(Circular) (Six-base) MAPSORT of: pFN21AB4977.seq Check: 4242 from: 1 to: 5905 pFN21AB4977 5905 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3715 278 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2024 3248 1937 Size: 87 1224 4594 Fragments arranged by size: 4594 1224 87 Acc65I G'GTAC_C Cuts at: 3226 3226 Size: 5905 AclI AA'CG_TT Cuts at: 1563 4032 4405 1563 Size: 2469 373 3063 Fragments arranged by size: 3063 2469 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3968 5352 5620 1632 Size: 2336 1384 268 1917 Fragments arranged by size: 2336 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5708 AflIII A'CryG_T Cuts at: 1203 4804 5781 1203 Size: 3601 977 1327 Fragments arranged by size: 3601 1327 977 AgeI A'CCGG_T Cuts at: 4736 4736 Size: 5905 AhdI GACnn_n'nnGTC Cuts at: 4634 4634 Size: 5905 AleI CACnn'nnGTG Cuts at: 1166 2678 1166 Size: 1512 4393 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5905 AlwNI CAG_nnn'CTG Cuts at: 2071 3648 5220 2071 Size: 1577 1572 2756 Fragments arranged by size: 2756 1577 1572 ApaLI G'TGCA_C Cuts at: 3962 5118 3962 Size: 1156 4749 ApoI r'AATT_y Cuts at: 1493 2594 3214 3440 3592 5701 1493 Size: 1101 620 226 152 2109 1697 Fragments arranged by size: 2109 1697 1101 620 226 152 AseI AT'TA_AT Cuts at: 160 1032 4459 160 Size: 872 3427 1606 Fragments arranged by size: 3427 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 AvaI C'yCGr_G Cuts at: 1870 1943 3230 1870 Size: 73 1287 4545 Fragments arranged by size: 4545 1287 73 BamHI G'GATC_C Cuts at: 3235 3602 3235 Size: 367 5538 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3226 4681 618 Size: 325 217 627 1439 1455 1842 Fragments arranged by size: 1842 1455 1439 627 325 217 BanII G_rGCy'C Cuts at: 1391 3224 1391 Size: 1833 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4934 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4096 4130 1903 Size: 34 2159 34 3678 Fragments arranged by size: 3678 2159 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3797 5007 3797 Size: 1210 4695 BclI T'GATC_A Cuts at: 1548 1548 Size: 5905 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4516 136 Size: 107 122 71 631 605 2844 1525 Fragments arranged by size: 2844 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5900 5900 Size: 5905 BlpI GC'TnA_GC Cuts at: 2072 3322 2072 Size: 1250 4655 Bme1580I G_kGCm'C Cuts at: 1790 2709 3966 5122 1790 Size: 919 1257 1156 2573 Fragments arranged by size: 2573 1257 1156 919 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5905 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3033 3636 4594 Size: 416 53 83 399 24 1616 603 958 Cuts at: 4594 442 Size: 1753 Fragments arranged by size: 1753 1616 958 603 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5905 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2502 2502 Size: 5905 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2127 4565 1239 Size: 888 2438 2579 Fragments arranged by size: 2579 2438 888 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2252 4030 4895 5193 2252 Size: 1778 865 298 2964 Fragments arranged by size: 2964 1778 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4568 882 Size: 617 3069 2219 Fragments arranged by size: 3069 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2419 3696 493 Size: 711 1215 1277 2702 Fragments arranged by size: 2702 1277 1215 711 BsaBI GATnn'nnATC Cuts at: 2613 2736 2844 5899 2613 Size: 123 108 3055 2619 Fragments arranged by size: 3055 2619 123 108 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3712 4094 4729 275 Size: 53 83 186 936 2179 382 635 1451 Fragments arranged by size: 2179 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2400 4338 4736 5010 5157 5568 2400 Size: 1938 398 274 147 411 2737 Fragments arranged by size: 2737 1938 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5680 