(Circular) (Six-base) MAPSORT of: pFN21AB5188.seq Check: 2978 from: 1 to: 5971 pFN21AB5188 5971 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2921 3781 278 Size: 53 83 186 936 1385 860 2468 Fragments arranged by size: 2468 1385 936 860 186 83 53 AccI GT'mk_AC Cuts at: 1937 3081 3314 1937 Size: 1144 233 4594 Fragments arranged by size: 4594 1144 233 Acc65I G'GTAC_C Cuts at: 3292 3292 Size: 5971 AclI AA'CG_TT Cuts at: 1563 4098 4471 1563 Size: 2535 373 3063 Fragments arranged by size: 3063 2535 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4034 5418 5686 1632 Size: 2402 1384 268 1917 Fragments arranged by size: 2402 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5774 AflIII A'CryG_T Cuts at: 1203 4870 5847 1203 Size: 3667 977 1327 Fragments arranged by size: 3667 1327 977 AgeI A'CCGG_T Cuts at: 4802 4802 Size: 5971 AhdI GACnn_n'nnGTC Cuts at: 4700 4700 Size: 5971 AleI CACnn'nnGTG Cuts at: 1166 2696 2741 1166 Size: 1530 45 4396 Fragments arranged by size: 4396 1530 45 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2806 2819 2972 1184 Size: 1622 13 153 4183 Fragments arranged by size: 4183 1622 153 13 AlwNI CAG_nnn'CTG Cuts at: 2067 2136 2819 3714 5286 2067 Size: 69 683 895 1572 2752 Fragments arranged by size: 2752 1572 895 683 69 ApaI G_GGCC'C Cuts at: 2719 2719 Size: 5971 ApaLI G'TGCA_C Cuts at: 2557 4028 5184 2557 Size: 1471 1156 3344 Fragments arranged by size: 3344 1471 1156 ApoI r'AATT_y Cuts at: 1493 3280 3506 3658 5767 1493 Size: 1787 226 152 2109 1697 Fragments arranged by size: 2109 1787 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4525 160 Size: 872 3493 1606 Fragments arranged by size: 3493 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 AvaI C'yCGr_G Cuts at: 1870 1943 3296 1870 Size: 73 1353 4545 Fragments arranged by size: 4545 1353 73 BamHI G'GATC_C Cuts at: 2901 3301 3668 2901 Size: 400 367 5204 Fragments arranged by size: 5204 400 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2354 3019 3266 3292 4747 Size: 325 217 627 567 665 247 26 1455 Cuts at: 4747 618 Size: 1842 Fragments arranged by size: 1842 1455 665 627 567 325 247 217 26 BanII G_rGCy'C Cuts at: 1391 2174 2719 3290 1391 Size: 783 545 571 4072 Fragments arranged by size: 4072 783 571 545 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2091 2995 928 Size: 971 192 904 3904 Fragments arranged by size: 3904 971 904 192 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2968 3002 4162 4196 1903 Size: 34 1031 34 1160 34 3678 Fragments arranged by size: 3678 1160 1031 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3863 5073 3863 Size: 1210 4761 BclI T'GATC_A Cuts at: 1548 1548 Size: 5971 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2370 4582 136 Size: 107 122 71 631 605 698 2212 1525 Fragments arranged by size: 2212 1525 698 631 605 122 107 71 BglII A'GATC_T Cuts at: 2476 5966 2476 Size: 3490 2481 BlpI GC'TnA_GC Cuts at: 2173 3388 2173 Size: 1215 4756 Bme1580I G_kGCm'C Cuts at: 1790 2561 2719 3022 4032 5188 1790 Size: 771 158 303 1010 1156 2573 Fragments arranged by size: 2573 1156 1010 771 303 158 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5971 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3040 3702 4660 Size: 416 53 83 399 24 1623 662 958 Cuts at: 4660 442 Size: 1753 Fragments arranged by size: 1753 1623 958 662 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5971 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2188 2193 2188 Size: 5 5966 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2158 4631 1239 Size: 919 2473 2579 Fragments arranged by size: 2579 2473 919 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4096 4961 5259 4096 Size: 865 298 4808 Fragments arranged by size: 4808 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2543 4634 882 Size: 617 1044 2091 2219 Fragments arranged by size: 2219 2091 1044 617 BsaAI yAC'GTr Cuts at: 493 1204 2896 3762 493 Size: 711 1692 866 2702 Fragments arranged by size: 2702 1692 866 711 BsaBI GATnn'nnATC Cuts at: 5965 5965 Size: 5971 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2918 3778 4160 4795 Size: 53 83 186 936 1385 860 382 635 Cuts at: 4795 275 Size: 1451 Fragments arranged by size: 1451 1385 936 860 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2673 4404 4802 5076 5223 5634 2673 Size: 1731 398 274 147 411 3010 Fragments arranged by size: 3010 1731 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2540 2570 2769 2799 1178 Size: 30 165 30 1137 30 199 30 4350 Fragments arranged by size: 4350 1137 199 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2292 2471 3260 1186 Size: 1106 179 789 3897 Fragments arranged by size: 3897 1106 789 179 BseYI C'CCAG_C Cuts at: 2940 3015 5174 5670 2940 Size: 75 2159 496 3241 Fragments arranged by size: 3241 2159 496 75 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2408 2802 1163 Size: 573 672 394 4332 Fragments arranged by size: 4332 672 573 394 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2199 2406 3421 4182 5210 1540 Size: 275 186 198 207 1015 761 1028 2301 Fragments arranged by size: 2301 1028 1015 761 275 207 198 186 BsiHKAI G_wGCw'C Cuts at: 2174 2561 3153 4032 4117 5188 2174 Size: 387 592 879 85 1071 2957 Fragments arranged by size: 2957 1071 879 592 387 85 BsmI GAATG_Cn' Cuts at: 3482 3575 3482 Size: 93 5878 BsmBI CGTCTCn'nnnn_ Cuts at: 3152 3152 Size: 5971 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2174 2561 2719 3022 3153 3290 4032 Size: 399 384 387 158 303 131 137 742 Cuts at: 4032 4117 5188 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 387 384 303 158 137 131 85 BspEI T'CCGG_A Cuts at: 2673 5634 2673 Size: 2961 3010 BspHI T'CATG_A Cuts at: 2505 3860 5590 2505 Size: 1355 1730 2886 Fragments arranged by size: 2886 1730 1355 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3326 844 Size: 2482 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3858 1394 Size: 429 2035 3507 Fragments arranged by size: 3507 2035 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2533 4466 4640 65 Size: 1183 1285 1933 174 1396 Fragments arranged by size: 1933 1396 1285 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2198 4615 4802 1700 Size: 110 388 2417 187 2869 Fragments arranged by size: 2869 2417 388 187 110 BsrGI T'GTAC_A Cuts at: 96 3141 96 Size: 3045 2926 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5971 BssSI C'ACGA_G Cuts at: 4031 5043 4031 Size: 1012 4959 BstAPI GCAn_nnn'nTGC Cuts at: 794 2067 2361 794 Size: 1273 294 4404 Fragments arranged by size: 4404 1273 294 BstBI TT'CG_AA Cuts at: 4775 4775 Size: 5971 BstEII G'GTnAC_C Cuts at: 1184 2971 1184 Size: 1787 4184 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2998 3106 2011 Size: 987 108 4876 Fragments arranged by size: 4876 987 108 BstYI r'GATC_y Cuts at: 1972 2476 2901 3301 3668 4053 4070 5522 5608 Size: 504 425 400 367 385 17 1452 86 Cuts at: 5608 5620 5966 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 504 425 400 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2388 2574 2619 513 Size: 552 1323 186 45 3865 Fragments arranged by size: 3865 1323 552 186 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2971 487 Size: 683 56 1745 3487 Fragments arranged by size: 3487 1745 683 56 BtsI GCAGTG_nn' Cuts at: 2790 3487 4281 4301 2790 Size: 697 794 20 4460 Fragments arranged by size: 4460 794 697 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5971 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5936 DraI TTT'AAA Cuts at: 3275 3275 Size: 5971 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3049 4978 809 Size: 507 1733 1929 1802 Fragments arranged by size: 1929 1802 1733 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2571 2622 2640 Size: 54 1425 50 275 18 741 51 18 Cuts at: 2640 2688 3096 3418 4307 5936 8 Size: 48 408 322 889 1629 43 Fragments arranged by size: 1629 1425 889 741 408 322 275 54 51 50 48 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 3418 1537 Size: 275 1606 4090 Fragments arranged by size: 4090 1606 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2665 3901 1353 Size: 1312 1236 3423 Fragments arranged by size: 3423 1312 1236 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4558 4932 5078 387 Size: 1064 138 2969 374 146 1280 Fragments arranged by size: 2969 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2172 2172 Size: 5971 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2158 4034 4631 5418 5686 1239 Size: 393 526 1876 597 787 268 1524 Fragments arranged by size: 1876 1524 787 597 526 393 268 EcoO109I rG'GnC_Cy Cuts at: 2716 2716 Size: 5971 EcoRI G'AATT_C Cuts at: 3280 3658 3280 Size: 378 5593 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2108 2108 Size: 5971 FspI TGC'GCA Cuts at: 4477 4477 Size: 5971 HaeII r_GCGC'y Cuts at: 1678 2137 5118 1678 Size: 459 2981 2531 Fragments arranged by size: 2981 2531 459 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4612 4644 4686 4718 1373 Size: 32 135 32 3040 32 42 32 2626 Fragments arranged by size: 3040 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3315 3561 669 Size: 988 281 1377 246 3079 Fragments arranged by size: 3079 1377 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5971 HpaI GTT'AAC Cuts at: 3561 3561 Size: 5971 KpnI G_GTAC'C Cuts at: 3296 3296 Size: 5971 MfeI C'AATT_G Cuts at: 3570 3570 Size: 5971 MluI A'CGCG_T Cuts at: 5847 5847 Size: 5971 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5085 5269 1305 Size: 3780 184 2007 Fragments arranged by size: 3780 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2624 2642 2690 10 Size: 54 1425 343 792 18 48 3291 Fragments arranged by size: 3291 1425 792 343 54 48 18 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2336 2573 2696 2741 3929 4288 Size: 648 843 327 237 123 45 1188 359 Cuts at: 4288 4447 518 Size: 159 2042 Fragments arranged by size: 2042 1188 843 648 359 327 237 159 123 45 MspA1I CmG'CkG Cuts at: 1606 2064 3040 3123 3387 4064 5212 5457 1606 Size: 458 976 83 264 677 1148 245 2120 Fragments arranged by size: 2120 1148 976 677 458 264 245 83 NcoI C'CATG_G Cuts at: 513 1065 2574 2619 513 Size: 552 1509 45 3865 Fragments arranged by size: 3865 1509 552 45 NdeI CA'TA_TG Cuts at: 387 387 Size: 5971 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5971 NotI GC'GGCC_GC Cuts at: 3418 3418 Size: 5971 NspI r_CATG'y Cuts at: 1132 1229 2500 3329 4874 1132 Size: 97 1271 829 1545 2229 Fragments arranged by size: 2229 1545 1271 829 97 PasI CC'CwG_GG Cuts at: 1348 3202 1348 Size: 1854 4117 PciI A'CATG_T Cuts at: 4870 4870 Size: 5971 PflMI CCAn_nnn'nTGG Cuts at: 1108 2521 1108 Size: 1413 4558 PfoI T'CCnGG_A Cuts at: 2116 2116 Size: 5971 PmeI GTTT'AAAC Cuts at: 3275 3275 Size: 5971 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4040 4072 5553 5585 4040 Size: 32 1481 32 4426 Fragments arranged by size: 4426 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5971 PsiI TTA'TAA Cuts at: 3541 3541 Size: 5971 PspOMI G'GGCC_C Cuts at: 2715 2715 Size: 5971 PstI C_TGCA'G Cuts at: 830 2910 3199 3323 830 Size: 2080 289 124 3478 Fragments arranged by size: 3478 2080 289 124 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5971 PvuII CAG'CTG Cuts at: 3040 3123 3040 Size: 83 5888 SacI G_AGCT'C Cuts at: 2174 2174 Size: 5971 SalI G'TCGA_C Cuts at: 1936 3313 1936 Size: 1377 4594 SbfI CC_TGCA'GG Cuts at: 3323 3323 Size: 5971 ScaI AGT'ACT Cuts at: 4219 5931 4219 Size: 1712 4259 SfcI C'TryA_G Cuts at: 826 1045 2906 3035 3195 3319 4454 5135 5326 Size: 219 1861 129 160 124 1135 681 191 Cuts at: 5326 826 Size: 1471 Fragments arranged by size: 1861 1471 1135 681 219 191 160 129 124 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5971 SmaI CCC'GGG Cuts at: 1872 3298 1872 Size: 1426 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4075 4976 5238 820 Size: 197 926 2132 901 262 1553 Fragments arranged by size: 2132 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3762 493 Size: 3269 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5971 SphI G_CATG'C Cuts at: 1132 2500 3329 1132 Size: 1368 829 3774 Fragments arranged by size: 3774 1368 829 SspI AAT'ATT Cuts at: 5 52 3895 5 Size: 47 3843 2081 Fragments arranged by size: 3843 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5971 StyI C'CwwG_G Cuts at: 513 1065 2574 2619 3410 513 Size: 552 1509 45 791 3074 Fragments arranged by size: 3074 1509 791 552 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2149 3998 4015 4168 4353 4798 325 Size: 1255 569 1849 17 153 185 445 1498 Fragments arranged by size: 1849 1498 1255 569 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3141 4217 5929 Size: 274 80 33 51 1443 1164 1076 1712 Cuts at: 5929 96 Size: 138 Fragments arranged by size: 1712 1443 1164 1076 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2540 2572 2769 2801 5931 5963 2540 Size: 32 197 32 3130 32 2548 Fragments arranged by size: 3130 2548 197 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5971 XbaI T'CTAG_A Cuts at: 3307 3307 Size: 5971 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2365 2365 Size: 5971 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5971 XmaI C'CCGG_G Cuts at: 1870 3296 1870 Size: 1426 4545 XmnI GAAnn'nnTTC Cuts at: 4100 4100 Size: 5971 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2919 3779 276 Size: 53 83 186 936 1385 860 2468 Fragments arranged by size: 2468 1385 936 860 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI