Restriction map for
pFN21AB5046
(Circular) (Six-base) MAPSORT of: pFN21AB5046.seq Check: 6518 from: 1 to: 5893
pFN21AB5046 5893 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3703 278
Size: 53 83 186 936 2167 2468
Fragments arranged by size:
2468 2167 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3236 1937
Size: 1299 4594
Acc65I G'GTAC_C
Cuts at: 3214 3214
Size: 5893
AclI AA'CG_TT
Cuts at: 1563 4020 4393 1563
Size: 2457 373 3063
Fragments arranged by size:
3063 2457 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2189 2528 3956 5340 5608 1632
Size: 557 339 1428 1384 268 1917
Fragments arranged by size:
1917 1428 1384 557 339 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5696
AflIII A'CryG_T
Cuts at: 1203 4792 5769 1203
Size: 3589 977 1327
Fragments arranged by size:
3589 1327 977
AgeI A'CCGG_T
Cuts at: 4724 4724
Size: 5893
AhdI GACnn_n'nnGTC
Cuts at: 4622 4622
Size: 5893
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5893
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5893
AlwNI CAG_nnn'CTG
Cuts at: 2212 2631 2701 3082 3636 5208 2212
Size: 419 70 381 554 1572 2897
Fragments arranged by size:
2897 1572 554 419 381 70
ApaI G_GGCC'C
Cuts at: 2380 2740 2977 2380
Size: 360 237 5296
Fragments arranged by size:
5296 360 237
ApaLI G'TGCA_C
Cuts at: 3950 5106 3950
Size: 1156 4737
ApoI r'AATT_y
Cuts at: 1493 3202 3428 3580 5689 1493
Size: 1709 226 152 2109 1697
Fragments arranged by size:
2109 1709 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4447 160
Size: 872 3415 1606
Fragments arranged by size:
3415 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
AvaI C'yCGr_G
Cuts at: 1870 1943 2420 3218 1870
Size: 73 477 798 4545
Fragments arranged by size:
4545 798 477 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2158 2191 2158
Size: 33 5860
BamHI G'GATC_C
Cuts at: 3223 3590 3223
Size: 367 5526
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3122 3214 4669 618
Size: 325 217 627 1335 92 1455 1842
Fragments arranged by size:
1842 1455 1335 627 325 217 92
BanII G_rGCy'C
Cuts at: 1391 2380 2438 2644 2740 2895 2977 3212 1391
Size: 989 58 206 96 155 82 235 4072
Fragments arranged by size:
4072 989 235 206 155 96 82 58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4922
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4084 4118 1903
Size: 34 2147 34 3678
Fragments arranged by size:
3678 2147 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2107 3785 4995 2107
Size: 1678 1210 3005
Fragments arranged by size:
3005 1678 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5893
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2744 4504 136
Size: 107 122 71 631 605 1072 1760 1525
Fragments arranged by size:
1760 1525 1072 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5888 5888
Size: 5893
BlpI GC'TnA_GC
Cuts at: 3310 3310
Size: 5893
Bme1580I G_kGCm'C
Cuts at: 1790 2380 2740 2977 3127 3954 5110 1790
Size: 590 360 237 150 827 1156 2573
Fragments arranged by size:
2573 1156 827 590 360 237 150
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5893
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3624 4582 442
Size: 416 53 83 399 24 2207 958 1753
Fragments arranged by size:
2207 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5893
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2660 2660
Size: 5893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 2422 2678 2843 3068 4553 1239
Size: 1010 173 256 165 225 1485 2579
Fragments arranged by size:
2579 1485 1010 256 225 173 165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2128 2753 3020 4018 4883 5181 2128
Size: 625 267 998 865 298 2840
Fragments arranged by size:
2840 998 865 625 298 267
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4556 882
Size: 617 3057 2219
Fragments arranged by size:
3057 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3684 493
Size: 711 2480 2702
Fragments arranged by size:
2702 2480 711
BsaBI GATnn'nnATC
Cuts at: 5887 5887
Size: 5893
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2751 3132 3700 4082
Size: 53 83 186 936 1218 381 568 382
Cuts at: 4082 4717 275
Size: 635 1451
Fragments arranged by size:
1451 1218 936 635 568 382 381 186
83 53
BsaWI w'CCGG_w
Cuts at: 2606 4326 4724 4998 5145 5556 2606
Size: 1720 398 274 147 411 2943
Fragments arranged by size:
2943 1720 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5668
Fragments arranged by size:
5668 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2504 2652 3023 1186