Fragments arranged by size: 5680 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5905 BseYI C'CCAG_C Cuts at: 5108 5604 5108 Size: 496 5409 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5332 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3355 4116 5144 1540 Size: 275 186 1354 761 1028 2301 Fragments arranged by size: 2301 1354 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3966 4051 5122 3966 Size: 85 1071 4749 Fragments arranged by size: 4749 1071 85 BsmI GAATG_Cn' Cuts at: 2824 3416 3509 2824 Size: 592 93 5220 Fragments arranged by size: 5220 592 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2103 2103 Size: 5905 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2709 3224 3966 4051 5122 1391 Size: 399 919 515 742 85 1071 2174 Fragments arranged by size: 2174 1071 919 742 515 399 85 BspEI T'CCGG_A Cuts at: 5568 5568 Size: 5905 BspHI T'CATG_A Cuts at: 3794 5524 3794 Size: 1730 4175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3260 844 Size: 2416 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3792 1394 Size: 429 1969 3507 Fragments arranged by size: 3507 1969 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4400 4574 65 Size: 1183 3152 174 1396 Fragments arranged by size: 3152 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2032 4549 4736 1700 Size: 110 222 2517 187 2869 Fragments arranged by size: 2869 2517 222 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5905 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5905 BssSI C'ACGA_G Cuts at: 3965 4977 3965 Size: 1012 4893 BstAPI GCAn_nnn'nTGC Cuts at: 794 3130 794 Size: 2336 3569 BstBI TT'CG_AA Cuts at: 4709 4709 Size: 5905 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5905 BstYI r'GATC_y Cuts at: 1972 2329 2504 2608 3187 3235 3602 3987 4004 Size: 357 175 104 579 48 367 385 17 Cuts at: 4004 5456 5542 5554 5900 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 579 385 367 357 346 175 104 86 48 17 12 BtgI C'CryG_G Cuts at: 513 1065 2569 513 Size: 552 1504 3849 Fragments arranged by size: 3849 1504 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2956 487 Size: 683 56 1730 3436 Fragments arranged by size: 3436 1730 683 56 BtsI GCAGTG_nn' Cuts at: 2083 2552 3421 4215 4235 2083 Size: 469 869 794 20 3753 Fragments arranged by size: 3753 869 794 469 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5905 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5870 DraI TTT'AAA Cuts at: 3209 3209 Size: 5905 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4912 809 Size: 507 3596 1802 Fragments arranged by size: 3596 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2566 3352 Size: 54 1425 50 275 18 225 511 786 Cuts at: 3352 4241 5870 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 786 511 275 225 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3352 1537 Size: 275 1540 4090 Fragments arranged by size: 4090 1540 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3835 1353 Size: 2482 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4492 4866 5012 387 Size: 1064 138 2903 374 146 1280 Fragments arranged by size: 2903 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2127 3968 4565 5352 5620 1239 Size: 393 495 1841 597 787 268 1524 Fragments arranged by size: 1841 1524 787 597 495 393 268 EcoRI G'AATT_C Cuts at: 3214 3592 3214 Size: 378 5527 FspI TGC'GCA Cuts at: 4411 4411 Size: 5905 HaeII r_GCGC'y Cuts at: 1678 5052 1678 Size: 3374 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2515 2547 2659 2691 4546 Size: 32 135 32 943 32 112 32 1855 Cuts at: 4546 4578 4620 4652 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1855 943 135 112 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2025 3249 3495 669 Size: 988 281 87 1224 246 3079 Fragments arranged by size: 3079 1224 988 281 246 87 HindIII A'AGCT_T Cuts at: 748 748 