(Circular) (Six-base) MAPSORT of: pFN21AB5220.seq Check: 3684 from: 1 to: 6811 pFN21AB5220 6811 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4621 278 Size: 53 83 186 936 3085 2468 Fragments arranged by size: 3085 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4154 1937 Size: 2217 4594 Acc65I G'GTAC_C Cuts at: 4132 4132 Size: 6811 AclI AA'CG_TT Cuts at: 1563 4938 5311 1563 Size: 3375 373 3063 Fragments arranged by size: 3375 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2906 3758 4874 6258 6526 1632 Size: 1274 852 1116 1384 268 1917 Fragments arranged by size: 1917 1384 1274 1116 852 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6614 AflIII A'CryG_T Cuts at: 1203 3963 5710 6687 1203 Size: 2760 1747 977 1327 Fragments arranged by size: 2760 1747 1327 977 AgeI A'CCGG_T Cuts at: 5642 5642 Size: 6811 AhdI GACnn_n'nnGTC Cuts at: 5540 5540 Size: 6811 AleI CACnn'nnGTG Cuts at: 1166 2885 1166 Size: 1719 5092 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2903 1184 Size: 1719 5092 AlwNI CAG_nnn'CTG Cuts at: 3152 3920 4554 6126 3152 Size: 768 634 1572 3837 Fragments arranged by size: 3837 1572 768 634 ApaLI G'TGCA_C Cuts at: 4868 6024 4868 Size: 1156 5655 ApoI r'AATT_y Cuts at: 1493 2941 4120 4346 4498 6607 1493 Size: 1448 1179 226 152 2109 1697 Fragments arranged by size: 2109 1697 1448 1179 226 152 AseI AT'TA_AT Cuts at: 160 1032 3057 5365 160 Size: 872 2025 2308 1606 Fragments arranged by size: 2308 2025 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6811 AvaI C'yCGr_G Cuts at: 1870 1943 3804 4136 1870 Size: 73 1861 332 4545 Fragments arranged by size: 4545 1861 332 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3900 3933 3900 Size: 33 6778 BamHI G'GATC_C Cuts at: 4141 4508 4141 Size: 367 6444 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2064 4132 5587 618 Size: 325 217 627 277 2068 1455 1842 Fragments arranged by size: 2068 1842 1455 627 325 277 217 BanII G_rGCy'C Cuts at: 1391 4130 1391 Size: 2739 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2909 3855 928 Size: 971 1010 946 3884 Fragments arranged by size: 3884 1010 971 946 BbvCI CC'TCA_GC Cuts at: 3483 3483 Size: 6811 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5002 5036 1903 Size: 34 50 34 2981 34 3678 Fragments arranged by size: 3678 2981 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4703 5913 4703 Size: 1210 5601 BclI T'GATC_A Cuts at: 1548 1548 Size: 6811 BfrBI ATG'CAT Cuts at: 2722 2722 Size: 6811 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5422 136 Size: 107 122 71 631 605 3750 1525 Fragments arranged by size: 3750 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2752 3382 6806 2752 Size: 630 3424 2757 Fragments arranged by size: 3424 2757 630 BlpI GC'TnA_GC Cuts at: 4228 4228 Size: 6811 Bme1580I G_kGCm'C Cuts at: 1790 4872 6028 1790 Size: 3082 1156 2573 Fragments arranged by size: 3082 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6811 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3908 4542 5500 Size: 416 53 83 399 24 2491 634 958 Cuts at: 5500 442 Size: 1753 Fragments arranged by size: 2491 1753 958 634 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6811 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2805 3758 5471 1239 Size: 1566 953 1713 2579 Fragments arranged by size: 2579 1713 1566 953 Bpu10I CC'TnA_GC Cuts at: 3483 3483 Size: 6811 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3587 4936 5801 6099 3587 Size: 1349 865 298 4299 Fragments arranged by size: 4299 1349 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5474 882 Size: 617 3975 2219 Fragments arranged by size: 3975 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3964 4602 493 Size: 711 2760 638 2702 Fragments arranged by size: 2760 2702 711 638 BsaBI GATnn'nnATC Cuts at: 6805 6805 Size: 6811 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4618 5000 5635 275 Size: 53 83 186 936 3085 382 635 1451 Fragments arranged by size: 3085 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2309 5244 5642 5916 6063 6474 2309 Size: 2935 398 274 147 411 2646 Fragments arranged by size: 2935 2646 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6586 Fragments