Size: 1318 148 371 4056
Fragments arranged by size:
4056 1318 371 148
BseYI C'CCAG_C
Cuts at: 2839 2860 5096 5592 2839
Size: 21 2236 496 3140
Fragments arranged by size:
3140 2236 496 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5320
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3343 4104 5132 1540
Size: 275 186 1342 761 1028 2301
Fragments arranged by size:
2301 1342 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2895 3954 4039 5110 2895
Size: 1059 85 1071 3678
Fragments arranged by size:
3678 1071 1059 85
BsmI GAATG_Cn'
Cuts at: 2926 3404 3497 2926
Size: 478 93 5322
Fragments arranged by size:
5322 478 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3139 3139
Size: 5893
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2380 2438 2644 2740 2895 2977 3127
Size: 399 590 58 206 96 155 82 150
Cuts at: 3127 3212 3954 4039 5110 1391
Size: 85 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 590 399 206 155 150
96 85 85 82 58
BspEI T'CCGG_A
Cuts at: 2606 5556 2606
Size: 2950 2943
BspHI T'CATG_A
Cuts at: 3782 5512 3782
Size: 1730 4163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3248 844
Size: 2404 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3780 1394
Size: 429 1957 3507
Fragments arranged by size:
3507 1957 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2146 4388 4562 65
Size: 1183 898 2242 174 1396
Fragments arranged by size:
2242 1396 1183 898 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4537 4724 1700
Size: 110 2727 187 2869
Fragments arranged by size:
2869 2727 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5893
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5893
BssSI C'ACGA_G
Cuts at: 2879 3953 4965 2879
Size: 1074 1012 3807
Fragments arranged by size:
3807 1074 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5893
BstBI TT'CG_AA
Cuts at: 4697 4697
Size: 5893
BstEII G'GTnAC_C
Cuts at: 1184 2965 1184
Size: 1781 4112
BstXI CCAn_nnnn'nTGG
Cuts at: 2141 2821 3157 2141
Size: 680 336 4877
Fragments arranged by size:
4877 680 336
BstYI r'GATC_y
Cuts at: 1972 2493 2697 3223 3590 3975 3992 5444 5530
Size: 521 204 526 367 385 17 1452 86
Cuts at: 5530 5542 5888 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 526 521 385 367 346 204
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2067 2977 513
Size: 552 1002 910 3429
Fragments arranged by size:
3429 1002 910 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5154
Fragments arranged by size:
5154 683 56
BtsI GCAGTG_nn'
Cuts at: 3409 4203 4223 3409
Size: 794 20 5079
Fragments arranged by size:
5079 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5893
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5858
DraI TTT'AAA
Cuts at: 3197 3197
Size: 5893
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2309 2621 3167 4900 809
Size: 507 993 312 546 1733 1802
Fragments arranged by size:
1802 1733 993 546 507 312
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2196 3340 4229
Size: 54 1425 50 275 18 366 1144 889
Cuts at: 4229 5858 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1144 889 366 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3340 1537
Size: 275 1528 4090
Fragments arranged by size:
4090 1528 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2883 3823 1353
Size: 1530 940 3423
Fragments arranged by size:
3423 1530 940
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4480 4854 5000 387
Size: 1064 138 2891 374 146 1280
Fragments arranged by size:
2891 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2893 2893
Size: 5893
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2249 2422 2528 2678 2843 3068
Size: 393 557 60 173 106 150 165 225
Cuts at: 3068 3956 4553 5340 5608 1239
Size: 888 597 787 268 1524
Fragments arranged by size:
1524 888 787 597 557 393 268 225
173 165 150 106 60
EcoNI CCTnn'n_nnAGG
Cuts at: 2677 2677
Size: 5893
EcoO109I rG'GnC_Cy
Cuts at: 2376 2377 2736 2737 2973 3079 2376
Size: 1 359 1 236 106 5190
Fragments arranged by size:
5190 359 236 106 1 1
EcoRI G'AATT_C
Cuts at: 3202 3580 3202
Size: 378 5515
EcoRV GAT'ATC
Cuts at: 2395 2395
Size: 5893
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2066 2587 2066
Size: 521 5372
FspI TGC'GCA
Cuts at: 4399 4399
Size: 5893
HaeII r_GCGC'y
Cuts at: 1678 2323 2445 5040 1678
Size: 645 122 2595 2531
Fragments arranged by size:
2595 2531 645 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2801 2833 4534 4566 4608
Size: 32 135 32 1229 32 1701 32 42
Cuts at: 4608 4640 1373
Size: 32 2626
Fragments arranged by size:
2626 1701 1229 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2527 3237 3483 669
Size: 988 281 589 710 246 3079
Fragments arranged by size:
3079 988 710 589 281 246
HindIII A'AGCT_T
Cuts at: 748 2221 748
Size: 1473 4420
HpaI GTT'AAC
Cuts at: 3483 3483
Size: 5893
KpnI G_GTAC'C
Cuts at: 3218 3218
Size: 5893
MfeI C'AATT_G
Cuts at: 3492 3492
Size: 5893
MluI A'CGCG_T
Cuts at: 5769 5769
Size: 5893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2388 5007 5191 1305
Size: 1083 2619 184 2007
Fragments arranged by size:
2619 2007 1083 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2198 10
Size: 54 1425 343 366 3705
Fragments arranged by size:
3705 1425 366 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2139 2900 2919 3851 4210 4369 518
Size: 648 973 761 19 932 359 159 2042
Fragments arranged by size:
2042 973 932 761 648 359 159 19
MspA1I CmG'CkG
Cuts at: 1606 2506 2979 3090 3309 3986 5134 5379 1606
Size: 900 473 111 219 677 1148 245 2120
Fragments arranged by size:
2120 1148 900 677 473 245 219 111
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5341
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5893
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5893
NotI GC'GGCC_GC
Cuts at: 3340 3340
Size: 5893
NspI r_CATG'y
Cuts at: 1132 1229 2066 3251 4796 1132
Size: 97 837 1185 1545 2229
Fragments arranged by size:
2229 1545 1185 837 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5893
PciI A'CATG_T
Cuts at: 4792 4792
Size: 5893
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5893
PmeI GTTT'AAAC
Cuts at: 3197 3197
Size: 5893
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3962 3994 5475 5507 3962
Size: 32 1481 32 4348
Fragments arranged by size:
4348 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5893
PsiI TTA'TAA
Cuts at: 3463 3463
Size: 5893
PspOMI G'GGCC_C
Cuts at: 2376 2736 2973 2376
Size: 360 237 5296
Fragments arranged by size:
5296 360 237
PstI C_TGCA'G
Cuts at: 830 3245 830
Size: 2415 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5893
PvuII CAG'CTG
Cuts at: 3090 3090
Size: 5893
SacI G_AGCT'C
Cuts at: 2895 2895
Size: 5893
SacII CC_GC'GG
Cuts at: 2980 2980
Size: 5893
SalI G'TCGA_C
Cuts at: 1936 3235 1936
Size: 1299 4594
SapI GCTCTTCn'nnn_
Cuts at: 2883 2883
Size: 5893
SbfI CC_TGCA'GG
Cuts at: 3245 3245
Size: 5893
ScaI AGT'ACT
Cuts at: 4141 5853 4141
Size: 1712 4181
SfcI C'TryA_G
Cuts at: 826 1045 3241 4376 5057 5248 826
Size: 219 2196 1135 681 191 1471
Fragments arranged by size:
2196 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5893
SmaI CCC'GGG
Cuts at: 1872 2422 3220 1872
Size: 550 798 4545
Fragments arranged by size:
4545 798 550
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2107 2768 3035 3997 4898 5160
Size: 197 926 164 661 267 962 901 262
Cuts at: 5160 820
Size: 1553
Fragments arranged by size:
1553 962 926 901 661 267 262 197
164
SnaBI TAC'GTA
Cuts at: 493 3684 493
Size: 3191 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5893
SphI G_CATG'C
Cuts at: 1132 3251 1132
Size: 2119 3774
SspI AAT'ATT
Cuts at: 5 52 3817 5
Size: 47 3765 2081
Fragments arranged by size:
3765 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5893
StyI C'CwwG_G
Cuts at: 513 1065 2538 2575 3332 513
Size: 552 1473 37 757 3074
Fragments arranged by size:
3074 1473 757 552 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2266 3920 3937 4090 4275 4720 325
Size: 1255 686 1654 17 153 185 445 1498
Fragments arranged by size:
1654 1498 1255 686 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4139 5851 96
Size: 274 80 33 51 1443 2162 1712 138
Fragments arranged by size:
2162 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5853 5885 5853
Size: 32 5861
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5893
XbaI T'CTAG_A
Cuts at: 3229 3229
Size: 5893
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2545 2740 2545
Size: 195 5698
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5893
XmaI C'CCGG_G
Cuts at: 1870 2420 3218 1870
Size: 550 798 4545
Fragments arranged by size:
4545 798 550
XmnI GAAnn'nnTTC
Cuts at: 4022 4022
Size: 5893
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3701 276
Size: 53 83 186 936 2167 2468
Fragments arranged by size:
2468 2167 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I Bsu36I DraIII FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI RsrII SanDI SexAI SfiI SfoI
SrfI SwaI