(Circular) (Six-base) MAPSORT of: pFN21AB5181.seq Check: 9035 from: 1 to: 6673 pFN21AB5181 6673 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4483 278 Size: 53 83 186 936 2947 2468 Fragments arranged by size: 2947 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4016 1937 Size: 2079 4594 Acc65I G'GTAC_C Cuts at: 3191 3994 3191 Size: 803 5870 AclI AA'CG_TT Cuts at: 1563 4800 5173 1563 Size: 3237 373 3063 Fragments arranged by size: 3237 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2621 3156 4736 6120 6388 1632 Size: 989 535 1580 1384 268 1917 Fragments arranged by size: 1917 1580 1384 989 535 268 AfeI AGC'GCT Cuts at: 2819 2819 Size: 6673 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6476 AflIII A'CryG_T Cuts at: 1203 5572 6549 1203 Size: 4369 977 1327 Fragments arranged by size: 4369 1327 977 AgeI A'CCGG_T Cuts at: 5504 5504 Size: 6673 AhdI GACnn_n'nnGTC Cuts at: 5402 5402 Size: 6673 AleI CACnn'nnGTG Cuts at: 1166 2182 2258 2921 1166 Size: 1016 76 663 4918 Fragments arranged by size: 4918 1016 663 76 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3123 3575 3608 3809 1184 Size: 1939 452 33 201 4048 Fragments arranged by size: 4048 1939 452 201 33 AlwNI CAG_nnn'CTG Cuts at: 2780 3242 3358 3447 3841 4416 5988 2780 Size: 462 116 89 394 575 1572 3465 Fragments arranged by size: 3465 1572 575 462 394 116 89 ApaLI G'TGCA_C Cuts at: 4730 5886 4730 Size: 1156 5517 ApoI r'AATT_y Cuts at: 1493 3982 4208 4360 6469 1493 Size: 2489 226 152 2109 1697 Fragments arranged by size: 2489 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5227 160 Size: 872 4195 1606 Fragments arranged by size: 4195 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6673 AvaI C'yCGr_G Cuts at: 1870 1943 2435 3645 3781 3998 1870 Size: 73 492 1210 136 217 4545 Fragments arranged by size: 4545 1210 492 217 136 73 BamHI G'GATC_C Cuts at: 4003 4370 4003 Size: 367 6306 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3047 3191 3994 5449 618 Size: 325 217 627 1260 144 803 1455 1842 Fragments arranged by size: 1842 1455 1260 803 627 325 217 144 BanII G_rGCy'C Cuts at: 1391 2013 2079 2337 2369 2409 2609 3138 3234 Size: 622 66 258 32 40 200 529 96 Cuts at: 3234 3504 3540 3992 1391 Size: 270 36 452 4072 Fragments arranged by size: 4072 622 529 452 270 258 200 96 66 40 36 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2625 3344 928 Size: 971 726 719 4257 Fragments arranged by size: 4257 971 726 719 BbvCI CC'TCA_GC Cuts at: 3823 3823 Size: 6673 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4864 4898 1903 Size: 34 2927 34 3678 Fragments arranged by size: 3678 2927 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4565 5775 4565 Size: 1210 5463 BclI T'GATC_A Cuts at: 1548 2925 1548 Size: 1377 5296 BfrBI ATG'CAT Cuts at: 2274 2274 Size: 6673 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2769 3508 5284 Size: 107 122 71 631 605 1097 739 1776 Cuts at: 5284 136 Size: 1525 Fragments arranged by size: 1776 1525 1097 739 631 605 122 107 71 BglII A'GATC_T Cuts at: 2397 2685 6668 2397 Size: 288 3983 2402 Fragments arranged by size: 3983 2402 288 BlpI GC'TnA_GC Cuts at: 3698 4090 3698 Size: 392 6281 Bme1580I G_kGCm'C Cuts at: 1790 3050 3564 3788 4734 5890 1790 Size: 1260 514 224 946 1156 2573 Fragments arranged by size: 2573 1260 1156 946 514 224 BmgBI CAC'GTC Cuts at: 1318 3673 1318 Size: 2355 4318 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2092 3099 3255 Size: 416 53 83 399 24 675 1007 156 Cuts at: 3255 4404 5362 442 Size: 1149 958 1753 Fragments arranged by size: 1753 1149 1007 958 675 416 399 156 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6673 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2704 2704 Size: 6673 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 3448 3587 5333 1239 Size: 815 1394 139 1746 2579 Fragments arranged by size: 2579 1746 1394 815 139 Bpu10I CC'TnA_GC Cuts at: 2884 3096 3823 2884 