Size: 5905 HpaI GTT'AAC Cuts at: 3495 3495 Size: 5905 KpnI G_GTAC'C Cuts at: 3230 3230 Size: 5905 MfeI C'AATT_G Cuts at: 3504 3504 Size: 5905 MluI A'CGCG_T Cuts at: 5781 5781 Size: 5905 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5019 5203 1305 Size: 3714 184 2007 Fragments arranged by size: 3714 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2568 10 Size: 54 1425 343 736 3347 Fragments arranged by size: 3347 1425 736 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2574 2678 2712 3863 4222 4381 518 Size: 648 1408 104 34 1151 359 159 2042 Fragments arranged by size: 2042 1408 1151 648 359 159 104 34 MspA1I CmG'CkG Cuts at: 1606 3321 3998 5146 5391 1606 Size: 1715 677 1148 245 2120 Fragments arranged by size: 2120 1715 1148 677 245 NaeI GCC'GGC Cuts at: 2034 2034 Size: 5905 NcoI C'CATG_G Cuts at: 513 1065 2569 513 Size: 552 1504 3849 Fragments arranged by size: 3849 1504 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5905 NgoMIV G'CCGG_C Cuts at: 2032 2032 Size: 5905 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5905 NotI GC'GGCC_GC Cuts at: 3352 3352 Size: 5905 NspI r_CATG'y Cuts at: 1132 1229 2050 3263 4808 1132 Size: 97 821 1213 1545 2229 Fragments arranged by size: 2229 1545 1213 821 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5905 PciI A'CATG_T Cuts at: 4804 4804 Size: 5905 PflMI CCAn_nnn'nTGG Cuts at: 1108 2294 1108 Size: 1186 4719 PmeI GTTT'AAAC Cuts at: 3209 3209 Size: 5905 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2703 2735 3974 4006 5487 5519 2703 Size: 32 1239 32 1481 32 3089 Fragments arranged by size: 3089 1481 1239 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5905 PsiI TTA'TAA Cuts at: 2844 3475 2844 Size: 631 5274 PstI C_TGCA'G Cuts at: 830 3257 830 Size: 2427 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5905 SalI G'TCGA_C Cuts at: 1936 2023 3247 1936 Size: 87 1224 4594 Fragments arranged by size: 4594 1224 87 SbfI CC_TGCA'GG Cuts at: 3257 3257 Size: 5905 ScaI AGT'ACT Cuts at: 4153 5865 4153 Size: 1712 4193 SfcI C'TryA_G Cuts at: 826 1045 2349 2496 3253 4388 5069 5260 826 Size: 219 1304 147 757 1135 681 191 1471 Fragments arranged by size: 1471 1304 1135 757 681 219 191 147 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 SgrAI Cr'CCGG_yG Cuts at: 1700 2032 1700 Size: 332 5573 SmaI CCC'GGG Cuts at: 1872 3232 1872 Size: 1360 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2267 4009 4910 5172 820 Size: 197 926 324 1742 901 262 1553 Fragments arranged by size: 1742 1553 926 901 324 262 197 SnaBI TAC'GTA Cuts at: 493 3696 493 Size: 3203 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5905 SphI G_CATG'C Cuts at: 1132 3263 1132 Size: 2131 3774 SspI AAT'ATT Cuts at: 5 52 2243 2688 3829 5 Size: 47 2191 445 1141 2081 Fragments arranged by size: 2191 2081 1141 445 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5905 StyI C'CwwG_G Cuts at: 513 1065 2569 3201 3344 513 Size: 552 1504 632 143 3074 Fragments arranged by size: 3074 1504 632 552 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2735 3932 3949 4102 4287 4732 325 Size: 1255 1155 1197 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1197 1155 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4151 5863 96 Size: 274 80 33 51 1443 2174 1712 138 Fragments arranged by size: 2174 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5865 5897 5865 Size: 32 5873 Tth111I GACn'n_nGTC Cuts at: 1316 2126 1316 Size: 810 5095 XbaI T'CTAG_A Cuts at: 2507 3241 2507 Size: 734 5171 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2291 2291 Size: 5905 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5905 XmaI C'CCGG_G Cuts at: 1870 3230 1870 Size: 1360 4545 XmnI GAAnn'nnTTC Cuts at: 4034 4034 Size: 5905 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3713 276 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI