Restriction map for
pFN21AB5046
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BbsI BbvCI BcgI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsgI BsiEI BsiWI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoT22I FbaI FseI FspI HpaI
KasI KpnI MaeI MaeII MamI
MfeI MluI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SalI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccIII 601 TCCGGA T^CCGGA
AspHI 886 GAGCTC G[AT]GC[AT]^C
AvaI 415 CCCGGG C^[CT]CG[GA]G
BalI 191 TGGCCA TGG^CCA
BanI 1117 GGTGCC G^G[CT][GA]CC
BciVI 91 GTATCC GTATCC(6/5)
BglI 733 GCCCTTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 601 TCCGGA [AT]^CCGG[AT]
BseAI 601 TCCGGA T^CCGGA
BsiHKAI 886 GAGCTC G[AT]GC[AT]^C
BsmAI 1129 GTCTC GTCTC(1/5)
BsmBI 1128 CGTCTC CGTCTC(1/5)
BsmFI 715 GTCCC GGGAC(10/14)
BsmI 915 GAATGC GAATGC(1/-1)
BsoBI 415 CCCGGG C^[CT]CG[GA]G
BspEI 601 TCCGGA T^CCGGA
BsrDI 134 GCAATG GCAATG(2/0)
BssSI 874 CTCGTG CACGAG(-5/-1)
BstEII 960 GGTGACC G^GT[ACGT]ACC
BstPI 960 GGTGACC G^GT[ACGT]ACC
EaeI 191 TGGCCA [CT]^GGCC[GA]
EarI 883 GAAGAG CTCTTC(1/4)
EcoNI 668 CCTTAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 960 GGTGACC G^GT[ACGT]ACC
EcoRV 388 GATATC GAT^ATC
HincII 520 GTCAAC GT[CT]^[GA]AC
HindII 520 GTCAAC GT[CT]^[GA]AC
HindIII 216 AAGCTT A^AGCTT
Ksp632I 883 GAAGAG CTCTTC(1/4)
KspI 972 CCGCGG CCGC^GG
MluNI 191 TGGCCA TGG^CCA
MroI 601 TCCGGA T^CCGGA
MscI 191 TGGCCA TGG^CCA
NspI 57 ACATGC [GA]CATG^[CT]
PleI 1163 GAGTC GAGTC(4/5)
PvuII 1083 CAGCTG CAG^CTG
SacI 886 GAGCTC GAGCT^C
SacII 972 CCGCGG CCGC^GG
SapI 883 GAAGAGC GCTCTTC(1/4)
SmaI 415 CCCGGG CCC^GGG
TfiI 585 GATTC G^A[AT]TC
Tsp509I 552 AATT ^AATT
XmaCI 415 CCCGGG C^CCGGG
XmaI 415 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 829 CGCG CG^CG
973 CGCG
AcyI 745 GGCGTC G[GA]^CG[CT]C
1126 GGCGTC
AfaI 67 GTAC GT^AC
870 GTAC
AlwI 489 GATCC GGATC(4/5)
693 GATCC
BsaHI 745 GGCGTC G[GA]^CG[CT]C
1126 GGCGTC
BstUI 829 CGCG CG^CG
973 CGCG
BstYI 488 AGATCC [GA]^GATC[CT]
692 AGATCC
DdeI 252 CTCAG C^T[ACGT]AG
1140 CTCAG
DsaI 62 CCGTGG C^C[GA][CT]GG
972 CCGCGG
EcoT14I 533 CCAAGG C^C[AT][AT]GG
570 CCAAGG
HaeII 314 AGCGCC [GA]GCGC^[CT]
436 AGCGCC
Hin1I 745 GGCGTC G[GA]^CG[CT]C
1126 GGCGTC
HinfI 585 GATTC G^A[ACGT]TC
1163 GAGTC
MboII 657 GAAGA GAAGA(8/7)
883 GAAGA
MflI 488 AGATCC [GA]^GATC[CT]
692 AGATCC
MseI 554 TTAA T^TAA
670 TTAA
MvnI 829 CGCG CG^CG
973 CGCG
RsaI 67 GTAC GT^AC
870 GTAC
StyI 533 CCAAGG C^C[AT][AT]GG
570 CCAAGG
Tru9I 554 TTAA T^TAA
670 TTAA
TspRI 83 CACTG CA[GC]TG[ACGT][ACGT]^
206 CACTG
XcmI 533 CCAAGGAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
728 CCAGGGCCCTTGTGG
XhoII 488 AGATCC [GA]^GATC[CT]
692 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AlwNI ApaI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DpnII DraII
DrdI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HpaII HphI ItaI MaeIII
MboI MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
SmlI TseI Tsp45I