arranged by size: 6586 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3311 3998 1186 Size: 2125 687 3999 Fragments arranged by size: 3999 2125 687 BseYI C'CCAG_C Cuts at: 6014 6510 6014 Size: 496 6315 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6238 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4261 5022 6050 1540 Size: 275 186 2260 761 1028 2301 Fragments arranged by size: 2301 2260 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4872 4957 6028 4872 Size: 85 1071 5655 Fragments arranged by size: 5655 1071 85 BsmI GAATG_Cn' Cuts at: 2696 4322 4415 2696 Size: 1626 93 5092 Fragments arranged by size: 5092 1626 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4130 4872 4957 6028 1391 Size: 399 2340 742 85 1071 2174 Fragments arranged by size: 2340 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6474 6474 Size: 6811 BspHI T'CATG_A Cuts at: 4700 6430 4700 Size: 1730 5081 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4099 4166 844 Size: 3255 67 3489 Fragments arranged by size: 3489 3255 67 BsrBI CCG'CTC Cuts at: 1394 1823 4698 1394 Size: 429 2875 3507 Fragments arranged by size: 3507 2875 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5306 5480 65 Size: 1183 4058 174 1396 Fragments arranged by size: 4058 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2067 5455 5642 1700 Size: 110 257 3388 187 2869 Fragments arranged by size: 3388 2869 257 187 110 BsrGI T'GTAC_A Cuts at: 96 2146 96 Size: 2050 4761 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6811 BssSI C'ACGA_G Cuts at: 4871 5883 4871 Size: 1012 5799 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6811 BstBI TT'CG_AA Cuts at: 5615 5615 Size: 6811 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6811 BstYI r'GATC_y Cuts at: 1972 2752 3064 3382 3937 4141 4508 4893 4910 Size: 780 312 318 555 204 367 385 17 Cuts at: 4910 6362 6448 6460 6806 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 780 555 385 367 346 318 312 204 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2932 2932 Size: 6811 BtgI C'CryG_G Cuts at: 513 1065 2331 513 Size: 552 1266 4993 Fragments arranged by size: 4993 1266 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6072 Fragments arranged by size: 6072 683 56 BtsI GCAGTG_nn' Cuts at: 2701 4327 5121 5141 2701 Size: 1626 794 20 4371 Fragments arranged by size: 4371 1626 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6811 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6776 DraI TTT'AAA Cuts at: 4115 4115 Size: 6811 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5818 809 Size: 507 4502 1802 Fragments arranged by size: 4502 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3474 4258 5147 Size: 54 1425 50 275 18 1644 784 889 Cuts at: 5147 6776 8 Size: 1629 43 Fragments arranged by size: 1644 1629 1425 889 784 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4258 1537 Size: 275 2446 4090 Fragments arranged by size: 4090 2446 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3180 4741 1353 Size: 1827 1561 3423 Fragments arranged by size: 3423 1827 1561 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5398 5772 5918 387 Size: 1064 138 3809 374 146 1280 Fragments arranged by size: 3809 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2805 2906 3758 4874 5471 6258 6526 Size: 393 1173 101 852 1116 597 787 268 Cuts at: 6526 1239 Size: 1524 Fragments arranged by size: 1524 1173 1116 852 787 597 393 268 101 EcoRI G'AATT_C Cuts at: 4120 4498 4120 Size: 378 6433 EcoRV GAT'ATC Cuts at: 3157 3157 Size: 6811 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3404 3515 3404 Size: 111 6700 FspI TGC'GCA Cuts at: 5317 5317 Size: 6811 HaeII r_GCGC'y Cuts at: 1678 2803 5958 1678 Size: 1125 3155 2531 Fragments arranged by size: 3155 2531 1125 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2529 2561 3395 3427 3682 Size: 32 135 32 957 32 834 32 255 Cuts at: 3682 3714 3979 4011 5452 5484 5526 5558 1373 Size: 32 265 32 1441 32 42 32 2626 Fragments arranged by size: 2626 1441 957 834 265 255 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3466 4155 4401 669 Size: 988 281 1528 689 246 3079 Fragments arranged by size: 3079 1528 988 689 281 246 HindIII A'AGCT_T Cuts at: 748 3122 748 Size: 2374 4437 HpaI GTT'AAC Cuts at: 4401 4401 Size: 6811 KpnI G_GTAC'C Cuts at: 4136 4136 Size: 6811 MfeI C'AATT_G Cuts at: 4410 4410 Size: 6811 MluI A'CGCG_T Cuts at: 6687 6687 Size: 6811 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3178 3727 5925 6109 1305 Size: 1873 549 2198 184 2007 Fragments arranged by size: 2198 2007 1873 549 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3476 10 Size: 54 1425 343 1644 3345 Fragments arranged by size: 3345 1644 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2885 3843 4769 5128 5287 518 Size: 648 1719 958 926 359 159 2042 Fragments arranged by size: 2042 1719 958 926 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4227 4904 6052 6297 1606 Size: 2621 677 1148 245 2120 Fragments arranged by size: 2621 2120 1148 677 245 NaeI GCC'GGC Cuts at: 2069 2069 Size: 6811 NcoI C'CATG_G Cuts at: 513 1065 2331 513 Size: 552 1266 4993 Fragments arranged by size: 4993 1266 552 NdeI CA'TA_TG Cuts at: 387 2299 387 Size: 1912 4899 NgoMIV G'CCGG_C Cuts at: 2067 2067 Size: 6811 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6811 NotI GC'GGCC_GC Cuts at: 4258 4258 Size: 6811 NsiI A_TGCA'T Cuts at: 2724 2724 Size: 6811 NspI r_CATG'y Cuts at: 1132 1229 2722 2726 4169 5714 1132 Size: 97 1493 4 1443 1545 2229 Fragments arranged by size: 2229 1545 1493 1443 97 4 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6811 PciI A'CATG_T Cuts at: 5710 5710 Size: 6811 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6811 PmeI GTTT'AAAC Cuts at: 4115 4115 Size: 6811 PmlI CAC'GTG Cuts at: 3964 3964 Size: 6811 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4880 4912 6393 6425 4880 Size: 32 1481 32 5266 Fragments arranged by size: 5266 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6811 PsiI TTA'TAA Cuts at: 2235 4381 2235 Size: 2146 4665 PstI C_TGCA'G Cuts at: 830 4110 4163 830 Size: 3280 53 3478 Fragments arranged by size: 3478 3280 53 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6811 SalI G'TCGA_C Cuts at: 1936 4153 1936 Size: 2217 4594 SbfI CC_TGCA'GG Cuts at: 4163 4163 Size: 6811 ScaI AGT'ACT Cuts at: 5059 6771 5059 Size: 1712 5099 SfcI C'TryA_G Cuts at: 826 1045 2685 4106 4159 5294 5975 6166 826 Size: 219 1640 1421 53 1135 681 191 1471 Fragments arranged by size: 1640 1471 1421 1135 681 219 191 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6811 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6811 SmaI CCC'GGG Cuts at: 1872 4138 1872 Size: 2266 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3566 4915 5816 6078 820 Size: 197 926 1623 1349 901 262 1553 Fragments arranged by size: 1623 1553 1349 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4602 493 Size: 4109 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6811 SphI G_CATG'C Cuts at: 1132 4169 1132 Size: 3037 3774 SspI AAT'ATT Cuts at: 5 52 2968 3037 4735 5 Size: 47 2916 69 1698 2081 Fragments arranged by size: 2916 2081 1698 69 47 StuI AGG'CCT Cuts at: 1517 3732 1517 Size: 2215 4596 StyI C'CwwG_G Cuts at: 513 1065 2331 2842 2862 4250 513 Size: 552 1266 511 20 1388 3074 Fragments arranged by size: 3074 1388 1266 552 511 20 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4838 4855 5008 5193 5638 325 Size: 1255 3258 17 153 185 445 1498 Fragments arranged by size: 3258 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2146 2266 3005 Size: 274 80 33 51 1443 169 120 739 Cuts at: 3005 5057 6769 96 Size: 2052 1712 138 Fragments arranged by size: 2052 1712 1443 739 274 169 138 120 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6771 6803 6771 Size: 32 6779 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6811 XbaI T'CTAG_A Cuts at: 4147 4147 Size: 6811 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6811 XmaI C'CCGG_G Cuts at: 1870 4136 1870 Size: 2266 4545 XmnI GAAnn'nnTTC Cuts at: 2340 2472 4069 4940 2340 Size: 132 1597 871 4211 Fragments arranged by size: 4211 1597 871 132 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4619 276 Size: 53 83 186 936 3085 2468 Fragments arranged by size: 3085 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NarI NruI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI