(Circular) (Six-base) MAPSORT of: pFN21AB5211.seq Check: 1121 from: 1 to: 5839 pFN21AB5211 5839 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2309 2309 Size: 5839 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2406 2924 3649 278 Size: 53 83 186 936 870 518 725 2468 Fragments arranged by size: 2468 936 870 725 518 186 83 53 AccI GT'mk_AC Cuts at: 1937 3182 1937 Size: 1245 4594 Acc65I G'GTAC_C Cuts at: 3160 3160 Size: 5839 AclI AA'CG_TT Cuts at: 1563 2427 3966 4339 1563 Size: 864 1539 373 3063 Fragments arranged by size: 3063 1539 864 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2708 3135 3902 5286 5554 1632 Size: 1076 427 767 1384 268 1917 Fragments arranged by size: 1917 1384 1076 767 427 268 AfeI AGC'GCT Cuts at: 2873 2873 Size: 5839 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5642 AflIII A'CryG_T Cuts at: 1203 2486 2906 4738 5715 1203 Size: 1283 420 1832 977 1327 Fragments arranged by size: 1832 1327 1283 977 420 AgeI A'CCGG_T Cuts at: 4670 4670 Size: 5839 AhdI GACnn_n'nnGTC Cuts at: 4568 4568 Size: 5839 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5839 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5839 AlwNI CAG_nnn'CTG Cuts at: 3582 5154 3582 Size: 1572 4267 ApaI G_GGCC'C Cuts at: 2395 2395 Size: 5839 ApaLI G'TGCA_C Cuts at: 3896 5052 3896 Size: 1156 4683 ApoI r'AATT_y Cuts at: 1493 2708 2843 3148 3374 3526 5635 1493 Size: 1215 135 305 226 152 2109 1697 Fragments arranged by size: 2109 1697 1215 305 226 152 135 AseI AT'TA_AT Cuts at: 160 1032 4393 160 Size: 872 3361 1606 Fragments arranged by size: 3361 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5839 AvaI C'yCGr_G Cuts at: 1870 1943 2386 2826 3164 1870 Size: 73 443 440 338 4545 Fragments arranged by size: 4545 443 440 338 73 BamHI G'GATC_C Cuts at: 2283 3169 3536 2283 Size: 886 367 4586 Fragments arranged by size: 4586 886 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3160 4615 618 Size: 325 217 627 1373 1455 1842 Fragments arranged by size: 1842 1455 1373 627 325 217 BanII G_rGCy'C Cuts at: 1391 2395 2827 3158 1391 Size: 1004 432 331 4072 Fragments arranged by size: 4072 1004 432 331 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2450 2959 928 Size: 971 551 509 3808 Fragments arranged by size: 3808 971 551 509 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4030 4064 1903 Size: 34 2093 34 3678 Fragments arranged by size: 3678 2093 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2591 3731 4941 2591 Size: 1140 1210 3489 Fragments arranged by size: 3489 1210 1140 BclI T'GATC_A Cuts at: 1548 1548 Size: 5839 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4450 136 Size: 107 122 71 631 605 2778 1525 Fragments arranged by size: 2778 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5834 5834 Size: 5839 BlpI GC'TnA_GC Cuts at: 2112 3256 2112 Size: 1144 4695 Bme1580I G_kGCm'C Cuts at: 1790 2395 3900 5056 1790 Size: 605 1505 1156 2573 Fragments arranged by size: 2573 1505 1156 605 BmgBI CAC'GTC Cuts at: 1318 2353 1318 Size: 1035 4804 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3570 4528 442 Size: 416 53 83 399 24 2153 958 1753 Fragments arranged by size: 2153 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5839 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2509 2878 3116 4499 1239 Size: 1270 369 238 1383 2579 Fragments arranged by size: 2579 1383 1270 369 238 Bpu10I CC'TnA_GC Cuts at: 2478 2478 Size: 5839 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2383 2869 3964 4829 5127 2383 Size: 486 1095 865 298 3095 Fragments arranged by size: 3095 1095 865 486 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4502 882 Size: 617 3003 2219 Fragments arranged by size: 3003 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2280 2489 2909 3630 493 Size: 711 1076 209 420 721 2702 Fragments arranged by size: 2702 1076 721 711 420 209 BsaBI GATnn'nnATC Cuts at: 5833 5833 Size: 5839 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2169 2403 2440 2921 Size: 53 83 186 936 636 234 37 481 Cuts at: 2921 3646 4028 4663 275 Size: 725 382 635 1451 Fragments arranged by size: 1451 936 725 636 635 481 382 234 186 83 53 37 BsaWI w'CCGG_w Cuts at: 4272 4670 4944 5091 5502 4272 Size: 398 274 147 411 4609 Fragments arranged by size: 4609 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2762 2792 1178 Size: 30 165 30 1359 30 4225 Fragments arranged by size: 4225 1359 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2906 3113 1186 Size: 1720 207 3912 Fragments arranged by size: 3912 1720 207 BseYI C'CCAG_C Cuts at: 2373 2868 2938 5042 5538 2373 Size: 495 70 2104 496 2674 Fragments arranged by size: 2674 2104 496 495 70 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2572 3149 1163 Size: 573 836 577 3853 Fragments arranged by size: 3853 836 577 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3289 4050 5078 1540 Size: 275 186 1288 761 1028 2301 Fragments arranged by size: 2301 1288 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2864 3900 3985 5056 2864 Size: 1036 85 1071 3647 Fragments arranged by size: 3647 1071 1036 85 BsmI GAATG_Cn' Cuts at: 2745 3350 3443 2745 Size: 605 93 5141 Fragments arranged by size: 5141 605 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2395 2827 2864 3158 3900 3985 5056 Size: 399 605 432 37 294 742 85 1071 Cuts at: 5056 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 605 432 399 294 85 37 BspEI T'CCGG_A Cuts at: 5502 5502 Size: 5839 BspHI T'CATG_A Cuts at: 2347 3728 5458 2347 Size: 1381 1730 2728 Fragments arranged by size: 2728 1730 1381 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2309 2345 3194 844 Size: 1465 36 849 3489 Fragments arranged by size: 3489 1465 849 36 BsrBI CCG'CTC Cuts at: 1394 1823 3726 1394 Size: 429 1903 3507 Fragments arranged by size: 3507 1903 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4334 4508 65 Size: 1183 3086 174 1396 Fragments arranged by size: 3086 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2185 3089 4483 4670 1700 Size: 110 375 904 1394 187 2869 Fragments arranged by size: 2869 1394 904 375 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5839 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5839 BssSI C'ACGA_G Cuts at: 3899 4911 3899 Size: 1012 4827 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5839 BstBI TT'CG_AA Cuts at: 4643 4643 Size: 5839 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5839 BstYI r'GATC_y Cuts at: 1972 2068 2283 3169 3536 3921 3938 5390 5476 Size: 96 215 886 367 385 17 1452 86 Cuts at: 5476 5488 5834 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 886 385 367 346 215 96 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2093 2093 Size: 5839 BtgI C'CryG_G Cuts at: 513 1065 2004 2397 513 Size: 552 939 393 3955 Fragments arranged by size: 3955 939 552 393 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5100 Fragments arranged by size: 5100 683 56 BtsI GCAGTG_nn' Cuts at: 2196 3355 4149 4169 2196 Size: 1159 794 20 3866 Fragments arranged by size: 3866 1159 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5839 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5804 DraI TTT'AAA Cuts at: 3143 3143 Size: 5839 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4846 809 Size: 507 3530 1802 Fragments arranged by size: 3530 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2259 3286 4175 Size: 54 1425 50 275 18 429 1027 889 Cuts at: 4175 5804 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1027 889 429 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3286 1537 Size: 275 1474 4090 Fragments arranged by size: 4090 1474 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2294 3769 1353 Size: 941 1475 3423 Fragments arranged by size: 3423 1475 941 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2932 4426 4800 4946 387 Size: 1064 138 1343 1494 374 146 1280 Fragments arranged by size: 1494 1343 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2509 2708 2878 3116 3135 3902 4499 Size: 393 877 199 170 238 19 767 597 Cuts at: 4499 5286 5554 1239 Size: 787 268 1524 Fragments arranged by size: 1524 877 787 767 597 393 268 238 199 170 19 EcoNI CCTnn'n_nnAGG Cuts at: 2316 2316 Size: 5839 EcoO109I rG'GnC_Cy Cuts at: 2128 2391 2392 2128 Size: 263 1 5575 Fragments arranged by size: 5575 263 1 EcoRI G'AATT_C Cuts at: 2843 3148 3526 2843 Size: 305 378 5156 Fragments arranged by size: 5156 378 305 EcoRV GAT'ATC Cuts at: 2152 2152 Size: 5839 FspI TGC'GCA Cuts at: 4345 4345 Size: 5839 HaeII r_GCGC'y Cuts at: 1678 2856 2875 4986 1678 Size: 1178 19 2111 2531 Fragments arranged by size: 2531 2111 1178 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2498 2530 2614 2646 2762 Size: 32 135 32 926 32 84 32 116 Cuts at: 2762 2794 4480 4512 4554 4586 1373 Size: 32 1686 32 42 32 2626 Fragments arranged by size: 2626 1686 926 135 116 84 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2121 2444 3183 3429 669 Size: 988 281 183 323 739 246 3079 Fragments arranged by size: 3079 988 739 323 281 246 183 HindIII A'AGCT_T Cuts at: 748 748 Size: 5839 HpaI GTT'AAC Cuts at: 3429 3429 Size: 5839 KpnI G_GTAC'C Cuts at: 3164 3164 Size: 5839 MfeI C'AATT_G Cuts at: 3438 3438 Size: 5839 MluI A'CGCG_T Cuts at: 5715 5715 Size: 5839 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4953 5137 1305 Size: 3648 184 2007 Fragments arranged by size: 3648 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2261 10 Size: 54 1425 343 429 3588 Fragments arranged by size: 3588 1425 429 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2552 3797 4156 4315 518 Size: 648 1386 1245 359 159 2042 Fragments arranged by size: 2042 1386 1245 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2373 2399 2529 3255 3932 5080 5325 1606 Size: 767 26 130 726 677 1148 245 2120 Fragments arranged by size: 2120 1148 767 726 677 245 130 26 NaeI GCC'GGC Cuts at: 2187 3091 2187 Size: 904 4935 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4348 Fragments arranged by size: 4348 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5839 NgoMIV G'CCGG_C Cuts at: 2185 3089 2185 Size: 904 4935 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5839 NotI GC'GGCC_GC Cuts at: 3286 3286 Size: 5839 NspI r_CATG'y Cuts at: 1132 1229 3197 4742 1132 Size: 97 1968 1545 2229 Fragments arranged by size: 2229 1968 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5839 PciI A'CATG_T Cuts at: 4738 4738 Size: 5839 PflMI CCAn_nnn'nTGG Cuts at: 1108 2551 1108 Size: 1443 4396 PfoI T'CCnGG_A Cuts at: 2797 2797 Size: 5839 PmeI GTTT'AAAC Cuts at: 3143 3143 Size: 5839 PmlI CAC'GTG Cuts at: 2280 2909 2280 Size: 629 5210 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3908 3940 5421 5453 3908 Size: 32 1481 32 4294 Fragments arranged by size: 4294 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2128 2128 Size: 5839 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5839 PsiI TTA'TAA Cuts at: 3409 3409 Size: 5839 PspOMI G'GGCC_C Cuts at: 2391 2391 Size: 5839 PstI C_TGCA'G Cuts at: 830 2372 2981 3191 830 Size: 1542 609 210 3478 Fragments arranged by size: 3478 1542 609 210 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5839 PvuII CAG'CTG Cuts at: 2373 2373 Size: 5839 SacII CC_GC'GG Cuts at: 2400 2400 Size: 5839 SalI G'TCGA_C Cuts at: 1936 3181 1936 Size: 1245 4594 SbfI CC_TGCA'GG Cuts at: 3191 3191 Size: 5839 ScaI AGT'ACT Cuts at: 4087 5799 4087 Size: 1712 4127 SfcI C'TryA_G Cuts at: 826 1045 2368 2977 3187 4322 5003 5194 826 Size: 219 1323 609 210 1135 681 191 1471 Fragments arranged by size: 1471 1323 1135 681 609 219 210 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5839 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5839 SmaI CCC'GGG Cuts at: 1872 2388 2828 3166 1872 Size: 516 440 338 4545 Fragments arranged by size: 4545 516 440 338 SmlI C'TyrA_G Cuts at: 820 1017 1943 2362 2848 3943 4844 5106 820 Size: 197 926 419 486 1095 901 262 1553 Fragments arranged by size: 1553 1095 926 901 486 419 262 197 SnaBI TAC'GTA Cuts at: 493 3630 493 Size: 3137 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5839 SphI G_CATG'C Cuts at: 1132 3197 1132 Size: 2065 3774 SspI AAT'ATT Cuts at: 5 52 3763 5 Size: 47 3711 2081 Fragments arranged by size: 3711 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5839 StyI C'CwwG_G Cuts at: 513 1065 2004 2312 3278 513 Size: 552 939 308 966 3074 Fragments arranged by size: 3074 966 939 552 308 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2162 2650 3866 3883 4036 4221 4666 Size: 1255 582 488 1216 17 153 185 445 Cuts at: 4666 325 Size: 1498 Fragments arranged by size: 1498 1255 1216 582 488 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2418 2812 3118 Size: 274 80 33 51 1443 441 394 306 Cuts at: 3118 4085 5797 96 Size: 967 1712 138 Fragments arranged by size: 1712 1443 967 441 394 306 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5799 5831 5799 Size: 32 5807 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5839 XbaI T'CTAG_A Cuts at: 3175 3175 Size: 5839 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2277 2277 Size: 5839 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5839 XmaI C'CCGG_G Cuts at: 1870 2386 2826 3164 1870 Size: 516 440 338 4545 Fragments arranged by size: 4545 516 440 338 XmnI GAAnn'nnTTC Cuts at: 3968 3968 Size: 5839 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2404 2922 3647 276 Size: 53 83 186 936 870 518 725 2468 Fragments arranged by size: 2468 936 870 725 518 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI