563 1269
Size: 330 233 706
Fragments arranged by size:
706 330 233
EarI CTCTTCn'nnn_
Cuts at: 0 67 658 1132 1269
Size: 67 591 474 137
Fragments arranged by size:
591 474 137 67
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 739 805 829 1269
Size: 739 66 24 440
Fragments arranged by size:
739 440 66 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 376 1269
Size: 376 893
EcoO109I rG'GnC_Cy
Cuts at: 0 395 789 1269
Size: 395 394 480
Fragments arranged by size:
480 395 394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 336 1269
Size: 336 933
HaeII r_GCGC'y
Cuts at: 0 1217 1269
Size: 1217 52
KasI G'GCGC_C
Cuts at: 0 1213 1269
Size: 1213 56
MslI CAynn'nnrTG
Cuts at: 0 481 1269
Size: 481 788
MspA1I CmG'CkG
Cuts at: 0 108 222 861 1101 1152 1269
Size: 108 114 639 240 51 117
Fragments arranged by size:
639 240 117 114 108 51
NaeI GCC'GGC
Cuts at: 0 117 567 1171 1269
Size: 117 450 604 98
Fragments arranged by size:
604 450 117 98
NarI GG'CG_CC
Cuts at: 0 1214 1269
Size: 1214 55
NcoI C'CATG_G
Cuts at: 0 32 1269
Size: 32 1237
NgoMIV G'CCGG_C
Cuts at: 0 115 565 1169 1269
Size: 115 450 604 100
Fragments arranged by size:
604 450 115 100
NruI TCG'CGA
Cuts at: 0 1253 1269
Size: 1253 16
NspI r_CATG'y
Cuts at: 0 115 426 906 1023 1179 1269
Size: 115 311 480 117 156 90
Fragments arranged by size:
480 311 156 117 115 90
PasI CC'CwG_GG
Cuts at: 0 23 1269
Size: 23 1246
PflMI CCAn_nnn'nTGG
Cuts at: 0 896 944 1053 1223 1238 1269
Size: 896 48 109 170 15 31
Fragments arranged by size:
896 170 109 48 31 15
PfoI T'CCnGG_A
Cuts at: 0 716 1070 1269
Size: 716 354 199
Fragments arranged by size:
716 354 199
PmlI CAC'GTG
Cuts at: 0 551 1269
Size: 551 718
PpuMI rG'GwC_Cy
Cuts at: 0 789 1269
Size: 789 480
PspXI vC'TCGA_Gb
Cuts at: 0 169 1269
Size: 169 1100
SacII CC_GC'GG
Cuts at: 0 223 862 1269
Size: 223 639 407
Fragments arranged by size:
639 407 223
SapI GCTCTTCn'nnn_
Cuts at: 0 67 658 1132 1269
Size: 67 591 474 137
Fragments arranged by size:
591 474 137 67
ScaI AGT'ACT
Cuts at: 0 760 1269
Size: 760 509
SexAI A'CCwGG_T
Cuts at: 0 740 1269
Size: 740 529
SfoI GGC'GCC
Cuts at: 0 1215 1269
Size: 1215 54
SmaI CCC'GGG
Cuts at: 0 393 1269
Size: 393 876
SmlI C'TyrA_G
Cuts at: 0 169 310 1132 1269
Size: 169 141 822 137
Fragments arranged by size:
822 169 141 137
SpeI A'CTAG_T
Cuts at: 0 67 1269
Size: 67 1202
SphI G_CATG'C
Cuts at: 0 115 1023 1179 1269
Size: 115 908 156 90
Fragments arranged by size:
908 156 115 90
StuI AGG'CCT
Cuts at: 0 961 1269
Size: 961 308
StyI C'CwwG_G
Cuts at: 0 32 1269
Size: 32 1237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 595 1269
Size: 595 674
TatI w'GTAC_w
Cuts at: 0 86 758 1269
Size: 86 672 511
Fragments arranged by size:
672 511 86
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 781 813 1269
Size: 781 32 456
Fragments arranged by size:
781 456 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1116 1269
Size: 1116 153
XhoI C'TCGA_G
Cuts at: 0 169 1269
Size: 169 1100
XmaI C'CCGG_G
Cuts at: 0 391 1269
Size: 391 878
Enzymes that do cut:
AarI AcuI AlfI AlwNI AscI AvaI BanI BbeI
BbsI BcgI BglI Bme1580I BmgBI BpmI Bpu10I BpuEI
BsaAI BsaHI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrDI BsrFI BssHII BstEII BstYI BtgI BtsI DraIII
EaeI EagI EarI Eco57MI EcoNI EcoO109I FalI HaeII
KasI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PasI PflMI PfoI PmlI PpuMI PspXI SacII
SapI ScaI SexAI SfoI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI ApoI AseI AsiSI
AvrII BaeI BamHI BanII BbvCI BciVI BclI BfrBI
BglII BlpI BmrI BmtI BplI BsaI BsaBI BsaWI
BsaXI BseRI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrGI BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EciI EcoICRI EcoRI EcoRV
FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI NdeI NheI NotI NsiI
PacI PciI PmeI PpiI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SbfI
SfcI SfiI SgfI SgrAI SnaBI SrfI SspI SwaI
Tth111I XbaI XmnI ZraI