Size: 212 727 5734 Fragments arranged by size: 5734 727 212 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4798 5663 5961 4798 Size: 865 298 5510 Fragments arranged by size: 5510 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5336 882 Size: 617 3837 2219 Fragments arranged by size: 3837 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4464 493 Size: 711 3260 2702 Fragments arranged by size: 3260 2702 711 BsaBI GATnn'nnATC Cuts at: 6667 6667 Size: 6673 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4480 4862 5497 275 Size: 53 83 186 936 2947 382 635 1451 Fragments arranged by size: 2947 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3194 5106 5504 5778 5925 6336 3194 Size: 1912 398 274 147 411 3531 Fragments arranged by size: 3531 1912 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6448 Fragments arranged by size: 6448 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2399 2453 2526 2585 2726 2972 1186 Size: 1213 54 73 59 141 246 4887 Fragments arranged by size: 4887 1213 246 141 73 59 54 BseYI C'CCAG_C Cuts at: 3058 3235 3280 3372 3458 3581 3711 5876 6372 Size: 177 45 92 86 123 130 2165 496 Cuts at: 6372 3058 Size: 3359 Fragments arranged by size: 3359 2165 496 177 130 123 92 86 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2678 1163 Size: 573 942 5158 Fragments arranged by size: 5158 942 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2546 4123 4884 5912 1540 Size: 275 186 545 1577 761 1028 2301 Fragments arranged by size: 2301 1577 1028 761 545 275 186 BsiHKAI G_wGCw'C Cuts at: 2079 2337 2409 2609 3002 3138 3540 3704 4734 Size: 258 72 200 393 136 402 164 1030 Cuts at: 4734 4819 5890 2079 Size: 85 1071 2862 Fragments arranged by size: 2862 1071 1030 402 393 258 200 164 136 85 72 BsmI GAATG_Cn' Cuts at: 2707 4184 4277 2707 Size: 1477 93 5103 Fragments arranged by size: 5103 1477 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2079 2337 2369 2409 2609 3002 Size: 399 223 66 258 32 40 200 393 Cuts at: 3002 3050 3138 3234 3504 3540 3564 3704 3788 Size: 48 88 96 270 36 24 140 84 Cuts at: 3788 3992 4734 4819 5890 1391 Size: 204 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 393 270 258 223 204 200 140 96 88 85 84 66 48 40 36 32 24 BspEI T'CCGG_A Cuts at: 6336 6336 Size: 6673 BspHI T'CATG_A Cuts at: 2799 4562 6292 2799 Size: 1763 1730 3180 Fragments arranged by size: 3180 1763 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3435 4028 844 Size: 2591 593 3489 Fragments arranged by size: 3489 2591 593 BsrBI CCG'CTC Cuts at: 1394 1823 2827 4560 1394 Size: 429 1004 1733 3507 Fragments arranged by size: 3507 1733 1004 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5168 5342 65 Size: 1183 3920 174 1396 Fragments arranged by size: 3920 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2981 5317 5504 1700 Size: 110 1171 2336 187 2869 Fragments arranged by size: 2869 2336 1171 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6673 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6673 BssSI C'ACGA_G Cuts at: 4733 5745 4733 Size: 1012 5661 BstAPI GCAn_nnn'nTGC Cuts at: 794 2947 3358 3447 794 Size: 2153 411 89 4020 Fragments arranged by size: 4020 2153 411 89 BstBI TT'CG_AA Cuts at: 5477 5477 Size: 6673 BstEII G'GTnAC_C Cuts at: 1184 3905 1184 Size: 2721 3952 BstXI CCAn_nnnn'nTGG Cuts at: 2184 2263 2362 2587 2184 Size: 79 99 225 6270 Fragments arranged by size: 6270 225 99 79 BstYI r'GATC_y Cuts at: 1972 2166 2397 2685 2892 3016 3031 4003 4370 Size: 194 231 288 207 124 15 972 367 Cuts at: 4370 4755 4772 6224 6310 6322 6668 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 972 385 367 346 288 231 207 194 124 86 17 15 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2540 3900 513 Size: 552 939 536 1360 3286 Fragments arranged by size: 3286 1360 939 552 536 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2030 487 Size: 683 56 804 5130 Fragments arranged by size: 5130 804 683 56 BtsI GCAGTG_nn' Cuts at: 3653 4189 4983 5003 3653 Size: 536 794 20 5323 Fragments arranged by size: 5323 794 536 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6673 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3365 3400 529 Size: 35 2801 35 3802 Fragments arranged by size: 3802 2801 35 35 DraI TTT'AAA Cuts at: 3977 3977 Size: 6673 DraIII CAC_nnn'GTG Cuts at: 3490 3490 Size: 6673 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5680 809 Size: 507 4364 1802 Fragments arranged by size: 4364 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2543 3546 4120 Size: 54 1425 50 275 18 713 1003 574 Cuts at: 4120 5009 6638 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1003 889 713 574 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2543 4120 1537 Size: 275 731 1577 4090 Fragments arranged by size: 4090 1577 731 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2273 2597 2687 2849 3638 4603 1353 Size: 920 324 90 162 789 965 3423 Fragments arranged by size: 3423 965 920 789 324 162 90 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5260 5634 5780 387 Size: 1064 138 3671 374 146 1280 Fragments arranged by size: 3671 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2077 2335 2407 2607 3136 3538 2077 Size: 258 72 200 529 402 5212 Fragments arranged by size: 5212 529 402 258 200 72 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2621 3156 3448 3587 4736 5333 Size: 393 422 567 535 292 139 1149 597 Cuts at: 5333 6120 6388 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1149 787 597 567 535 422 393 292 268 139 EcoNI CCTnn'n_nnAGG Cuts at: 2069 2435 2801 2918 2069 Size: 366 366 117 5824 Fragments arranged by size: 5824 366 366 117 EcoO109I rG'GnC_Cy Cuts at: 2132 2794 3256 3424 3649 3838 2132 Size: 662 462 168 225 189 4967 Fragments arranged by size: 4967 662 462 225 189 168 EcoRI G'AATT_C Cuts at: 3982 4360 3982 Size: 378 6295 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2338 3910 2338 Size: 1572 5101 FspI TGC'GCA Cuts at: 5179 5179 Size: 6673 HaeII r_GCGC'y Cuts at: 1678 2821 3471 3667 5820 1678 Size: 1143 650 196 2153 2531 Fragments arranged by size: 2531 2153 1143 650 196 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2561 2593 2596 2628 3125 Size: 32 135 32 989 32 3 32 497 Cuts at: 3125 3157 5314 5346 5388 5420 1373 Size: 32 2157 32 42 32 2626 Fragments arranged by size: 2626 2157 989 497 135 42 32 32 32 32 32 32 32 3 HincII GTy'rAC Cuts at: 669 1657 1938 2965 4017 4263 669 Size: 988 281 1027 1052 246 3079 Fragments arranged by size: 3079 1052 1027 988 281 246 HindIII A'AGCT_T Cuts at: 748 2504 748 Size: 1756 4917 HpaI GTT'AAC Cuts at: 4263 4263 Size: 6673 KpnI G_GTAC'C Cuts at: 3195 3998 3195 Size: 803 5870 MfeI C'AATT_G Cuts at: 4272 4272 Size: 6673 MluI A'CGCG_T Cuts at: 6549 6549 Size: 6673 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2520 2872 3198 3693 5787 5971 1305 Size: 1215 352 326 495 2094 184 2007 Fragments arranged by size: 2094 2007 1215 495 352 326 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3548 10 Size: 54 1425 343 1716 3135 Fragments arranged by size: 3135 1716 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2182 2258 2744 2921 3557 4631 4990 Size: 648 1016 76 486 177 636 1074 359 Cuts at: 4990 5149 518 Size: 159 2042 Fragments arranged by size: 2042 1074 1016 648 636 486 359 177 159 76 MspA1I CmG'CkG Cuts at: 1606 2418 2780 2944 3309 3447 3743 4089 4766 Size: 812 362 164 365 138 296 346 677 Cuts at: 4766 5914 6159 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 812 677 365 362 346 296 245 164 138 NaeI GCC'GGC Cuts at: 2983 2983 Size: 6673 NcoI C'CATG_G Cuts at: 513 1065 2004 3900 513 Size: 552 939 1896 3286 Fragments arranged by size: 3286 1896 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6673 NgoMIV G'CCGG_C Cuts at: 2981 2981 Size: 6673 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6673 NotI GC'GGCC_GC Cuts at: 4120 4120 Size: 6673 NsiI A_TGCA'T Cuts at: 2276 2276 Size: 6673 NspI r_CATG'y Cuts at: 1132 1229 2274 4031 5576 1132 Size: 97 1045 1757 1545 2229 Fragments arranged by size: 2229 1757 1545 1045 97 PasI CC'CwG_GG Cuts at: 1348 2993 1348 Size: 1645 5028 PciI A'CATG_T Cuts at: 5572 5572 Size: 6673 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6673 PfoI T'CCnGG_A Cuts at: 3420 3420 Size: 6673 PmeI GTTT'AAAC Cuts at: 3977 3977 Size: 6673 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4742 4774 6255 6287 4742 Size: 32 1481 32 5128 Fragments arranged by size: 5128 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2794 3424 3649 3838 2794 Size: 630 225 189 5629 Fragments arranged by size: 5629 630 225 189 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6673 PsiI TTA'TAA Cuts at: 4243 4243 Size: 6673 PstI C_TGCA'G Cuts at: 830 2901 3351 3432 3452 3774 4025 830 Size: 2071 450 81 20 322 251 3478 Fragments arranged by size: 3478 2071 450 322 251 81 20 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6673 PvuII CAG'CTG Cuts at: 2418 2780 2944 3447 3743 2418 Size: 362 164 503 296 5348 Fragments arranged by size: 5348 503 362 296 164 SacI G_AGCT'C Cuts at: 2079 2337 2409 2609 3138 3540 2079 Size: 258 72 200 529 402 5212 Fragments arranged by size: 5212 529 402 258 200 72 SalI G'TCGA_C Cuts at: 1936 4015 1936 Size: 2079 4594 SanDI GG'GwC_CC Cuts at: 3649 3649 Size: 6673 SapI GCTCTTCn'nnn_ Cuts at: 2273 2597 2849 2273 Size: 324 252 6097 Fragments arranged by size: 6097 324 252 SbfI CC_TGCA'GG Cuts at: 2901 4025 2901 Size: 1124 5549 ScaI AGT'ACT Cuts at: 4921 6633 4921 Size: 1712 4961 SfcI C'TryA_G Cuts at: 826 1045 2413 2897 3347 3428 3448 3770 4021 Size: 219 1368 484 450 81 20 322 251 Cuts at: 4021 5156 5837 6028 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1368 1135 681 484 450 322 251 219 191 81 20 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6673 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6673 SmaI CCC'GGG Cuts at: 1872 3647 4000 1872 Size: 1775 353 4545 Fragments arranged by size: 4545 1775 353 SmlI C'TyrA_G Cuts at: 820 1017 1943 4777 5678 5940 820 Size: 197 926 2834 901 262 1553 Fragments arranged by size: 2834 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4464 493 Size: 3971 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6673 SphI G_CATG'C Cuts at: 1132 4031 1132 Size: 2899 3774 SspI AAT'ATT Cuts at: 5 52 4597 5 Size: 47 4545 2081 Fragments arranged by size: 4545 2081 47 StuI AGG'CCT Cuts at: 1517 2201 2459 2882 3027 3511 1517 Size: 684 258 423 145 484 4679 Fragments arranged by size: 4679 684 484 423 258 145 StyI C'CwwG_G Cuts at: 513 1065 2004 2256 3750 3900 4112 513 Size: 552 939 252 1494 150 212 3074 Fragments arranged by size: 3074 1494 939 552 252 212 150 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2933 3848 3893 4700 4717 4870 5055 Size: 1255 1353 915 45 807 17 153 185 Cuts at: 5055 5500 325 Size: 445 1498 Fragments arranged by size: 1498 1353 1255 915 807 445 185 153 45 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4919 6631 96 Size: 274 80 33 51 1443 2942 1712 138 Fragments arranged by size: 2942 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2218 2250 2509 2541 3476 3508 6633 6665 2218 Size: 32 259 32 935 32 3125 32 2226 Fragments arranged by size: 3125 2226 935 259 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6673 XbaI T'CTAG_A Cuts at: 4009 4009 Size: 6673 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2152 3957 2152 Size: 1805 4868 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6673 XmaI C'CCGG_G Cuts at: 1870 3645 3998 1870 Size: 1775 353 4545 Fragments arranged by size: 4545 1775 353 XmnI GAAnn'nnTTC Cuts at: 3206 4802 3206 Size: 1596 5077 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4481 276 Size: 53 83 186 936 2947 2468 Fragments arranged by size: 2947 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI